| 质粒名称: | Lenti-AsCpf1-Blast |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 84750 |
| 存储实验室: | Hyongbum Kim |
|
载体骨架基本信息 |
|
| 载体名称: | lentiCas9-Blast(Addgene#:52962) |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8700 bp |
| 完整质粒大小: | 12670 bp |
| 载体类型: | 哺乳动物表达，慢病毒，CRISPR |
| 筛选标记: | 杀稻瘟菌素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | AsCpf1,Cpf1 |
| 物种来源: | Acidaminococcus_sp_BV3L6 |
| 插入大小: | 3970 bp |
| 启动子 | EFS-NS |
| 标签/融合蛋白: | NLS-3xHA（插接件上的C端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | pLLU-F (5'-gggacagcagagatccagtt-3') |
| 3' 测序引物 | blast-R (5'-gctctttcaatgagggtgga-3') |
|
文献引用方法 |
|
| 材料和方法部分: | Lenti-AsCpf1-Blast was a gift from Hyongbum Kim (Addgene plasmid # 84750 ; http://n2t.net/addgene:84750 ; RRID:Addgene_84750) |
| 参考文献部分: | In vivo high-throughput profiling of CRISPR-Cpf1 activity. Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E, Koh HC, Nam JW, Kim H. Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104. 10.1038/nmeth.4104 PubMed 27992409 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Lenti-AsCpf1-Blast 12670 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Expresses human codon-optimized AsCpf1 protein and blasticidin
resistance from EFS promoter. Lentiviral backbone..
ACCESSION .
VERSION .
KEYWORDS Lenti-AsCpf1-Blast
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12670)
AUTHORS Kim HK, Song M, Lee J, Menon AV, Jung S, Kang YM, Choi JW, Woo E,
Koh HC, Nam JW, Kim H
TITLE In vivo high-throughput profiling of CRISPR-Cpf1 activity.
JOURNAL Nat Methods. 2016 Dec 19. doi: 10.1038/nmeth.4104.
PUBMED 27992409
REFERENCE 2 (bases 1 to 12670)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12670
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 2650..2861
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
CDS 2903..6823
/codon_start=1
/product="CRISPR-associated protein from Acidaminococcus
sp. BV3L6 (Zetsche et al., 2015)"
/label=AsCpf1
/translation="MTQFEGFTNLYQVSKTLRFELIPQGKTLKHIQEQGFIEEDKARND
HYKELKPIIDRIYKTYADQCLQLVQLDWENLSAAIDSYRKEKTEETRNALIEEQATYRN
AIHDYFIGRTDNLTDAINKRHAEIYKGLFKAELFNGKVLKQLGTVTTTEHENALLRSFD
KFTTYFSGFYENRKNVFSAEDISTAIPHRIVQDNFPKFKENCHIFTRLITAVPSLREHF
ENVKKAIGIFVSTSIEEVFSFPFYNQLLTQTQIDLYNQLLGGISREAGTEKIKGLNEVL
NLAIQKNDETAHIIASLPHRFIPLFKQILSDRNTLSFILEEFKSDEEVIQSFCKYKTLL
RNENVLETAEALFNELNSIDLTHIFISHKKLETISSALCDHWDTLRNALYERRISELTG
KITKSAKEKVQRSLKHEDINLQEIISAAGKELSEAFKQKTSEILSHAHAALDQPLPTTL
KKQEEKEILKSQLDSLLGLYHLLDWFAVDESNEVDPEFSARLTGIKLEMEPSLSFYNKA
RNYATKKPYSVEKFKLNFQMPTLASGWDVNKEKNNGAILFVKNGLYYLGIMPKQKGRYK
ALSFEPTEKTSEGFDKMYYDYFPDAAKMIPKCSTQLKAVTAHFQTHTTPILLSNNFIEP
LEITKEIYDLNNPEKEPKKFQTAYAKKTGDQKGYREALCKWIDFTRDFLSKYTKTTSID
LSSLRPSSQYKDLGEYYAELNPLLYHISFQRIAEKEIMDAVETGKLYLFQIYNKDFAKG
HHGKPNLHTLYWTGLFSPENLAKTSIKLNGQAELFYRPKSRMKRMAHRLGEKMLNKKLK
DQKTPIPDTLYQELYDYVNHRLSHDLSDEARALLPNVITKEVSHEIIKDRRFTSDKFFF
HVPITLNYQAANSPSKFNQRVNAYLKEHPETPIIGIDRGERNLIYITVIDSTGKILEQR
SLNTIQQFDYQKKLDNREKERVAARQAWSVVGTIKDLKQGYLSQVIHEIVDLMIHYQAV
VVLENLNFGFKSKRTGIAEKAVYQQFEKMLIDKLNCLVLKDYPAEKVGGVLNPYQLTDQ
FTSFAKMGTQSGFLFYVPAPYTSKIDPLTGFVDPFVWKTIKNHESRKHFLEGFDFLHYD
VKTGDFILHFKMNRNLSFQRGLPGFMPAWDIVFEKNETQFDAKGTPFIAGKRIVPVIEN
HRFTGRYRDLYPANELIALLEEKGIVFRDGSNILPKLLENDDSHAIDTMVALIRSVLQM
RNSNAATGEDYINSPVRDLNGVCFDSRFQNPEWPMDADANGAYHIALKGQLLLNHLKES
KDLKLQNGISNQDWLAYIQELRN"
regulatory 6543..6552
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6824..6871
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS 6881..6937
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 6938..7336
/codon_start=1
/gene="Aspergillus terreus BSD"
/product="blasticidin S deaminase"
/label=BSD
/note="confers resistance to blasticidin"
/translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
KAIVKDSDGQPTAVGIRELLPSGYVWEG"
misc_feature 7361..7949
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(7414..7434)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(7832..7843)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(7952..7968)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7953..7969)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 8474..8654
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(8680..8697)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 8686..8910
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 8956..9384
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(9043..9062)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 9253..9274
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(9393..9413)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 9398..9728
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 9580..9714
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 9641..9660
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 9776..9823
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 9842..10216
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 10346..10467
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(10383..10402)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 10437..10456
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(10516..10532)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(10516..10532)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(10529..10551)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 10540..10556
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(10564..10594)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 10609..10630
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(10747..10764)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10918..11506)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10998..11017)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11677..12537)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 12300..12319
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12538..12642)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttaatta gctagctagg tcttgaaagg agtgggaatt ggctccggtg
2641 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg
2701 gcaattgatc cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt
2761 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg
2821 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggaccggttc tagcgtttaa
2881 acttaagctt ggtaccgcca ccatgacaca gttcgagggc tttaccaacc tgtatcaggt
2941 gagcaagaca ctgcggtttg agctgatccc acagggcaag accctgaagc acatccagga
3001 gcagggcttc atcgaggagg acaaggcccg caatgatcac tacaaggagc tgaagcccat
3061 catcgatcgg atctacaaga cctatgccga ccagtgcctg cagctggtgc agctggattg
3121 ggagaacctg agcgccgcca tcgactccta tagaaaggag aaaaccgagg agacaaggaa
3181 cgccctgatc gaggagcagg ccacatatcg caatgccatc cacgactact tcatcggccg
3241 gacagacaac ctgaccgatg ccatcaataa gagacacgcc gagatctaca agggcctgtt
3301 caaggccgag ctgtttaatg gcaaggtgct gaagcagctg ggcaccgtga ccacaaccga
3361 gcacgagaac gccctgctgc ggagcttcga caagtttaca acctacttct ccggctttta
3421 tgagaacagg aagaacgtgt tcagcgccga ggatatcagc acagccatcc cacaccgcat
3481 cgtgcaggac aacttcccca agtttaagga gaattgtcac atcttcacac gcctgatcac
3541 cgccgtgccc agcctgcggg agcactttga gaacgtgaag aaggccatcg gcatcttcgt
3601 gagcacctcc atcgaggagg tgttttcctt ccctttttat aaccagctgc tgacacagac
3661 ccagatcgac ctgtataacc agctgctggg aggaatctct cgggaggcag gcaccgagaa
3721 gatcaagggc ctgaacgagg tgctgaatct ggccatccag aagaatgatg agacagccca
3781 catcatcgcc tccctgccac acagattcat ccccctgttt aagcagatcc tgtccgatag
3841 gaacaccctg tctttcatcc tggaggagtt taagagcgac gaggaagtga tccagtcctt
3901 ctgcaagtac aagacactgc tgagaaacga gaacgtgctg gagacagccg aggccctgtt
3961 taacgagctg aacagcatcg acctgacaca catcttcatc agccacaaga agctggagac
4021 aatcagcagc gccctgtgcg accactggga tacactgagg aatgccctgt atgagcggag
4081 aatctccgag ctgacaggca agatcaccaa gtctgccaag gagaaggtgc agcgcagcct
4141 gaagcacgag gatatcaacc tgcaggagat catctctgcc gcaggcaagg agctgagcga
4201 ggccttcaag cagaaaacca gcgagatcct gtcccacgca cacgccgccc tggatcagcc
4261 actgcctaca accctgaaga agcaggagga gaaggagatc ctgaagtctc agctggacag
4321 cctgctgggc ctgtaccacc tgctggactg gtttgccgtg gatgagtcca acgaggtgga
4381 ccccgagttc tctgcccggc tgaccggcat caagctggag atggagcctt ctctgagctt
4441 ctacaacaag gccagaaatt atgccaccaa gaagccctac tccgtggaga agttcaagct
4501 gaactttcag atgcctacac tggcctctgg ctgggacgtg aataaggaga agaacaatgg
4561 cgccatcctg tttgtgaaga acggcctgta ctatctgggc atcatgccaa agcagaaggg
4621 caggtataag gccctgagct tcgagcccac agagaaaacc agcgagggct ttgataagat
4681 gtactatgac tacttccctg atgccgccaa gatgatccca aagtgcagca cccagctgaa
4741 ggccgtgaca gcccactttc agacccacac aacccccatc ctgctgtcca acaatttcat
4801 cgagcctctg gagatcacaa aggagatcta cgacctgaac aatcctgaga aggagccaaa
4861 gaagtttcag acagcctacg ccaagaaaac cggcgaccag aagggctaca gagaggccct
4921 gtgcaagtgg atcgacttca caagggattt tctgtccaag tataccaaga caacctctat
4981 cgatctgtct agcctgcggc catcctctca gtataaggac ctgggcgagt actatgccga
5041 gctgaatccc ctgctgtacc acatcagctt ccagagaatc gccgagaagg agatcatgga
5101 tgccgtggag acaggcaagc tgtacctgtt ccagatctat aacaaggact ttgccaaggg
5161 ccaccacggc aagcctaatc tgcacacact gtattggacc ggcctgtttt ctccagagaa
5221 cctggccaag acaagcatca agctgaatgg ccaggccgag ctgttctacc gccctaagtc
5281 caggatgaag aggatggcac accggctggg agagaagatg ctgaacaaga agctgaagga
5341 tcagaaaacc ccaatccccg acaccctgta ccaggagctg tacgactatg tgaatcacag
5401 actgtcccac gacctgtctg atgaggccag ggccctgctg cccaacgtga tcaccaagga
5461 ggtgtctcac gagatcatca aggataggcg ctttaccagc gacaagttct ttttccacgt
5521 gcctatcaca ctgaactatc aggccgccaa ttccccatct aagttcaacc agagggtgaa
5581 tgcctacctg aaggagcacc ccgagacacc tatcatcggc atcgatcggg gcgagagaaa
5641 cctgatctat atcacagtga tcgactccac cggcaagatc ctggagcagc ggagcctgaa
5701 caccatccag cagtttgatt accagaagaa gctggacaac agggagaagg agagggtggc
5761 agcaaggcag gcctggtctg tggtgggcac aatcaaggat ctgaagcagg gctatctgag
5821 ccaggtcatc cacgagatcg tggacctgat gatccactac caggccgtgg tggtgctgga
5881 gaacctgaat ttcggcttta agagcaagag gaccggcatc gccgagaagg ccgtgtacca
5941 gcagttcgag aagatgctga tcgataagct gaattgcctg gtgctgaagg actatccagc
6001 agagaaagtg ggaggcgtgc tgaacccata ccagctgaca gaccagttca cctcctttgc
6061 caagatgggc acccagtctg gcttcctgtt ttacgtgcct gccccatata catctaagat
6121 cgatcccctg accggcttcg tggacccctt cgtgtggaaa accatcaaga atcacgagag
6181 ccgcaagcac ttcctggagg gcttcgactt tctgcactac gacgtgaaaa ccggcgactt
6241 catcctgcac tttaagatga acagaaatct gtccttccag aggggcctgc ccggctttat
6301 gcctgcatgg gatatcgtgt tcgagaagaa cgagacacag tttgacgcca agggcacccc
6361 tttcatcgcc ggcaagagaa tcgtgccagt gatcgagaat cacagattca ccggcagata
6421 ccgggacctg tatcctgcca acgagctgat cgccctgctg gaggagaagg gcatcgtgtt
6481 cagggatggc tccaacatcc tgccaaagct gctggagaat gacgattctc acgccatcga
6541 caccatggtg gccctgatcc gcagcgtgct gcagatgcgg aactccaatg ccgccacagg
6601 cgaggactat atcaacagcc ccgtgcgcga tctgaatggc gtgtgcttcg actcccggtt
6661 tcagaaccca gagtggccca tggacgccga tgccaatggc gcctaccaca tcgccctgaa
6721 gggccagctg ctgctgaatc acctgaagga gagcaaggat ctgaagctgc agaacggcat
6781 ctccaatcag gactggctgg cctacatcca ggagctgcgc aacaaaaggc cggcggccac
6841 gaaaaaggcc ggccaggcaa aaaagaaaaa gggatccggc gcaacaaact tctctctgct
6901 gaaacaagcc ggagatgtcg aagagaatcc tggaccgatg gccaagcctt tgtctcaaga
6961 agaatccacc ctcattgaaa gagcaacggc tacaatcaac agcatcccca tctctgaaga
7021 ctacagcgtc gccagcgcag ctctctctag cgacggccgc atcttcactg gtgtcaatgt
7081 atatcatttt actgggggac cttgtgcaga actcgtggtg ctgggcactg ctgctgctgc
7141 ggcagctggc aacctgactt gtatcgtcgc gatcggaaat gagaacaggg gcatcttgag
7201 cccctgcgga cggtgccgac aggtgcttct cgatctgcat cctgggatca aagccatagt
7261 gaaggacagt gatggacagc cgacggcagt tgggattcgt gaattgctgc cctctggtta
7321 tgtgtgggag ggctaagaat tcgatatcaa gcttatcggt aatcaacctc tggattacaa
7381 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
7441 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
7501 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
7561 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
7621 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
7681 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
7741 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
7801 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
7861 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
7921 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgag acctagaaaa
7981 acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg cctggctaga
8041 agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt taagaccaat
8101 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
8161 gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg
8221 ctacttccct gattggcaga actacacacc agggccaggg atcagatatc cactgacctt
8281 tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg
8341 agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
8401 agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca
8461 tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
8521 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
8581 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
8641 gaaaatctct agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt
8701 gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc
8761 ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt
8821 ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca
8881 ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct
8941 ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta
9001 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc
9061 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt
9121 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg
9181 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca
9241 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct
9301 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga
9361 tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa
9421 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
9481 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
9541 ttagtcagca accatagtcc cgcccctaac tccgcccagt cccgccccta actccgccca
9601 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
9661 ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
9721 tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcag cacgtgttga
9781 caattaatca tcggcatagt atatcggcat agtataatac gacaaggtga ggaactaaac
9841 catggccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt
9901 cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg acttcgccgg
9961 tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga
10021 caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga
10081 ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca
10141 gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc
10201 cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct tctatgaaag
10261 gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc gcggggatct
10321 catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg gttacaaata
10381 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
10441 tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct ctagctagag
10501 cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc
10561 acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta
10621 actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca
10681 gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc
10741 cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
10801 tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
10861 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
10921 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
10981 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
11041 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
11101 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
11161 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
11221 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
11281 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
11341 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
11401 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
11461 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
11521 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
11581 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
11641 aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc
11701 acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta
11761 gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga
11821 cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg
11881 cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc
11941 tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat
12001 cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag
12061 gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat
12121 cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa
12181 ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa
12241 gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga
12301 taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg
12361 gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc
12421 acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg
12481 aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact
12541 cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat
12601 atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt
12661 gccacctgac
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pUC18R6KT-mini-Tn7T-Km
- 下一篇：pET28a_T7-ARG1
