质粒名称: | pKW08-Lx |
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出品公司: | 爱迪基因 |
目录编号: | 25012 |
存储实验室: | Brian Robertson |
载体骨架基本信息 |
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载体名称: | pMC1M |
载体出品公司: | -- |
载体抗性: | 潮霉素 |
空载体大小: | 7960 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 细菌表达 |
筛选标记: | -- |
高拷贝/低拷贝: | 低拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | pKW08-Lx |
物种来源: | -- |
插入大小: | 7960 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pKW08-Lx was a gift from Brian Robertson (Addgene plasmid # 25012 ; http://n2t.net/addgene:25012 ; RRID:Addgene_25012) |
参考文献部分: | Improved mycobacterial tetracycline inducible vectors. Williams KJ, Joyce G, Robertson BD. Plasmid. 2010 Apr 29. ():. 10.1016/j.plasmid.2010.04.003 PubMed 20434484 |
买家导航
pKW08Lx载体基本信息
pKW08Lx载体图谱质粒图谱和多克隆位点信息
pKW08Lx载体简介
pKW08Lx载体序列
LOCUS pKW08-Lx 7960 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pKW08-Lx SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7960) AUTHORS Williams KJ, Joyce G, Robertson BD TITLE Improved mycobacterial tetracycline inducible vectors. JOURNAL Plasmid. 2010 Apr 29. ():. PUBMED 20434484 REFERENCE 2 (bases 1 to 7960) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..7960 /organism="synthetic DNA construct" /mol_type="other DNA" terminator 14..41 /label=rrnB T2 terminator /note="transcription terminator T2 from the E. coli rrnB gene" primer_bind complement(2903..2919) /label=KS primer /note="common sequencing primer, one of multiple similar variants" primer_bind complement(2904..2920) /label=pBluescriptKS /note="For pBluescript vector" rep_origin 7125..7713 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 7614..7633 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind 7867..7884 /label=L4440 /note="L4440 vector, forward primer" ORIGIN 1 tgattagcta agcagaaggc catcctgacg gatggccttt ttgcgtttaa tactgtttaa 61 acctctagat cacgattcgc tcgaggtcaa tgacgctgtg cgggagtgag agaatccgtc 121 gatcagtgct gtcaggccga aatcgaattc ccggtcgcgt tcaggaacgc tcgcattgcc 181 gacctctgcg gtttgctcct ccagtacgac accgacggtg tatcggctga ccgcgatgag 241 catgctgatg gctgtctgtt catcgaatcc ctgagacacg agaagcccga cttgctgacc 301 ggaagcctca gctccttcgt cggtgtcgtc gccggatggt ctgcggtggt gctcagcgtg 361 gagacgcgca ccgtcacgga ccgcgtgtag tgcgtggcgg aaacttcttg cgtttcgcaa 421 gagaaatgcg tcccatttct cgtcggactc ggggaaggaa gcgtgatgct ctcggtcaag 481 cacgtcgctc gccagcgctg cgaggagttc ggccttcgtg cggaagtgcc agtagaggcc 541 gggctgctgt acctgtaagt gagccgccag cgcgcgagtg gtgaagccat cgagcccagt 601 ctcgtcgagc acctgccggg ccccgagcaa cacggacgtg cggtcgagac gcttccggtg 661 gtgagtcata gttgcacttt atcatcgata actttatctt agataaagtg actgctcgct 721 actctcatct gactgctcgc tactctcatc gtggaatcct gacaggatcc ggaggaaatg 781 ttatgaaatt tggaaacttc ctcctcactt atcagccacc tgagctatct cagaccgaag 841 tgatgaagcg attggttaat ctgggcaaag cgtctgaagg ttgtggcttc gacaccgttt 901 ggttgctaga gcaccacttc actgaatttg ggttgttagg gaatccttat gttgctgccg 961 cacacctatt aggtgcgaca gaaacgctca acgttggcac tgcagctatc gtattgccga 1021 ctgcccatcc ggttcgacaa gcagaagacg taaacctact ggatcaaatg tcaaaaggac 1081 gattccgttt tggtatttgt cgcggtttgt acgataaaga ttttcgtgtc tttggtacag 1141 acatggataa cagccgagcc ttaatggact gttggtatga cttgatgaaa gaaggcttca 1201 atgaaggcta tatcgcggcg gataacgaac atattaagtt cccgaaaatc caactgaatc 1261 catcggctta cacacaaggt ggtgctcctg tttatgtcgt cgcggagtca gcatcaacga 1321 cagaatgggc tgcagagcgt ggcctaccaa tgattctaag ctggatcatc aacactcacg 1381 agaagaaagc gcagcttgat ctttacaacg aagtcgcgac tgaacatggc tacgatgtga 1441 ctaagattga ccactgtttg tcttacatca cctccgtcga tcatgactca aatagagcca 1501 aagatatttg ccgcaacttc ttgggccatt ggtacgactc atacgtgaat gccaccaaga 1561 tttttgacga ctctgaccaa acaaaaggtt acgacttcaa taaaggtcaa tggcgtgatt 1621 ttgtgttgaa aggccacaaa gacaccaatc gccgaattga ttacagctac gaaatcaacc 1681 cagtagggac gcctgaagag tgtatcgcga ttatccagca agatattgat gcgacgggta 1741 ttgacaatat ttgttgtggt tttgaagcaa acggttctga agaagaaatt atcgcatcta 1801 tgaagctatt ccagtctgat gtgatgccat atctcaaaga aaaacagtaa ttaatatttt 1861 ctaaaaggaa agagacatga aatttggatt attcttcctc aattttatga actcaaagcg 1921 ttcttctgat caagtcatcg aagaaatgtt agataccgca cattacgtag atcagttgaa 1981 gtttgacacg ttggctgttt acgaaaacca tttctcgaac aatggtgtgg ttggtgcccc 2041 actaacagtg gctggttttt tacttggtat gacaaagaac gccaaagtgg cttcgttgaa 2101 tcacgtcatt accacgcatc atccagtacg tgtggcggaa gaagcgtgtc tacttgacca 2161 aatgagtgaa ggccgttttg cctttggctt tagtgattgt gaaaagagtg cagatatgcg 2221 cttctttaat cgaccaacgg attctcagtt tcagttgttc agtgagtgtc acaagatcat 2281 caatgattca ttcactactg ggtactgcca tccaaacaat gatttttata gttttcctaa 2341 aatctccgtt aacccacacg cgttcactga aggcggtcct gcgcaatttg tgaatgcgac 2401 gagcaaagaa gtggttgaat gggcggctaa gttagggctt ccactcgtgt ttagatggga 2461 cgactcaaac gctcaaagaa aagaatacgc cggtttgtac cacgaagttg ctcaggcaca 2521 tggtgtcgat gttagtcagg ttcgacacaa gctgacgctg ctggtcaacc aaaatgtaga 2581 tggtgaagca gcaagggcag aagctcgcgt gtatttggaa gagtttgtcc gtgaatctta 2641 ctcaaatacc gactttgagc aaaaaatggg agagctgttg tcagaaaatg ccatcggtac 2701 ttatgaagaa agtactcagg cagcgcgagt tgcgattgag tgttgtggtg ccgcggacct 2761 attgatgtct tttgagtcga tggaagataa agcgcagcaa agagcggtta tcgatgtggt 2821 aaacgccaac atcgtcaaat accactcgta aaagctttag ctaattaatt ggggacccta 2881 gaggtcccct tttttatttt atcgataccg tcgacctcga gggggggccc gccggggatc 2941 atcgagccga gaacgttatc gaagttggtc atgtgtaatc ccctcgtttg aactttggat 3001 taagcgtaga tacacccttg gacaagccag ttggattcgg agacaagcaa attcagcctt 3061 aaaaagggcg aggcctgcgg tggtggaaca ccgcagggcc tctaaccgct cgacgcgctg 3121 caccaaccag cccgcgaacg gctggcagcc agcgtaaggc gcggctcatc gggcggcgtt 3181 cgccacgatg tcctgcactt cgagccaagc ctcgaacacc tgctggtgtg cacgactcac 3241 ccggttgttg acaccgcgcg cggccgtgcg ggctcggtgg ggcggctgtg tcgcccttgc 3301 cagcgtgagt agcgcgtacc tcacctcgcc caacaggtcg cacacagccg attcgtacgc 3361 cataaagcca ggtgagccca ccagctccgt aagttcgggc gctgtgtggc tcgtacccgc 3421 gcattcaggc ggcagggggt ctaacgggtc taaggcggcg tgtacggccg ccacagcggc 3481 tctcagcggc ccggaaacgt cctcgaaacg acgcatgtgt tcctcctggt tggtacaggt 3541 ggttgggggt gctcggctgt cgctggtgtt ccaccaccag ggctcgacgg gagagcgggg 3601 gagtgtgcag ttgtggggtg gcccctcagc gaaatatctg acttggagct cgtgtcggac 3661 catacaccgg tgattaatcg tggtctacta ccaagcgtga gccacgtcgc cgacgaattt 3721 gagcagctct ggctgccgta ctggccgctg gcaagcgacg atctgctcga ggggatctac 3781 cgccaaagcc gcgcgtcggc cctaggccgc cggtacatcg aggcgaaccc aacagcgctg 3841 gcaaacctgc tggtcgtgga cgtagaccat ccagacgcag cgctccgagc gctcagcgcc 3901 cgggggtccc atccgctgcc caacgcgatc gtgggcaatc gcgccaacgg ccacgcacac 3961 gcagtgtggg cactcaacgc ccctgttcca cgcaccgaat acgcgcggcg taagccgctc 4021 gcatacatgg cggcgtgcgc cgaaggcctt cggcgcgccg tcgacggcga ccgcagttac 4081 tcaggcctca tgaccaaaaa ccccggccac atcgcctggg aaacggaatg gctccactca 4141 gatctctaca cactcagcca catcgaggcc gagctcggcg cgaacatgcc accgccgcgc 4201 tggcgtcagc agaccacgta caaagcggct ccgacgccgc tagggcggaa ttgcgcactg 4261 ttcgattccg tcaggttgtg ggcctatcgt cccgccctca tgcggatcta cctgccgacc 4321 cggaacgtgg acggactcgg ccgcgcgatc tatgccgagt gccacgcgcg aaacgccgaa 4381 ttcccgtgca acgacgtgtg tcccggaccg ctaccggaca gcgaggtccg cgccatcgcc 4441 aacagcattt ggcgttggat cacaaccaag tcgcgcattt gggcggacgg gatcgtggtc 4501 tacgaggcca cactcagtgc gcgccagtcg gccatctcgc ggaagggcgc agcagcgcgc 4561 acggcggcga gcacagttgc gcggcgcgca aagtccgcgt cagccatgga ggcattgcta 4621 tgagcgacgg ctacagcgac ggctacagcg acggctacaa ccggcagccg actgtccgca 4681 aaaagcggcg cgtgaccgcc gccgaaggcg ctcgaatcac cggactatcc gaacgccacg 4741 tcgtccggct cgtggcgcag gaacgcagcg agtggctcgc cgagcaggct gcacgccgcg 4801 aacgcatccg cgcctatcac gacgacgagg gccactcttg gccgcaaacg gccaaacatt 4861 tcgggctgca tctggacacc gttaagcgac tcggctatcg ggcgaggaaa gagcgtgcgg 4921 cagaacagga agcggctcaa aaggcccaca acgaagccga caatccaccg ctgttctaac 4981 gcaattgggg agcgggtgtc gcgggggttc cgtggggggt tccgttgcaa cgggtcggac 5041 aggtaaaagt cctggtagac gctagttttc tggtttgggc catgcctgtc tcgttgcgtg 5101 tttcgttgcg cccgttttga ataccagcca gacgagacgg ggttctacga atcttggtcg 5161 ataccaagcc atttccgctg aatatcgggg agctcaccgc cagaatcggt ggttgtggtg 5221 atgtacgtgg cgaactccgt tgtagtgcct gtggtggcat ccgtggccac tctcgttgca 5281 cggttcgttg tgccgttaca ggccccgttg acagctcacc gaacgtagtt aaaacatgct 5341 ggtcaaacta ggtttaccaa cgatacgagt cagctcatct agggccagtt ctaggcgttg 5401 ttcgttgcgc ggttcgttgc gcatgtttcg tgtggttgct agatggctcc gcaaccacac 5461 gcttcgaggt tgagtgcttc cagcacgggc gcgatccaga agaacttcgt cgtgcgactg 5521 tcctcgttga tccttgccga gctgggatgg aagctcggcc gaccaccctg gaggagatga 5581 tcgaggatgc cagggccttt cacgcccgcc gctgctgagc gtccgccgcc gggcccgcac 5641 cgccgtcggc cggcccgctc cgggctcgca gcagcgggct tcggcgcggg cccggggctc 5701 ccgagcgcgg gcggggctcc gggcggccgc cgggggccgg gggcggcgcc gggcggcccg 5761 gggcgtcagg cgccgggggc ggtgtccggc ggcccccaga ggaactgcgc cagttcctcc 5821 ggatcggtga agccggagag atccagcggg gtctcctcga acacctcgaa gtcgtgcagg 5881 aaggtgaagg cgagcagttc gcgggcgaag tcctcggtcc gcttccactg cgccccgtcg 5941 agcagcgcgg ccaggatctc gcggtcgccc cggaaggcgt tgagatgcag ttgcaccagg 6001 ctgtagcggg agtctcccgc atagacgtcg gtgaagtcga cgatcccggt gacctcggtc 6061 gcggccaggt ccacgaagat gttggtcccg tgcaggtcgc cgtggacgaa ccggggttcg 6121 cggccggcca gcagcgtgtc cacgtccggc agccagtcct ccaggcggtc cagcagccgg 6181 ggcgagaggt agccccaccc gcggtggtcc tcgacggtcg ccgcgcggcg ttcccgcagc 6241 agttccggga agacctcgga atggggggtg agcacggtgt tcccggtcag cggcaccctg 6301 tgcagccggc cgagcacccg gccgagttcg cgggccaggg cgagcagcgc gttccggtcg 6361 gtcgtgccgt ccatcgcgga ccgccaggtg gtgccggtca tccggctcat caccaggtag 6421 ggccacggcc aggctccggt gccgggccgc agctcgccgc ggccgaggag gcggggcacc 6481 ggcaccgggg cgtccgccag gaccgcgtac gcctccgact ccgacgcgag gctctccgga 6541 ccgcaccagt gctcgccgaa cagcttgatc accgggtcgg gctcgccgac cagtacgggg 6601 ttggtgctct cgccgggcac ccgcagcacc ggcggcaccg gcagcccgag ctcctccagg 6661 gctcggcggg ccagcggctc ccagaattcc tggtcgttcc gcaggctcgc gtaggaatca 6721 tccgaatcaa tacggtcgag aagtaacagg gattcttgtg tcacagcgga cctctattca 6781 cagggtacgg gccggcttaa ttccgcacgg ccggtcgcga cacggcctgt ccgcaccgcg 6841 gtcaggcgtt gacgatgacg ggctggtcgg ccacgtccgg ggagtcaggc aactatggat 6901 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca 6961 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg 7021 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 7081 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 7141 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 7201 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 7261 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 7321 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 7381 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 7441 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 7501 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 7561 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 7621 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 7681 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 7741 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct 7801 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc 7861 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc 7921 cccgcgcgtt ggccgattca ttaatgcagc tagaactagt //
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