质粒名称: | pMaster12 |
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出品公司: | 爱迪基因 |
目录编号: | 58527 |
存储实验室: | Mario Capecchi |
载体骨架基本信息 |
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载体名称: | pCEP4 |
载体出品公司: | -- |
载体抗性: | 氨苄青霉素 |
空载体大小: | -- |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418),tk |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | 816 bp |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | -- |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | pMaster12 was a gift from Mario Capecchi (Addgene plasmid # 58527 ; http://n2t.net/addgene:58527 ; RRID:Addgene_58527) |
参考文献部分: | Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. Wu S, Wu Y, Zhang X, Capecchi MR. Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. 10.1073/pnas.1409933111 PubMed 25002522 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMaster12 27879 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Reprogram somatic cells to iPS cells. ACCESSION . VERSION . KEYWORDS pMaster12 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 27879) AUTHORS Wu S, Wu Y, Zhang X, Capecchi MR TITLE Efficient germ-line transmission obtained with transgene-free induced pluripotent stem cells. JOURNAL Proc Natl Acad Sci U S A. 2014 Jul 22;111(29):10678-83. doi: 10.1073/pnas.1409933111. Epub 2014 Jul 7. PUBMED 25002522 REFERENCE 2 (bases 1 to 27879) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..27879 /organism="synthetic DNA construct" /mol_type="other DNA" polyA_signal 87..134 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" promoter complement(1891..3069) /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 1900..2838 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" polyA_signal 3090..3137 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" CDS complement(3369..4172) /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(3480..3499) /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind 4090..4109 /label=Neo-R /note="Neomycin resistance gene, reverse primer" misc_feature 4173..4759 /label=IRES2 /note="internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV)" primer_bind complement(4346..4365) /label=IRES-F /note="IRES internal ribosome entry site, forward primer" primer_bind 4575..4592 /label=IRES reverse /note="IRES internal ribosome entry site, reverse primer. Also called pCDH-rev" CDS complement(4775..5905) /codon_start=1 /product="herpes simplex virus thymidine kinase" /label=HSV TK /note="confers sensitivity to nucleoside analogs such as acyclovir or ganciclovir" /translation="MASYPGHQHASAFDQAARSRGHSNRRTALRPRRQQEATEVRPEQK MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRVLGASETIANIYTTQ HRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGSSHAPPPALTLIFDR HPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGALPEDRHIDRLAKRQR PGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAVPPQGAEPQSNAGPR PHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFILDYDQSPAGCRDAL LQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN" intron 5978..6986 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" promoter complement(6987..7262) /label=chicken beta-actin promoter enhancer 7265..7568 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" rep_origin 7694..8149 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(7781..7800) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 7991..8012 /label=F1ori-F /note="F1 origin, forward primer" polyA_signal 8156..8277 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" CDS complement(8401..9720) /codon_start=1 /product="human c-Myc proto-oncogene" /label=c-Myc /translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA" CDS complement(9721..9780) /codon_start=1 /product="2A peptide from equine rhinitis A virus polyprotein" /label=E2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="QCTNYALLKLAGDVESNPGP" CDS complement(10741..10794) /codon_start=1 /product="2A peptide from Thosea asigna virus capsid protein" /label=T2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="EGRGSLLTCGDVEENPGP" CDS complement(12214..12279) /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /label=F2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" regulatory 13365..13374 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" promoter complement(13382..14560) /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 13391..14329 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" enhancer 14667..14970 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 14973..15248 /label=chicken beta-actin promoter intron 15249..16257 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" polyA_signal 17251..17372 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" rep_origin 17852..19641 /label=oriP /note="Epstein-Barr virus oriP replication origin (Yates et al., 2000)" CDS complement(19943..21868) /codon_start=1 /product="Epstein-Barr nuclear antigen 1, also known as EBNA-1" /label=EBNA1 /note="crucial for latent viral infection, and for episomal amplification of vectors containing the oriP origin (Okita et al., 2013)" /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR GRGRGGGRPGAPGGSGSGPRHRDGVRRPQKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE GEEGQE" primer_bind complement(22303..22321) /label=pBRforEco /note="pBR322 vectors, upsteam of EcoRI site, forward primer" promoter 22389..22493 /gene="bla" /label=AmpR promoter CDS 22494..23354 /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind complement(22712..22731) /label=Amp-R /note="Ampicillin resistance gene, reverse primer" rep_origin 23525..24113 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 24014..24033 /label=pBR322ori-F /note="pBR322 origin, forward primer" polyA_signal 24344..24568 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind 24557..24574 /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" CDS complement(25230..25295) /codon_start=1 /product="2A peptide from foot-and-mouth disease virus polyprotein" /label=F2A /note="Eukaryotic ribosomes fail to insert a peptide bond between the Gly and Pro residues, yielding separate polypeptides." /translation="VKQTLNFDLLKLAGDVESNPGP" intron 26288..27296 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" promoter complement(27297..27572) /label=chicken beta-actin promoter enhancer 27575..75 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag 61 tcaataatca atgtcgaggg cgcgccgaac aaacgaccca acaccgtgcg ttttattctg 121 tctttttatt gccgtcatag cgcgggttcc ttccggtatt gtctccttcc gtgtttcagt 181 tagcctcccc ctagggtggg cgaagaactc cagcatgaga tccccgcgct ggaggatcat 241 ccagccggcg tcccggaaaa cgattccgaa gcccaacctt tcatagaagg cggcggtgga 301 atcgaaatct cgtgatggca ggttgggcgt cgcttggtcg gtcatttcga accccagagt 361 cccgcggccg cgtcgacatt taaatttatg ctcttttggc atgcaacatt tcaatgagaa 421 ggttattata gggcacatcc ccgttcaggt gcttgtagta gaggtattct tcagcctgca 481 tactgatggc ccggatttcg ggtagtcgaa gaagtagctg tccaaatttc tctgtctgct 541 gcgggtagtt acacattgtg tagtccagca gggcggcatt gacttgttcc tggacacctt 601 ctaccagctg gaagttttca aggtttttga catctaaact aaagagcacc aagaatttca 661 gacatacgaa ctctcgttga tcaaactgga gagaacgaag ttttgccact aactcctgtg 721 catgactcat gaggttgttg agggtggctc cggcttgtga tgctattatg gaatagtcca 781 cttgttgccc agtaaccagg aagatggatc cttcctttcc atgtaccact tgtcggtaaa 841 tgtggtcgag gattaagagc tcactccagc agttctgaag cagcttcatt tggtcatcaa 901 ccttaagttc tctgaagaag atactactcc tggcccactc gacaatggag aagagagttt 961 gatctgccat tttgcacata agcccaaagg tgctcagctt ttcgtgcttg cttcggttag 1021 cctgctcttg ctgcaaatag gccatgattt tagcctggac ttgaggctca tctggctcac 1081 acttcaaaag ttccagtatc agatgtggga tgcttgctgg agagctcgtc tggtaactat 1141 ccatatatga atagcccatt atggactcgg gtgagctggt ataggggtct gggtactcag 1201 acttgatggc ccggctagga aagtggccat atgtttggta accttgcagg ctgccgtgag 1261 ggggcattgt catgctaatg ggggatgtta caaagggact tctgtcatag tctgtaggag 1321 gcaaggcagc atggttcaga ggtaggcctt tggaggcaga gtggatgttt tgaattgcag 1381 aggaaatggt caggtcagag ggcatagctt ggatcacctg agacatggct tctagcttaa 1441 gtccattggc tcggatgagg gcttttttct gttgcttcag ggccctgtct ctcttgtaca 1501 ttggcccaaa cttattcctt cctccacgca ttcggtcggc ccttacagct tctagcttca 1561 ttccaacact tagacatttt tgaaaacgac agtaaggaca acgctttctc tgtgttttgt 1621 caatttggca gttctggttt tctatacatg tgtacctttt attattttgg actgttcgct 1681 taaaaaatcc cttgcagctt tcacaggtga ggagcccata atggtaccca gacactttat 1741 ctccacacac gggacaaagc tcttccagat cttcatcata ggagtaattc accattttaa 1801 attgagacac aataggtgta agtccgtgct ttaaagactc ttgtaaatcc ccagtatctg 1861 aattagaaga catggtggcg atcgctagct tcacgacacc tgaaatggaa gaaaaaaact 1921 ttgaaccact gtctgaggct tgagaatgaa ccaagatcca aactcaaaaa gggcaaattc 1981 caaggagaat tacatcaagt gccaagctgg cctaacttca gtctccaccc actcagtgtg 2041 gggaaactcc atcgcataaa acccctcccc ccaacctaaa gacgacgtac tccaaaagct 2101 cgagaactaa tcgaggtgcc tggacggcgc ccggtactcc gtggagtcac atgaagcgac 2161 ggctgaggac ggaaaggccc ttttcctttg tgtgggtgac tcacccgccc gctctcccga 2221 gcgccgcgtc ctccattttg agctccctgc agcagggccg ggaagcggcc atctttccgc 2281 tcacgcaact ggtgccgacc gggccagcct tgccgcccag ggcggggcga tacacggcgg 2341 cgcgaggcca ggcaccagag caggccggcc agcttgagac tacccccgtc cgattctcgg 2401 tggccgcgct cgcaggcccc gcctcgccga acatgtgcgc tgggacgcac gggccccgtc 2461 gccgcccgcg gccccaaaaa ccgaaatacc agtgtgcaga tcttggcccg catttacaag 2521 actatcttgc cagaaaaaaa gcgtcgcagc aggtcatcaa aaattttaaa tggctagaga 2581 cttatcgaaa gcagcgagac aggcgcgaag gtgccaccag attcgcacgc ggcggcccca 2641 gcgcccaggc caggcctcaa ctcaagcacg aggcgaaggg gctccttaag cgcaaggcct 2701 cgaactctcc cacccacttc caacccgaag ctcgggatca agaatcacgt actgcagcca 2761 ggtggaagta attcaaggca cgcaagggcc ataacccgta aagaggccag gcccgcggga 2821 accacacacg gcacttacct gtgttctggc ggcaaacccg ttgcgaaaaa gaacgttcac 2881 ggcgactact gcacttatat acggttctcc cccaccctcg ggaaaaaggc ggagccagta 2941 cacgacatca ctttcccagt ttaccccgcg ccaccttctc taggcaccgg ttcaattgcc 3001 gacccctccc cccaacttct cggggactgt gggcgatgtg cgctctgccc actgacgggc 3061 accggagcct cacgattaat cgccgcgccg aacaaacgac ccaacaccgt gcgttttatt 3121 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc 3181 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat 3241 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt 3301 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag 3361 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga 3421 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca 3481 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag 3541 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca 3601 tgggtcacga cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg 3661 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc 3721 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc 3781 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga 3841 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt 3901 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac 3961 gatagccgcg ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca 4021 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt 4081 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg 4141 tgcaatccat cttgttcaat ggcggatccc atggttgtgg ccatattatc atcgtgtttt 4201 tcaaaggaaa accacgtccc cgtggttcgg ggggcctaga cgttttttta acctcgacta 4261 aacacatgta aagcatgtgc accgaggccc cagatcagat cccatacaat ggggtacctt 4321 ctgggcatcc ttcagcccct tgttgaatac gcttgaggag agccatttga ctctttccac 4381 aactatccaa ctcacaacgt ggcactgggg ttgtgccgcc tttgcaggtg tatcttatac 4441 acgtggcttt tggccgcaga ggcacctgtc gccaggtggg gggttccgct gcctgcaaag 4501 ggtcgctaca gacgttgttt gtcttcaaga agcttccaga ggaactgctt ccttcacgac 4561 attcaacaga ccttgcattc ctttggcgag aggggaaaga cccctaggaa tgctcgtcaa 4621 gaagacaggg ccaggtttcc gggccctcac attgccaaaa gacggcaata tggtggaaaa 4681 taacatatag acaaacgcac accggcctta ttccaagcgg cttcggccag taacgttagg 4741 ggggggggag ggagaggggc ggatccgcgg ccgctcagtt agcctccccc atctcccggg 4801 caaacgtgcg cgccaggtcg catatcgtcg gtatggagcc gggggtggtg acgtgggtct 4861 ggaccatccc ggaggtaagt tgcagcaggg cgtcccggca gccggcgggc gattggtcgt 4921 aatccaggat aaagacgtgc atggaacgga ggcgtttggc caagacgtcc aaggcccagg 4981 caaacacgtt atacaggtcg ccgttggggg ccagcaactc gggggcccga aacagggtaa 5041 ataacgtgtc cccgatatgg ggtcgtgggc ccgcgttgct ctggggctcg gcaccctggg 5101 gcggcacggc cgtccccgaa agctgtcccc agtcctcccg ccacgacccg ccgcactgca 5161 gataccgcac cgtattggca agtagcccgt aaacgcggcg aatcgcagcc agcatagcca 5221 ggtccagccg ctcgccgggg cgctggcgtt tggccaggcg gtcgatgtgt ctgtcctccg 5281 gaagggcccc aagcacgatg ttggtgccgg gcaaggtcgg cgggatgagg gccacgaacg 5341 ccagcacggc ctggggggtc atgctgccca taaggtaccg cgcggccggg tagcacagga 5401 gggcggcgat gggatggcgg tcgaagatga gggtgagggc cgggggcggg gcatgtgagc 5461 tcccagcctc ccccccgata tgaggagcca gaacggcgtc ggtcacggca taaggcatgc 5521 ccattgttat ctgggcgctt gtcattacca ccgccgcgtc cccggccgat atctcaccct 5581 ggtcgaggcg gtgttgtgtg gtgtagatgt tcgcgattgt ctcggaagcc cccagcaccc 5641 gccagtaagt catcggctcg ggtacgtaga cgatatcgtc gcgcgaaccc agggccacca 5701 gcagttgcgt ggtggtggtt ttccccatcc cgtggggacc gtctatataa acccgcagta 5761 gcgtgggcat tttctgctcc gggcggactt ccgtggcttc ttgctgccgg cgagggcgca 5821 acgccgtacg tcggttgcta tggccgcgag aacgcgcagc ctggtcgaac gcagacgcgt 5881 gttgatggcc ggggtacgaa gccatattac cctgttatcc ctagaattct ttgccaaaat 5941 gatgagacag cacaataacc agcacgttgc ccaggagctg taggaaaaag aagaaggcat 6001 gaacatggtt agcagaggct ctagagccgc cggtcacacg ccagaagccg aaccccgccc 6061 tgccccgtcc cccccgaagg cagccgtccc cctgcggcag ccccgaggct ggagatggag 6121 aaggggacgg cggcgcggcg acgcacgaag gccctccccg cccatttcct tcctgccggc 6181 gccgcaccgc ttcgcccgcg cccgctagag ggggtgcggc ggcgcctccc agatttcggc 6241 tccgccagat ttgggacaaa ggaagtccct gcgccctctc gcacgattac cataaaaggc 6301 aatggctgcg gctcgccgcg cctcgacagc cgccggcgct ccggggccgc cgcgcccctc 6361 ccccgagccc tccccggccc gaggcggccc cgccccgccc ggcaccccca cctgccgcca 6421 ccccccgccc ggcacggcga gccccgcgcc acgccccgca cggagccccg cacccgaagc 6481 cgggccgtgc tcagcaactc ggggaggggg gtgcaggggg gggttacagc ccgaccgccg 6541 cgcccacacc ccctgctcac ccccccacgc acacaccccg cacgcagcct ttgttcccct 6601 cgcagccccc ccgcaccgcg gggcaccgcc cccggccgcg ctcccctcgc gcacacgcgg 6661 agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag cgcgggccgc 6721 acgcggcgct ccccacgcac acacacacgc acgcaccccc cgagccgctc ccccccgcac 6781 aaagggccct cccggagccc tttaaggctt tcacgcagcc acagaaaaga aacgagccgt 6841 cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc gctcacctgt 6901 gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcgcg gagcggggca 6961 cggggcgaag gcaacgcagc gactcccgcc cgccgcgcgc ttcgcttttt atagggccgc 7021 cgccgccgcc gcctcgccat aaaaggaaac tttcggagcg cgccgctctg attggctgcc 7081 gccgcacctc tccgcctcgc cccgccccgc ccctcgcccc gccccgcccc gcctggcgcg 7141 cgcccccccc ccccccgccc ccatcgctgc acaaaataat taaaaaataa ataaatacaa 7201 aattgggggt ggggaggggg gggagatggg gagagtgaag cagaacgtgg ggctcacctc 7261 gacccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata 7321 aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg 7381 gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatagt 7441 ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta 7501 ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt 7561 atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt 7621 aataactagc gctgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga 7681 aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 7741 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 7801 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 7861 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 7921 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 7981 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 8041 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 8101 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta cgccttaaga 8161 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 8221 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 8281 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 8341 agcaagtaaa acctctacaa atgtggtatg gctgattatg atctagagtc gcggccgcta 8401 ttacgcacaa gagttccgta gctgttcaag tttgtgtttc aactgttctc gtcgtttccg 8461 caacaagtcc tcttcagaaa tgagcttttg ctcctctgct tggacggaca ggatgtatgc 8521 tgtggctttt ttaaggataa ctaccttggg ggccttttca ttgttttcca actccgggat 8581 ctggtcacgc agggcaaaaa agctccgttt tagctcgttc ctcctctggc gctccaagac 8641 gttgtgtgtt cgcctcttga cattctcctc ggtgtccgag gacctggggc tggtgcattt 8701 tcggttgttg ctgatctgtc tcaggactct gacactgtcc aacttgaccc tcttggcagc 8761 aggatagtcc ttccgagtgg agggaggcgc tgcgtagttg tgctgatgtg tggagacgtg 8821 gcacctcttg aggaccagtg ggctgtgagg aggtttgctg tggcctccag cagaaggtga 8881 tccagactct gaccttttgc caggagcctg cctcttttcc acagaaacaa catcgatttc 8941 ttcctcatct tcttgttcct cctcagagtc gctgctggtg gtgggcggtg tctcctcatg 9001 gagcaccagg ggctcggggc tgccctgcgg ggaggactcc gtcgaggaga gcagagaatc 9061 cgaggacgga gagaaggcgc tggagtcttg cgaggcgcag gacttgggcg agctgctgtc 9121 gttgagaggg taggggaaga ccaccgaggg gtcgatgcac tctgaggcgg cggcgctcag 9181 atcctgcagg tacaagctgg aggtggagca gacgctgtgg ccgcgggcgg ggttcgggct 9241 gccgctgtct ttgcgcgcag cctggtagga ggccagcttc tctgagacga gcttggcggc 9301 ggccgagaag ccgctccaca tacagtcctg gatgatgatg tttttgatga aggtctcgtc 9361 gtccgggtcg cagatgaaac tctggttcac catgtctcct cccagcagct cggtcaccat 9421 ctccagctgg tcggccgtgg agaagctccc gccaccgccg tcgttgtctc cccgaaggga 9481 gaagggtgtg accgcaacgt aggagggcga gcagagcccg gagcggcggc taggggacag 9541 gggcggggtg ggcagcagct cgaatttctt ccagatatcc tcgctgggcg ccgggggctg 9601 cagctcgctc tgctgctgct gctggtagaa gttctcctcc tcgtcgcagt agaaatacgg 9661 ctgcaccgag tcgtagtcga ggtcatagtt cctgttggtg aagctaacgt tgaggggcat 9721 aggaccgggg ttactttcaa catcgccagc gagtttcaac aaagcgtagt tagtacattg 9781 cccactaccc atgtgtgaga ggggcagtgt gccgttaatg gccgtgccgg gcaccgggcc 9841 gctctggtag tgctgggaca tgtgaagtct gctgggggcg gcgggttccg gcacctcggc 9901 gccggggaga tacatgctga tcatgtcccg gaggtccccg gcctggcagg gcgccctgga 9961 gtgggaggaa gaggtaacca caggggggct ggagctggcc tcggacttga ccaccgaacc 10021 catggagcca agagccatgc caggggtgcc ctgctgcgag taggacatgc tgtaggtggg 10081 cgagccgttc atgtaggtct gcgagctggt catggagttg tactgcaggg cgctcacgtc 10141 gtagcggtgc atgggctgca tctgcgctgc gccgtgcgca ttgaggcccg ggtgctgcgg 10201 gtagcccagc tggtcctgca tcatgctgta gctgccgttg ctccagccgt tcatgtgcgc 10261 gtaactgtcc atgcgctggt tcacgcccgc gcccaggccg gcgcccaccc cgaccccgct 10321 cgccatgcta ttgccgccgg gggccagcag cccgccgggc agcgtgtact tatccttctt 10381 catgagcgtc ttggttttcc gccggggccg gtatttataa tccgggtgct ccttcatgtg 10441 cagcgctcgc agccgcttag cctcgtcgat gaacggccgc ttctccgtct ccgacaaaag 10501 tttccactcg gcgcccaggc gcttgctgat ctccgagttg tgcatcttgg ggttctcctg 10561 ggccatcttg cgccgctgcc cgcgggacca caccatgaag gcattcatgg gccgcttgac 10621 gcggtccggg ctgtttttct ggttgccgcc ggccgccgcc gcggtggagt tgccgccgcc 10681 gccccccgaa gtttgctgcg ggcccggcgg cttcagctcc gtctccatca tgttgtacat 10741 ggggccgggg ttctcctcca cgtcgccgca ggtcagcagg ctgcccctgc cctcgccgct 10801 gccaaaatgc ctcttcatgt gtaaggcgag gtggtccgac ctggaaaatg ctcggtcgca 10861 tttttggcac tggaacgggc ggtgccccgt gtgtttacgg tagtgcctgg tcagttcatc 10921 tgagcgggcg aatttccatc cacagccgtc ccagtcacag tggtaaggtt tctcacctgt 10981 gtgggttcgc aggtgtgcct tgagatggga actctttgtg taggttttgc cgcagcccgc 11041 gtaatcacaa gtgtgggtgg cggtcctttt ccggggccac gatcgtcttc ccctctttgg 11101 cttgggctcc tctggcatgc aggaaccggg tggcatgagc tcttggtaat ggagcggcgg 11161 gacttgcggc tgcatctgat cgggcaggaa ggatgggtaa ttgggccccg ggtggggatg 11221 gaagccggga ggaagcggca gggcagggtg acagtccctg ctgctcagca cttcctcaag 11281 acccagggtc ggggtagtcc tgctggggag ctgccgcccc agggggaagt cgtgtgcagc 11341 cggccggtgg ccattgctga gagggggtcc agcgcccaag tgggtgcacg aagagaccgc 11401 ctcctgcttg atcttggggc acgtgcgcgg cggcccgccg ttgtagggcg ccaccaccac 11461 cgggtggctg ccgtcagggc tgcctttgct gacgctgatg accgacgggc tgccgtactc 11521 gctgccaggg gcgctcagcg acgccttcag cacgaacttg cccatcagcc cgccacctgg 11581 cggctgcggc tgctgcggcg gaatgtacac cgggtccaat tctggccgca ggagctcggc 11641 cacgaagccg cccgaggggc tcacgtcgtt gatgtccgcc aggttgaagg gagccgtcgg 11701 agggggagcg gactccctgc catagaggag gcctccgccc gtgccgcccg gcgccacgcc 11761 cgggtcgttc ccggcccgga tcggataggt gaagctgcag gtggagggcg cgctggcagg 11821 gccgctgctc gacggcgacg acgaagagga ggctgacgct gacgaggaca cggtggcggc 11881 cactgactcc ggaggatggg tcagcgaatt ggagagaata aagtccaggt ccaggagatc 11941 gttgaactcc tcggtctctc tccgaggtag gggcgccagg ttgctaccgc cgcaagccgc 12001 accggctccg ccgctctcca ggtctgtggc cacggtcgcc gccgccaggt cataggggcg 12061 gccgggaagc actgggggaa gtcgcttcat gtgggagagc tcctcccgcc agcggttatt 12121 cggggcacct gcttgacgca gtgtcttctc ccttcccgcc gggccagacg cgaacgtgga 12181 gaaagatggg agcagcgcgt cgctgacagc catgggccct gggttggact ccacgtctcc 12241 cgccaacttg agaaggtcaa aattcaaagt ctgtttcacg ccacttccgt ttgaatgcat 12301 gggagagccc agagtggtga cggagacagg gggaaaggct tccccctcag ggaaagggac 12361 cgaggagtac agtgcagtga agtgagggct cccatagcct ggggtaccaa aatggggccc 12421 tggggccaga ggaaaggaca ctggtccccc tgagaaagga gacccagcag cctcaaaatc 12481 ctctcgttgt gcatagtcgc tgcttgatcg cttgcccttc tggcgccggt tacagaacca 12541 cactcggacc acatccttct cgagcccaag ctgctgggcg atgtggctga tctgctgcag 12601 tgtgggtttc gggcactgca ggaacaaatt ctccaggttg cctctcactc ggttctcgat 12661 actggttcgc tttctctttc gggcctgcac gagggtttct gctttgcata tctcctgaag 12721 attttcattg ttgtcagctt cctccaccca cttctgcagc aagggccgca gcttacacat 12781 gttcttgaag ctaagctgca gagcctcaaa gcggcagatg gtcgtttggc tgaatacctt 12841 cccaaataga acccccaggg tgagccccac atcggcctgt gtatatccca gggtgatcct 12901 cttctgcttc aggagcttgg caaattgctc gagttctttc tgcagagctt tgatgtcctg 12961 ggactcctcc gggttttgct ccagcttctc cttctccagc ttcacggcac caggggtgac 13021 ggtgcagggc tccggggagg ccccatcgga gttgctctcc accccgactc ctgcttcgcc 13081 ctcaggctga gaggtctcca agccgccttg gggcactagc cccactccaa cctggggccc 13141 acagtacgcc atccccccac agaactcata cggcgggggg catgggggaa tcccccacac 13201 ctcagagcct ggcccaaccc ccggcccgat tcctggccct ccaggagggc cttggaagct 13261 tagccaggtc cgaggatcaa cccagcccgg ctccggcccc cctggcccat cacctccacc 13321 acctggaggg ggcgagaagg cgaaatccga agccaggtgt cccgccatgg tggcgctagc 13381 ttcacgacac ctgaaatgga agaaaaaaac tttgaaccac tgtctgaggc ttgagaatga 13441 accaagatcc aaactcaaaa agggcaaatt ccaaggagaa ttacatcaag tgccaagctg 13501 gcctaacttc agtctccacc cactcagtgt ggggaaactc catcgcataa aacccctccc 13561 cccaacctaa agacgacgta ctccaaaagc tcgagaacta atcgaggtgc ctggacggcg 13621 cccggtactc cgtggagtca catgaagcga cggctgagga cggaaaggcc cttttccttt 13681 gtgtgggtga ctcacccgcc cgctctcccg agcgccgcgt cctccatttt gagctccctg 13741 cagcagggcc gggaagcggc catctttccg ctcacgcaac tggtgccgac cgggccagcc 13801 ttgccgccca gggcggggcg atacacggcg gcgcgaggcc aggcaccaga gcaggccggc 13861 cagcttgaga ctacccccgt ccgattctcg gtggccgcgc tcgcaggccc cgcctcgccg 13921 aacatgtgcg ctgggacgca cgggccccgt cgccgcccgc ggccccaaaa accgaaatac 13981 cagtgtgcag atcttggccc gcatttacaa gactatcttg ccagaaaaaa agcgtcgcag 14041 caggtcatca aaaattttaa atggctagag acttatcgaa agcagcgaga caggcgcgaa 14101 ggtgccacca gattcgcacg cggcggcccc agcgcccagg ccaggcctca actcaagcac 14161 gaggcgaagg ggctccttaa gcgcaaggcc tcgaactctc ccacccactt ccaacccgaa 14221 gctcgggatc aagaatcacg tactgcagcc aggtggaagt aattcaaggc acgcaagggc 14281 cataacccgt aaagaggcca ggcccgcggg aaccacacac ggcacttacc tgtgttctgg 14341 cggcaaaccc gttgcgaaaa agaacgttca cggcgactac tgcacttata tacggttctc 14401 ccccaccctc gggaaaaagg cggagccagt acacgacatc actttcccag tttaccccgc 14461 gccaccttct ctaggcaccg gttcaattgc cgacccctcc ccccaacttc tcggggactg 14521 tgggcgatgt gcgctctgcc cactgacggg caccggagcc tcacgattaa ttaataggga 14581 taacagggta atttaaatag atctactagt tattaatagt aatcaattac ggggtcatta 14641 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc 14701 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg 14761 ccaataggga ctttccattg acgtcaatgg gtggactatt tacggtaaac tgcccacttg 14821 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa 14881 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac 14941 atctacgtat tagtcatcgc tattaccatg ggtcgaggtg agccccacgt tctgcttcac 15001 tctccccatc tcccccccct ccccaccccc aattttgtat ttatttattt tttaattatt 15061 ttgtgcagcg atgggggcgg gggggggggg ggcgcgcgcc aggcggggcg gggcggggcg 15121 aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc ggcgcgctcc 15181 gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc 15241 ggcgggcggg agtcgctgcg ttgccttcgc cccgtgcccc gctccgcgcc gcctcgcgcc 15301 gcccgccccg gctctgactg accgcgttac tcccacaggt gagcgggcgg gacggccctt 15361 ctcctccggg ctgtaattag cgcttggttt aatgacggct cgtttctttt ctgtggctgc 15421 gtgaaagcct taaagggctc cgggagggcc ctttgtgcgg gggggagcgg ctcggggggt 15481 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggcc cgcgctgccc ggcggctgtg 15541 agcgctgcgg gcgcggcgcg gggctttgtg cgctccgcgt gtgcgcgagg ggagcgcggc 15601 cgggggcggt gccccgcggt gcgggggggc tgcgagggga acaaaggctg cgtgcggggt 15661 gtgtgcgtgg gggggtgagc agggggtgtg ggcgcggcgg tcgggctgta acccccccct 15721 gcacccccct ccccgagttg ctgagcacgg cccggcttcg ggtgcggggc tccgtgcggg 15781 gcgtggcgcg gggctcgccg tgccgggcgg ggggtggcgg caggtggggg tgccgggcgg 15841 ggcggggccg cctcgggccg gggagggctc gggggagggg cgcggcggcc ccggagcgcc 15901 ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc gtgcgagagg 15961 gcgcagggac ttcctttgtc ccaaatctgg cggagccgaa atctgggagg cgccgccgca 16021 ccccctctag cgggcgcggg cgaagcggtg cggcgccggc aggaaggaaa tgggcgggga 16081 gggccttcgt gcgtcgccgc gccgccgtcc ccttctccat ctccagcctc ggggctgccg 16141 cagggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg 16201 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc 16261 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt cgctagtcga 16321 cgagcggccg cagctctaaa tactctgaag tccaaagaag ttgtatgttg ggtgggctcc 16381 cttcaacttt aacatggaag tgctttctgt gactttaaaa gtaagtgctt ccatgtttta 16441 gtaggagtga atccaattta cttctccaaa atagaacacg ctaacctcat ttgaagggat 16501 cccctttgct ttaacatggg ggtacctgct gtgtgaaaca aaagtaagtg cttccatgtt 16561 tcagtggagg tgtctccaag ccagcacacc ttttgttaca aaattttttt gttattgtgt 16621 tttaaggtta ctaagcttgt tacaggttaa aggattctaa ctttttccaa gactgggctc 16681 cccaccactt aaacgtggat gtacttgctt tgaaactaaa gaagtaagtg cttccatgtt 16741 ttggtgatgg taagtcttcc ttttacattt ttattatttt tttagaaaat aactttattg 16801 tattgaccgc agctcatata tttaagcttt attttgtatt tttacatctg ttaaggggcc 16861 ccctctactt taacatggag gcacttgctg tgacatgaca aaaataagtg cttccatgtt 16921 tgagtgtggt ggttcctacc taatcagcaa ttgcgttaac gcccacactg tgtgcagttc 16981 ttggctacag gccattactg ttgctaatat gcaactctgt tgaatataaa ttggaattgc 17041 actttagcaa tggtgatgga ttgttaagcc aatgacagaa tttaaaccac agacttactt 17101 tgatagcact cttaatggta taacttcttc tcccaggcgc gtctagatca taatcagcca 17161 taccacattg tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg 17221 aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac 17281 aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt 17341 tgtggtttgt ccaaactcat caatgtatct taaagacgtc tttaattaat cgactctaga 17401 ggatcgatgc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg 17461 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc 17521 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct 17581 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc 17641 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca 17701 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa 17761 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa 17821 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta 17881 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat 17941 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga 18001 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt 18061 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg 18121 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg 18181 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg 18241 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa 18301 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt 18361 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac 18421 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga 18481 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca 18541 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa 18601 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca 18661 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact 18721 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat 18781 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct 18841 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt 18901 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata 18961 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat 19021 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat 19081 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat 19141 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat 19201 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac 19261 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta 19321 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta 19381 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta 19441 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta 19501 atctgtatcc gggtagcata tgctatcctc atgcatatac agtcagcata tgatacccag 19561 tagtagagtg ggagtgctat cctttgcata tgccgccacc tcccaagggg gcgtgaattt 19621 tcgctgcttg tccttttcct gctggttgct cccattctta ggtgaattta aggaggccag 19681 gctaaagccg tcgcatgtct gattgctcac caggtaaatg tcgctaatgt tttccaacgc 19741 gagaaggtgt tgagcgcgga gctgagtgac gtgacaacat gggtatgccc aattgcccca 19801 tgttgggagg acgaaaatgg tgacaagaca gatggccaga aatacaccaa cagcacgcat 19861 gatgtctact ggggatttat tctttagtgc gggggaatac acggctttta atacgattga 19921 gggcgtctcc taacaagtta catcactcct gcccttcctc accctcatct ccatcacctc 19981 cttcatctcc gtcatctccg tcatcaccct ccgcggcagc cccttccacc ataggtggaa 20041 accagggagg caaatctact ccatcgtcaa agctgcacac agtcaccctg atattgcagg 20101 taggagcggg ctttgtcata acaaggtcct taatcgcatc cttcaaaacc tcagcaaata 20161 tatgagtttg taaaaagacc atgaaataac agacaatgga ctcccttagc gggccaggtt 20221 gtgggccggg tccaggggcc attccaaagg ggagacgact caatggtgta agacgacatt 20281 gtggaatagc aagggcagtt cctcgcctta ggttgtaaag ggaggtctta ctacctccat 20341 atacgaacac accggcgacc caagttcctt cgtcggtagt cctttctacg tgactcctag 20401 ccaggagagc tcttaaacct tctgcaatgt tctcaaattt cgggttggaa cctccttgac 20461 cacgatgctt tccaaaccac cctccttttt tgcgcctgcc tccatcaccc tgaccccggg 20521 gtccagtgct tgggccttct cctgggtcat ctgcggggcc ctgctctatc gctcccgggg 20581 gcacgtcagg ctcaccatct gggccacctt cttggtggta ttcaaaataa tcggcttccc 20641 ctacagggtg gaaaaatggc cttctacctg gagggggcct gcgcggtgga gacccggatg 20701 atgatgactg actactggga ctcctgggcc tcttttctcc acgtccacga cctctccccc 20761 tggctctttc acgacttccc cccctggctc tttcacgtcc tctaccccgg cggcctccac 20821 tacctcctcg accccggcct ccactacctc ctcgaccccg gcctccactg cctcctcgac 20881 cccggcctcc acctcctgct cctgcccctc ctgctcctgc ccctcctcct gctcctgccc 20941 ctcctgcccc tcctgctcct gcccctcctg cccctcctgc tcctgcccct cctgcccctc 21001 ctgctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgcccct cctcctgctc 21061 ctgcccctcc tgcccctcct gctcctgccc ctcctgcccc tcctgctcct gcccctcctg 21121 cccctcctgc tcctgcccct cctgctcctg cccctcctgc tcctgcccct cctgctcctg 21181 cccctcctgc ccctcctgcc cctcctcctg ctcctgcccc tcctgctcct gcccctcctg 21241 cccctcctgc ccctcctgct cctgcccctc ctcctgctcc tgcccctcct gcccctcctg 21301 cccctcctcc tgctcctgcc cctcctgccc ctcctcctgc tcctgcccct cctcctgctc 21361 ctgcccctcc tgcccctcct gcccctcctc ctgctcctgc ccctcctgcc cctcctcctg 21421 ctcctgcccc tcctcctgct cctgcccctc ctgcccctcc tgcccctcct cctgctcctg 21481 cccctcctcc tgctcctgcc cctcctgccc ctcctgcccc tcctgcccct cctcctgctc 21541 ctgcccctcc tcctgctcct gcccctcctg ctcctgcccc tcccgctcct gctcctgctc 21601 ctgttccacc gtgggtccct ttgcagccaa tgcaacttgg acgtttttgg ggtctccgga 21661 caccatctct atgtcttggc cctgatcctg agccgcccgg ggctcctggt cttccgcctc 21721 ctcgtcctcg tcctcttccc cgtcctcgtc catggttatc accccctctt ctttgaggtc 21781 cactgccgcc ggagccttct ggtccagatg tgtctccctt ctctcctagg ccatttccag 21841 gtcctgtacc tggcccctcg tcagacatga ttcacactaa aagagatcaa tagacatctt 21901 tattagacga cgctcagtga atacagggag tgcagactcc tgccccctcc aacagccccc 21961 ccaccctcat ccccttcatg gtcgctgtca gacagatcca ggtctgaaaa ttccccatcc 22021 tccgaaccat cctcgtcctc atcaccaatt actcgcagcc cggaaaactc ccgctgaaca 22081 tcctcaagat ttgcgtcctg agcctcaagc caggcctcaa attcctcgtc cccctttttg 22141 ctggacggta gggatgggga ttctcgggac ccctcctctt cctcttcaag gtcaccagac 22201 agagatgcta ctggggcaac ggaagaaaag ctgggtgcgg cctgtgagga tcagcttatc 22261 gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg atacgcctat 22321 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg 22381 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc 22441 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta 22501 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg 22561 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg 22621 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac 22681 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg 22741 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt 22801 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg 22861 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac 22921 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt 22981 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag 23041 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc 23101 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc 23161 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta 23221 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg 23281 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga 23341 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac 23401 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa 23461 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat 23521 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc 23581 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg 23641 gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc 23701 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg 23761 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg 23821 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa 23881 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg 23941 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga 24001 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct 24061 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca 24121 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttgaagctgt ccctgatggt 24181 cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg ccgccggaag 24241 cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgagcgat taaacgcgcc 24301 aactagagga tcgagcccca gctggttctt tccgcctcag aagccataga gcccaccgca 24361 tccccagcat gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca 24421 ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag 24481 gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca 24541 acagatggct ggcaactaga aggcacagtc gaggctgatc agcgagctcg tttatcttat 24601 caattctgtg cctccgggag cagggtaggg ctgtggattt cttcttcttc ctctcgaaag 24661 taggttggct ttccctgtgc actagggccc tgctgggcct tcagcggaca tgaggctacc 24721 atatggctga tgctctggca gaagtggcac ttcttgggct ggggtggcag cttgcattcc 24781 ttggcatgat gatctagacc tccacagttg tagcacctgt ctccttttga tctgcgcttc 24841 tgcatgctct ttccttttgg ccgcctctca ctcccaatac agaatactcc accaggtccg 24901 gtgacacgga tggattccag acccttggct gacttcttaa aggtgaactc cactgcctca 24961 ccctccttca agctccggaa cccttccatg tgcagcttac tctggtgcac aaagacatcc 25021 actggggggt cgagcgcgac cccggcgcgg gcggtcatgg acaggaagcc gaaccccatg 25081 cgcacgttga accacttaca gatgcccgca ccgtgcagca gctgaggctc gtccgccgcc 25141 cgggccgcgt cctccggcgc ctcctcgggc gcctcttctg ccgccttggc gcagccacct 25201 gcaaactgct ggttggacac ggagcccatg ggccctgggt tggactccac gtctcccgcc 25261 aacttgagaa ggtcaaaatt caaagtctgt ttcacgccac ttcccacgtc ttcaggttgc 25321 atgttcatgg agtagtttag gaataaatcc atggtttgtg gagtactaaa atacctagtg 25381 gtctgctgta ttacattaag gccttcccca gcagcttcca aggcagcctc caagtcactg 25441 gcaggagaat ttggctggaa ctgcatgcag gactgcagag attcctctcc acagttatag 25501 aagggactgt tccaggcctg attgttccag gattgggtgc accaggtctg agtgttccag 25561 gagtggttgc tccaggactg gatgttctgg gtctggttgc tccaggttga attgttccag 25621 gtctggttgc tccacattgg aaggttccca gtcgggttca ccaggcatcc ctggtggtag 25681 gaagagtaaa ggctggggta ggtaggtgct gaggccttct gcgtcacacc attgctattc 25741 ttcggccagt tgtttttctg ccacctctta gatttcattc tctggttctg gaaccaggtc 25801 ttcacctgtt tgtagctgag gttcaggatg ttggagagtt cttgcatctg ctggaggctg 25861 aggtatttct gtctctgaaa tctatcattg agtacacaca gctgggtgga agagaacaca 25921 gttctggtct tctgtttctt gaccgggacc ttgtcttcct tttttgcgac actcttctct 25981 gcagaagtgg gttgtttgcc tttgggactg gtggaagaat cagggctgtc ctgaataagc 26041 agatccatgg aggaaggaag aggagagaca gtctccgtgt gaggcatctc agcagaagac 26101 atttgcaagg atggatagtt ttcttcaggc ccacaaatca caggcatagg tgaagattct 26161 ttacagtcgg atgcttcaaa gcaaggcaag ctttggggac aagctggatc cacactcatg 26221 gtggcttaat cgcgaattct ttgccaaaat gatgagacag cacaataacc agcacgttgc 26281 ccaggagctg taggaaaaag aagaaggcat gaacatggtt agcagaggct ctagagccgc 26341 cggtcacacg ccagaagccg aaccccgccc tgccccgtcc cccccgaagg cagccgtccc 26401 cctgcggcag ccccgaggct ggagatggag aaggggacgg cggcgcggcg acgcacgaag 26461 gccctccccg cccatttcct tcctgccggc gccgcaccgc ttcgcccgcg cccgctagag 26521 ggggtgcggc ggcgcctccc agatttcggc tccgccagat ttgggacaaa ggaagtccct 26581 gcgccctctc gcacgattac cataaaaggc aatggctgcg gctcgccgcg cctcgacagc 26641 cgccggcgct ccggggccgc cgcgcccctc ccccgagccc tccccggccc gaggcggccc 26701 cgccccgccc ggcaccccca cctgccgcca ccccccgccc ggcacggcga gccccgcgcc 26761 acgccccgca cggagccccg cacccgaagc cgggccgtgc tcagcaactc ggggaggggg 26821 gtgcaggggg gggttacagc ccgaccgccg cgcccacacc ccctgctcac ccccccacgc 26881 acacaccccg cacgcagcct ttgttcccct cgcagccccc ccgcaccgcg gggcaccgcc 26941 cccggccgcg ctcccctcgc gcacacgcgg agcgcacaaa gccccgcgcc gcgcccgcag 27001 cgctcacagc cgccgggcag cgcgggccgc acgcggcgct ccccacgcac acacacacgc 27061 acgcaccccc cgagccgctc ccccccgcac aaagggccct cccggagccc tttaaggctt 27121 tcacgcagcc acagaaaaga aacgagccgt cattaaacca agcgctaatt acagcccgga 27181 ggagaagggc cgtcccgccc gctcacctgt gggagtaacg cggtcagtca gagccggggc 27241 gggcggcgcg aggcggcgcg gagcggggca cggggcgaag gcaacgcagc gactcccgcc 27301 cgccgcgcgc ttcgcttttt atagggccgc cgccgccgcc gcctcgccat aaaaggaaac 27361 tttcggagcg cgccgctctg attggctgcc gccgcacctc tccgcctcgc cccgccccgc 27421 ccctcgcccc gccccgcccc gcctggcgcg cgcccccccc ccccccgccc ccatcgctgc 27481 acaaaataat taaaaaataa ataaatacaa aattgggggt ggggaggggg gggagatggg 27541 gagagtgaag cagaacgtgg ggctcacctc gacccatggt aatagcgatg actaatacgt 27601 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg 27661 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta 27721 ctgccaagtg ggcagtttac cgtaaatagt ccacccattg acgtcaatgg aaagtcccta 27781 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg 27841 gtcagccagg cgggccattt accgtaagtt atgtaacgc //