质粒名称: | pKW08-Lx-Int |
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出品公司: | 爱迪基因 |
目录编号: | 25015 |
存储实验室: | Brian Robertson |
载体骨架基本信息 |
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载体名称: | pMC1M |
载体出品公司: | -- |
载体抗性: | 潮霉素 |
空载体大小: | -- |
完整质粒大小: | 8535 bp |
载体修饰: | -- |
载体类型: | 细菌表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | pKW08-Lx-Int |
物种来源: | -- |
插入大小: | 8535 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pKW08-Lx-Int was a gift from Brian Robertson (Addgene plasmid # 25015 ; http://n2t.net/addgene:25015 ; RRID:Addgene_25015) |
参考文献部分: | Improved mycobacterial tetracycline inducible vectors. Williams KJ, Joyce G, Robertson BD. Plasmid. 2010 Apr 29. ():. 10.1016/j.plasmid.2010.04.003 PubMed 20434484 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pKW08-Lx-Int 8535 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pKW08-Lx-Int SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 8535) AUTHORS Williams KJ, Joyce G, Robertson BD TITLE Improved mycobacterial tetracycline inducible vectors. JOURNAL Plasmid. 2010 Apr 29. ():. PUBMED 20434484 REFERENCE 2 (bases 1 to 8535) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..8535 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 14..41 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind complement(2903..2919) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2904..2920) /label=pBluescriptKS /note="For pBluescript vector" terminator 3693..3739 /label=rrnB T1 terminator /note="transcription terminator T1 from the E. coli rrnB gene" rep_origin 7700..8288 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 8189..8208 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 8442..8459 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa 61 acctctagat cacgattcgc tcgaggtcaa tgacgctgtg cgggagtgag agaatccgtc 121 gatcagtgct gtcaggccga aatcgaattc ccggtcgcgt tcaggaacgc tcgcattgcc 181 gacctctgcg gtttgctcct ccagtacgac accgacggtg tatcggctga ccgcgatgag 241 catgctgatg gctgtctgtt catcgaatcc ctgagacacg agaagcccga cttgctgacc 301 ggaagcctca gctccttcgt cggtgtcgtc gccggatggt ctgcggtggt gctcagcgtg 361 gagacgcgca ccgtcacgga ccgcgtgtag tgcgtggcgg aaacttcttg cgtttcgcaa 421 gagaaatgcg tcccatttct cgtcggactc ggggaaggaa gcgtgatgct ctcggtcaag 481 cacgtcgctc gccagcgctg cgaggagttc ggccttcgtg cggaagtgcc agtagaggcc 541 gggctgctgt acctgtaagt gagccgccag cgcgcgagtg gtgaagccat cgagcccagt 601 ctcgtcgagc acctgccggg ccccgagcaa cacggacgtg cggtcgagac gcttccggtg 661 gtgagtcata gttgcacttt atcatcgata actttatctt agataaagtg actgctcgct 721 actctcatct gactgctcgc tactctcatc gtggaatcct gacaggatcc ggaggaaatg 781 ttatgaaatt tggaaacttc ctcctcactt atcagccacc tgagctatct cagaccgaag 841 tgatgaagcg attggttaat ctgggcaaag cgtctgaagg ttgtggcttc gacaccgttt 901 ggttgctaga gcaccacttc actgaatttg ggttgttagg gaatccttat gttgctgccg 961 cacacctatt aggtgcgaca gaaacgctca acgttggcac tgcagctatc gtattgccga 1021 ctgcccatcc ggttcgacaa gcagaagacg taaacctact ggatcaaatg tcaaaaggac 1081 gattccgttt tggtatttgt cgcggtttgt acgataaaga ttttcgtgtc tttggtacag 1141 acatggataa cagccgagcc ttaatggact gttggtatga cttgatgaaa gaaggcttca 1201 atgaaggcta tatcgcggcg gataacgaac atattaagtt cccgaaaatc caactgaatc 1261 catcggctta cacacaaggt ggtgctcctg tttatgtcgt cgcggagtca gcatcaacga 1321 cagaatgggc tgcagagcgt ggcctaccaa tgattctaag ctggatcatc aacactcacg 1381 agaagaaagc gcagcttgat ctttacaacg aagtcgcgac tgaacatggc tacgatgtga 1441 ctaagattga ccactgtttg tcttacatca cctccgtcga tcatgactca aatagagcca 1501 aagatatttg ccgcaacttc ttgggccatt ggtacgactc atacgtgaat gccaccaaga 1561 tttttgacga ctctgaccaa acaaaaggtt acgacttcaa taaaggtcaa tggcgtgatt 1621 ttgtgttgaa aggccacaaa gacaccaatc gccgaattga ttacagctac gaaatcaacc 1681 cagtagggac gcctgaagag tgtatcgcga ttatccagca agatattgat gcgacgggta 1741 ttgacaatat ttgttgtggt tttgaagcaa acggttctga agaagaaatt atcgcatcta 1801 tgaagctatt ccagtctgat gtgatgccat atctcaaaga aaaacagtaa ttaatatttt 1861 ctaaaaggaa agagacatga aatttggatt attcttcctc aattttatga actcaaagcg 1921 ttcttctgat caagtcatcg aagaaatgtt agataccgca cattacgtag atcagttgaa 1981 gtttgacacg ttggctgttt acgaaaacca tttctcgaac aatggtgtgg ttggtgcccc 2041 actaacagtg gctggttttt tacttggtat gacaaagaac gccaaagtgg cttcgttgaa 2101 tcacgtcatt accacgcatc atccagtacg tgtggcggaa gaagcgtgtc tacttgacca 2161 aatgagtgaa ggccgttttg cctttggctt tagtgattgt gaaaagagtg cagatatgcg 2221 cttctttaat cgaccaacgg attctcagtt tcagttgttc agtgagtgtc acaagatcat 2281 caatgattca ttcactactg ggtactgcca tccaaacaat gatttttata gttttcctaa 2341 aatctccgtt aacccacacg cgttcactga aggcggtcct gcgcaatttg tgaatgcgac 2401 gagcaaagaa gtggttgaat gggcggctaa gttagggctt ccactcgtgt ttagatggga 2461 cgactcaaac gctcaaagaa aagaatacgc cggtttgtac cacgaagttg ctcaggcaca 2521 tggtgtcgat gttagtcagg ttcgacacaa gctgacgctg ctggtcaacc aaaatgtaga 2581 tggtgaagca gcaagggcag aagctcgcgt gtatttggaa gagtttgtcc gtgaatctta 2641 ctcaaatacc gactttgagc aaaaaatggg agagctgttg tcagaaaatg ccatcggtac 2701 ttatgaagaa agtactcagg cagcgcgagt tgcgattgag tgttgtggtg ccgcggacct 2761 attgatgtct tttgagtcga tggaagataa agcgcagcaa agagcggtta tcgatgtggt 2821 aaacgccaac atcgtcaaat accactcgta aaagctttag ctaattaatt ggggacccta 2881 gaggtcccct tttttatttt atcgataccg tcgacctcga gggggggccc gccggggatc 2941 atcgagccga gaacgttatc gaagttggtc atgtgtaatc ccctcgtttg aactttggat 3001 taagcgtaga tacacccttg gacaagccag ttggattcgg agacaagcaa attcagcctt 3061 aaaaagggcg aggcctgcgg tggtggaaca ccgcagggcc tctaaccgct cgacgcgctg 3121 caccaaccag cccgcgaacg gctggcagcc agcgtaaggc gcggctcatc gggcggcgtt 3181 cgccacgatg tcctgcactt cgagccaagc ctcgaacacc tgctggtgtg cacgactcac 3241 ccggttgttg acaccgcgcg cggccgtgcg ggctcggtgg ggcggctgtg tcgcccttgc 3301 cagcgtgagt agcgcgtacc tcacctcgcc caacaggtcg cacacagccg attcgtacgc 3361 cataaagcca ggtgagccca ccagctccgt aagttcgggc gctgtgtggc tcgtacccgc 3421 gcattcaggc ggcagggggt ctaacgggtc taaggcggcg tgtacggccg ccacagcggc 3481 tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt tggtacaggt 3541 ggttgggggt gctcggctgt cgctggtgtt ccaccaccag ggctcgacgg gagagcgggg 3601 gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg acttggagct cgtgtcggac 3661 catacaccgg tgtacgatcg actgccaggc atcaaataaa acgaaaggct cagtcgaaag 3721 actgggcctt tcgttttatg ccatcatggc cgcggtgatc agctagagcc gtgaacgaca 3781 gggcgaacgc cagcccgccg acggcgaggg ttccgaccgc tgcaactccc ggtgcaacct 3841 tgtcccggtc tattctcttc actgcaccag ctccaatctg gtgtgaatgc ccctcgtctg 3901 ttcgcgcagg cggggggctc tattcgtttg tcagcatcga aagtagccag atcagggatg 3961 cgttgcaacc gcgtatgccc aggtcagaag agtcgcacaa gagttgcaga cccctggaaa 4021 gaaaaatggc cagagggcga aaacaccctc tgaccagcgg agcgggcgac gggaatcgaa 4081 cccgcgtagc tagtttggaa gaatgggtgt ctgccgacca catatatggg ccggtcaaga 4141 taggttttta ccccctctcg gctgcatcct ctaagtggaa agaaattgca ggtcgtagaa 4201 gcgcgttgaa gcctgagagt tgcacaggag ttgcaacccg gtagccttgt tcacgacgag 4261 aggagaccta gttggcacgt cgcggatggg gatcgctgaa gactcagcgc agcgggagga 4321 tccaagcctc atacgtcaac ccgcaggacg gtgtgaggta ctacgcgctg cagacctacg 4381 acaacaagat ggacgccgaa gcctggctcg cgggcgagaa gcggctcatc gagatggaga 4441 cctggacccc tccacaggac cgggcgaaga aggcagccgc cagcgccatc acgctggagg 4501 agtacacccg gaagtggctc gtggagcgcg acctcgcaga cggcaccagg gatctgtaca 4561 gcgggcacgc ggagcgccgc atctacccgg tgctaggtga agtggcggtc acagagatga 4621 cgccagctct ggtgcgtgcg tggtgggccg ggatgggtag gaagcacccg actgcccgcc 4681 ggcatgccta caacgtcctc cgggcggtga tgaacacagc ggtcgaggac aagctgatcg 4741 cagagaaccc gtgccggatc gagcagaagg cagccgatga gcgcgacgta gaggcgctga 4801 cgcctgagga gctggacatc gtcgccgctg agatcttcga gcactaccgg atcgcggcat 4861 acatcctggc gtggacgagc ctccggttcg gagagctgat cgagcttcgc cgcaaggaca 4921 tcgtggacga cggcatgacg atgaagctcc gggtgcgccg tggcgcttcc cgcgtgggga 4981 acaagatcgt cgttggcaac gccaagaccg tccggtcgaa gcgtcctgtg acggttccgc 5041 ctcacgtcgc ggagatgatc cgagcgcaca tgaaggaccg tacgaagatg aacaagggcc 5101 ccgaggcatt cctggtgacc acgacgcagg gcaaccggct gtcgaagtcc gcgttcacca 5161 agtcgctgaa gcgtggctac gccaagatcg gtcggccgga actccgcatc cacgacctcc 5221 gcgctgtcgg cgctacgttc gccgctcagg caggtgcgac gaccaaggag ctgatggccc 5281 gtctcggtca cacgactcct aggatggcga tgaagtacca gatggcgtct gaggcccgcg 5341 acgaggctat cgctgaggcg atgtccaagc tggccaagac ctcctgaaac gcaaaaagcc 5401 cccctcccaa ggacactgag tcctaaagag gggggtttct tgtcagtacg cgaagaacca 5461 cgcctggccg cgagcgccag caccgccgct ctgtgcggag acctgggcac cagccccgcc 5521 gccgccagga gcattgccgt tcccgccagc tagcaacaat tggggagcgg gtgtcgcggg 5581 ggttccgtgg ggggttccgt tgcaacgggt cggacaggta aaagtcctgg tagacgctag 5641 ttttctggtt tgggccatgc ctgtctcgtt gcgtgtttcg ttgcgcccgt tttgaatacc 5701 agccagacga gacggggttc tacgaatctt ggtcgatacc aagccatttc cgctgaatat 5761 cggggagctc accgccagaa tcggtggttg tggtgatgta cgtggcgaac tccgttgtag 5821 tgcctgtggt ggcatccgtg gccactctcg ttgcacggtt cgttgtgccg ttacaggccc 5881 cgttgacagc tcaccgaacg tagttaaaac atgctggtca aactaggttt accaacgata 5941 cgagtcagct catctagggc cagttctagg cgttgttcgt tgcgcggttc gttgcgcatg 6001 tttcgtgtgg ttgctagatg gctccgcaac cacacgcttc gaggttgagt gcttccagca 6061 cgggcgcgat ccagaagaac ttcgtcgtgc gactgtcctc gttgatcctt gccgagctgg 6121 gatggaagct cggccgacca ccctggagga gatgatcgag gatgccaggg cctttcacgc 6181 ccgccgctgc tgagcgtccg ccgccgggcc cgcaccgccg tcggccggcc cgctccgggc 6241 tcgcagcagc gggcttcggc gcgggcccgg ggctcccgag cgcgggcggg gctccgggcg 6301 gccgccgggg gccgggggcg gcgccgggcg gcccggggcg tcaggcgccg ggggcggtgt 6361 ccggcggccc ccagaggaac tgcgccagtt cctccggatc ggtgaagccg gagagatcca 6421 gcggggtctc ctcgaacacc tcgaagtcgt gcaggaaggt gaaggcgagc agttcgcggg 6481 cgaagtcctc ggtccgcttc cactgcgccc cgtcgagcag cgcggccagg atctcgcggt 6541 cgccccggaa ggcgttgaga tgcagttgca ccaggctgta gcgggagtct cccgcataga 6601 cgtcggtgaa gtcgacgatc ccggtgacct cggtcgcggc caggtccacg aagatgttgg 6661 tcccgtgcag gtcgccgtgg acgaaccggg gttcgcggcc ggccagcagc gtgtccacgt 6721 ccggcagcca gtcctccagg cggtccagca gccggggcga gaggtagccc cacccgcggt 6781 ggtcctcgac ggtcgccgcg cggcgttccc gcagcagttc cgggaagacc tcggaatggg 6841 gggtgagcac ggtgttcccg gtcagcggca ccctgtgcag ccggccgagc acccggccga 6901 gttcgcgggc cagggcgagc agcgcgttcc ggtcggtcgt gccgtccatc gcggaccgcc 6961 aggtggtgcc ggtcatccgg ctcatcacca ggtagggcca cggccaggct ccggtgccgg 7021 gccgcagctc gccgcggccg aggaggcggg gcaccggcac cggggcgtcc gccaggaccg 7081 cgtacgcctc cgactccgac gcgaggctct ccggaccgca ccagtgctcg ccgaacagct 7141 tgatcaccgg gtcgggctcg ccgaccagta cggggttggt gctctcgccg ggcacccgca 7201 gcaccggcgg caccggcagc ccgagctcct ccagggctcg gcgggccagc ggctcccaga 7261 attcctggtc gttccgcagg ctcgcgtagg aatcatccga atcaatacgg tcgagaagta 7321 acagggattc ttgtgtcaca gcggacctct attcacaggg tacgggccgg cttaattccg 7381 cacggccggt cgcgacacgg cctgtccgca ccgcggtcag gcgttgacga tgacgggctg 7441 gtcggccacg tccggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 7501 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 7561 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 7621 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 7681 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 7741 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 7801 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 7861 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 7921 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 7981 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 8041 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 8101 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 8161 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 8221 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 8281 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 8341 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 8401 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 8461 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat 8521 gcagctagaa ctagt //