| 质粒名称: | pGEN-MCS |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 44919 |
| 存储实验室: | Harry Mobley |
|
载体骨架基本信息 |
|
| 载体名称: | pGEN |
| 载体抗性: | 氨苄青霉素 |
| 完整质粒大小: | 5160 bp |
| 载体修饰: | 添加了多个克隆站点 |
| 载体类型: | stable complementation |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 克隆方法: | 限制性内切酶 |
| 5' 测序引物 | GGCACTTGCTCACGCTCTG |
| 3' 测序引物 | gtggtcacgcttttcgttgg |
|
文献引用方法 |
|
| 材料和方法部分: | pGEN-MCS was a gift from Harry Mobley (Addgene plasmid # 44919 ; http://n2t.net/addgene:44919 ; RRID:Addgene_44919) |
| 参考文献部分: | Expression of flagella is coincident with uropathogenic Escherichia coli ascension to the upper urinary tract. Lane MC, Alteri CJ, Smith SN, Mobley HL. Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007 Oct 9. 10.1073/pnas.0607898104 PubMed 17925449 |
买家导航
pGENMCS载体基本信息
pGENMCS载体图谱质粒图谱和多克隆位点信息
pGENMCS载体简介
pGENMCS载体序列
LOCUS pGEN-MCS 5160 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pGEN-MCS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5160)
AUTHORS Lane MC, Alteri CJ, Smith SN, Mobley HL
TITLE Expression of flagella is coincident with uropathogenic Escherichia
coli ascension to the upper urinary tract.
JOURNAL Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16669-74. Epub 2007
Oct 9.
PUBMED 17925449
REFERENCE 2 (bases 1 to 5160)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5160
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 132..152
/label=GFP-F
/note="GFP, forward primer. Does NOT anneal to EGFP"
rep_origin complement(283..828)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(363..382)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
terminator 903..930
/label=rrnB T2 terminator
/note="transcription terminator T2 from the E. coli rrnB
gene"
terminator 1022..1108
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
primer_bind 1261..1278
/label=pBAD Reverse
/note="For vectors with E. coli araBAD promoter, reverse
primer"
CDS complement(1823..2683)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 2446..2465
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(2684..2788)
/gene="bla"
/label=AmpR promoter
primer_bind 2856..2874
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 gaattcctgc agggcatgcc ccgggcatat gaagcttggt accgggatcc gcggccgcgg
61 gccccatgga tcgatagctg gtcgacacaa tctgcccttt cgaaagatcc caacgaaaag
121 cgtgaccaca tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat
181 gagctctaca aataatgagc tagcccgcct aatgagcggg cttttttttc tcggcctagg
241 agatacttaa cagggaagtg agagggccgc ggcaaagccg tttttccata ggctccgccc
301 ccctgacaag catcacgaaa tctgacgctc aaatcagtgg tggcgaaacc cgacaggact
361 ataaagatac caggcgtttc cccctggcgg ctccctcgtg cgctctcctg ttcctgcctt
421 tcggtttacc ggtgtcattc cgctgttatg gccgcgtttg tctcattcca cgcctgacac
481 tcagttccgg gtaggcagtt cgctccaagc tggactgtat gcacgaaccc cccgttcagt
541 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggaa agacatgcaa
601 aagcaccact ggcagcagcc actggtaatt gatttagagg agttagtctt gaagtcatgc
661 gccggttaag gctaaactga aaggacaagt tttggtgact gcgctcctcc aagccagtta
721 cctcggttca aagagttggt agctcagaga accttcgaaa aaccgccctg caaggcggtt
781 ttttcgtttt cagagcaaga gattacgcgc agaccaaaac gatctcaaga agatcatctt
841 attaatcaga taaaatattt ctaggatcta aaacactagg cccaagagtt tgtagaaacg
901 caaaaaggcc atccgtcagg atggccttct gcttaatttg atgcctggca gtttatggcg
961 ggcgtcctgc ccgccaccct ccgggccgtt gcttcgcaac gttcaaatcc gctcccggcg
1021 gatttgtcct actcaggaga gcgttcaccg acaaacaaca gataaaacga aaggcccagt
1081 ctttcgactg agcctttcgt tttatttgat gcctggcagt tccctactct cgcatgggga
1141 gaccccacac taccatcggc gctacggcgt ttcacttctg agttcggcat ggggtcaggt
1201 gggaccaccg cgctactgcc gccaggcaaa ttctgtttta tcagaccgct tctgcgttct
1261 gatttaatct gtatcaggct gaaaatcttc tctcatccgc caaaacagcc aagctggatc
1321 tggcaaatcg ctgaatattc cttttgtctc cgaccatcag gcacctgagt cgctgtcttt
1381 ttcgtgacat tcagttcgct gcgctcacgg ctctggcagt gaatgggggt aaatggcact
1441 acaggcgcct tttatggatt catgcaagga aactacccat aatacaagaa aagcccgtca
1501 cgggcttctc agggcgtttt atggcgggtc tgctatgtgg tgctatctga ctttttgctg
1561 ttcagcagtt cctgccctct gattttccag tctgaccact tcggattatc ccgtgacagg
1621 tcattcagac tggctaatgc acccagtaag gcagcggtat catcaacagg cttacccgtc
1681 ttactgtcaa ccggatctaa aacactagtt aagggatttt ggtcatgaga ttatcaaaaa
1741 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
1801 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
1861 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
1921 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
1981 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
2041 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
2101 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
2161 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
2221 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
2281 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
2341 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
2401 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
2461 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
2521 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
2581 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
2641 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
2701 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
2761 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
2821 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
2881 gtcttctaga caacatcagc aaggagaaag gggctaccgg cgaaccagca gcccctttat
2941 aaaggcgctt cagtagtcag accagcatca gtcctgaaaa ggcgggcctg cgcccgcctc
3001 caggttgcta cttaccggat tcgtaagcca tgaaagccgc cacctccctg tgtccgtctc
3061 tgtaacgaat ctcgcacagc gattttcgtg tcagataagt gaatatcaac agtgtgagac
3121 acacgatcaa cacacaccag acaagggaac ttcgtggtag tttcatggcc ttcttctcct
3181 tgcgcaaagc gcggtaagag gctatcctga tgtggactag acatagggat gcctcgtggt
3241 ggttaatgaa aattaactta ctacggggct atcttctttc tgccacacaa cacggcaaca
3301 aaccaccttc acgtcatgag gcagaaagcc tcaagcgccg ggcacatcat agcccatata
3361 cctgcacgct gaccacactc actttccctg aaaataatcc gctcattcag accgttcacg
3421 ggaaatccgt gtgattgttg ccgcatcacg ctgcctcccg gagtttgtct cgagcacttt
3481 tgttacccgc caaacaaaac ccaaaaacaa cccataccca acccaataaa acaccaaaac
3541 aagacaaata atcattgatt gatggttgaa atggggtaaa cttgacaaac aaacccactt
3601 aaaacccaaa acatacccaa acacacacca aaaaaacacc ataaggagtt ttataaatgt
3661 tggtattcat tgatgacggt tcaacaaaca tcaaactaca gtggcaggaa agcgacggaa
3721 caattaaaca gcacattagc ccgaacagct tcaaacgcga gtgggcagtc tcttttggtg
3781 ataaaaaggt ctttaactac acactgaacg gcgaacagta ttcatttgat ccaatcagcc
3841 cggatgctgt agtcacaacc aatatcgcat ggcaatacag cgacgttaat gtcgttgcag
3901 tgcatcacgc cttactgacc agtggtctgc cggtaagcga agtggatatt gtttgcacac
3961 ttcctctgac agagtattac gacagaaata accaacccaa tacggaaaat attgagcgta
4021 agaaagcaaa cttccggaaa aaaattacat taaatggcgg ggatacattc acaataaaag
4081 atgtaaaagt catgcctgaa tctataccgg caggttatga agttctacaa gaactggatg
4141 agttagattc tttattaatt atagatctcg ggggcaccac attagatatt tctcaggtaa
4201 tggggaaatt atcggggatc agtaaaatat acggagactc atctcttggt gtctctctgg
4261 ttacatctgc agtaaaagat gccctttctc ttgcgagaac aaaaggaagt agctatcttg
4321 ctgacgatat aatcattcac agaaaagata ataactatct gaagcaacga attaatgatg
4381 agaacaaaat atcaatagtc accgaagcaa tgaatgaagc acttcgtaaa cttgagcaac
4441 gtgtattaaa tacgctcaat gaattttctg gttatactca tgttatggtt ataggcggtg
4501 gcgcagaatt aatatgcgat gcagtaaaaa aacacacaca gattcgtgat gaacgttttt
4561 tcaaaaccaa taactctcaa tatgatttag ttaacggtat gtatctcata ggtaattaat
4621 gatggacaag cgcagaacca ttgccttcaa actaaatcca gatgtaaatc aaacagataa
4681 aattgtttgt gatacactgg acagtatccc gcaaggggaa cgaagccgcc ttaaccgggc
4741 cgcactgacg gcaggtctgg ccttatacag acaagatccc cggacccctt tccttttatg
4801 tgagctgctg acgaaagaaa ccacattttc agatatcgtg aatatattga gatcgctatt
4861 tccaaaagag atggccgatt ttaattcttc aatagtcact caatcctctt cacaacaaga
4921 gcaaaaaagt gatgaagaga ccaaaaaaaa tgcgatgaag ctaataaatt aattcaatta
4981 ttattgagtt ccctttatcc actatcaggc tggataaagg gaactcaatc aagttatttt
5041 cttaccagtc attacataat cgttattatg aaataatcgt ttgcactgtc tctgttattc
5101 aggcaatttc aataaaggca cttgctcacg ctctgtcatt ttctgaaact cttcatgctg
//
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