| 出品公司: | Promega |
|---|---|
| 载体名称: | pGL4.11 ; pGL4.11[luc2P] |
| 质粒类型: | 信号通路报告载体；哺乳动物载体；萤火虫荧光素酶报告载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | 无 |
| 载体大小: | 4370 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | luc2P |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | 潮霉素(Hygromycin) |
| 克隆菌株: | TOP10等常规菌株 |
| 宿主细胞（系）: | HEK293等 |
| 备注: | pGL4.11[luc2P]载体是信号通路报告载体，无启动子，含萤火虫荧光 素酶报告基因luc2P |
| 产品目录号: | E666A |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pGL4.11[luc2P]载体pGL4.11质粒基本信息
pGL4.11[luc2P]质粒图谱pGL4.11载体图谱和pGL4.11[luc2P]载体序列pGL4.11质粒序列多克隆位点信息
pGL4.11[luc2P]质粒pGL4.11载体简介
The pGL4.11[luc2P] Vector encodes the luciferase reporter gene luc2P (Photinus pyralis) and is designed for high expression and reduced anomalous transcription. The pGL4 Vectors are engineered with fewer consensus regulatory sequences and a synthetic gene, which has been codon optimized for mammalian expression. The pGL4.11[luc2P] Vector is a basic vector with no promoter. However, it contains a multiple cloning region to allow cloning of a promoter of choice. The luc2P reporter gene contains hPEST, a protein destabilization sequence. The protein encoded by luc2P responds more quickly and with greater magnitude to changes in transcriptional activity than the luc2 gene, its more stable counterpart.
pGL4.11[luc2P]质粒序列pGL4.11载体序列
LOCUS AY738223 4370 bp DNA circular SYN 08-NOV-2004
DEFINITION Firefly luciferase reporter vector pGL4.11[luc2P], complete
sequence.
ACCESSION AY738223
VERSION AY738223.1 GI:55535621
KEYWORDS .
SOURCE Firefly luciferase reporter vector pGL4.11[luc2P]
ORGANISM Firefly luciferase reporter vector pGL4.11[luc2P]
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4370)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE pGL4 Luciferase Reporter Vectors Technical Manual, TM259, Promega
Corporation
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 4370)
AUTHORS Almond,B., Chauvin,F. and Browning,J.
TITLE Direct Submission
JOURNAL Submitted (31-AUG-2004) Scientific Communications, Promega
Corporation, 2800 Woods Hollow Rd., Madison, WI 53711, USA
FEATURES Location/Qualifiers
source 1..4370
/organism="Firefly luciferase reporter vector
pGL4.11[luc2P]"
/mol_type="other DNA"
/db_xref="taxon:296660"
misc_feature 1..70
/note="multiple cloning site region"
CDS 100..1875
/codon_start=1
/transl_table=11
/product="luciferase luc2P"
/protein_id="AAV52871.1"
/db_xref="GI:55535622"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTD
AHIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAV
APANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQG
FQSMYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVR
FSHARDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRS
LQDYKIQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLP
GIRQGYGLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCV
RGPMIMSGYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVA
PAELESILLQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTT
AKKLRGGVVFVDEVPKGLTGKLDARKIREILIKAKKGGKIAVNSHGFPPEVEEQAAGT
LPMSCAQESGMDRHPAACASARINV"
misc_feature 1915..2136
/note="SV40 late poly(A) region"
primer_bind 2204..2223
/note="reporter vector primer 4 (RVPrimer4)"
rep_origin 2461
/note="ColE1-derived"
gene complement(3252..4112)
/gene="ampR"
CDS complement(3252..4112)
/gene="ampR"
/note="AmpR"
/codon_start=1
/transl_table=11
/product="synthetic beta-lactamase"
/protein_id="AAV52872.1"
/db_xref="GI:55535623"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW"
regulatory 4217..4370
/regulatory_class="polyA_signal_sequence"
/note="synthetic; transcriptional pause site"
primer_bind complement(4217..4370)
/note="reporter vector primer 3 (RVPrimer3)"
ORIGIN
1 ggcctaactg gccggtacct gagctcgcta gcctcgagga tatcaagatc tggcctcggc
61 ggccaagctt ggcaatccgg tactgttggt aaagccacca tggaagatgc caaaaacatt
121 aagaagggcc cagcgccatt ctacccactc gaagacggga ccgccggcga gcagctgcac
181 aaagccatga agcgctacgc cctggtgccc ggcaccatcg cctttaccga cgcacatatc
241 gaggtggaca ttacctacgc cgagtacttc gagatgagcg ttcggctggc agaagctatg
301 aagcgctatg ggctgaatac aaaccatcgg atcgtggtgt gcagcgagaa tagcttgcag
361 ttcttcatgc ccgtgttggg tgccctgttc atcggtgtgg ctgtggcccc agctaacgac
421 atctacaacg agcgcgagct gctgaacagc atgggcatca gccagcccac cgtcgtattc
481 gtgagcaaga aagggctgca aaagatcctc aacgtgcaaa agaagctacc gatcatacaa
541 aagatcatca tcatggatag caagaccgac taccagggct tccaaagcat gtacaccttc
601 gtgacttccc atttgccacc cggcttcaac gagtacgact tcgtgcccga gagcttcgac
661 cgggacaaaa ccatcgccct gatcatgaac agtagtggca gtaccggatt gcccaagggc
721 gtagccctac cgcaccgcac cgcttgtgtc cgattcagtc atgcccgcga ccccatcttc
781 ggcaaccaga tcatccccga caccgctatc ctcagcgtgg tgccatttca ccacggcttc
841 ggcatgttca ccacgctggg ctacttgatc tgcggctttc gggtcgtgct catgtaccgc
901 ttcgaggagg agctattctt gcgcagcttg caagactata agattcaatc tgccctgctg
961 gtgcccacac tatttagctt cttcgctaag agcactctca tcgacaagta cgacctaagc
1021 aacttgcacg agatcgccag cggcggggcg ccgctcagca aggaggtagg tgaggccgtg
1081 gccaaacgct tccacctacc aggcatccgc cagggctacg gcctgacaga aacaaccagc
1141 gccattctga tcacccccga aggggacgac aagcctggcg cagtaggcaa ggtggtgccc
1201 ttcttcgagg ctaaggtggt ggacttggac accggtaaga cactgggtgt gaaccagcgc
1261 ggcgagctgt gcgtccgtgg ccccatgatc atgagcggct acgttaacaa ccccgaggct
1321 acaaacgctc tcatcgacaa ggacggctgg ctgcacagcg gcgacatcgc ctactgggac
1381 gaggacgagc acttcttcat cgtggaccgg ctgaagagcc tgatcaaata caagggctac
1441 caggtagccc cagccgaact ggagagcatc ctgctgcaac accccaacat cttcgacgcc
1501 ggggtcgccg gcctgcccga cgacgatgcc ggcgagctgc ccgccgcagt cgtcgtgctg
1561 gaacacggta aaaccatgac cgagaaggag atcgtggact atgtggccag ccaggttaca
1621 accgccaaga agctgcgcgg tggtgttgtg ttcgtggacg aggtgcctaa aggactgacc
1681 ggcaagttgg acgcccgcaa gatccgcgag attctcatta aggccaagaa gggcggcaag
1741 atcgccgtga attctcacgg cttccctccc gaggtggagg agcaggccgc cggcaccctg
1801 cccatgagct gcgcccagga gagcggcatg gatagacacc ctgctgcttg cgccagcgcc
1861 aggatcaacg tctaaggccg cgactctaga gtcggggcgg ccggccgctt cgagcagaca
1921 tgataagata cattgatgag tttggacaaa ccacaactag aatgcagtga aaaaaatgct
1981 ttatttgtga aatttgtgat gctattgctt tatttgtaac cattataagc tgcaataaac
2041 aagttaacaa caacaattgc attcatttta tgtttcaggt tcagggggag gtgtgggagg
2101 ttttttaaag caagtaaaac ctctacaaat gtggtaaaat cgataaggat ccgtcgaccg
2161 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc
2221 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg
2281 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
2341 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
2401 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
2461 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
2521 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
2581 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
2641 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
2701 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
2761 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
2821 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
2881 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
2941 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
3001 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
3061 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
3121 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
3181 taaatcaatc taaagtatat atgagtaaac ttggtctgac agcggccgca aatgctaaac
3241 cactgcagtg gttaccagtg cttgatcagt gaggcaccga tctcagcgat ctgcctattt
3301 cgttcgtcca tagtggcctg actccccgtc gtgtagatca ctacgattcg tgagggctta
3361 ccatcaggcc ccagcgcagc aatgatgccg cgagagccgc gttcaccggc ccccgatttg
3421 tcagcaatga accagccagc agggagggcc gagcgaagaa gtggtcctgc tactttgtcc
3481 gcctccatcc agtctatgag ctgctgtcgt gatgctagag taagaagttc gccagtgagt
3541 agtttccgaa gagttgtggc cattgctact ggcatcgtgg tatcacgctc gtcgttcggt
3601 atggcttcgt tcaactctgg ttcccagcgg tcaagccggg tcacatgatc acccatatta
3661 tgaagaaatg cagtcagctc cttagggcct ccgatcgttg tcagaagtaa gttggccgcg
3721 gtgttgtcgc tcatggtaat ggcagcacta cacaattctc ttaccgtcat gccatccgta
3781 agatgctttt ccgtgaccgg cgagtactca accaagtcgt tttgtgagta gtgtatacgg
3841 cgaccaagct gctcttgccc ggcgtctata cgggacaaca ccgcgccaca tagcagtact
3901 ttgaaagtgc tcatcatcgg gaatcgttct tcggggcgga aagactcaag gatcttgccg
3961 ctattgagat ccagttcgat atagcccact cttgcaccca gttgatcttc agcatctttt
4021 actttcacca gcgtttcggg gtgtgcaaaa acaggcaagc aaaatgccgc aaagaaggga
4081 atgagtgcga cacgaaaatg ttggatgctc atactcgtcc tttttcaata ttattgaagc
4141 atttatcagg gttactagta cgtctctcaa ggataagtaa gtaatattaa ggtacgggag
4201 gtattggaca ggccgcaata aaatatcttt attttcatta catctgtgtg ttggtttttt
4261 gtgtgaatcg atagtactaa catacgctct ccatcaaaac aaaacgaaac aaaacaaact
4321 agcaaaatag gctgtcccca gtgcaagtgc aggtgccaga acatttctct
//
