| 质粒名称: | pHCMV-10A1 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 15805 |
| 存储实验室: | Miguel Sena-Esteves |
|
载体骨架基本信息 |
|
| 载体名称: | pHMCV |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 4839 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | 10A1 Murine leukemia virus envelope mRNA |
| 物种来源: | Moloney murine leukemia virus 10A1 strain |
| 插入大小: | 1938 bp |
| 基因突变: | -- |
| GenBank编号 | M33470 |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHCMV-10A1 was a gift from Miguel Sena-Esteves (Addgene plasmid # 15805 ; http://n2t.net/addgene:15805 ; RRID:Addgene_15805) |
| 参考文献部分: | Optimized large-scale production of high titer lentivirus vector pseudotypes. Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW. J Virol Methods. 2004 Dec 15. 122(2):131-9. 10.1016/j.jviromet.2004.08.017 PubMed 15542136 |
买家导航
pHCMV10A1载体基本信息
pHCMV10A1载体图谱质粒图谱和多克隆位点信息
pHCMV10A1载体简介
pHCMV10A1载体序列
LOCUS pHCMV-10A1 6775 bp ds-DNA circular SYN 02-MAY-2019
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pHCMV-10A1
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6775)
AUTHORS Sena-Esteves M, Tebbets JC, Steffens S, Crombleholme T, Flake AW
TITLE Optimized large-scale production of high titer lentivirus vector
pseudotypes.
JOURNAL J Virol Methods. 2004 Dec 15. 122(2):131-9.
PUBMED 15542136
REFERENCE 2 (bases 1 to 6775)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 2, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6775
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..204
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 155..175
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 201..225
/label=LNCX
/note="Human CMV promoter, forward primer"
intron 316..888
/label=-beta-globin intron
/note="intron from rabbit beta-globin gene"
RBS 1933..1941
/label=Shine-Dalgarno sequence
/note="full consensus sequence for ribosome-binding sites
upstream of start codons in E. coli; complementary to a
region in the 3' end of the 16S rRNA (Chen et al., 1994)"
primer_bind complement(3003..3022)
/label=Bglob-pA-R
/note="Rabbit beta-globin polyA region, reverse primer"
polyA_site 3068..3123
/label=-beta-globin poly(A) signal
/note="rabbit beta-globin polyadenylation signal"
primer_bind complement(3122..3141)
/label=rbglobpA-R
/note="Rabbit beta-globin polyA, reverse primer. Also
called rb-glob-pA-term-R"
primer_bind complement(3482..3498)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3482..3498)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3495..3517)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3506..3522
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3530..3560)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3575..3596
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3713..3730)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3884..4472)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3964..3983)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4643..5503)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 5266..5285
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5504..5608)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5634..6089)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5771..5792)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 5983..6002
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6216..6238
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6230..6247
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6231..6247
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 6257..6276
/label=T7
/note="T7 promoter, forward primer"
promoter 6257..6275
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
enhancer 6396..6775
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
61 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
121 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
181 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
241 ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg gtcgaccgat
301 cctgagaact tcagggtgag tttggggacc cttgattgtt ctttcttttt cgctattgta
361 aaattcatgt tatatggagg gggcaaagtt ttcagggtgt tgtttagaat gggaagatgt
421 cccttgtatc accatggacc ctcatgataa ttttgtttct ttcactttct actctgttga
481 caaccattgt ctcctcttat tttcttttca ttttctgtaa ctttttcgtt aaactttagc
541 ttgcatttgt aacgaatttt taaattcact tttgtttatt tgtcagattg taagtacttt
601 ctctaatcac ttttttttca aggcaatcag ggtatattat attgtacttc agcacagttt
661 tagagaacaa ttgttataat taaatgataa ggtagaatat ttctgcatat aaattctggc
721 tggcgtggaa atattcttat tggtagaaac aactacaccc tggtcatcat cctgcctttc
781 tctttatggt tacaatgata tacactgttt gagatgagga taaaatactc tgagtccaaa
841 ccgggcccct ctgctaacca tgttcatgcc ttcttctctt tcctacagct cctgggcaac
901 gtgctggttg ttgtgctgtc tcatcatttt ggcaaagaat tcctcgacgg atctgggccg
961 caccatggcg cgttcaacgc tctcaaaacc ccttaaagat aagattaacc cgtggaagtc
1021 cttaatggtc atgggggtcc tattaagagt agggatggca gagagccccc atcaggtctt
1081 taatgtaacc tggagagtca ccaacctgat gaccgggcgt accgccaatg ccacctccct
1141 tttaggaact gtacaagatg ccttcccaag attatatttt gatctatgtg acctggtcgg
1201 agaagagtgg gacccttcag accaggaacc atatgtcggg tatggctgca aataccccgg
1261 agggagaaag cggacccgga cttttgactt ttacgtgtgc cctgggcata ccgtaaaatc
1321 ggggtgtggg gggccaagag aaggctactg tggtgaatgg ggttgtgaaa ccaccggaca
1381 ggcttactgg aagcccacat catcatggga cctaatctcc cttaagcgcg gtaacacccc
1441 ctgggacacg ggatgctcca aaatggcttg tggcccctgc tatgacctct ccaaggtatc
1501 caattccttc caaggggcta ctcgaggggg cagatgcaac cctctagtcc tagaattcac
1561 tgatgcagga aaaaaggcta attgggacgg gcccaaatcg tggggactga gactgtaccg
1621 gacaggaaca gatcctatta ccatgttctc cctgacccgc caggtcctca atatagggcc
1681 ccgcatcccc attgggccta atcccgtgat cactggtcaa ctacccccct cccgacccgt
1741 gcagatcagg ctccccaggc ctcctcagcc tcctcctaca ggcgcagcct ctatagtccc
1801 tgagactgcc ccaccttctc aacaacctgg gacgggagac aggctgctaa acctggtaga
1861 aggagcctat cgggcgctta acctcaccaa tcccgacaag acccaagaat gttggctgtg
1921 cttagtgtcg ggacctcctt attacgaagg agtagcggtc gtgggcactt ataccaatca
1981 ttctaccgcc ccggccagct gtacggccac ttcccaacat aagcttaccc tatctgaagt
2041 gacaggacag ggcctatgca tgggagcagt acctaaaact caccaggcct tatgtaacac
2101 cacccaaagt gccggctcag gatcctacta ccttgcagca cccgctggaa caatgtgggc
2161 ttgtagcact ggattgactc cctgcttgtc caccacgatg ctcaatctaa ccacagacta
2221 ttgtgtatta gttgagctct ggcccagaat aatttaccac tcccccgatt atatgtatgg
2281 tcagcttgaa cagcgtacca aatataagag ggagccagta tcgttgaccc tggcccttct
2341 gctaggagga ttaaccatgg gagggattgc agctggaata gggacgggga ccactgccct
2401 aatcaaaacc cagcagtttg agcagcttca cgccgctatc cagacagacc tcaacgaagt
2461 cgaaaaatca attaccaacc tagaaaagtc actgacctcg ttgtctgaag tagtcctaca
2521 gaaccgaaga ggcctagatt tgctcttcct aaaagaggga ggtctctgcg cagccctaaa
2581 agaagaatgt tgtttttatg cagaccacac gggactagtg agagacagca tggccaaact
2641 aagggaaagg cttaatcaga gacaaaaact atttgagtca ggccaaggtt ggttcgaagg
2701 gctgtttaat agatccccct ggtttaccac cttaatctcc accatcatgg gacctctaat
2761 agtactctta ctgatcttac tctttggacc ctgcattctc aatcgattag tccaatttgt
2821 taaagacagg atatcagtgg tccaggctct agttttgact caacaatatc accagctgaa
2881 gcccatagag tacgagccat gataaccaga tccgtcgagg aattcactcc tcaggtgcag
2941 gctgcctatc agaaggtggt ggctggtgtg gccaatgccc tggctcacaa ataccactga
3001 gatctttttc cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact
3061 tctggctaat aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc
3121 tcactcggaa ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt
3181 tagagtttgg caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga
3241 ggtcatcagt atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg
3301 acttgaggtt agattttttt ttatattttg ttttgtgtta tttttttctt taacatccct
3361 aaaattttcc ttacatgttt tactagccag atttttcctc ctctcctgac tactcccagt
3421 catagctgtc cctcttctct tatggagatc cctcgacgga tcggccgcaa ttcgtaatca
3481 tgtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
3541 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
3601 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
3661 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
3721 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3781 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3841 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3901 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3961 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
4021 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
4081 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
4141 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
4201 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
4261 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
4321 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
4381 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
4441 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
4501 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
4561 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
4621 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
4681 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
4741 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
4801 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
4861 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
4921 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
4981 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
5041 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
5101 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
5161 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
5221 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
5281 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
5341 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
5401 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
5461 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
5521 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
5581 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctaaattgt
5641 aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct cattttttaa
5701 ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt
5761 gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa
5821 agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac cctaatcaag
5881 ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga gcccccgatt
5941 tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg
6001 agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc
6061 cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc gcaactgttg
6121 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
6181 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
6241 ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc accgcggtgg
6301 cggccgctct agagagcttg gcccattgca tacgttgtat ccatatcata atatgtacat
6361 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta
6421 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
6481 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
6541 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
6601 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
6661 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
6721 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatg
//
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