质粒名称: | pCAGIG |
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出品公司: | 爱迪基因 |
目录编号: | 11159 |
存储实验室: | Connie Cepko |
载体骨架基本信息 |
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载体名称: | pCAGEN |
载体抗性: | 氨苄霉素 |
空载体大小: | 4817 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | IRES-EGFP |
插入大小: | 1318 bp |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | NotI(未破坏) |
3' 克隆位点: | MscI(未破坏) |
5' 测序引物 | pCAG-F |
3' 测序引物 | |
文献引用方法 |
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材料和方法部分: | pCAGIG was a gift from Connie Cepko (Addgene plasmid # 11159) |
参考文献部分: | Electroporation and RNA interference in the rodent retina in vivo and in vitro. Matsuda T, Cepko CL. Proc Natl Acad Sci U S A. 2004 Jan 6. 101(1):16-22. 10.1073/pnas.2235688100 PubMed 14603031 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAGIG 6135 bp DNA SYN DEFINITION pCAGIG ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..6135 /organism="pCAGIG" /mol_type="other DNA" misc_feature 84..371 /label="CAG_enhancer" CDS 255..734 /label="ORF frame 3" CDS complement(802..1533) /label="ORF frame 1" CDS complement(843..1346) /label="ORF frame 2" misc_feature 1778..2350 /label="IRES" CDS 2342..3073 /label="ORF frame 2" gene 2357..3070 /label="EGFP" /gene="EGFP" misc_feature complement(2399..2420) /label="EGFP_N_primer" misc_feature 2537..2566 /label="Y66 (EGFP)" misc_feature 3007..3028 /label="EGFP_C_primer" terminator 3127..3592 /label="rb_glob_PA_terminator" promoter complement(3634..3652) /label="M13_reverse_primer" misc_feature complement(3651..3673) /label="M13_pUC_rev_primer" promoter complement(3687..3716) /label="lac_promoter" promoter 3822..4001 /label="SV40_promoter" rep_origin 3857..3934 /label="SV40_origin" misc_feature 3919..3938 /label="SV40pro_F_primer" terminator 4015..4146 /label="SV40_PA_terminator" misc_feature 4103..4122 /label="EBV_rev_primer" rep_origin complement(4370..4989) /label="pBR322_origin" gene complement(5144..6004) /label="Ampicillin" /gene="Ampicillin" CDS complement(5144..6004) /label="ORF frame 3" promoter complement(6046..6074) /label="AmpR_promoter" ORIGIN 1 GTCGACATTG ATTATTGACT AGTTATTAAT AGTAATCAAT TACGGGGTCA TTAGTTCATA 61 GCCCATATAT GGAGTTCCGC GTTACATAAC TTACGGTAAA TGGCCCGCCT GGCTGACCGC 121 CCAACGACCC CCGCCCATTG ACGTCAATAA TGACGTATGT TCCCATAGTA ACGCCAATAG 181 GGACTTTCCA TTGACGTCAA TGGGTGGACT ATTTACGGTA AACTGCCCAC TTGGCAGTAC 241 ATCAAGTGTA TCATATGCCA AGTACGCCCC CTATTGACGT CAATGACGGT AAATGGCCCG 301 CCTGGCATTA TGCCCAGTAC ATGACCTTAT GGGACTTTCC TACTTGGCAG TACATCTACG 361 TATTAGTCAT CGCTATTACC ATGGGTCGAG GTGAGCCCCA CGTTCTGCTT CACTCTCCCC 421 ATCTCCCCCC CCTCCCCACC CCCAATTTTG TATTTATTTA TTTTTTAATT ATTTTGTGCA 481 GCGATGGGGG CGGGGGGGGG GGGGGCGCGC GCCAGGCGGG GCGGGGCGGG GCGAGGGGCG 541 GGGCGGGGCG AGGCGGAGAG GTGCGGCGGC AGCCAATCAG AGCGGCGCGC TCCGAAAGTT 601 TCCTTTTATG GCGAGGCGGC GGCGGCGGCG GCCCTATAAA AAGCGAAGCG CGCGGCGGGC 661 GGGAGTCGCT GCGTTGCCTT CGCCCCGTGC CCCGCTCCGC GCCGCCTCGC GCCGCCCGCC 721 CCGGCTCTGA CTGACCGCGT TACTCCCACA GGTGAGCGGG CGGGACGGCC CTTCTCCTCC 781 GGGCTGTAAT TAGCGCTTGG TTTAATGACG GCTCGTTTCT TTTCTGTGGC TGCGTGAAAG 841 CCTTAAAGGG CTCCGGGAGG GCCCTTTGTG CGGGGGGGAG CGGCTCGGGG GGTGCGTGCG 901 TGTGTGTGTG CGTGGGGAGC GCCGCGTGCG GCCCGCGCTG CCCGGCGGCT GTGAGCGCTG 961 CGGGCGCGGC GCGGGGCTTT GTGCGCTCCG CGTGTGCGCG AGGGGAGCGC GGCCGGGGGC 1021 GGTGCCCCGC GGTGCGGGGG GGCTGCGAGG GGAACAAAGG CTGCGTGCGG GGTGTGTGCG 1081 TGGGGGGGTG AGCAGGGGGT GTGGGCGCGG CGGTCGGGCT GTAACCCCCC CCTGCACCCC 1141 CCTCCCCGAG TTGCTGAGCA CGGCCCGGCT TCGGGTGCGG GGCTCCGTGC GGGGCGTGGC 1201 GCGGGGCTCG CCGTGCCGGG CGGGGGGTGG CGGCAGGTGG GGGTGCCGGG CGGGGCGGGG 1261 CCGCCTCGGG CCGGGGAGGG CTCGGGGGAG GGGCGCGGCG GCCCCGGAGC GCCGGCGGCT 1321 GTCGAGGCGC GGCGAGCCGC AGCCATTGCC TTTTATGGTA ATCGTGCGAG AGGGCGCAGG 1381 GACTTCCTTT GTCCCAAATC TGGCGGAGCC GAAATCTGGG AGGCGCCGCC GCACCCCCTC 1441 TAGCGGGCGC GGGCGAAGCG GTGCGGCGCC GGCAGGAAGG AAATGGGCGG GGAGGGCCTT 1501 CGTGCGTCGC CGCGCCGCCG TCCCCTTCTC CATCTCCAGC CTCGGGGCTG CCGCAGGGGG 1561 ACGGCTGCCT TCGGGGGGGA CGGGGCAGGG CGGGGTTCGG CTTCTGGCGT GTGACCGGCG 1621 GCTCTAGAGC CTCTGCTAAC CATGTTCATG CCTTCTTCTT TTTCCTACAG CTCCTGGGCA 1681 ACGTGCTGGT TGTTGTGCTG TCTCATCATT TTGGCAAAGA ATTCCTCGAG GATATCgcgg 1741 ccgctacgta aattccgccc cccccccccc tctccctccc ccccccctaa cgttactggc 1801 cgaagccgct tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg 1861 ccgtcttttg gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct 1921 aggggtcttt cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca 1981 gttcctctgg aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg 2041 aaccccccac ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct 2101 gcaaaggcgg cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa 2161 tggctctcct caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt 2221 atgggatctg atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa 2281 aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac acgatgataa 2341 tatggccaca accatggtga gcaagggcga ggagctgttc accggggtgg tgcccatcct 2401 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg 2461 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt 2521 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc 2581 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga 2641 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga 2701 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa 2761 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga 2821 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag 2881 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct 2941 gcccgacaac cactacctga gcacccagtc cgccctgagc aaagacccca acgagaagcg 3001 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga 3061 gctgtacaag taaagcggcc ggccgccagc acagtggtcg atcgacctgc aggcatgcaa 3121 gcttcagCCA ATGCCCTGGC TCACAAATAC CACTGAGATC TTTTTCCCTC TGCCAAAAAT 3181 TATGGGGACA TCATGAAGCC CCTTGAGCAT CTGACTTCTG GCTAATAAAG GAAATTTATT 3241 TTCATTGCAA TAGTGTGTTG GAATTTTTTG TGTCTCTCAC TCGGAAGGAC ATATGGGAGG 3301 GCAAATCATT TAAAACATCA GAATGAGTAT TTGGTTTAGA GTTTGGCAAC ATATGCCATA 3361 TGCTGGCTGC CATGAACAAA GGTGGCTATA AAGAGGTCAT CAGTATATGA AACAGCCCCC 3421 TGCTGTCCAT TCCTTATTCC ATAGAAAAGC CTTGACTTGA GGTTAGATTT TTTTTATATT 3481 TTGTTTTGTG TTATTTTTTT CTTTAACATC CCTAAAATTT TCCTTACATG TTTTACTAGC 3541 CAGATTTTTC CTCCTCTCCT GACTACTCCC AGTCATAGCT GTCCCTCTTC TCTTATGAAG 3601 ATCCCTCGAC CTGCAGCCCA AGCTTGGCGT AATCATGGTC ATAGCTGTTT CCTGTGTGAA 3661 ATTGTTATCC GCTCACAATT CCACACAACA TACGAGCCGG AAGCATAAAG TGTAAAGCCT 3721 GGGGTGCCTA ATGAGTGAGC TAACTCACAT TAATTGCGTT GCGCTCACTG CCCGCTTTCC 3781 AGTCGGGAAA CCTGTCGTGC CAGCGGATCC GCATCTCAAT TAGTCAGCAA CCATAGTCCC 3841 GCCCCTAACT CCGCCCATCC CGCCCCTAAC TCCGCCCAGT TCCGCCCATT CTCCGCCCCA 3901 TGGCTGACTA ATTTTTTTTA TTTATGCAGA GGCCGAGGCC GCCTCGGCCT CTGAGCTATT 3961 CCAGAAGTAG TGAGGAGGCT TTTTTGGAGG CCTAGGCTTT TGCAAAAAGC TAACTTGTTT 4021 ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA CAAATTTCAC AAATAAAGCA 4081 TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA TCAATGTATC TTATCATGTC 4141 TGGATCCGCT GCATTAATGA ATCGGCCAAC GCGCGGGGAG AGGCGGTTTG CGTATTGGGC 4201 GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT CGTTCGGCTG CGGCGAGCGG 4261 TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA 4321 AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG 4381 CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA 4441 GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG 4501 TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG 4561 GAAGCGTGGC GCTTTCTCAA TGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC 4621 GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG 4681 GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA 4741 CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT 4801 GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG 4861 TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG 4921 GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC 4981 CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT 5041 TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT 5101 TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA CAGTTACCAA TGCTTAATCA 5161 GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC CATAGTTGCC TGACTCCCCG 5221 TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG CCCCAGTGCT GCAATGATAC 5281 CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT AAACCAGCCA GCCGGAAGGG 5341 CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT CCAGTCTATT AATTGTTGCC 5401 GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG CAACGTTGTT GCCATTGCTA 5461 CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC ATTCAGCTCC GGTTCCCAAC 5521 GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA AGCGGTTAGC TCCTTCGGTC 5581 CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC ACTCATGGTT ATGGCAGCAC 5641 TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT TTCTGTGACT GGTGAGTACT 5701 CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG TTGCTCTTGC CCGGCGTCAA 5761 TACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT GCTCATCATT GGAAAACGTT 5821 CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG ATCCAGTTCG ATGTAACCCA 5881 CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC CAGCGTTTCT GGGTGAGCAA 5941 AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC GACACGGAAA TGTTGAATAC 6001 TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG 6061 GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC 6121 GAAAAGTGCC ACCTG //
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