| 质粒名称: | 3xmScarletI_UtrCH |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 112962 |
| 存储实验室: | Dorus Gadella |
|
载体骨架基本信息 |
|
| 载体名称: | pEGFP-C1 |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | UtrCH |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | 3x mScarlet-I（插入件上的N端子） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | 3xmScarletI_UtrCH was a gift from Dorus Gadella (Addgene plasmid # 112962 ; http://n2t.net/addgene:112962 ; RRID:Addgene_112962) |
| 参考文献部分: | Robust and Bright Genetically Encoded Fluorescent Markers for Highlighting Structures and Compartments in Mammalian Cells. Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S, Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J. bioRxiv 160374 10.1101/160374 |
买家导航
3xmScarletIUtrCH载体基本信息
3xmScarletIUtrCH载体图谱质粒图谱和多克隆位点信息
3xmScarletIUtrCH载体简介
3xmScarletIUtrCH载体序列
LOCUS 3xmScarletI_UtrCH 6914 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION In vivo visualization of F-actin (can be used for colocalization
studies).
ACCESSION .
VERSION .
KEYWORDS 3xmScarletI_UtrCH
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6914)
AUTHORS Chertkova AO, Mastop M, Postma M, van Bommel N, van der Niet S,
Batenburg KL, Joosen L, Gadella TWJ, Okada Y, Goedhart J
TITLE Robust and Bright Genetically Encoded Fluorescent Markers for
Highlighting Structures and Compartments in Mammalian Cells
JOURNAL bioRxiv 160374
REFERENCE 2 (bases 1 to 6914)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6914
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 459..479
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
regulatory 547..556
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 553..1245
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELY"
CDS 1264..1956
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELY"
CDS 1975..2670
/codon_start=1
/product="bright monomeric red fluorescent protein evolved
from a synthetic template (Bindels et al., 2016)"
/label=mScarlet-I
/note="matures faster than mScarlet"
/translation="MVSKGEAVIKEFMRFKVHMEGSMNGHEFEIEGEGEGRPYEGTQTA
KLKVTKGGPLPFSWDILSPQFMYGSRAFIKHPADIPDYYKQSFPEGFKWERVMNFEDGG
AVTVTQDTSLEDGTLIYKVKLRGTNFPPDGPVMQKKTMGWEASTERLYPEDGVLKGDIK
MALRLKDGGRYLADFKTTYKAKKPVQMPGAYNVDRKLDITSHNEDYTVVEQYERSEGRH
STGGMDELYK"
misc_feature 3466..3518
/label=MCS
/note="multiple cloning site"
polyA_signal 3642..3763
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(3679..3698)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 3733..3752
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(3770..4225)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3907..3928)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 4119..4138
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 4252..4356
/gene="bla"
/label=AmpR promoter
promoter 4358..4715
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(4381..4401)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 4566..4701
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 4628..4647
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 4750..5544
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4804..4823)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5414..5433
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(5732..5751)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 5776..5823
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 6152..6740
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6641..6660
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
541 ccggtcgcca ccatggtgag caagggcgag gcagtgatca aggagttcat gcggttcaag
601 gtgcacatgg agggctccat gaacggccac gagttcgaga tcgagggcga gggcgagggc
661 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
721 ttctcctggg acatcctgtc ccctcagttc atgtacggct ccagggcctt catcaagcac
781 cccgccgaca tccccgacta ctataagcag tccttccccg agggcttcaa gtgggagcgc
841 gtgatgaact tcgaggacgg cggcgccgtg accgtgaccc aggacacctc cctggaggac
901 ggcaccctga tctacaaggt gaagctccgc ggcaccaact tccctcctga cggccccgta
961 atgcagaaga agacaatggg ctgggaagcg tccaccgagc ggttgtaccc cgaggacggc
1021 gtgctgaagg gcgacattaa gatggccctg cgcctgaagg acggcggccg ctacctggcg
1081 gacttcaaga ccacctacaa ggccaagaag cccgtgcaga tgcccggcgc ctacaacgtc
1141 gaccgcaagt tggacatcac ctcccacaac gaggactaca ccgtggtgga acagtacgaa
1201 cgctccgagg gccgccactc caccggcggc atggacgagc tgtaccaggg tggcagcggt
1261 ggcatggtga gcaagggcga ggcagtgatc aaggagttca tgcggttcaa ggtgcacatg
1321 gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg ccgcccctac
1381 gagggcaccc agaccgccaa gctgaaggtg accaagggtg gccccctgcc cttctcctgg
1441 gacatcctgt cccctcagtt catgtacggc tccagggcct tcatcaagca ccccgccgac
1501 atccccgact actataagca gtccttcccc gagggcttca agtgggagcg cgtgatgaac
1561 ttcgaggacg gcggcgccgt gaccgtgacc caggacacct ccctggagga cggcaccctg
1621 atctacaagg tgaagctccg cggcaccaac ttccctcctg acggccccgt aatgcagaag
1681 aagacaatgg gctgggaagc gtccaccgag cggttgtacc ccgaggacgg cgtgctgaag
1741 ggcgacatta agatggccct gcgcctgaag gacggcggcc gctacctggc ggacttcaag
1801 accacctaca aggccaagaa gcccgtgcag atgcccggcg cctacaacgt cgaccgcaag
1861 ttggacatca cctcccacaa cgaggactac accgtggtgg aacagtacga acgctccgag
1921 ggccgccact ccaccggcgg catggacgag ctgtaccagg gtggcagcgg tggcatggtg
1981 agcaagggcg aggcagtgat caaggagttc atgcggttca aggtgcacat ggagggctcc
2041 atgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
2101 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttctcctg ggacatcctg
2161 tcccctcagt tcatgtacgg ctccagggcc ttcatcaagc accccgccga catccccgac
2221 tactataagc agtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
2281 ggcggcgccg tgaccgtgac ccaggacacc tccctggagg acggcaccct gatctacaag
2341 gtgaagctcc gcggcaccaa cttccctcct gacggccccg taatgcagaa gaagacaatg
2401 ggctgggaag cgtccaccga gcggttgtac cccgaggacg gcgtgctgaa gggcgacatt
2461 aagatggccc tgcgcctgaa ggacggcggc cgctacctgg cggacttcaa gaccacctac
2521 aaggccaaga agcccgtgca gatgcccggc gcctacaacg tcgaccgcaa gttggacatc
2581 acctcccaca acgaggacta caccgtggtg gaacagtacg aacgctccga gggccgccac
2641 tccaccggcg gcatggacga gctgtacaag tccggaacca tggccaagta tggagaacat
2701 gaagccagtc ctgacaatgg gcagaacgaa ttcagtgata tcattaagtc cagatctgat
2761 gaacacaatg acgtacagaa gaaaaccttt accaaatgga taaatgctcg attttcaaag
2821 agtgggaaac cacccatcaa tgatatgttc acagacctca aagatggaag gaagctattg
2881 gatcttctag aaggcctcac aggaacatca ctgccaaagg aacgtggttc cacaagggta
2941 catgccttaa ataacgtcaa cagagtgctg caggttttac atcagaacaa tgtggaatta
3001 gtgaatatag ggggaaccga cattgtggat ggaaatcaca aactgacttt ggggttactt
3061 tggagcatca ttttgcactg gcaggtgaaa gatgtcatga aggatgtcat gtcggacctg
3121 cagcagacga acagtgagaa gatcctgctc agctgggtgc gtcagaccac caggccctac
3181 agccaagtca acgtcctcaa cttcaccacc agctggacag atggactcgc ctttaatgct
3241 gtcctccacc gacataaacc tgatctcttc agctgggata aagttgtcaa aatgtcacca
3301 attgagagac ttgaacatgc cttcagcaag gctcaaactt atttgggaat tgaaaagctg
3361 ttagatcctg aagatgttgc cgttcggctt cctgacaaga aatccataat tatgtattta
3421 acatctttgt ttgaggtgct acctcagcaa gtcaccatag actaactcga gctcaagctt
3481 cgaattctgc agtcgacggt accgcgggcc cgggatccac cggatctaga taactgatca
3541 taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc ccacacctcc
3601 ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt attgcagctt
3661 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
3721 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttaaggcgta aattgtaagc
3781 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
3841 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
3901 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
3961 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
4021 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
4081 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
4141 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
4201 cttaatgcgc cgctacaggg cgcgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
4261 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
4321 tgataaatgc ttcaataata ttgaaaaagg aagagtcctg aggcggaaag aaccagctgt
4381 ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc
4441 aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag
4501 gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc
4561 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
4621 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
4681 gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac aggatgagga
4741 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
4801 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
4861 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
4921 aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
4981 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
5041 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
5101 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
5161 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
5221 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgagc
5281 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
5341 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
5401 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
5461 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
5521 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
5581 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
5641 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
5701 agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca ataccggaag
5761 gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt gggtcgtttg
5821 ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca ccgagacccc
5881 attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca agttcgggtg
5941 aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc tcaggttact
6001 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
6061 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
6121 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
6181 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
6241 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
6301 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6361 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6421 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
6481 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
6541 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
6601 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6661 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6721 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
6781 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
6841 ttaccgccat gcattagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
6901 tatatggagt tccg
//
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