| 质粒名称: | pY010 (pcDNA3.1-hAsCpf1) |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 69982 |
| 存储实验室: | Feng Zhang |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5354 bp |
| 完整质粒大小: | 9464 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | hAsCpf1 |
| 物种来源: | Acidaminococcus_sp_BV3L6 |
| 插入大小: | 3921 bp |
| 启动子 | CMV |
| 标签/融合蛋白: | NLS-3xHA（C端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | KpnI(未破坏) |
| 3' 克隆位点: | EcoRI (未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pY010 (pcDNA3.1-hAsCpf1) was a gift from Feng Zhang (Addgene plasmid # 69982) |
| 参考文献部分: | Cpf1 Is a Single RNA-Guided Endonuclease of a Class 2 CRISPR-Cas System. Zetsche B, Gootenberg JS, Abudayyeh OO, Slaymaker IM, Makarova KS, Essletzbichler P, Volz SE, Joung J, van der Oost J, Regev A, Koonin EV, Zhang F. Cell. 2015 Sep 23. pii: S0092-8674(15)01200-3. doi: 10.1016/j.cell.2015.09.038. 10.1016/j.cell.2015.09.038 PubMed 26422227 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
质粒图谱.png)
载体简介
载体序列
LOCUS pY010 (pcDNA3.1-hAsCpf1) 9464 bp DNA SYN
DEFINITION pY010 (pcDNA3.1-hAsCpf1)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..9464
/organism="pY010 (pcDNA3.1-hAsCpf1)"
/mol_type="other DNA"
promoter 236..852
/label="CMV_immearly_promoter"
misc_feature 315..602
/label="CAG_enhancer"
misc_feature 769..789
/label="CMV_fwd_primer"
promoter 863..881
/label="T7_promoter"
CDS 929..4987
/label="ORF frame 2"
CDS complement(1663..2199)
/label="ORF frame 3"
misc_feature 4904..4930
/label="HA"
misc_feature complement(5058..5075)
/label="BGH_rev_primer"
terminator 5061..5288
/label="bGH_PA_terminator"
rep_origin 5351..5657
/label="f1_origin"
misc_feature complement(5771..5791)
/label="pBABE_3_primer"
misc_feature complement(5777..5992)
/label="SV40_enhancer"
promoter 5789..6057
/label="SV40_promoter"
rep_origin 5956..6033
/label="SV40_origin"
misc_feature 6018..6037
/label="SV40pro_F_primer"
CDS 6172..6966
/label="ORF frame 1"
gene 6175..6963
/label="NeoR/KanR"
/gene="NeoR/KanR"
CDS complement(6481..7017)
/label="ORF frame 3"
terminator 7143..7262
/label="SV40_PA_terminator"
misc_feature 7231..7250
/label="EBV_rev_primer"
promoter complement(7306..7324)
/label="M13_reverse_primer"
misc_feature complement(7323..7345)
/label="M13_pUC_rev_primer"
promoter complement(7359..7388)
/label="lac_promoter"
gene complement(8468..9328)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(8468..9328)
/label="ORF frame 2"
promoter complement(9370..9398)
/label="AmpR_promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttggta ccgccaccAT GACACAGTTC GAGGGCTTTA CCAACCTGTA
961 TCAGGTGAGC AAGACACTGC GGTTTGAGCT GATCCCACAG GGCAAGACCC TGAAGCACAT
1021 CCAGGAGCAG GGCTTCATCG AGGAGGACAA GGCCCGCAAT GATCACTACA AGGAGCTGAA
1081 GCCCATCATC GATCGGATCT ACAAGACCTA TGCCGACCAG TGCCTGCAGC TGGTGCAGCT
1141 GGATTGGGAG AACCTGAGCG CCGCCATCGA CTCCTATAGA AAGGAGAAAA CCGAGGAGAC
1201 AAGGAACGCC CTGATCGAGG AGCAGGCCAC ATATCGCAAT GCCATCCACG ACTACTTCAT
1261 CGGCCGGACA GACAACCTGA CCGATGCCAT CAATAAGAGA CACGCCGAGA TCTACAAGGG
1321 CCTGTTCAAG GCCGAGCTGT TTAATGGCAA GGTGCTGAAG CAGCTGGGCA CCGTGACCAC
1381 AACCGAGCAC GAGAACGCCC TGCTGCGGAG CTTCGACAAG TTTACAACCT ACTTCTCCGG
1441 CTTTTATGAG AACAGGAAGA ACGTGTTCAG CGCCGAGGAT ATCAGCACAG CCATCCCACA
1501 CCGCATCGTG CAGGACAACT TCCCCAAGTT TAAGGAGAAT TGTCACATCT TCACACGCCT
1561 GATCACCGCC GTGCCCAGCC TGCGGGAGCA CTTTGAGAAC GTGAAGAAGG CCATCGGCAT
1621 CTTCGTGAGC ACCTCCATCG AGGAGGTGTT TTCCTTCCCT TTTTATAACC AGCTGCTGAC
1681 ACAGACCCAG ATCGACCTGT ATAACCAGCT GCTGGGAGGA ATCTCTCGGG AGGCAGGCAC
1741 CGAGAAGATC AAGGGCCTGA ACGAGGTGCT GAATCTGGCC ATCCAGAAGA ATGATGAGAC
1801 AGCCCACATC ATCGCCTCCC TGCCACACAG ATTCATCCCC CTGTTTAAGC AGATCCTGTC
1861 CGATAGGAAC ACCCTGTCTT TCATCCTGGA GGAGTTTAAG AGCGACGAGG AAGTGATCCA
1921 GTCCTTCTGC AAGTACAAGA CACTGCTGAG AAACGAGAAC GTGCTGGAGA CAGCCGAGGC
1981 CCTGTTTAAC GAGCTGAACA GCATCGACCT GACACACATC TTCATCAGCC ACAAGAAGCT
2041 GGAGACAATC AGCAGCGCCC TGTGCGACCA CTGGGATACA CTGAGGAATG CCCTGTATGA
2101 GCGGAGAATC TCCGAGCTGA CAGGCAAGAT CACCAAGTCT GCCAAGGAGA AGGTGCAGCG
2161 CAGCCTGAAG CACGAGGATA TCAACCTGCA GGAGATCATC TCTGCCGCAG GCAAGGAGCT
2221 GAGCGAGGCC TTCAAGCAGA AAACCAGCGA GATCCTGTCC CACGCACACG CCGCCCTGGA
2281 TCAGCCACTG CCTACAACCC TGAAGAAGCA GGAGGAGAAG GAGATCCTGA AGTCTCAGCT
2341 GGACAGCCTG CTGGGCCTGT ACCACCTGCT GGACTGGTTT GCCGTGGATG AGTCCAACGA
2401 GGTGGACCCC GAGTTCTCTG CCCGGCTGAC CGGCATCAAG CTGGAGATGG AGCCTTCTCT
2461 GAGCTTCTAC AACAAGGCCA GAAATTATGC CACCAAGAAG CCCTACTCCG TGGAGAAGTT
2521 CAAGCTGAAC TTTCAGATGC CTACACTGGC CTCTGGCTGG GACGTGAATA AGGAGAAGAA
2581 CAATGGCGCC ATCCTGTTTG TGAAGAACGG CCTGTACTAT CTGGGCATCA TGCCAAAGCA
2641 GAAGGGCAGG TATAAGGCCC TGAGCTTCGA GCCCACAGAG AAAACCAGCG AGGGCTTTGA
2701 TAAGATGTAC TATGACTACT TCCCTGATGC CGCCAAGATG ATCCCAAAGT GCAGCACCCA
2761 GCTGAAGGCC GTGACAGCCC ACTTTCAGAC CCACACAACC CCCATCCTGC TGTCCAACAA
2821 TTTCATCGAG CCTCTGGAGA TCACAAAGGA GATCTACGAC CTGAACAATC CTGAGAAGGA
2881 GCCAAAGAAG TTTCAGACAG CCTACGCCAA GAAAACCGGC GACCAGAAGG GCTACAGAGA
2941 GGCCCTGTGC AAGTGGATCG ACTTCACAAG GGATTTTCTG TCCAAGTATA CCAAGACAAC
3001 CTCTATCGAT CTGTCTAGCC TGCGGCCATC CTCTCAGTAT AAGGACCTGG GCGAGTACTA
3061 TGCCGAGCTG AATCCCCTGC TGTACCACAT CAGCTTCCAG AGAATCGCCG AGAAGGAGAT
3121 CATGGATGCC GTGGAGACAG GCAAGCTGTA CCTGTTCCAG ATCTATAACA AGGACTTTGC
3181 CAAGGGCCAC CACGGCAAGC CTAATCTGCA CACACTGTAT TGGACCGGCC TGTTTTCTCC
3241 AGAGAACCTG GCCAAGACAA GCATCAAGCT GAATGGCCAG GCCGAGCTGT TCTACCGCCC
3301 TAAGTCCAGG ATGAAGAGGA TGGCACACCG GCTGGGAGAG AAGATGCTGA ACAAGAAGCT
3361 GAAGGATCAG AAAACCCCAA TCCCCGACAC CCTGTACCAG GAGCTGTACG ACTATGTGAA
3421 TCACAGACTG TCCCACGACC TGTCTGATGA GGCCAGGGCC CTGCTGCCCA ACGTGATCAC
3481 CAAGGAGGTG TCTCACGAGA TCATCAAGGA TAGGCGCTTT ACCAGCGACA AGTTCTTTTT
3541 CCACGTGCCT ATCACACTGA ACTATCAGGC CGCCAATTCC CCATCTAAGT TCAACCAGAG
3601 GGTGAATGCC TACCTGAAGG AGCACCCCGA GACACCTATC ATCGGCATCG ATCGGGGCGA
3661 GAGAAACCTG ATCTATATCA CAGTGATCGA CTCCACCGGC AAGATCCTGG AGCAGCGGAG
3721 CCTGAACACC ATCCAGCAGT TTGATTACCA GAAGAAGCTG GACAACAGGG AGAAGGAGAG
3781 GGTGGCAGCA AGGCAGGCCT GGTCTGTGGT GGGCACAATC AAGGATCTGA AGCAGGGCTA
3841 TCTGAGCCAG GTCATCCACG AGATCGTGGA CCTGATGATC CACTACCAGG CCGTGGTGGT
3901 GCTGGAGAAC CTGAATTTCG GCTTTAAGAG CAAGAGGACC GGCATCGCCG AGAAGGCCGT
3961 GTACCAGCAG TTCGAGAAGA TGCTGATCGA TAAGCTGAAT TGCCTGGTGC TGAAGGACTA
4021 TCCAGCAGAG AAAGTGGGAG GCGTGCTGAA CCCATACCAG CTGACAGACC AGTTCACCTC
4081 CTTTGCCAAG ATGGGCACCC AGTCTGGCTT CCTGTTTTAC GTGCCTGCCC CATATACATC
4141 TAAGATCGAT CCCCTGACCG GCTTCGTGGA CCCCTTCGTG TGGAAAACCA TCAAGAATCA
4201 CGAGAGCCGC AAGCACTTCC TGGAGGGCTT CGACTTTCTG CACTACGACG TGAAAACCGG
4261 CGACTTCATC CTGCACTTTA AGATGAACAG AAATCTGTCC TTCCAGAGGG GCCTGCCCGG
4321 CTTTATGCCT GCATGGGATA TCGTGTTCGA GAAGAACGAG ACACAGTTTG ACGCCAAGGG
4381 CACCCCTTTC ATCGCCGGCA AGAGAATCGT GCCAGTGATC GAGAATCACA GATTCACCGG
4441 CAGATACCGG GACCTGTATC CTGCCAACGA GCTGATCGCC CTGCTGGAGG AGAAGGGCAT
4501 CGTGTTCAGG GATGGCTCCA ACATCCTGCC AAAGCTGCTG GAGAATGACG ATTCTCACGC
4561 CATCGACACC ATGGTGGCCC TGATCCGCAG CGTGCTGCAG ATGCGGAACT CCAATGCCGC
4621 CACAGGCGAG GACTATATCA ACAGCCCCGT GCGCGATCTG AATGGCGTGT GCTTCGACTC
4681 CCGGTTTCAG AACCCAGAGT GGCCCATGGA CGCCGATGCC AATGGCGCCT ACCACATCGC
4741 CCTGAAGGGC CAGCTGCTGC TGAATCACCT GAAGGAGAGC AAGGATCTGA AGCTGCAGAA
4801 CGGCATCTCC AATCAGGACT GGCTGGCCTA CATCCAGGAG CTGCGCAACA AAAGGCCGGC
4861 GGCCACGAAA AAGGCCGGCC AGGCAAAAAA GAAAAAGGGA TCCTACCCAT ACGATGTTCC
4921 AGATTACGCT TATCCCTACG ACGTGCCTGA TTATGCATAC CCATATGATG TCCCCGACTA
4981 TGCCTAAGaa ttctgcagat atccagcaca gtggcggccg ctcgagtcta gagggcccgt
5041 ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc
5101 ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa
5161 tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg
5221 gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg
5281 ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc cccacgcgcc
5341 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
5401 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
5461 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
5521 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
5581 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
5641 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
5701 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
5761 ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
5821 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
5881 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
5941 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
6001 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc tgagctattc
6061 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct cccgggagct
6121 tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg catgattgaa
6181 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
6241 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
6301 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
6361 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
6421 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
6481 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
6541 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
6601 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
6661 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
6721 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
6781 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
6841 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
6901 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
6961 ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac
7021 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg
7081 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca
7141 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
7201 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
7261 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt
7321 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa
7381 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac
7441 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg
7501 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc
7561 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat
7621 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca
7681 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc
7741 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc
7801 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
7861 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta
7921 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
7981 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
8041 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag
8101 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga agaacagtat
8161 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat
8221 ccggcaaaca aaccaccgct ggtagcggtt tttttgtttg caagcagcag attacgcgca
8281 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
8341 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
8401 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
8461 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
8521 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
8581 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
8641 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
8701 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
8761 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg
8821 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
8881 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
8941 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
9001 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
9061 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa
9121 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
9181 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
9241 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
9301 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
9361 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
9421 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc
//
