| 质粒名称: | T7-TPase |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 51818 |
| 存储实验室: | Elly Tanaka |
|
载体骨架基本信息 |
|
| 载体名称: | pBluscript II SK+ |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | -- |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Tol2 Transposase |
| 物种来源: | O. latipes |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | T7 |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XhoI(未破坏) |
| 3' 克隆位点: | XbaI(未破坏) |
| 5' 测序引物 | M13 Universal |
| 3' 测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | T7-TPase was a gift from Elly Tanaka (Addgene plasmid # 51818 ; http://n2t.net/addgene:51818 ; RRID:Addgene_51818) |
| 参考文献部分: | Optimized axolotl (Ambystoma mexicanum) husbandry, breeding, metamorphosis, transgenesis and tamoxifen-mediated recombination. Khattak S, Murawala P, Andreas H, Kappert V, Schuez M, Sandoval-Guzman T, Crawford K, Tanaka EM. Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub 2014 Feb 6. 10.1038/nprot.2014.040 PubMed 24504478 |
买家导航
T7TPase载体基本信息
T7TPase载体图谱质粒图谱和多克隆位点信息
T7TPase载体简介
T7TPase载体序列
LOCUS T7-TPase 5338 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION For in vitro transcription of Tol2 transposase using T7 Promoter.
ACCESSION .
VERSION .
KEYWORDS T7-TPase
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5338)
AUTHORS Khattak S, Murawala P, Andreas H, Kappert V, Schuez M,
Sandoval-Guzman T, Crawford K, Tanaka EM
TITLE Optimized axolotl (Ambystoma mexicanum) husbandry, breeding,
metamorphosis, transgenesis and tamoxifen-mediated recombination.
JOURNAL Nat Protoc. 2014 Mar;9(3):529-40. doi: 10.1038/nprot.2014.040. Epub
2014 Feb 6.
PUBMED 24504478
REFERENCE 2 (bases 1 to 5338)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5338
/organism="synthetic DNA construct"
/mol_type="other DNA"
rep_origin complement(3..458)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(140..161)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 352..371
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 585..607
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 599..616
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 600..616
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 649..668
/label=T7
/note="T7 promoter, forward primer"
promoter 649..667
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(3013..3031)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 3027..3074
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(3127..3147)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(3127..3145)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(3189..3205)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3189..3205)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3202..3224)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3213..3229
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3237..3267)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 3282..3303
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(3420..3437)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(3591..4179)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3671..3690)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4350..5210)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4973..4992
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(5211..5315)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
601 taaaacgacg gccagtgagc gcgtagggat aacagggtaa tgcgcgcgta atacgactca
661 ctatagggcg aattgggtac cgggcccccc ctcgaggtcg agtcacatct attaccacaa
721 tgcacagcac cttgacctgg aaattaggga aattataaca gtcaatcagt ggaagaaaat
781 ggaggaagta tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga
841 tcaagagcac ccgtggccgt atcttcgcga attcttttct ttaagtggtg taaataaaga
901 ttcattcaag atgaaatgtg tcctctgtct cccgcttaat aaagaaatat cggccttcaa
961 aagttcgcca tcaaacctaa ggaagcatat tgagagaatg cacccaaatt acctcaaaaa
1021 ctactctaaa ttgacagcac agaagagaaa gatcgggacc tccacccatg cttccagcag
1081 taagcaactg aaagttgact cagttttccc agtcaaacat gtgtctccag tcactgtgaa
1141 caaagctata ttaaggtaca tcattcaagg acttcatcct ttcagcactg ttgatctgcc
1201 atcatttaaa gagctgatta gtacactgca gcctggcatt tctgtcatta caaggcctac
1261 tttacgctcc aagatagctg aagctgctct gatcatgaaa cagaaagtga ctgctgccat
1321 gagtgaagtt gaatggattg caaccacaac ggattgttgg actgcacgta gaaagtcatt
1381 cattggtgta actgctcact ggatcaaccc tggaagtctt gaaagacatt ccgctgcact
1441 tgcctgcaaa agattaatgg gctctcatac ttttgaggta ctggccagtg ccatgaatga
1501 tatccactca gagtatgaaa tacgtgacaa ggttgtttgc acaaccacag acagtggttc
1561 caactttatg aaggctttca gagtttttgg tgtggaaaac aatgatatcg agactgaggc
1621 aagaaggtgt gaaagtgatg acactgattc tgaaggctgt ggtgagggaa gtgatggtgt
1681 ggaattccaa gatgcctcac gagtcctgga ccaagacgat ggcttcgaat tccagctacc
1741 aaaacatcaa aagtgtgcct gtcacttact taacctagtc tcaagcgttg atgcccaaaa
1801 agctctctca aatgaacact acaagaaact ctacagatct gtctttggca aatgccaagc
1861 tttatggaat aaaagcagcc gatcggctct agcagctgaa gctgttgaat cagaaagccg
1921 gcttcagctt ttaaggccaa accaaacgcg gtggaattca acttttatgg ctgttgacag
1981 aattcttcaa atttgcaaag aagcaggaga aggcgcactt cggaatatat gcacctctct
2041 tgaggttcca atgtttaatc cagcagaaat gctgttcttg acagagtggg ccaacacaat
2101 gcgtccagtt gcaaaagtac tcgacatctt gcaagcggaa acgaatacac agctggggtg
2161 gctgctgcct agtgtccatc agttaagctt gaaacttcag cgactccacc attctctcag
2221 gtactgtgac ccacttgtgg atgccctaca acaaggaatc caaacacgat tcaagcatat
2281 gtttgaagat cctgagatca tagcagctgc catccttctc cctaaatttc ggacctcttg
2341 gacaaatgat gaaaccatca taaaacgagg catggactac atcagagtgc atctggagcc
2401 tttggaccac aagaaggaat tggccaacag ttcatctgat gatgaagatt ttttcgcttc
2461 tttgaaaccg acaacacatg aagccagcaa agagttggat ggatatctgg cctgtgtttc
2521 agacaccagg gagtctctgc tcacgtttcc tgctatttgc agcctctcta tcaagactaa
2581 tacacctctt cccgcatcgg ctgcctgtga gaggcttttc agcactgcag gattgctttt
2641 cagccccaaa agagctaggc ttgacactaa caattttgag aatcagcttc tactgaagtt
2701 aaatctgagg ttttacaact ttgagtagcg tgtactggca ttagattgtc tgtcttatag
2761 tttgataatt aaatacaaac agttctaaag caggataaaa ccttgtatgc atttcattta
2821 atgttttttg agattaaaag cttaaacaag aaaaaaaaaa aaaaaaaaaa aaaaaagtac
2881 tagtcgagcc tctagagcgg ccgccaccgc ggcgagacac ccggatcgcg ccaaaaaaaa
2941 aaaaaaaaaa aaaaaaaaaa ccggatccgg ctgctaacaa agcccgaaag gaagctgagt
3001 tggctgctgc caccgctgag caataactag cataacccct tggggcctct aaacgggtct
3061 tgaggggttt tttgctgaaa ggaggaacta tatccggata tcccgcaaga gctccagctt
3121 ttgttccctt tagtgagggt taattgcgcg cattaccctg ttatccctac gcgcttggcg
3181 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
3241 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
3301 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
3361 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
3421 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
3481 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
3541 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
3601 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
3661 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
3721 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
3781 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
3841 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
3901 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
3961 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
4021 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
4081 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
4141 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
4201 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
4261 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
4321 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
4381 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
4441 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
4501 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
4561 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
4621 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
4681 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
4741 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
4801 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
4861 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
4921 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
4981 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
5041 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
5101 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
5161 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
5221 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
5281 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccac
//
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