| 质粒名称: | pMSCV-Zeocin |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 75088 |
| 存储实验室: | David Mu |
|
载体骨架基本信息 |
|
| 载体名称: | pMSCV-puromycin |
| 载体出品公司: | Clontech |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 使用HindIII和ClaI位点用zeocin标记替换嘌呤霉素抗性基因 |
| 载体类型: | 哺乳动物表达，逆转录病毒 |
| 筛选标记: | 博莱霉素 |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMSCV-Zeocin was a gift from David Mu (Addgene plasmid # 75088 ; http://n2t.net/addgene:75088 ; RRID:Addgene_75088) |
| 参考文献部分: | Oncogenic cooperation and coamplification of developmental transcription factor genes in lung cancer. Kendall J, Liu Q, Bakleh A, Krasnitz A, Nguyen KC, Lakshmi B, Gerald WL, Powers S, Mu D. Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16663-8. Epub 2007 Oct 9. 10.1073/pnas.0708286104 PubMed 17925434 |
买家导航
pMSCVZeocin载体基本信息
pMSCVZeocin载体图谱质粒图谱和多克隆位点信息
pMSCVZeocin载体简介
pMSCVZeocin载体序列
LOCUS pMSCV-Zeocin 6017 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION retrovirus-mediated gene transfer into mammalian cells.
ACCESSION .
VERSION .
KEYWORDS pMSCV-Zeocin
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6017)
AUTHORS Kendall J, Liu Q, Bakleh A, Krasnitz A, Nguyen KC, Lakshmi B, Gerald
WL, Powers S, Mu D
TITLE Oncogenic cooperation and coamplification of developmental
transcription factor genes in lung cancer.
JOURNAL Proc Natl Acad Sci U S A. 2007 Oct 16;104(42):16663-8. Epub 2007 Oct
9.
PUBMED 17925434
REFERENCE 2 (bases 1 to 6017)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6017
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 579..920
/label=MESV Psi
/note="packaging signal of murine embryonic stem cell
virus"
CDS 987..1403
/label=gag (truncated)
/note="truncated Moloney murine leukemia virus (MMLV) gag
gene lacking the start codon"
primer_bind 1333..1355
/label=pLXSN 5'
/note="Murine stem cell virus, forward primer. Also called
MSCV"
primer_bind 1371..1387
/label=pBABE 5'
/note="Psi packaging signal, forward primer for pBABE
vectors"
promoter 1438..1937
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(1473..1496)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 1870..1889
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 1958..2332
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
LTR 2379..2893
/label=3' LTR
/note="3' long terminal repeat from murine embryonic stem
cell virus"
primer_bind complement(3062..3078)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3062..3078)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3075..3097)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3086..3102
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3110..3140)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3155..3176
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3293..3310)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3464..4052)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3544..3563)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4223..5083)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4846..4865
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5084..5188)
/gene="bla"
/label=AmpR promoter
primer_bind 5256..5274
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(5312..5334)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 5434..5453
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5647..5669
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 5797..5816
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
LTR 6016..515
/label=5' LTR
/note="5' long terminal repeat from murine embryonic stem
cell virus"
ORIGIN
1 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
61 ggaaaataca taactgagaa tagagaagtt cagatcaagg ttaggaacag agagacagca
121 gaatatgggc caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga
181 acagatggtc cccagatgcg gtcccgccct cagcagtttc tagagaacca tcagatgttt
241 ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc
301 gcttctcgct tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc
361 ctcactcggc gcgccagtcc tccgatagac tgcgtcgccc gggtacccgt attcccaata
421 aagcctcttg ctgtttgcat ccgaatcgtg gactcgctga tccttgggag ggtctcctca
481 gattgattga ctgcccacct cgggggtctt tcatttggag gttccaccga gatttggaga
541 cccctgccca gggaccaccg acccccccgc cgggaggtaa gctggccagc ggtcgtttcg
601 tgtctgtctc tgtctttgtg cgtgtttgtg ccggcatcta atgtttgcgc ctgcgtctgt
661 actagttagc taactagctc tgtatctggc ggacccgtgg tggaactgac gagttctgaa
721 cacccggccg caaccctggg agacgtccca gggactttgg gggccgtttt tgtggcccga
781 cctgaggaag ggagtcgatg tggaatccga ccccgtcagg atatgtggtt ctggtaggag
841 acgagaacct aaaacagttc ccgcctccgt ctgaattttt gctttcggtt tggaaccgaa
901 gccgcgcgtc ttgtctgctg cagcgctgca gcatcgttct gtgttgtctc tgtctgactg
961 tgtttctgta tttgtctgaa aattagggcc agactgttac cactccctta agtttgacct
1021 taggtcactg gaaagatgtc gagcggatcg ctcacaacca gtcggtagat gtcaagaaga
1081 gacgttgggt taccttctgc tctgcagaat ggccaacctt taacgtcgga tggccgcgag
1141 acggcacctt taaccgagac ctcatcaccc aggttaagat caaggtcttt tcacctggcc
1201 cgcatggaca cccagaccag gtcccctaca tcgtgacctg ggaagccttg gcttttgacc
1261 cccctccctg ggtcaagccc tttgtacacc ctaagcctcc gcctcctctt cctccatccg
1321 ccccgtctct cccccttgaa cctcctcgtt cgaccccgcc tcgatcctcc ctttatccag
1381 ccctcactcc ttctctaggc gccggaatta gatctctcga ggttaacgaa ttctaccggg
1441 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
1501 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
1561 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
1621 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
1681 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
1741 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
1801 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
1861 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
1921 catctccggg cctttcgacc tgcagcccaa gcttaccatg gccaagttga ccagtgccgt
1981 tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg accggctcgg
2041 gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg acgtgaccct
2101 gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct gggtgtgggt
2161 gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga acttccggga
2221 cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg agttcgccct
2281 gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact gaatcgataa
2341 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
2401 ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat aactgagaat
2461 agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc aaacaggata
2521 tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc ccagatgcgg
2581 tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc caaggacctg
2641 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
2701 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg cgccagtcct
2761 ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
2821 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
2881 gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg agggtaggag
2941 tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat actccaatac
3001 tcctgaaata gttcattatg gacagcgcag aagagctggg gagaattaat tcgtaatcat
3061 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
3121 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
3181 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
3241 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
3301 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
3361 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
3421 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
3481 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
3541 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
3601 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
3661 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
3721 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
3781 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
3841 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
3901 gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
3961 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
4021 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
4081 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
4141 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
4201 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
4261 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
4321 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
4381 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
4441 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
4501 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
4561 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
4621 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
4681 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
4741 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
4801 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
4861 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
4921 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
4981 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
5041 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
5101 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
5161 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
5221 aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
5281 gtctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg
5341 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
5401 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
5461 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc
5521 gccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
5581 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
5641 ggttttccca gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat
5701 ggcgcccaac agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct
5761 catgagcccg aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc
5821 agcaaccgca cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt
5881 agtccaattt gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata
5941 tcaccagctg aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct
6001 ccagaaaaag gggggaa
//
