| 质粒名称: | Matrix-roGFP |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 49437 |
| 存储实验室: | Paul Schumacker |
|
载体骨架基本信息 |
|
| 载体名称: | VQ Ad5CMV K-NpA |
| 载体出品公司: | ViraQuest Inc. |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 6217 bp |
| 完整质粒大小: | 6970 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Matrix-roGFP2 |
| 物种来源: | -- |
| 插入大小: | 780 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | AgeI(未破坏) |
| 3' 克隆位点: | NotI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Matrix-roGFP was a gift from Paul Schumacker (Addgene plasmid # 49437 ; http://n2t.net/addgene:49437 ; RRID:Addgene_49437) |
| 参考文献部分: | Hypoxia triggers subcellular compartmental redox signaling in vascular smooth muscle cells. Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D, Schumacker PT. Circ Res. 2010 Feb 19;106(3):526-35. doi: 10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17. 10.1161/CIRCRESAHA.109.206334 PubMed 20019331 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS Matrix-roGFP 6929 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Expresses thiol redox-sensitive ratiometric sensor roGFP in the
mitochondrial matrix of mammalian cells.
ACCESSION .
VERSION .
KEYWORDS Matrix-roGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6929)
AUTHORS Waypa GB, Marks JD, Guzy R, Mungai PT, Schriewer J, Dokic D,
Schumacker PT
TITLE Hypoxia triggers subcellular compartmental redox signaling in
vascular smooth muscle cells.
JOURNAL Circ Res. 2010 Feb 19;106(3):526-35. doi:
10.1161/CIRCRESAHA.109.206334. Epub 2009 Dec 17.
PUBMED 20019331
REFERENCE 2 (bases 1 to 6929)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6929
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 3027..3049
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
polyA_signal 3192..3326
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3229..3248)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3283..3302
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
CDS complement(3367..4086)
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(3412..3433)
/label=EGFP-C
/note="EGFP, forward primer"
primer_bind 3761..3780
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 4020..4041
/label=EGFP-N
/note="EGFP, reverse primer"
promoter complement(4167..4370)
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind complement(4196..4216)
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
enhancer 4371..4674
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
misc_signal 4703..4853
/label=Ad5 Psi
/note="packaging signal for adenovirus serotype 5"
repeat_region 4941..5043
/label=ITR
/note="inverted terminal repeat of human adenovirus
serotype 5"
primer_bind complement(5071..5089)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5157..5261
/gene="bla"
/label=AmpR promoter
CDS 5262..6122
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(5480..5499)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 6293..6881
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6782..6801
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
61 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
121 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
181 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
241 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
301 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
361 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
421 aaggctctca agggcatcgg tcgaggacag gcctctcaag tctgtatacg gggacacgga
481 cagccttttc gtcaccgagc gtggacaccg gctcatggaa accagaggta agaaacgcat
541 caaaaagcat gggggaaacc tggtttttga ccccgaacgg ccagagctca cctggctcgt
601 ggaatgcgag accgtctgcg gggcctgcgg cgcggatgcc tactccccgg aatcggtatt
661 tctcgcgccc aagctctacg ccctcaaaag tctgcactgc ccctcgtgcg gcgcctcctc
721 caagggcaag ctgcgcgcca agggccacgc cgcggagggg ctggactatg acaccatggt
781 caaatgctac ctggccgacg cgcagggcga agaccggcag cgcttcagca ccagcaggac
841 cagcctcaag cgcaccctgg ccagcgcgca gcccggagcg caccccttca ccgtgaccca
901 gactacgctg acgaggaccc tgcgcccgtg gaaagacatg accctggccc gtctggacga
961 gcaccgacta ctgccgtaca gcgaaagccg ccccaacccg cgaaacgagg agatatgctg
1021 gatcgagatg ccgtagagca ggtgaccgag ctgtgggacc gcctggaact gcttggtcaa
1081 acgctcaaaa gcatgcctac ggcggacggt ctcaaaccgt tgaaaaactt tgcttccttg
1141 caagaactgc tatcgctggg cggcgagcgc cttctggcgg atttggtcag ggaaaacatg
1201 cgagtcaggg acatgcttaa cgaagtggcc cccctgctca gggatgacgg cagctgcagc
1261 tctcttaact accagttgca gccggtaata ggtgtgattt acgggcccac cggctgcggt
1321 aagtcgcagc tgctcaggaa cctgctttct tcccagctga tctcccctac cccggaaacc
1381 gttttcttca tcgccccgca ggtagacatg atccccccat ctgaactcaa agcgtgggaa
1441 atgcaaatct gtgagggtaa ctacgcccct gggccggatg gaaccattat accgcagtct
1501 ggcaccctcc gcccgcgctt tgtaaaaatg gcctatgacg atctcatcct ggaacacaac
1561 tatgacgtta gtgatcccag aaatatcttc gcccaggccg ccgcccgtgg gcccattgcc
1621 atcattatgg acgaatgcat ggaaaatctt ggaggtcaca agggcgtctc caagttcttc
1681 cacgcatttc cttctaagct acatgacaaa tttcccaagt gcaccggata cactgtgctg
1741 gtggttctgc acaacatgaa tccccggagg gatatggctg ggaacatagc caacctaaaa
1801 atacagtcca agatgcatct catatcccca cgtatgcacc catcccagct taaccgcttt
1861 gtaaacactt acaccaaggg cctgcccctg gcaatcagct tgctactgaa agacattttt
1921 aggcaccacg cccagcgctc ctgctacgac tggatcatct acaacaccac cccgcagcat
1981 gaagctctgc agtggtgcta cctccacccc agagacgggc ttatgcccat gtatctgaac
2041 atccagagtc acctttacca cgtcctggaa aaaatacaca ggaccctcaa cgaccgagac
2101 cgctggtccc gggcctaccg cgcgcgcaaa acccctaaat aaagacagca agacacttgc
2161 ttgatccaaa tccaaacaga gtctggtttt ttatttatgt tttaaaccgc attgggaggg
2221 gaggaagcct tcagggcaga aacctgctgg cgcagatcca acagctgctg agaaacgaca
2281 ttaagttccc gggtcaaaga atccaattgt gccaaaagag ccgtcaactt gtcatcgcgg
2341 gcggatgaac gggaagctgc actgcttgca agcgggctca ggaaagcaaa gtcagtcaca
2401 atcccgcggg cggtggctgc agcggctgaa gcggcggcgg aggctgcagt ctccaacggc
2461 gttccagaca cggtctcgta ggtcaaggta gtagagtttg cgggcaggac ggggcgacca
2521 tcaatgctgg agcccatcac attctgacgc accccggccc atgggggcat gcgcgttgtc
2581 aaatatgagc tcacaatgct tccatcaaac gagttggtgc tcatggcggc ggcggctgct
2641 gcaaaacaga tacaaaacta cataagaccc ccaccttata tattctttcc cacccttaag
2701 ccacgcccac acatttcagt acctcaatct gtatcttcat cgctagagcc aaactcagcg
2761 cgggtgcagg ccagcaccaa gtgatcgggc ctcagctcct cggtcacatc cagcatcaca
2821 ggctggttcc taatatgttt accgccacac tcgcagggtc tgcacctggt gcgggtctca
2881 tcgtacctca gcaccttcca gatctgccac cgcggggact agagtcgacc tcatggctgc
2941 gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc tcccggcatc
3001 cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt tttcaccgtc
3061 atcaccgaaa cgcgcgaggc agccggatca taatcagcca taccacattt gtagaggttt
3121 tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
3181 ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
3241 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
3301 tcaatgtatc ttatcatgtc tggatctccc cgctgtggcg gccgcgaatt cgcccttccg
3361 ctcgagttac ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc
3421 cagcaggacc atgtgatcgc gcttctcgtt ggggtctttg ctcagggcgg agcaggtgct
3481 caggtagtgg ttgtcgggca gcagcacggg gccgtcgccg atgggggtgt tctgctggta
3541 gtggtcggcg agctgcacgc tgccgtcctc gatgttgtgg cggatcttga agttcacctt
3601 gatgccgttc ttctgcttgt cggccatgat atagacgttg tggcagttgt agttgtactc
3661 cagcttgtgc cccaggatgt tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg
3721 gttcaccagg gtgtcgccct cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc
3781 cttgaagaag atggtgcgct cctggacgta gccttcgggc atggcggact tgaagaagtc
3841 gtgccgcttc atgtggtcgg ggtagcggct gaagcactgc acgccgtagg tcagggtggt
3901 cacgagggtg ggccagggca cgggcagctt gccggtggtg ctgatgaact tcagggtcag
3961 cttgccgtag gtggcatcgc cctcgccctc gccggacacg ctgaacttgt ggccgtttac
4021 gtcgccgtcc agctcgacca ggatgggcac caccccggtg aacagctcct cgcccttgct
4081 caccatgatg ccgctgcgct tgaagaagcg gatgctctgg cgcaggctca gcatggtggc
4141 ggtaccatct gacggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc
4201 ctaccgccca tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga
4261 ttttggtgcc aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt
4321 gagtcaaacc gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata
4381 gcgatgacta atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg
4441 gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata
4501 tgatacactt gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt
4561 caatggaaag tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg
4621 cgggggtcgt tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgacctgc
4681 aggctgatct ccctagacaa atattacgcg ctatgagtaa cacaaaatta ttcagatttc
4741 acttcctctt attcagtttt cccgcgaaaa tggccaaatc ttactcggtt acgcccaaat
4801 ttactacaac atccgcctaa aaccgcgcga aaattgtcac ttcctgtgta caccggcgca
4861 caccaaaaac gtcacttttg ccacatccgt cgcttacatg tgttccgcca cacttgcaac
4921 atcacacttc cgccacacta ctacgtcacc cgccccgttc ccacgccccg cgccacgtca
4981 caaactccac cccctcatta tcatattggc ttcaatccaa aataaggtat attattgatg
5041 atgctagctt aattaattga agacgaaagg gcctcgtgat acgcctattt ttataggtta
5101 atgtcatgat aataatggtt tcttagacgt caggtggcac atttccccga aaagtgcgcg
5161 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
5221 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
5281 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
5341 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
5401 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
5461 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtgttgac gccgggcaag
5521 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
5581 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
5641 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
5701 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
5761 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgcagca atggcaacaa
5821 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
5881 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
5941 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
6001 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
6061 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
6121 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
6181 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
6241 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
6301 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
6361 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
6421 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
6481 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
6541 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
6601 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
6661 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
6721 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
6781 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
6841 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
6901 ttttacggtt cctggccttt tgctggcct
//
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