| 出品公司: |
Invitrogen
|
|---|---|
| 载体名称: | pUB6/V5 His A |
| 质粒类型: | 哺乳细胞表达系统载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | UbC |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5459bp |
| 5' 测序引物及序列: | T7 Fwd：5'd[TAATACGACTCACTATAGGG]3' |
| 3' 测序引物及序列: | -- |
| 载体标签: | 6X His, V5 |
| 载体抗性: | Ampicillin |
| 筛选标记: | Blasticidin |
| 备注: | 可以严格控制表达水平 |
| 产品目录号: | V25020 |
| 稳定性: | 瞬表达 |
| 组成型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pUB6/V5 His C载体 pUB6-V5HisC质粒基本信息
pUB6/V5 His C质粒图谱 pUB6-V5HisC载体图谱和pUB6/V5 His C载体序列 pUB6-V5HisC质粒序列多克隆位点信息
pUB6/V5 His C质粒 pUB6-V5HisC载体简介
pUB6/V5 His C质粒序列 pUB6-V5HisC载体序列
LOCUS pUB6/V5/His C 5459 bp DNA SYN
DEFINITION pUB6/V5/His C
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..5459
/organism="pUB6/V5/His C"
/mol_type="other DNA"
promoter 13..1227
/label="hUbC_promoter"
misc_feature complement(1414..1431)
/label="BGH_rev_primer"
terminator 1417..1644
/label="bGH_PA_terminator"
rep_origin 1707..2013
/label="f1_origin"
misc_feature complement(2127..2147)
/label="pBABE_3_primer"
misc_feature complement(2133..2349)
/label="SV40_enhancer"
promoter 2145..2414
/label="SV40_promoter"
rep_origin 2313..2390
/label="SV40_origin"
misc_feature 2375..2394
/label="SV40pro_F_primer"
promoter 2508..2575
/label="EM7_promoter"
gene 2576..2974
/label="blast"
/gene="blast"
terminator 3135..3254
/label="SV40_PA_terminator"
misc_feature 3223..3242
/label="EBV_rev_primer"
promoter complement(3298..3316)
/label="M13_reverse_primer"
misc_feature complement(3315..3337)
/label="M13_pUC_rev_primer"
promoter complement(3351..3380)
/label="lac_promoter"
rep_origin complement(3689..4308)
/label="pBR322_origin"
gene complement(4463..5323)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(4463..5323)
/label="ORF frame 2"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
promoter complement(5365..5393)
/label="AmpR_promoter"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
ORIGIN
1 GACGGATCGG GAGATCTGGC CTCCGCGCCG GGTTTTGGCG CCTCCCGCGG GCGCCCCCCT
61 CCTCACGGCG AGCGCTGCCA CGTCAGACGA AGGGCGCAGG AGCGTCCTGA TCCTTCCGCC
121 CGGACGCTCA GGACAGCGGC CCGCTGCTCA TAAGACTCGG CCTTAGAACC CCAGTATCAG
181 CAGAAGGACA TTTTAGGACG GGACTTGGGT GACTCTAGGG CACTGGTTTT CTTTCCAGAG
241 AGCGGAACAG GCGAGGAAAA GTAGTCCCTT CTCGGCGATT CTGCGGAGGG ATCTCCGTGG
301 GGCGGTGAAC GCCGATGATT ATATAAGGAC GCGCCGGGTG TGGCACAGCT AGTTCCGTCG
361 CAGCCGGGAT TTGGGTCGCG GTTCTTGTTT GTGGATCGCT GTGATCGTCA CTTGGTGAGT
421 AGCGGGCTGC TGGGCTGGCC GGGGCTTTCG TGGCCGCCGG GCCGCTCGGT GGGACGGAAG
481 CGTGTGGAGA GACCGCCAAG GGCTGTAGTC TGGGTCCGCG AGCAAGGTTG CCCTGAACTG
541 GGGGTTGGGG GGAGCGCAGC AAAATGGCGG CTGTTCCCGA GTCTTGAATG GAAGACGCTT
601 GTGAGGCGGG CTGTGAGGTC GTTGAAACAA GGTGGGGGGC ATGGTGGGCG GCAAGAACCC
661 AAGGTCTTGA GGCCTTCGCT AATGCGGGAA AGCTCTTATT CGGGTGAGAT GGGCTGGGGC
721 ACCATCTGGG GACCCTGACG TGAAGTTTGT CACTGACTGG AGAACTCGGT TTGTCGTCTG
781 TTGCGGGGGC GGCAGTTATG CGGTGCCGTT GGGCAGTGCA CCCGTACCTT TGGGAGCGCG
841 CGCCCTCGTC GTGTCGTGAC GTCACCCGTT CTGTTGGCTT ATAATGCAGG GTGGGGCCAC
901 CTGCCGGTAG GTGTGCGGTA GGCTTTTCTC CGTCGCAGGA CGCAGGGTTC GGGCCTAGGG
961 TAGGCTCTCC TGAATCGACA GGCGCCGGAC CTCTGGTGAG GGGAGGGATA AGTGAGGCGT
1021 CAGTTTCTTT GGTCGGTTTT ATGTACCTAT CTTCTTAAGT AGCTGAAGCT CCGGTTTTGA
1081 ACTATGCGCT CGGGGTTGGC GAGTGTGTTT TGTGAAGTTT TTTAGGCACC TTTTGAAATG
1141 TAATCATTTG GGTCAATATG TAATTTTCAG TGTTAGACTA GTAAATTGTC CGCTAAATTC
1201 TGGCCGTTTT TGGCTTTTTT GTTAGACGAA GCTTGGTACC GAGCTCGGAT CCACTAGTCC
1261 AGTGTGGTGG AATTCTGCAG ATATCCAGCA CAGTGGCGGC CGCTCGAGGT CACCCATTCG
1321 AAGGTAAGCC TATCCCTAAC CCTCTCCTCG GTCTCGATTC TACGCGTACC GGTCATCATC
1381 ACCATCACCA TTGAGTTTAA ACCCGCTGAT CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC
1441 CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC ACTCCCACTG
1501 TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GAGTAGGTGT CATTCTATTC
1561 TGGGGGGTGG GGTGGGGCAG GACAGCAAGG GGGAGGATTG GGAAGACAAT AGCAGGCATG
1621 CTGGGGATGC GGTGGGCTCT ATGGCTTCTG AGGCGGAAAG AACCAGCTGG GGCTCTAGGG
1681 GGTATCCCCA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA
1741 GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT
1801 TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGCATC CCTTTAGGGT
1861 TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC
1921 GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT
1981 TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT
2041 TTGATTTATA AGGGATTTTG GGGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC
2101 AAAAATTTAA CGCGAATTAA TTCTGTGGAA TGTGTGTCAG TTAGGGTGTG GAAAGTCCCC
2161 AGGCTCCCCA GGCAGGCAGA AGTATGCAAA GCATGCATCT CAATTAGTCA GCAACCAGGT
2221 GTGGAAAGTC CCCAGGCTCC CCAGCAGGCA GAAGTATGCA AAGCATGCAT CTCAATTAGT
2281 CAGCAACCAT AGTCCCGCCC CTAACTCCGC CCATCCCGCC CCTAACTCCG CCCAGTTCCG
2341 CCCATTCTCC GCCCCATGGC TGACTAATTT TTTTTATTTA TGCAGAGGCC GAGGCCGCCT
2401 CTGCCTCTGA GCTATTCCAG AAGTAGTGAG GAGGCTTTTT TGGAGGCCTA GGCTTTTGCA
2461 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAGCACGTG TTGACAATTA
2521 ATCATCGGCA TAGTATATCG GCATAGTATA ATACGACAAG GTGAGGAACT AAACCATGGC
2581 CAAGCCTTTG TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG
2641 CATCCCCATC TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT
2701 CTTCACTGGT GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT
2761 GGGCACTGCT GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA
2821 GAACAGGGGC ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC
2881 TGGGATCAAA GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA
2941 ATTGCTGCCC TCTGGTTATG TGTGGGAGGG CTAAGCACTT CGTGGCCGAG GAGCAGGACT
3001 GACACGTGCT ACGAGATTTC GATTCCACCG CCGCCTTCTA TGAAAGGTTG GGCTTCGGAA
3061 TCGTTTTCCG GGACGCCGGC TGGATGATCC TCCAGCGCGG GGATCTCATG CTGGAGTTCT
3121 TCGCCCACCC CAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
3181 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
3241 TCAATGTATC TTATCATGTC TGTATACCGT CGACCTCTAG CTAGAGCTTG GCGTAATCAT
3301 GGTCATAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC AATTCCACAC AACATACGAG
3361 CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT GAGCTAACTC ACATTAATTG
3421 CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC GTGCCAGCTG CATTAATGAA
3481 TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG CTCTTCCGCT TCCTCGCTCA
3541 CTGACTCGCT GCGCTCGGTC GTTCGGCTGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG
3601 TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC
3661 AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC
3721 CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC
3781 TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC
3841 TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCAAT
3901 GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC
3961 ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA
4021 ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG
4081 CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA
4141 GAAGGACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG
4201 GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC
4261 AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT
4321 CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA
4381 GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT
4441 ATGAGTAAAC TTGGTCTGAC AGTTACCAAT GCTTAATCAG TGAGGCACCT ATCTCAGCGA
4501 TCTGTCTATT TCGTTCATCC ATAGTTGCCT GACTCCCCGT CGTGTAGATA ACTACGATAC
4561 GGGAGGGCTT ACCATCTGGC CCCAGTGCTG CAATGATACC GCGAGACCCA CGCTCACCGG
4621 CTCCAGATTT ATCAGCAATA AACCAGCCAG CCGGAAGGGC CGAGCGCAGA AGTGGTCCTG
4681 CAACTTTATC CGCCTCCATC CAGTCTATTA ATTGTTGCCG GGAAGCTAGA GTAAGTAGTT
4741 CGCCAGTTAA TAGTTTGCGC AACGTTGTTG CCATTGCTAC AGGCATCGTG GTGTCACGCT
4801 CGTCGTTTGG TATGGCTTCA TTCAGCTCCG GTTCCCAACG ATCAAGGCGA GTTACATGAT
4861 CCCCCATGTT GTGCAAAAAA GCGGTTAGCT CCTTCGGTCC TCCGATCGTT GTCAGAAGTA
4921 AGTTGGCCGC AGTGTTATCA CTCATGGTTA TGGCAGCACT GCATAATTCT CTTACTGTCA
4981 TGCCATCCGT AAGATGCTTT TCTGTGACTG GTGAGTACTC AACCAAGTCA TTCTGAGAAT
5041 AGTGTATGCG GCGACCGAGT TGCTCTTGCC CGGCGTCAAT ACGGGATAAT ACCGCGCCAC
5101 ATAGCAGAAC TTTAAAAGTG CTCATCATTG GAAAACGTTC TTCGGGGCGA AAACTCTCAA
5161 GGATCTTACC GCTGTTGAGA TCCAGTTCGA TGTAACCCAC TCGTGCACCC AACTGATCTT
5221 CAGCATCTTT TACTTTCACC AGCGTTTCTG GGTGAGCAAA AACAGGAAGG CAAAATGCCG
5281 CAAAAAAGGG AATAAGGGCG ACACGGAAAT GTTGAATACT CATACTCTTC CTTTTTCAAT
5341 ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT
5401 AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGTC
//
pUB6/V5 His C载体图谱 pUB6-V5HisC质粒图谱pdf版和pUB6/V5 His C载体序列 pUB6-V5HisC质粒序列等相关资料下载
pUB6/V5 His C质粒 pUB6-V5HisC载体应用举例
- 上一篇：pUB6/V5 His A
- 下一篇：pCMV-Tag 2B
