质粒名称: | SaABE8e |
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出品公司: | Addgene |
目录编号: | 138500 |
存储实验室: | David Liu |
载体骨架基本信息 |
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载体名称: | pCMV with a BR322 origin |
载体出品公司: | Clontech |
完整质粒大小: | 7275bp |
载体类型: | 哺乳细胞表达 |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 未知 |
插入基因信息 |
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插入基因名称: | iRFP713 |
物种来源: | 光合细菌 Rhodopseudomonas palustris |
插入大小: | 951 bp |
基因突变: | T2A, S13L, A92T, V104T, V114I, E161K, Y193K, F198Y, D202T, I203V, Y258F, A283V, K288T, N290Y relative to RpBphP2 |
GenBank编号 | JN247409 |
启动子: | CMV |
标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII(N端) |
克隆方法: | 限制性内切酶 |
5’克隆位点: | AgeI(未破坏) |
3’克隆位点: | NotI (未破坏) |
5’测序引物 | CMV-F |
3’测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | |
参考文献部分: |
买家导航
SaABE8e载体质粒基本信息
SaABE8e质粒图谱载体图谱和SaABE8e载体序列质粒序列多克隆位点信息
SaABE8e质粒载体简介
SaABE8e质粒序列载体序列
LOCUS SaABE8e 7275 bp DNA circular SYN 25-AUG-2024 DEFINITION A-to-G base editor. ACCESSION . VERSION . KEYWORDS . SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 7275) AUTHORS Richter MF, Zhao KT, Eton E, Lapinaite A, Newby GA, Thuronyi BW, Wilson C, Koblan LW, Zeng J, Bauer DE, Doudna JA, Liu DR TITLE Phage-assisted evolution of an adenine base editor with improved Cas domain compatibility and activity. JOURNAL Nat Biotechnol. 2020 Jul;38(7):883-891. doi: 10.1038/s41587-020-0453-z. Epub 2020 Mar 16. PUBMED 32433547 REFERENCE 2 (bases 1 to 7275) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 25, 2024 from SnapGene Server 7.0.3 https://www.biofeng.com COMMENT SGRef: number: 1; type: "Journal Article"; doi: "10.1038/s41587-020-0453-z"; journalName: "Nat Biotechnol"; date: "2020-07"; volume: "38"; issue: "7"; pages: "883-891" FEATURES Location/Qualifiers source 1..7275 /mol_type="other DNA" /organism="synthetic DNA construct" promoter 132..335 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 286..306 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 377..396 /label=T7 /note="T7 promoter, forward primer" promoter 377..395 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 445..465 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4261..4281 /codon_start=1 /product="nuclear localization signal of SV40 (simian virus 40) large T antigen" /label=SV40 NLS /translation="PKKKRKV" CDS 4290..4307 /codon_start=1 /product="6xHis affinity tag" /label=6xHis /translation="HHHHHH" primer_bind complement(4330..4347) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 4336..4560 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" primer_bind complement(4631..4647) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(4631..4647) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(4644..4666) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 4655..4671 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(4679..4709) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 4724..4745 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(4862..4879) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(5033..5621) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(5113..5132) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5792..6652) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 6415..6434 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6653..6757) /gene="bla" /label=AmpR promoter primer_bind complement(6836..6855) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" enhancer join(7027..7275,1..131) /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" ORIGIN 1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg 61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg 121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact 181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa 241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta 301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct 361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca 421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga ggtggagttt 481 tcccacgagt actggatgag acatgccctg accctggcca agagggcacg ggatgagagg 541 gaggtgcctg tgggagccgt gctggtgctg aacaatagag tgatcggcga gggctggaac 601 agagccatcg gcctgcacga cccaacagcc catgccgaaa ttatggccct gagacagggc 661 ggcctggtca tgcagaacta cagactgatt gacgccaccc tgtacgtgac attcgagcct 721 tgcgtgatgt gcgccggcgc catgatccac tctaggatcg gccgcgtggt gtttggcgtg 781 aggaactcaa aaagaggcgc cgcaggctcc ctgatgaacg tgctgaacta ccccggcatg 841 aatcaccgcg tcgaaattac cgagggaatc ctggcagatg aatgtgccgc cctgctgtgc 901 gatttctatc ggatgcctag acaggtgttc aatgctcaga agaaggccca gagctccatc 961 aactccggag gatctagcgg aggctcctct ggctctgaga cacctggcac aagcgagagc 1021 gcaacacctg aaagcagcgg gggcagcagc ggggggtcag ggaagcgaaa ttacattctg 1081 gggctggcca ttggcattac atcagtgggc tatggcatca ttgactacga gacaagggac 1141 gtgatcgacg ccggcgtgag actgttcaag gaggccaacg tggagaacaa tgagggccgg 1201 agatccaaga ggggagcaag gcgcctgaag cggagaaggc gccacagaat ccagagagtg 1261 aagaagctgc tgttcgatta caacctgctg accgaccact ccgagctgtc tggcatcaat 1321 ccttatgagg ccagagtgaa gggcctgtcc cagaagctgt ctgaggagga gtttagcgcc 1381 gccctgctgc acctggcaaa gaggagaggc gtgcacaacg tgaatgaggt ggaggaggac 1441 accggcaacg agctgtccac aaaggagcag atcagccgca attccaaggc cctggaggag 1501 aagtatgtgg ccgagctgca gctggagcgg ctgaagaagg atggcgaggt gaggggctcc 1561 atcaatcgct tcaagacctc tgactacgtg aaggaggcca agcagctgct gaaggtgcag 1621 aaggcctacc accagctgga tcagtccttt atcgatacat atatcgacct gctggagaca 1681 aggcgcacat actatgaggg accaggagag ggctctccct tcggctggaa ggacatcaag 1741 gagtggtacg agatgctgat gggccactgc acctattttc cagaggagct gagaagcgtg 1801 aagtacgcct ataacgccga tctgtacaac gccctgaatg acctgaacaa cctggtcatc 1861 accagggatg agaacgagaa gctggagtac tatgagaagt tccagatcat cgagaacgtg 1921 ttcaagcaga agaagaagcc tacactgaag cagatcgcca aggagatcct ggtgaacgag 1981 gaggacatca agggctaccg cgtgacctcc acaggcaagc cagagttcac caatctgaag 2041 gtgtatcacg atatcaagga catcacagcc cggaaggaga tcatcgagaa cgccgagctg 2101 ctggatcaga tcgccaagat cctgaccatc tatcagagct ccgaggacat ccaggaggag 2161 ctgaccaacc tgaatagcga gctgacacag gaggagatcg agcagatcag caatctgaag 2221 ggctacaccg gcacacacaa cctgagcctg aaggccatca atctgatcct ggatgagctg 2281 tggcacacaa acgacaatca gatcgccatc tttaaccggc tgaagctggt gccaaagaag 2341 gtggacctgt cccagcagaa ggagatccca accacactgg tggacgattt catcctgtct 2401 cccgtggtga agcggagctt catccagagc atcaaagtga tcaacgccat catcaagaag 2461 tacggcctgc ccaatgatat catcatcgag ctggccaggg agaagaactc caaggacgcc 2521 cagaagatga tcaatgagat gcagaagagg aaccgccaga ccaatgagcg gatcgaggag 2581 atcatcagaa ccacaggcaa ggagaacgcc aagtacctga tcgagaagat caagctgcac 2641 gatatgcagg agggcaagtg tctgtattct ctggaggcca tccctctgga ggacctgctg 2701 aacaatccat tcaactacga ggtggatcac atcatccccc ggagcgtgag cttcgacaat 2761 tcttttaaca ataaggtgct ggtgaagcag gaggagaaca gcaagaaggg caataggacc 2821 cctttccagt acctgtctag ctccgattct aagatcagct acgagacatt caagaagcac 2881 atcctgaatc tggccaaggg caagggccgc atcagcaaga ccaagaagga gtacctgctg 2941 gaggagcggg acatcaacag attctccgtg cagaaggact tcatcaaccg gaatctggtg 3001 gacaccagat acgccacacg cggcctgatg aatctgctgc ggtcttattt cagagtgaac 3061 aatctggatg tgaaggtgaa gagcatcaac ggcggcttca cctcctttct gcggagaaag 3121 tggaagttta agaaggagcg caacaagggc tataagcacc acgccgagga tgccctgatc 3181 atcgccaatg ccgacttcat ctttaaggag tggaagaagc tggacaaggc caagaaagtg 3241 atggagaacc agatgttcga ggagaagcag gccgagagca tgcccgagat cgagacagag 3301 caggagtaca aggagatttt catcacacct caccagatca agcacatcaa ggacttcaag 3361 gactacaagt attctcacag ggtggataag aagcccaacc gcgagctgat caatgacacc 3421 ctgtatagca cacggaagga cgataagggc aataccctga tcgtgaacaa tctgaacggc 3481 ctgtacgaca aggataatga caagctgaag aagctgatca acaagtctcc cgagaagctg 3541 ctgatgtacc accacgatcc tcagacatat cagaagctga agctgatcat ggagcagtac 3601 ggcgacgaga agaacccact gtataagtac tatgaggaga caggcaacta cctgacaaag 3661 tatagcaaga aggataatgg ccccgtgatc aagaagatca agtactatgg caacaagctg 3721 aatgcccacc tggacatcac cgacgattac cctaactctc gcaataaggt ggtgaagctg 3781 agcctgaagc cataccggtt cgacgtgtac ctggacaacg gcgtgtataa gtttgtgaca 3841 gtgaagaatc tggatgtgat caagaaggag aactactatg aggtgaacag caagtgctac 3901 gaggaggcca agaagctgaa gaagatcagc aaccaggccg agttcatcgc ctctttttac 3961 aacaatgacc tgatcaagat caatggcgag ctgtatagag tgatcggcgt gaacaatgat 4021 ctgctgaaca gaatcgaagt gaatatgatc gacatcacct acagggagta tctggagaac 4081 atgaatgata agaggccccc tcgcatcatc aagaccatcg cctctaagac acagagcatc 4141 aagaagtaca gcacagacat cctggggaac ctgtatgaag tcaagagcaa gaaacatcct 4201 cagattatca agaaaggctc tggcggctca aaaagaaccg ccgacggcag cgaattcgag 4261 cccaagaaga agaggaaagt ctaaccggtc atcatcacca tcaccattga gtttaaaccc 4321 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg 4381 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa 4441 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca 4501 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg 4561 cttctgaggc ggaaagaacc agctggggct cgataccgtc gacctctagc tagagcttgg 4621 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 4681 acatacgagc cggaagcata aagtgtaaag cctagggtgc ctaatgagtg agctaactca 4741 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 4801 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 4861 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 4921 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 4981 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 5041 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 5101 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 5161 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 5221 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 5281 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 5341 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 5401 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 5461 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 5521 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 5581 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 5641 ctacggggtc tgacactcag tggaacgaaa actcacgtta agggattttg gtcatgagat 5701 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 5761 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 5821 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 5881 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 5941 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 6001 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 6061 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 6121 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 6181 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 6241 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 6301 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 6361 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 6421 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 6481 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 6541 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 6601 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 6661 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 6721 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 6781 ctgacgtcga cggatcggga gatcgatctc ccgatcccct agggtcgact ctcagtacaa 6841 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg 6901 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca 6961 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata 7021 cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc 7081 atagcccata tattgagttc cgcgttacat aacttacggt aaatggcccg cctggctgac 7141 cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa 7201 tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag 7261 tacatcaagt gtatc //
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