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pEX-C-GST载体质粒基本信息
pEX-C-GST质粒图谱载体图谱和pEX-C-GST载体序列质粒序列多克隆位点信息
pEX-C-GST质粒载体简介
pEX-C-GST质粒序列载体序列
LOCUS pEX-C-GST 5253 bp DNA circular SYN 07-MAR-2024
DEFINITION PrecisionShuttle(TM) bacterial vector for inducible expression of a
protein with a cleavable C-terminal GST tag.
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5253)
AUTHORS OriGene
TITLE Direct Submission
JOURNAL Exported Nov 12, 2024 from SnapGene 6.0.2 to Vector NTI(R) format
https://www.snapgene.com
COMMENT Inserts are typically cloned between the BseRI and MluI sites. The
BseRI site is compatible with SgfI sites from other
PrecisionShuttle(TM) vectors.
COMMENT VNTDATE|690076800|
COMMENT VNTDBDATE|1051228800|
COMMENT VNTNAME|pEX-C-GST|
COMMENT VNTAUTHORNAME|OriGene|
FEATURES Location/Qualifiers
promoter 216..234
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 235..259
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 279..284
/label=RBS
/note="ribosome binding site"
misc_feature 286..361
/label=MCS
/note="multiple cloning site"
CDS 362..382
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV Site
CDS 383..1036
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
CDS 1058..1078
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV Site
terminator 1169..1215
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter 1461..1538
/gene="lacI"
/label=lacI promoter
CDS 1539..2621
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
rep_origin complement(3072..3660)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(3831..4691)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(4692..4796)
/gene="bla"
/label=AmpR promoter
rep_origin join(4823..5253,1..25)
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 aacaaaatat taacgcttac aatttccatt cgccattcag gctgcgcaac tgttgggaag
61 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
121 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttaaaac gacggccagt
181 gccgaattct gctggacttt ccaaaatgtc gagcttaata cgactcacta taggggaatt
241 gtgagcggat aacaattccc cttttgttta actttaagaa ggagatatcg atcgctctcc
301 tcggcgcgcc agatctcaag cttaactagc tagcggaccg acgcgtacgc ggccgctcga
361 ggaaaacctg tattttcagg gcatgtcccc tatactaggt tattggaaaa ttaagggcct
421 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
481 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
541 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
601 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
661 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
721 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
781 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
841 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
901 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
961 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
1021 cgaccatcct ccaaaatcgg atatcatccc aacgaccgaa aacctgtatt ttcagggcgc
1081 gatcgccggc gcgccagatc tcaagcttaa ctaggtttaa acggccggcc acatgactga
1141 ctgagccacg ataaccgctg agcaataact agcataaccc cttggggcct ctaaacgggt
1201 cttgaggggt tttttcccac cagccttgtc ctaatccgcg gcggttcctg gccttttgct
1261 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
1321 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
1381 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta
1441 tttcacaccg cataaattcc gacaccatcg aatggcgcaa aacctttcgc ggtatggcat
1501 gatagcgccc ggaagagagt caattcaggg tggtgaatgt gaaaccagta acgttatacg
1561 atgtcgcaga gtatgccggt gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca
1621 gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc gatggcggag ctgaattaca
1681 ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc gttgctgatt ggcgttgcca
1741 cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg
1801 atcaactggg tgccagcgtg gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta
1861 aagcggcggt gcacaatctt ctcgcgcaac gagtcagtgg gctgatcatt aactatccgc
1921 tggatgacca ggatgccatt gctgtggaag ctgcctgcac taatgttccg gcgttatttc
1981 ttgatgtctc tgaccagaca cccatcaaca gtattatttt ctcccatgaa gacggtacgc
2041 gactgggcgt ggagcatctg gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc
2101 cattaagttc tgtctcggcg cgtctgcgtc tggctggctg gcataaatat ctcactcgca
2161 atcaaattca gccgatagcg gaacgggaag gcgactggag tgccatgtcc ggttttcaac
2221 aaaccatgca aatgctgaat gagggcatcg ttcccactgc gatgctggtt gccaacgatc
2281 agatggcgct gggcgcaatg cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata
2341 tctcggtagt gggatacgac gataccgaag acagctcatg ttatatcccg ccgttaacca
2401 ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct
2461 ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa
2521 ccaccctggc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
2581 agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgta
2641 agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg
2701 tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgga
2761 ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa
2821 tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga
2881 tggatccgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
2941 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
3001 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
3061 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
3121 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
3181 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
3241 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
3301 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
3361 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
3421 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
3481 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
3541 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
3601 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
3661 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
3721 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
3781 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
3841 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
3901 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
3961 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
4021 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
4081 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
4141 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
4201 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
4261 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
4321 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
4381 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
4441 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
4501 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
4561 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
4621 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
4681 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
4741 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
4801 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
4861 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
4921 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
4981 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
5041 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
5101 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
5161 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
5221 gagctgattt aacaaaaatt taacgcgaat ttt
//
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