出品公司: | EMD Biosciences(Novagen) |
---|---|
别名: | pET26b, pet-26b(+), pET 26b(+) |
质粒类型: | 大肠杆菌蛋白表达 |
表达水平: | 高 |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 5360bp |
5' 测序引物: | T7 |
5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
载体标签: | C-His |
载体抗性: | 卡那(Kanamycin) |
备注: |
含有pelB信号肽,蛋白表达时,能够将目的基因定位于细胞周质。
|
产品目录号: | 69862-3 |
稳定性: | 瞬时表达 Transient |
组成型: | 组成型 Constitutive |
病毒/非病毒: | 非病毒 |
表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3) |
pET表达手册: |
pET表达手册英文版 pET表达手册中文版 |
pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET-26b(+)载体 pet26b质粒基本信息
pET-26b(+)质粒图谱 pet26b载体图谱和pET-26b(+)载体序列 pet26b质粒序列多克隆位点信息
Feature Name | Start | End |
---|---|---|
T7_terminator | 129 | 1 |
T7_Terminal_primer | 69 | 87 |
T7_transl_en_RBS | 314 | 298 |
lacO | 359 | 332 |
T7_promoter | 377 | 359 |
tet (300 - 563) | 409 | 672 |
pBRrevBam_primer | 480 | 461 |
lacI | 755 | 1846 |
tet (576 - 851) | 1905 | 2180 |
ROP | 2655 | 2846 |
pGEX_3_primer | 2862 | 2840 |
pBR322_origin | 3880 | 3261 |
KanR2 | 3986 | 4801 |
ORF | Start | End |
---|---|---|
ORF frame 1 | 514 | 1014 |
ORF frame 2 | 887 | 1846 |
ORF frame 2 | 3986 | 4801 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
NcoI | 220 |
MscI | 225 |
NdeI | 288 |
XbaI | 326 |
BglII | 392 |
BclI | 1128 |
ApaI | 1325 |
HpaI | 1620 |
FspI | 2196 |
NruI | 4074 |
ClaI | 4108 |
SmaI | 4291 |
XmaI | 4289 |
pET-26b(+)质粒 pet26b载体简介
pET-26b(+)质粒序列 pet26b载体序列
LOCUS pET-26b(+) 5360 bp DNA circular SYN DEFINITION pET-26b(+) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT ORIGDB|GenBank COMMENT VNTAUTHORNAME|biofeng.com| COMMENT VNTOAUTHORAD1|Novagen Inc.| FEATURES Location/Qualifiers source 1..5360 /organism="pET-26b(+)" /mol_type="other DNA" terminator complement(1..129) /label="T7_terminator" misc_feature 69..87 /label="T7_Terminal_primer" misc_feature complement(298..314) /label="T7_transl_en_RBS" misc_feature complement(332..359) /label="lacO" promoter complement(359..377) /label="T7_promoter" gene 409..672 /label="tet (300 - 563)" /gene="tet (300 - 563)" misc_feature complement(461..480) /label="pBRrevBam_primer" CDS 514..1014 /label="ORF frame 1" misc_feature 755..1846 /label="lacI" CDS 887..1846 /label="ORF frame 2" gene 1905..2180 /label="tet (576 - 851)" /gene="tet (576 - 851)" misc_feature 2655..2846 /label="ROP" misc_feature complement(2840..2862) /label="pGEX_3_primer" rep_origin complement(3261..3880) /label="pBR322_origin" gene 3986..4801 /label="KanR2" /gene="KanR2" CDS 3986..4801 /label="ORF frame 2" ORIGIN 1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 181 cgacggagct cgaattcgga tccgaattaa ttccgatatc catggccatc gccggctggg 241 cagcgaggag cagcagacca gcagcagcgg tcggcagcag gtatttcata tgtatatctc 301 cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc acaattcccc 361 tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc cggacgcatc 421 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc 481 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg 541 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt 601 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg 661 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat 721 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt 781 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca 841 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa 901 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt 961 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg 1021 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc 1081 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta 1141 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt 1201 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg 1261 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc 1321 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac 1381 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt 1441 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa 1501 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc 1561 ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt 1621 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca 1681 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag 1741 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt 1801 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta 1861 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca 1921 acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg 1981 tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg 2041 aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct 2101 tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc 2161 aggccattat cgccggcatg gcggccccac gggtgcgcat gatcgtgctc ctgtcgttga 2221 ggacccggct aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg 2281 agcgaacgtg aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg 2341 tcttcggttt ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta 2401 tgttccggat ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat 2461 taacgaagcg ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg 2521 ccagttgttt accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta 2581 tcgtgagcat cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt 2641 acacggaggc atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca 2701 gaagccagac attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag 2761 acatctgtga atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt 2821 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc 2881 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt 2941 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta 3001 tgcggcatca gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac 3061 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg 3121 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 3181 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag 3241 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac 3301 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga 3361 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt 3421 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc 3481 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc 3541 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta 3601 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat 3661 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca 3721 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct 3781 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt 3841 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct 3901 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta 3961 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag 4021 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa 4081 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt 4141 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact 4201 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga 4261 tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg tattagaaga 4321 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca 4381 ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc tcgctcaggc 4441 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg 4501 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc 4561 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat 4621 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct 4681 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg 4741 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta 4801 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg 4861 ttccgcgcac atttccccga aaagtgccac ctgaaattgt aaacgttaat attttgttaa 4921 aattcgcgtt aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca 4981 aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga 5041 acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc 5101 agggcgatgg cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc 5161 gtaaagcact aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc 5221 cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg 5281 caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac 5341 agggcgcgtc ccattcgcca //
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