³öÆ·¹«Ë¾: | Invitrogen |
---|---|
ÔØÌåÃû³Æ: | pENTR5-UbCp |
ÖÊÁ£ÀàÐÍ: | GatewayÔØÌ壻ÈëÃÅÔØÌå |
¸ß¿½±´/µÍ¿½±´: | ¸ß¿½±´ |
Æô¶¯×Ó: | ÎÞ |
¿Ë¡·½·¨: | ÏÞÖÆÐÔÄÚÇÐø£»¶à¿Ë¡λµã |
ÔØÌå´óС: | 2720 bp |
5' ²âÐòÒýÎï¼°ÐòÁÐ: | pENTR-F: CTACAAACTCTTCCTGTTAGTTAG |
3' ²âÐòÒýÎï¼°ÐòÁÐ: | pENTR-R: ATGGCTCATAACACCCCTTG |
ÔØÌå±êÇ©: | ÎÞ |
ÔØÌ忹ÐÔ: | ¿¨ÄÇùËØ |
ÕæºËɸѡ±ê¼Ç: | ÎÞ |
±¸×¢: |
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LOCUS Exported 3861 bp ds-DNA circular SYN 13-AUG-2016 KEYWORDS pENTR5-UbCp SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3861) AUTHORS Biofeng Lab. TITLE Direct Submission FEATURES Location/Qualifiers source 1..3861 /organism="synthetic DNA construct" /mol_type="other DNA" primer_bind 546..562 /note="M13 fwd" /note="common sequencing primer, one of multiple similar variants" protein_bind 601..697 /gene="mutant version of attL" /bound_moiety="LR Clonase(TM)" /note="attL4" /note="recombination site for the Gateway(R) LR reaction" promoter 698..1906 /note="Ubc promoter" /note="human Ubc promoter" protein_bind 1907..2030 /gene="mutant version of attR" /bound_moiety="LR Clonase(TM)" /note="attR1" /note="recombination site for the Gateway(R) LR reaction" promoter complement(2103..2121) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(2126..2142) /note="M13 rev" /note="common sequencing primer, one of multiple similar variants" CDS 2255..3064 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin in bacteria or G418 (Geneticin(R)) in eukaryotes" /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" rep_origin 3211..3799 /direction=RIGHT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" ORIGIN 1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga 61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aaacggaaga 121 gcgcccaata cgcaaaccgc cctctccccg cgcgttggcc gattcattaa atgcagctgg 181 cacgacaggt tttcccgact ggaaagcggg cagtggagcg caacgcaatt aatacccgta 241 cccgcttacc caggaaaagt ttgtaaaaac gcaaaaaagg ccatccctcc aggatggcct 301 tctgcttaat ttggatgcct ggcaagttta tggggggggt ctgcccgcca ccctccgggc 361 cgttgcttcc acaacgttca aatccgctcc ccgcggattt gtcctactca ggagagcgtt 421 caccgacaaa caacagataa aacgaaaggc ccagttcccg actgagcctt tcgttttatt 481 tgatgcctgg cagttcccta ctctcgcgtt aacgctagca tggatgtttt cccagtcacg 541 acgttgtaaa acgacggcca gtcttaagct cgggcccgcg ttaacgctac catggagctc 601 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgataagcaa 661 tgctttttta taatgccaac tttgtataga aaagttgggc ctcgcgccgg gttttggcgc 721 ctcccgcggg cgcccccctc ctcacggcga gcgctgccac gtcagacgaa gggcgcagga 781 gcgtcctgat ccttccgccc ggacgctcag gacagcggcc cgctgctcat aagactcggc 841 cttagaaccc cagtatcagc agaaggacat tttaggacgg gacttgggtg actctagggc 901 actggttttc tttccagaga gcggaacagg cgaggaaaag tagtcccttc tcggcgattc 961 tgcggaggga tctccgtggg gcggtgaacg ccgatgatta tataaggacg cgccgggtgt 1021 ggcacagcta gttccgtcgc agccgggatt tgggtcgcgg ttcttgtttg tggatcgctg 1081 tgatcgtcac ttggtgagta gcgggctgct gggctggccg gggctttcgt ggccgccggg 1141 ccgctcggtg ggacggaagc gtgtggagag accgccaagg gctgtagtct gggtccgcga 1201 gcaaggttgc cctgaactgg gggttggggg gagcgcagca aaatggcggc tgttcccgag 1261 tcttgaatgg aagacgcttg tgaggcgggc tgtgaggtcg ttgaaacaag gtggggggca 1321 tggtgggcgg caagaaccca aggtcttgag gccttcgcta atgcgggaaa gctcttattc 1381 gggtgagatg ggctggggca ccatctgggg accctgacgt gaagtttgtc actgactgga 1441 gaactcggtt tgtcgtctgt tgcgggggcg gcagttatgc ggtgccgttg ggcagtgcac 1501 ccgtaccttt gggagcgcgc gccctcgtcg tgtcgtgacg tcacccgttc tgttggctta 1561 taatgcaggg tggggccacc tgccggtagg tgtgcggtag gcttttctcc gtcgcaggac 1621 gcagggttcg ggcctagggt aggctctcct gaatcgacag gcgccggacc tctggtgagg 1681 ggagggataa gtgaggcgtc agtttctttg gtcggtttta tgtacctatc ttcttaagta 1741 gctgaagctc cggttttgaa ctatgcgctc ggggttggcg agtgtgtttt gtgaagtttt 1801 ttaggcacct tttgaaatgt aatcatttgg gtcaatatgt aattttcagt gttagactag 1861 taaattgtcc gctaaattct ggccgttttt ggcttttttg ttagaccaag tttgtacaaa 1921 aaagttgaac gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat 1981 aaaaaacaga ctacataata ctgtaaaaca caacatatgc agtcactatg aatcaactac 2041 ttagatggta ttagtgacct gtagaattcg agctctagag ctgcagggcg gccgcgatat 2101 cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc 2161 tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact 2221 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 2281 gaggccgcga ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga 2341 taatgtcggg caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga 2401 gttgtttctg aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag 2461 actaaactgg ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc 2521 tgatgatgca tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga 2581 agaatatcct gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt 2641 gcattcgatt cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca 2701 ggcgcaatca cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa 2761 tggctggcct gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga 2821 ttcagtcgtc actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt 2881 aataggttgt attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat 2941 cctatggaac tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata 3001 tggtattgat aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt 3061 ctaatcagaa ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga 3121 cggcgcaagc tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc 3181 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtactctg 3241 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct 3301 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct 3361 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct 3421 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg 3481 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc 3541 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga 3601 gcattgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg 3661 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta 3721 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg 3781 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg 3841 ctggcctttt gctcacatgt t //
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