出品公司: | Agilent |
---|---|
载体名称: | pShuttle-CMV-lacZ, pShuttle CMV lacZ |
质粒类型: | 腺病毒表达载体,哺乳动物细胞表达载体 |
高拷贝/低拷贝: | 低拷贝 |
启动子: | CMV |
克隆方法: | 多克隆位点,限制性内切酶 |
载体大小: | 10660 bp |
5' 测序引物及序列: | pShuttle-CMV-F: GGTCTATATAAGCAGAGCTG |
3' 测序引物及序列: | pShuttle-CMV-R: GTGGTATGGCTGATTATGATCAG |
载体标签: | -- |
载体抗性: | 卡那 |
备注: | LacZ基因插入到pShuttle-CMV载体多克隆 位点。 |
产品目录号: | 240008 |
稳定性: | / |
组成型: | -- |
病毒/非病毒: | 腺病毒 |
买家导航
pShuttle-CMV-lacZ载体质粒基本信息
pShuttle-CMV-lacZ质粒图谱载体图谱和pShuttle-CMV-lacZ载体序列质粒序列多克隆位点信息
pShuttle-CMV-lacZ质粒载体简介
LacZ基因插入到了pshuttle-CMV载体的多克隆位点,构建成为pShuttle-CMV-lacZ质粒。该质粒可以作为实验中的重组腺病毒对照阳性质粒。
pShuttle-CMV-lacZ质粒序列载体序列
LOCUS pShuttle-CMV-lacZ 10660 bp DNA circular SYN DEFINITION pShuttle-CMV lacZ ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. COMMENT This file is created by Vector NTI http://www.biofeng.com/ COMMENT VNTAUTHORNAME|biofeng.com| FEATURES Location/Qualifiers source 1..10660 /organism="pShuttle-CMV lacZ" /mol_type="other DNA" misc_feature complement(1..103) /label="RITR" misc_feature 1..103 /label="LITR" misc_feature 183..331 /label="Encap" promoter 347..899 /label="CMV_immearly_promoter" misc_feature 402..689 /label="CAG_enhancer" misc_feature 856..876 /label="CMV_fwd_primer" promoter 857..926 /label="CMV_promoter" CDS 967..4017 /label="ORF frame 1" gene 970..4014 /label="lacZ" /gene="lacZ" misc_feature complement(972..994) /label="M13_pUC_fwd_primer" terminator 4216..4444 /label="SV40_PA_terminator" misc_feature 4414..4433 /label="EBV_rev_primer" CDS complement(5009..6367) /label="ORF frame 1" CDS complement(6115..6636) /label="ORF frame 2" misc_feature complement(7620..7722) /label="LITR" misc_feature 7620..7722 /label="RITR" rep_origin complement(7967..8586) /label="pBR322_origin" gene complement(8741..9052) /label="Ampicillin (311 - 0)" /gene="Ampicillin (311 - 0)" promoter 9264..9313 /label="NEOKAN_promoter" CDS 9402..10196 /label="ORF frame 3" gene 9405..10193 /label="NeoR/KanR" /gene="NeoR/KanR" ORIGIN 1 CATCATCAAT AATATACCTT ATTTTGGATT GAAGCCAATA TGATAATGAG GGGGTGGAGT 61 TTGTGACGTG GCGCGGGGCG TGGGAACGGG GCGGGTGACG TAGTAGTGTG GCGGAAGTGT 121 GATGTTGCAA GTGTGGCGGA ACACATGTAA GCGACGGATG TGGCAAAAGT GACGTTTTTG 181 GTGTGCGCCG GTGTACACAG GAAGTGACAA TTTTCGCGCG GTTTTAGGCG GATGTTGTAG 241 TAAATTTGGG CGTAACCGAG TAAGATTTGG CCATTTTCGC GGGAAAACTG AATAAGAGGA 301 AGTGAAATCT GAATAATTTT GTGTTACTCA TAGCGCGTAA NNNNTAATAG TAATCAATTA 361 CGGGGTCATT AGTTCATAGC CCATATATGG AGTTCCGCGT TACATAACTT ACGGTAAATG 421 GCCCGCCTGG CTGACCGCCC AACGACCCCC GCCCATTGAC GTCAATAATG ACGTATGTTC 481 CCATAGTAAC GCCAATAGGG ACTTTCCATT GACGTCAATG GGTGGAGTAT TTACGGTAAA 541 CTGCCCACTT GGCAGTACAT CAAGTGTATC ATATGCCAAG TACGCCCCCT ATTGACGTCA 601 ATGACGGTAA ATGGCCCGCC TGGCATTATG CCCAGTACAT GACCTTATGG GACTTTCCTA 661 CTTGGCAGTA CATCTACGTA TTAGTCATCG CTATTACCAT GGTGATGCGG TTTTGGCAGT 721 ACATCAATGG GCGTGGATAG CGGTTTGACT CACGGGGATT TCCAAGTCTC CACCCCATTG 781 ACGTCAATGG GAGTTTGTTT TGGCACCAAA ATCAACGGGA CTTTCCAAAA TGTCGTAACA 841 ACTCCGCCCC ATTGACGCAA ATGGGCGGTA GGCGTGTACG GTGGGAGGTC TATATAAGCA 901 GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGAGATCTGG TACCGTCGAC GCGGCCGCTC 961 GAGACCATGG TCGTTTTACA ACGTCGTGAC TGGGAAAACC CTGGCGTTAC CCAACTTAAT 1021 CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA GCGAAGAGGC CCGCACCGAT 1081 CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGC GCTTTGCCTG GTTTCCGGCA 1141 CCAGAAGCGG TGCCGGAAAG CTGGCTGGAG TGCGATCTTC CTGAGGCCGA TACTGTCGTC 1201 GTCCCCTCAA ACTGGCAGAT GCACGGTTAC GATGCGCCCA TCTACACCAA CGTGACCTAT 1261 CCCATTACGG TCAATCCGCC GTTTGTTCCC ACGGAGAATC CGACGGGTTG TTACTCGCTC 1321 ACATTTAATG TTGATGAAAG CTGGCTACAG GAAGGCCAGA CGCGAATTAT TTTTGATGGC 1381 GTTAACTCGG CGTTTCATCT GTGGTGCAAC GGGCGCTGGG TCGGTTACGG CCAGGACAGT 1441 CGTTTGCCGT CTGAATTTGA CCTGAGCGCA TTTTTACGCG CCGGAGAAAA CCGCCTCGCG 1501 GTGATGGTGC TGCGTTGGAG TGACGGCAGT TATCTGGAAG ATCAGGATAT GTGGCGGATG 1561 AGCGGCATTT TCCGTGACGT CTCGTTGCTG CATAAACCGA CTACACAAAT CAGCGATTTC 1621 CATGTTGCCA CTCGCTTTAA TGATGATTTC AGCCGCGCTG TACTGGAGGC TGAAGTTCAG 1681 ATGTGCGGCG AGTTGCGTGA CTACCTACGG GTAACAGTTT CTTTATGGCA GGGTGAAACG 1741 CAGGTCGCCA GCGGCACCGC GCCTTTCGGC GGTGAAATTA TCGATGAGCG TGGTGGTTAT 1801 GCCGATCGCG TCACACTACG TCTGAACGTC GAAAACCCGA AACTGTGGAG CGCCGAAATC 1861 CCGAATCTCT ATCGTGCGGT GGTTGAACTG CACACCGCCG ACGGCACGCT GATTGAAGCA 1921 GAAGCCTGCG ATGTCGGTTT CCGCGAGGTG CGGATTGAAA ATGGTCTGCT GCTGCTGAAC 1981 GGCAAGCCGT TGCTGATTCG AGGCGTTAAC CGTCACGAGC ATCATCCTCT GCATGGTCAG 2041 GTCATGGATG AGCAGACGAT GGTGCAGGAT ATCCTGCTGA TGAAGCAGAA CAACTTTAAC 2101 GCCGTGCGCT GTTCGCATTA TCCGAACCAT CCGCTGTGGT ACACGCTGTG CGACCGCTAC 2161 GGCCTGTATG TGGTGGATGA AGCCAATATT GAAACCCACG GCATGGTGCC AATGAATCGT 2221 CTGACCGATG ATCCGCGCTG GCTACCGGCG ATGAGCGAAC GCGTAACGCG AATGGTGCAG 2281 CGCGATCGTA ATCACCCGAG TGTGATCATC TGGTCGCTGG GGAATGAATC AGGCCACGGC 2341 GCTAATCACG ACGCGCTGTA TCGCTGGATC AAATCTGTCG ATCCTTCCCG CCCGGTGCAG 2401 TATGAAGGCG GCGGAGCCGA CACCACGGCC ACCGATATTA TTTGCCCGAT GTACGCGCGC 2461 GTGGATGAAG ACCAGCCCTT CCCGGCTGTG CCGAAATGGT CCATCAAAAA ATGGCTTTCG 2521 CTACCTGGAG AGACGCGCCC GCTGATCCTT TGCGAATACG CCCACGCGAT GGGTAACAGT 2581 CTTGGCGGTT TCGCTAAATA CTGGCAGGCG TTTCGTCAGT ATCCCCGTTT ACAGGGCGGC 2641 TTCGTCTGGG ACTGGGTGGA TCAGTCGCTG ATTAAATATG ATGAAAACGG CAACCCGTGG 2701 TCGGCTTACG GCGGTGATTT TGGCGATACG CCGAACGATC GCCAGTTCTG TATGAACGGT 2761 CTGGTCTTTG CCGACCGCAC GCCGCATCCA GCGCTGACGG AAGCAAAACA CCAGCAGCAG 2821 TTTTTCCAGT TCCGTTTATC CGGGCAAACC ATCGAAGTGA CCAGCGAATA CCTGTTCCGT 2881 CATAGCGATA ACGAGCTCCT GCACTGGATG GTGGCGCTGG ATGGTAAGCC GCTGGCAAGC 2941 GGTGAAGTGC CTCTGGATGT CGCTCCACAA GGTAAACAGT TGATTGAACT GCCTGAACTA 3001 CCGCAGCCGG AGAGCGCCGG GCAACTCTGG CTCACAGTAC GCGTAGTGCA ACCGAACGCG 3061 ACCGCATGGT CAGAAGCCGG GCACATCAGC GCCTGGCAGC AGTGGCGTCT GGCGGAAAAC 3121 CTCAGTGTGA CGCTCCCCGC CGCGTCCCAC GCCATCCCGC ATCTGACCAC CAGCGAAATG 3181 GATTTTTGCA TCGAGCTGGG TAATAAGCGT TGGCAATTTA ACCGCCAGTC AGGCTTTCTT 3241 TCACAGATGT GGATTGGCGA TAAAAAACAA CTGCTGACGC CGCTGCGCGA TCAGTTCACC 3301 CGTGCACCGC TGGATAACGA CATTGGCGTA AGTGAAGCGA CCCGCATTGA CCCTAACGCC 3361 TGGGTCGAAC GCTGGAAGGC GGCGGGCCAT TACCAGGCCG AAGCAGCGTT GTTGCAGTGC 3421 ACGGCAGATA CACTTGCTGA TGCGGTGCTG ATTACGACCG CTCACGCGTG GCAGCATCAG 3481 GGGAAAACCT TATTTATCAG CCGGAAAACC TACCGGATTG ATGGTAGTGG TCAAATGGCG 3541 ATTACCGTTG ATGTTGAAGT GGCGAGCGAT ACACCGCATC CGGCGCGGAT TGGCCTGAAC 3601 TGCCAGCTGG CGCAGGTAGC AGAGCGGGTA AACTGGCTCG GATTAGGGCC GCAAGAAAAC 3661 TATCCCGACC GCCTTACTGC CGCCTGTTTT GACCGCTGGG ATCTGCCATT GTCAGACATG 3721 TATACCCCGT ACGTCTTCCC GAGCGAAAAC GGTCTGCGCT GCGGGACGCG CGAATTGAAT 3781 TATGGCCCAC ACCAGTGGCG CGGCGACTTC CAGTTCAACA TCAGCCGCTA CAGTCAACAG 3841 CAACTGATGG AAACCAGCCA TCGCCATCTG CTGCACGCGG AAGAAGGCAC ATGGCTGAAT 3901 ATCGACGGTT TCCATATGGG GATTGGTGGC GACGACTCCT GGAGCCCGTC AGTATCGGCG 3961 GAATTCCAGC TGAGCGCCGG TCGCTACCAT TACCAGTTGG TCTGGTGTCA AAAATAAGGA 4021 TCCGGTTATT TTCCACCATA TTGCCGTCTT TTGGCAATGT GAGGGCCCGG AAACCTGGCC 4081 CTGTCTTCTT GACGAGCATT CCTAGGGGTC TTTCCCCTCT CGCCAAAGGA ATGCAAGGTC 4141 TGTTGAATGT CGTGAAGGAA GCAGTTCCTC TGGAAGCTTC TAGATAAGAT ATCCGATCCA 4201 CCGGATCTAG ATAACTGATC ATAATCAGCC ATACCACATT TGTAGAGGTT TTACTTGCTT 4261 TAAAAAACCT CCCACACCTC CCCCTGAACC TGAAACATAA AATGAATGCA ATTGTTGTTG 4321 TTAACTTGTT TATTGCAGCT TATAATGGTT ACAAATAAAG CAATAGCATC ACAAATTTCA 4381 CAAATAAAGC ATTTTTTTCA CTGCATTCTA GTTGTGGTTT GTCCAAACTC ATCAATGTAT 4441 CTTAACGCNN NNTAAGGGTG GGAAAGAATA TATAAGGTGG GGGTCTTATG TAGTTTTGTA 4501 TCTGTTTTGC AGCAGCCGCC GCCGCCATGA GCACCAACTC GTTTGATGGA AGCATTGTGA 4561 GCTCATATTT GACAACGCGC ATGCCCCCAT GGGCCGGGGT GCGTCAGAAT GTGATGGGCT 4621 CCAGCATTGA TGGTCGCCCC GTCCTGCCCG CAAACTCTAC TACCTTGACC TACGAGACCG 4681 TGTCTGGAAC GCCGTTGGAG ACTGCAGCCT CCGCCGCCGC TTCAGCCGCT GCAGCCACCG 4741 CCCGCGGGAT TGTGACTGAC TTTGCTTTCC TGAGCCCGCT TGCAAGCAGT GCAGCTTCCC 4801 GTTCATCCGC CCGCGATGAC AAGTTGACGG CTCTTTTGGC ACAATTGGAT TCTTTGACCC 4861 GGGAACTTAA TGTCGTTTCT CAGCAGCTGT TGGATCTGCG CCAGCAGGTT TCTGCCCTGA 4921 AGGCTTCCTC CCCTCCCAAT GCGGTTTAAA ACATAAATAA AAAACCAGAC TCTGTTTGGA 4981 TTTGGATCAA GCAAGTGTCT TGCTGTCTTT ATTTAGGGGT TTTGCGCGCG CGGTAGGCCC 5041 GGGACCAGCG GTCTCGGTCG TTGAGGGTCC TGTGTATTTT TTCCAGGACG TGGTAAAGGT 5101 GACTCTGGAT GTTCAGATAC ATGGGCATAA GCCCGTCTCT GGGGTGGAGG TAGCACCACT 5161 GCAGAGCTTC ATGCTGCGGG GTGGTGTTGT AGATGATCCA GTCGTAGCAG GAGCGCTGGG 5221 CGTGGTGCCT AAAAATGTCT TTCAGTAGCA AGCTGATTGC CAGGGGCAGG CCCTTGGTGT 5281 AAGTGTTTAC AAAGCGGTTA AGCTGGGATG GGTGCATACG TGGGGATATG AGATGCATCT 5341 TGGACTGTAT TTTTAGGTTG GCTATGTTCC CAGCCATATC CCTCCGGGGA TTCATGTTGT 5401 GCAGAACCAC CAGCACAGTG TATCCGGTGC ACTTGGGAAA TTTGTCATGT AGCTTAGAAG 5461 GAAATGCGTG GAAGAACTTG GAGACGCCCT TGTGACCTCC AAGATTTTCC ATGCATTCGT 5521 CCATAATGAT GGCAATGGGC CCACGGGCGG CGGCCTGGGC GAAGATATTT CTGGGATCAC 5581 TAACGTCATA GTTGTGTTCC AGGATGAGAT CGTCATAGGC CATTTTTACA AAGCGCGGGC 5641 GGAGGGTGCC AGACTGCGGT ATAATGGTTC CATCCGGCCC AGGGGCGTAG TTACCCTCAC 5701 AGATTTGCAT TTCCCACGCT TTGAGTTCAG ATGGGGGGAT CATGTCTACC TGCGGGGCGA 5761 TGAAGAAAAC GGTTTCCGGG GTAGGGGAGA TCAGCTGGGA AGAAAGCAGG TTCCTGAGCA 5821 GCTGCGACTT ACCGCAGCCG GTGGGCCCGT AAATCACACC TATTACCGGG TGCAACTGGT 5881 AGTTAAGAGA GCTGCAGCTG CCGTCATCCC TGAGCAGGGG GGCCACTTCG TTAAGCATGT 5941 CCCTGACTCG CATGTTTTCC CTGACCAAAT CCGCCAGAAG GCGCTCGCCG CCCAGCGATA 6001 GCAGTTCTTG CAAGGAAGCA AAGTTTTTCA ACGGTTTGAG ACCGTCCGCC GTAGGCATGC 6061 TTTTGAGCGT TTGACCAAGC AGTTCCAGGC GGTCCCACAG CTCGGTCACC TGCTCTACGG 6121 CATCTCGATC CAGCATATCT CCTCGTTTCG CGGGTTGGGG CGGCTTTCGC TGTACGGCAG 6181 TAGTCGGTGC TCGTCCAGAC GGGCCAGGGT CATGTCTTTC CACGGGCGCA GGGTCCTCGT 6241 CAGCGTAGTC TGGGTCACGG TGAAGGGGTG CGCTCCGGGC TGCGCGCTGG CCAGGGTGCG 6301 CTTGAGGCTG GTCCTGCTGG TGCTGAAGCG CTGCCGGTCT TCGCCCTGCG CGTCGGCCAG 6361 GTAGCATTTG ACCATGGTGT CATAGTCCAG CCCCTCCGCG GCGTGGCCCT TGGCGCGCAG 6421 CTTGCCCTTG GAGGAGGCGC CGCACGAGGG GCAGTGCAGA CTTTTGAGGG CGTAGAGCTT 6481 GGGCGCGAGA AATACCGATT CCGGGGAGTA GGCATCCGCG CCGCAGGCCC CGCAGACGGT 6541 CTCGCATTCC ACGAGCCAGG TGAGCTCTGG CCGTTCGGGG TCAAAAACCA GGTTTCCCCC 6601 ATGCTTTTTG ATGCGTTTCT TACCTCTGGT TTCCATGAGC CGGTGTCCAC GCTCGGTGAC 6661 GAAAAGGCTG TCCGTGTCCC CGTATACAGA CTTGAGAGGG AGTTTAAACG AATTCAATAG 6721 CTTGTTGCAT GGGCGGCGAT ATAAAATGCA AGGTGCTGCT CAAAAAATCA GGCAAAGCCT 6781 CGCGCAAAAA AGAAAGCACA TCGTAGTCAT GCTCATGCAG ATAAAGGCAG GTAAGCTCCG 6841 GAACCACCAC AGAAAAAGAC ACCATTTTTC TCTCAAACAT GTCTGCGGGT TTCTGCATAA 6901 ACACAAAATA AAATAACAAA AAAACATTTA AACATTAGAA GCCTGTCTTA CAACAGGAAA 6961 AACAACCCTT ATAAGCATAA GACGGACTAC GGCCATGCCG GCGTGACCGT AAAAAAACTG 7021 GTCACCGTGA TTAAAAAGCA CCACCGACAG CTCCTCGGTC ATGTCCGGAG TCATAATGTA 7081 AGACTCGGTA AACACATCAG GTTGATTCAT CGGTCAGTGC TAAAAAGCGA CCGAAATAGC 7141 CCGGGGGAAT ACATACCCGC AGGCGTAGAG ACAACATTAC AGCCCCCATA GGAGGTATAA 7201 CAAAATTAAT AGGAGAGAAA AACACATAAA CACCTGAAAA ACCCTCCTGC CTAGGCAAAA 7261 TAGCACCCTC CCGCTCCAGA ACAACATACA GCGCTTCACA GCGGCAGCCT AACAGTCAGC 7321 CTTACCAGTA AAAAAGAAAA CCTATTAAAA AAACACCACT CGACACGGCA CCAGCTCAAT 7381 CAGTCACAGT GTAAAAAAGG GCCAAGTGCA GAGCGAGTAT ATATAGGACT AAAAAATGAC 7441 GTAACGGTTA AAGTCCACAA AAAACACCCA GAAAACCGCA CGCGAACCTA CGCCCAGAAA 7501 CGAAAGCCAA AAAACCCACA ACTTCCTCAA ATCGTCACTT CCGTTTTCCC ACGTTACGTA 7561 ACTTCCCATT TTAAGAAAAC TACAATTCCC AACACATACA AGTTACTCCG CCCTAAAACC 7621 TACGTCACCC GCCCCGTTCC CACGCCCCGC GCCACGTCAC AAACTCCACC CCCTCATTAT 7681 CATATTGGCT TCAATCCAAA ATAAGGTATA TTATTGATGA TGTTAATTAA CATGCATGGA 7741 TCCATATGCG GTGTGAAATA CCGCACAGAT GCGTAAGGAG AAAATACCGC ATCAGGCGCT 7801 CTTCCGCTTC CTCGCTCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG CGAGCGGTAT 7861 CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA 7921 ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT 7981 TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT 8041 GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC 8101 GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA 8161 GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT 8221 CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA 8281 ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG 8341 GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC 8401 CTAACTACGG CTACACTAGA AGGACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA 8461 CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG 8521 GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT 8581 TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG 8641 TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA 8701 AATCAATCTA AAGTATATAT GAGTAAACTT GGTCTGACAG TTACCAATGC TTAATCAGTG 8761 AGGCACCTAT CTCAGCGATC TGTCTATTTC GTTCATCCAT AGTTGCCTGA CTCCCCGTCG 8821 TGTAGATAAC TACGATACGG GAGGGCTTAC CATCTGGCCC CAGTGCTGCA ATGATACCGC 8881 GAGACCCACG CTCACCGGCT CCAGATTTAT CAGCAATAAA CCAGCCAGCC GGAAGGGCCG 8941 AGCGCAGAAG TGGTCCTGCA ACTTTATCCG CCTCCATCCA GTCTATTAAT TGTTGCCGGG 9001 AAGCTAGAGT AAGTAGTTCG CCAGTTAATA GTTTGCGCAA CGTTGTTGCC ATTGCTGCAG 9061 CCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT CACGTAGAAA GCCAGTCCGC 9121 AGAAACGGTG CTGACCCCGG ATGAATGTCA GCTACTGGGC TATCTGGACA AGGGAAAACG 9181 CAAGCGCAAA GAGAAAGCAG GTAGCTTGCA GTGGGCTTAC ATGGCGATAG CTAGACTGGG 9241 CGGTTTTATG GACAGCAAGC GAACCGGAAT TGCCAGCTGG GGCGCCCTCT GGTAAGGTTG 9301 GGAAGCCCTG CAAAGTAAAC TGGATGGCTT TCTTGCCGCC AAGGATCTGA TGGCGCAGGG 9361 GATCAAGCTC TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT 9421 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC 9481 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC 9541 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAAGACGAG GCAGCGCGGC 9601 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG 9661 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC 9721 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG 9781 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC 9841 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC 9901 CAGCCGAACT GTTCGCCAGG CTCAAGGCGA GCATGCCCGA CGGCGAGGAT CTCGTCGTGA 9961 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA 10021 TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG GCTACCCGTG 10081 ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT TACGGTATCG 10141 CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC TTCTGAATTT 10201 TGTTAAAATT TTTGTTAAAT CAGCTCATTT TTTAACCAAT AGGCCGAAAT CGGCAACATC 10261 CCTTATAAAT CAAAAGAATA GACCGCGATA GGGTTGAGTG TTGTTCCAGT TTGGAACAAG 10321 AGTCCACTAT TAAAGAACGT GGACTCCAAC GTCAAAGGGC GAAAAACCGT CTATCAGGGC 10381 GATGGCCCAC TACGTGAACC ATCACCCAAA TCAAGTTTTT TGCGGTCGAG GTGCCGTAAA 10441 GCTCTAAATC GGAACCCTAA AGGGAGCCCC CGATTTAGAG CTTGACGGGG AAAGCCGGCG 10501 AACGTGGCGA GAAAGGAAGG GAAGAAAGCG AAAGGAGCGG GCGCTAGGGC GCTGGCAAGT 10561 GTAGCGGTCA CGCTGCGCGT AACCACCACA CCCGCGCGCT TAATGCGCCG CTACAGGGCG 10621 CGTCCATTCG CCATTCAGGA TCGAATTAAT TCTTAATTAA //
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pShuttle-CMV-lacZ质粒载体应用举例
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