| 出品公司: | Agilent |
|---|---|
| 载体名称: | pCMV-MCS， pCMVMCS, pCMV MCS |
| 质粒类型: | 腺病毒相关载体 |
| 表达水平: | 高 |
| 启动子: | CMV |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4495 bp |
| 5' 测序引物及序列: | Beta-globin-F：ATTCTGAGTCCAAGCTAGGC |
| 3' 测序引物及序列: | hGH-PA-R: CCAGCTTGGTTCCCAATAGA |
| 载体标签: | -- |
| 载体抗性: | 氨苄 |
| 备注: | AAV-Helper Free 腺病毒基因表达系统 |
| 产品目录号: | 240071 |
| 稳定性: | / |
| 组成型: | -- |
| 病毒/非病毒: | 腺病毒 |
买家导航
pCMV-MCS载体 pCMVMCS质粒基本信息
pCMV-MCS质粒图谱 pCMVMCS载体图谱和pCMV-MCS载体序列 pCMVMCS质粒序列多克隆位点信息
pCMV-MCS质粒 pCMVMCS载体简介
pCMV-MCS质粒序列 pCMVMCS载体序列
LOCUS pCMV-MCS 4495 bp DNA circular SYN 22-JUL-2001
DEFINITION Cloning vector pCMV-MCS, complete sequence.
ACCESSION AF369966
VERSION AF369966.1 GI:14994098
KEYWORDS .
SOURCE Cloning vector pCMV-MCS
ORGANISM Cloning vector pCMV-MCS
other sequences; artificial sequences; vectors.
REFERENCE 1 (bases 1 to 4495)
AUTHORS Grafsky,A.J. III.
TITLE pCMV-MCS for use in the AAV Helper-Free System
JOURNAL Unpublished
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT VNTAUTHORNAME|biofeng.com|
FEATURES Location/Qualifiers
source 1..4495
/organism="Cloning vector pCMV-MCS"
/mol_type="genomic DNA"
/db_xref="taxon:161367"
/lab_host="Escherichia coli"
promoter 1..670
/label="CMV promoter"
misc_feature 678..1170
/label="similar to beta-globin intron"
misc_feature 1178..1253
/label="MCS"
misc_feature 1249..1735
/label="hGH/poly A"
rep_origin complement(1844..2701)
/label="ColE1 origin"
CDS complement(2711..3571)
/label="confers ampicillin resistance"
/codon_start=1
/transl_table=11
/product="beta-lactamase"
/protein_id="AAK76424.1"
/db_xref="GI:14994099"
rep_origin complement(3952..4413)
/label="f1 origin"
ORIGIN
1 gcggccgcac gcgtggagct agttattaat agtaatcaat tacggggtca ttagttcata
61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgtcaatag
181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat
421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
481 tttgcaccaa aatcaacggg actttccaaa atgtcgtaac aactccgccc cattgacgca
541 aatgggcggt aggcgtgtac ggtgggaggt ctatataagc agagctcgtt tagtgaaccg
601 tcagatcgcc tggagacgcc atccacgctg ttttgacctc catagaagac accgggaccg
661 atccagcctc cgcggattcg aatcccggcc gggaacggtg cattggaacg cggattcccc
721 gtgccaagag tgacgtaagt accgcctata gagtctatag gcccacaaaa aatgctttct
781 tcttttaata tacttttttg tttatcttat ttctaatact ttccctaatc tctttctttc
841 agggcaataa tgatacaatg tatcatgcct ctttgcacca ttctaaagaa taacagtgat
901 aatttctggg ttaaggcaat agcaatattt ctgcatataa atatttctgc atataaattg
961 taactgatgt aagaggtttc atattgctaa tagcagctac aatccagcta ccattctgct
1021 tttattttat ggttgggata aggctggatt attctgagtc caagctaggc ccttttgcta
1081 atcatgttca tacctcttat cttcctccca cagctcctgg gcaacgtgct ggtctgtgtg
1141 ctggcccatc actttggcaa agaattggga ttcgaacatc gattgaattc cccggggatc
1201 ctctagagtc gacctgcaga agcttgcctc gagcagcgct gctcgagaga tctacgggtg
1261 gcatccctgt gacccctccc cagtgcctct cctggccctg gaagttgcca ctccagtgcc
1321 caccagcctt gtcctaataa aattaagttg catcattttg tctgactagg tgtccttcta
1381 taatattatg gggtggaggg gggtggtatg gagcaagggg caagttggga agacaacctg
1441 tagggcctgc ggggtctatt gggaaccaag ctggagtgca gtggcacaat cttggctcac
1501 tgcaatctcc gcctcctggg ttcaagcgat tctcctgcct cagcctcccg agttgttggg
1561 attccaggca tgcatgacca ggctcagcta atttttgttt ttttggtaga gacggggttt
1621 caccatattg gccaggctgg tctccaactc ctaatctcag gtgatctacc caccttggcc
1681 tcccaaattg ctgggattac aggcgtgaac cactgctccc ttccctgtcc ttctgatttt
1741 gtaggtaacc acgtgcggac cgagcggccg cgaccgcttc ctcgctcact gactcgctgc
1801 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
1861 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
1921 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
1981 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
2041 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
2101 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta
2161 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
2221 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
2281 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
2341 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat
2401 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
2461 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc
2521 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt
2581 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct
2641 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt
2701 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
2761 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
2821 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
2881 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
2941 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
3001 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
3061 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
3121 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
3181 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
3241 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
3301 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
3361 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
3421 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
3481 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
3541 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca
3601 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac
3661 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta
3721 ttatcatgac attaacctat aaaaataggc gtatcacgag gccctttcgt ctcgcgcgtt
3781 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc
3841 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
3901 gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg caccataaaa
3961 ttgtaaacgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
4021 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag cccgagatag
4081 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
4141 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcacccaaat
4201 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
4261 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
4321 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
4381 ccgccgcgct taatgcgccg ctacagggcg cgtactatgg ttgctttgac gtatgcggtg
4441 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctggc ctaaa
//
