pEPSA5Cas9syn2

价格:1800元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

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载体基本信息

质粒名称: pEPSA5Cas9syn2
出品公司: Addgene
目录编号: 206963
存储实验室: Daijie Chen
体骨架基本信息
载体名称: pEPSA5
载体出品公司: Clontech
空载体大小: 5500bp
完整质粒大小: 10062bp
载体类型: Bacterial Expression, CRISPR, Synthetic Biology
载体抗性: 氨苄霉素
生长温度: 37 ℃
宿主菌: DH5a
拷贝数: 低拷贝

插入基因信息
克隆方法: Ligation Independent Cloning

文献引用方法
材料和方法部分: pEPSA5Cas9syn2 was a gift from Daijie Chen (Addgene plasmid # 206963 ; http://n2t.net/addgene:206963 ; RRID:Addgene_206963)
参考文献部分: Efficient Genome Editing in Most Staphylococcus aureus by Using the Restriction-Modification System Silent CRISPR-Cas9 Toolkit. Yang P, Yang J, Lin T, Liu Q, Yin Y, Chen D, Yang S. ACS Synth Biol. 2023 Oct 13. doi: 10.1021/acssynbio.3c00339. 10.1021/acssynbio.3c00339 PubMed 37830328

载体图谱质粒图谱和多克隆位点信息

pEPSA5Cas9syn2载体图谱

载体简介

载体序列

LOCUS       pEPSA5Cas9syn2               10062 bp ds-DNA     circular SYN 10-NOV-2023
DEFINITION  S. aureus genome editing vector removed these two motifs 
            AGGNNNNNGAT, CCAYNNNNNNTGT, CCAYNNNNNNGTA, GGANNNNNNNNTCG, and 
            GWAGNNNNNNGAT.
ACCESSION   .
VERSION     .
KEYWORDS    pEPSA5Cas9syn2
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10062)
  TITLE     Efficient Genome Editing in Most Staphylococcus aureus by Using the 
            Restriction-modification Systems Silent CRISPR-Cas9 Toolkit
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10062)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Nov 10, 2023 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10062
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(260..805)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(340..359)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_RNA        4102..4177
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     CDS             4418..8524
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(8555..8572)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      8725..8811
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      8903..8930
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     CDS             complement(9072..9932)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9695..9714
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9933..10037)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ggcggccgca ctggcttact atgttggcac tgatgagggt gtcagtgaag tgcttcatgt
       61 ggcaggagaa aaaaggctgc accggtgcgt cagcagaata tgtgatacag gatatattcc
      121 gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc ggaaatggct
      181 tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag ggaagtgaga
      241 gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat cacgaaatct
      301 gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag gcgtttcccc
      361 ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt gtcattccgc
      421 tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta ggcagttcgc
      481 tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc cttatccggt
      541 aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc agcagccact
      601 ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct aaactgaaag
      661 gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag agttggtagc
      721 tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag agcaagagat
      781 tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatg cggccgcttc tttcctgcgt
      841 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      901 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc
      961 ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataggtaga tccctcgacc
     1021 tgcaggcatg caagcttctg taggttttta ggcataaaac tatatgattt acccctaaat
     1081 ctttaaaatg ccccttaaaa ttcaaaataa aggcatttaa aatttaaata tttcttgtga
     1141 taaagtttgt taaaaaggag tggttttatg actgttatgt ggttatcgat tataggtatg
     1201 tggttttgta ttggaatggc attttttgct atcaaggtta ttaaaaataa aaattagacc
     1261 acgcatttat gccgagaaaa tttattgtgc gttgagaaga acccttaact aaacttgcag
     1321 acgaatgtcg gcatagcgtg agctattaag ccgaccattc gacaagtttt gggattgtta
     1381 agggttccga ggctcaacgt caataaagca attggaataa agaagcgaaa aaggagaagt
     1441 cggttcagaa aaagaagcat atgcatctgg agctgtaata taaaaacctt cttcaactaa
     1501 cggggcaggt tagtgacatt agaaaaccga ctgtaaaaag tacagtcggc attatctcat
     1561 attataaaag ccagtcatta ggcctatctg acaattcctg aatagagttc ataaacaatc
     1621 ctgcatgata accatcacaa acagaatgat gtacctgtaa agatagcggt aaatatattg
     1681 aattaccttt attaatgaat tttcctgctg taataatggg tagaaggtaa ttactattat
     1741 tattgatatt taagttaaac ccagtaaatg aagtccatgg aataatagaa agagaaaaag
     1801 cattttcagg tataggtgtt ttgggaaaca atttccccga accattatat ttctctacat
     1861 cagaaaggta taaatcataa aactctttga agtcattctt tacaggagtc caaataccag
     1921 agaatgtttt agatacacca tcaaaaattg tataaagtgg ctctaactta tcccaataac
     1981 ctaactctcc gtcgctattg taaccagttc taaaagctgt atttgagttt atcacccttg
     2041 tcactaagaa aataaatgca gggtaaaatt tatatccttc ttgttttatg tttcggtata
     2101 aaacactaat atcaatttct gtggttatac taaaagtcgt ttgttggttc aaataatgat
     2161 taaatatctc ttttctcttc caattgtcta aatcaatttt attaaagttc atttgatatg
     2221 cctcctaaat ttttatctaa agtgaattta ggaggcttac ttgtctgctt tcttcattag
     2281 aatcaatcct tttttaaaag tcaatattac tgtaacataa atatatattt taaaaatatc
     2341 ccactttatc caattttcgt ttgttgaact aatgggtgct ttagttgaag aataaaagac
     2401 cacattaaaa aatgtggtct tttgtgtttt tttaaaggat ttgagcgtag cgaaaaatcc
     2461 ttttctttct tatcttgata ataagggtaa ctattgccgg cgaggctagt tacccttaag
     2521 ttattggtat gactggtttt aagcgcaaaa aaagttgctt tttcgtacct attaatgtat
     2581 cgttttaaat gaatagtaaa aaacatacat agaaagggga aaaagcaact ttttttattg
     2641 tcatagtttg tgaaaactaa gttgttttta tgtgttataa catggaaaag tatactgaga
     2701 aaaaacaaag aaatcaagta tttcagaaat ttattaaacg tcatattgga gagaatcaaa
     2761 tggatttagt tgaagattgc aatacatttc tgtcttttgt agctgataaa actttagaaa
     2821 aacagaaatt atataaagct aattcttgta aaaatcgatt ttgtcctgtc tgtgcttgga
     2881 gaaaagctag aaaagatgca ttgggtttat ctttgatgat gcaatatatt aagcagcaag
     2941 agaaaaagga gtttatcttt ttaactttga ctacacctaa tgtaatgagt gatgaattag
     3001 aaaatgaaat aaaacgttat aataattctt ttagaaaact tataaagaga aaaaaagtag
     3061 gtagtgttat aaagggatat gttcgtaagt tagagattac atataataaa aaaagagatg
     3121 attataatcc tcattttcat gtgttaattg cagtaaataa atcgtatttc acagataaaa
     3181 gatattatat tagccaacaa gaatggttag atttatggcg tgatgtaacg ggcatttcag
     3241 aaataacaca agttcaagtt caaaaaataa gacaaaataa taataaagaa ttatatgaaa
     3301 tggctaagta ttctggtaaa gatagtgatt atttaataaa tcaaaaagtc tttgatgcat
     3361 tttataaatc acttaaaggt aaacaggtat tagtttattc aggattattt aaagaggcta
     3421 aaaagaaatt aaaaaatggg gatttagatt acttaaaaga aattgatcca accgaatata
     3481 tctatcaaat tttttatatt tggaaacaaa aagagtattt agctagtgaa ctttatgact
     3541 taacagaaca agaaaaaaga gaaattaatc acaaaatgat agacgaaatc gaggaagaac
     3601 aataacaaaa tataagtgct aacagtcgtc tgcaagttta gttaagggtt cttctcaacg
     3661 cacaataaat tttctcggca taaatgcgtg gtctaatttt tatttttaat aaccttgata
     3721 gcaaaaaatg ccattccaat acaaaaccac atacctataa tcgataacca cataacagtc
     3781 ataaaaccac tcctttttaa caaactttat cacaagaaat attttggcat tctacgacta
     3841 taacttaaat ttatattttt tactttataa tatataattg attatagaat aatgttgctc
     3901 atatcgtttg ccaacatcta gtactcaaat tacactatgt tacacttggt aatattaacc
     3961 gaacttcccc tgtccaaatt agtttttacg gttcctggcc ttttgctggc cttttgctca
     4021 catgttcttt cctgcgttgt cgacagagtt tgcaaaatat acaggggatt atatataatg
     4081 gaaacgagac cattggtctc agttttagag ctagaaatag caagttaaaa taaggctagt
     4141 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttgagatc tgtccatacc
     4201 catggtctag aatgctcgag tcagaaaaat atacctgtat cttttttcaa aagcaaacat
     4261 gctttgggta aacatgtagg tattaacgtc aatgcgacaa tagtagcatt gattaaatga
     4321 gaattagtaa gtgttttact tactaaattt tatttaacct aaaaatgaac cacctggatg
     4381 tgtgggatta aaaagtgaag agaggaggac gtatcacatg gataagaaat actcaatagg
     4441 cttagatatc ggcacaaata gcgtcggatg ggcggtgatc actgatgaat ataaggttcc
     4501 gtctaaaaag ttcaaggttc tgggaaatac agaccgccac agtatcaaaa aaaatcttat
     4561 aggggctctt ttatttgaca gtggagagac agcggaagcg actcgtctca aacggacagc
     4621 tcgtagaagg tatacacgtc ggaagaatcg tatttgttat ctacaggaga ttttttcaaa
     4681 tgagatggcg aaagtagatg atagtttctt tcatcgactt gaagagtctt ttttggtgga
     4741 agaagacaag aagcatgaac gtcatcctat ttttggaaat atagtagatg aagttgctta
     4801 tcatgagaaa tatccaacta tctatcatct gcgaaaaaaa ttggtagatt ctactgataa
     4861 agcggatttg cgcttaatct atttggcctt agcgcatatg attaagtttc gtggtcattt
     4921 tttgattgag ggagatttaa atcctgataa tagtgatgtg gacaaactat ttatccagtt
     4981 ggtacaaacc tacaatcaat tatttgaaga aaaccctatt aacgcaagtg gagtagatgc
     5041 taaagcgatt ctttctgcac gattgagtaa atcaagacga ttagaaaatc tcattgctca
     5101 gctccccggt gagaagaaaa atggcttatt tgggaatctc attgctttgt cattgggttt
     5161 gacccctaat tttaaatcaa attttgattt ggcagaagat gctaaattac agctttcaaa
     5221 agatacttac gatgatgatt tagataattt attggcgcaa attggagatc aatatgctga
     5281 tttgtttttg gcagctaaga atttatcaga tgctatttta ctttcagata tcctaagagt
     5341 aaatactgaa ataactaagg ctcccctatc agcttcaatg attaaacgct acgatgaaca
     5401 tcatcaagac ttgactcttt taaaagcttt agttcgacaa caacttccag aaaagtataa
     5461 agaaatcttt tttgatcaat caaaaaacgg atatgcaggt tatattgatg ggggagctag
     5521 ccaagaagaa ttttataaat ttatcaaacc aattttagaa aaaatggatg gtactgagga
     5581 attattggtg aaactaaatc gtgaagattt gctgcgcaag caacggacct ttgacaacgg
     5641 ctctattccc catcaaattc acttgggtga gctgcatgct attttgagaa gacaagaaga
     5701 cttttatcca tttttaaaag acaatcgtga gaagattgaa aaaatcttga cttttcgaat
     5761 tccttattat gttggtccat tggcgcgtgg caatagtcgt tttgcatgga tgactcggaa
     5821 gtctgaagaa acaattaccc catggaattt tgaagaggtt gtcgataaag gtgcttcagc
     5881 tcaatcattt attgaacgca tgacaaactt tgataaaaat cttccaaatg aaaaagtact
     5941 accaaaacat agtttgcttt atgagtattt tacggtttat aacgaattga caaaggtcaa
     6001 atatgttact gaaggaatgc gaaaaccagc atttctttca ggtgaacaga agaaagccat
     6061 tgttgattta ctcttcaaaa caaatcgaaa agtaaccgtt aagcaattaa aagaagatta
     6121 tttcaaaaaa atagaatgtt ttgatagtgt tgaaatttca ggagttgaag atagatttaa
     6181 tgcttcatta ggtacctacc atgatttgct aaaaattatt aaagataaag attttttgga
     6241 taatgaagaa aatgaagata tcttagagga tattgtttta acattgacct tatttgaaga
     6301 tagagagatg attgaggaaa gacttaaaac atatgctcac ctctttgatg ataaggtgat
     6361 gaaacagctt aaacgtcgcc gttatactgg ttggggacgt ttgtctcgaa aattgattaa
     6421 tggtattagg gataagcaat ctggcaaaac aatattagat tttttgaaat cagatggttt
     6481 tgccaatcgc aattttatgc agctgatcca tgatgatagt ttgacattta aagaagacat
     6541 tcaaaaagca caagtgtctg gacaaggcga tagtttacat gaacatattg caaatttagc
     6601 tggtagccct gctattaaaa aaggtatttt acagactgta aaagttgttg atgaattggt
     6661 caaagtaatg gggcggcata agccagaaaa tatcgttatt gaaatggcac gtgaaaatca
     6721 gacaactcaa aagggccaga aaaattcgcg agagcgtatg aaacgaatcg aagaaggtat
     6781 caaagaatta ggaagtcaga ttcttaaaga gcatcctgtt gaaaatactc aattgcaaaa
     6841 tgaaaagctc tatctctatt atctccaaaa tggaagagac atgtatgtgg accaagaatt
     6901 agatattaat cgtttaagtg attatgatgt cgatcacatt gttccacaaa gtttccttaa
     6961 agacgattca atagacaata aggtcttaac gcgttctgat aaaaatcgtg gtaaatcgga
     7021 taacgttcca agtgaagaag tagtcaaaaa gatgaaaaac tattggagac aacttctaaa
     7081 cgccaagtta atcactcaac gtaagtttga taatttaacg aaagctgaac gtggaggttt
     7141 gagtgaactt gataaagctg gttttatcaa acgccaattg gttgaaactc gccaaatcac
     7201 taagcatgtg gcacaaattt tggatagtcg catgaatact aaatacgatg aaaatgataa
     7261 acttattcga gaggttaaag tgattacctt aaaatctaaa ttagtttctg acttccgaaa
     7321 agattttcaa ttctataaag tacgtgagat taacaattac catcatgccc atgatgcgta
     7381 tctaaatgcc gtcgttggaa ctgctttgat taagaaatat ccaaaacttg aatcggagtt
     7441 tgtctatggt gattataaag tttatgatgt tcgtaaaatg attgctaagt ctgagcaaga
     7501 aataggcaaa gcaaccgcaa aatatttctt ttactctaat atcatgaact ttttcaaaac
     7561 agaaattaca cttgcaaatg gagagattcg caaacgccct ctaatcgaaa ctaatgggga
     7621 aactggagaa attgtctggg ataaagggcg agattttgcc acagtgcgca aagtattgtc
     7681 catgccccaa gtcaatattg tcaagaaaac agaagtacag acaggcggat tctccaagga
     7741 gtcaatttta ccaaaaagaa attcggacaa gcttattgct cgtaaaaaag actgggatcc
     7801 aaaaaaatat ggtggttttg atagtccaac ggtagcttat tcagtcctag tggttgctaa
     7861 ggtggaaaaa gggaaatcga agaagttaaa atccgttaaa gagttactag ggatcacaat
     7921 tatggaaaga agttcctttg aaaaaaatcc gattgacttt ttagaagcta aaggatataa
     7981 ggaagttaaa aaagacttaa tcattaaact acctaaatat agtctttttg agttagaaaa
     8041 cggtcgtaaa cggatgctgg ctagtgccgg agaattacaa aaaggaaatg agctggctct
     8101 gccaagcaaa tatgtgaatt ttttatattt agctagtcat tatgaaaagt tgaagggtag
     8161 tccagaagat aacgaacaaa aacaattgtt tgtggagcag cataagcatt atttagatga
     8221 gattattgag caaatcagtg aattttctaa gcgtgttatt ttagcagatg ccaatttaga
     8281 taaagttctt agtgcatata acaaacatag agacaaacca atacgtgaac aagcagaaaa
     8341 tattattcat ttatttacgt tgacgaatct tggagctccc gctgctttta aatattttga
     8401 tacaacaatt gatcgtaaac gatatacgtc tacaaaagag gttttagatg ccactcttat
     8461 ccatcaatcc atcactggtc tttatgaaac acgcattgat ttgagtcagc taggaggtga
     8521 ctaatcgacc tgcagccaag cttgggcttt tcagcctgat acagattaaa tcagaacgca
     8581 gaagcggtct gataaaacag aatttgcctg gcggcagtag cgcggtggtc ccacctgacc
     8641 ccatgccgaa ctcagaagtg aaacgccgta gcgccgatgg tagtgtggcg tctccccttg
     8701 cgagagtagg gaactgccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     8761 tttcgtttta tctgttgttt gtcggtgaac gctctcctga gtaggacaaa tccgccggga
     8821 gcggatttga acgttgcgaa gcaacggccc ggagggtggc gggcaggacg cccgccataa
     8881 actgccaggc atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta
     8941 caaactcttt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgcccatcgt
     9001 atcgatgata agctgtcaaa catgagaatt aaatcaatct aaagtatata tgagtaaact
     9061 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     9121 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     9181 ccatctggcc ccagtgctgc aatgataccg cgtgacccac gctcaccggc tccagattta
     9241 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     9301 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     9361 agtttgcgca acgttgttgc cattgctact ggcatcgtag tgtcacgctc gtcgtttggt
     9421 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     9481 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     9541 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     9601 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     9661 cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca tagcagaact
     9721 ttaaaagtgc tcatcattgg aaaacgctct tcggggcgaa aactctcaag gatcttaccg
     9781 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     9841 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     9901 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     9961 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
    10021 caaatagggg ttccgcgcac atttccccga aaagtgccac ct
//

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