| 质粒名称: | pCas9 addgene 167547 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 167547 |
| 存储实验室: | Howard Hang |
|
167547 载体骨架基本信息 |
|
| 载体名称: | pVPL3004 |
| 载体出品公司: | Jan-Peter van Pijkeren |
| 空载体大小: | 9747 bp |
| 完整质粒大小: | 10249 bp |
| 载体修饰: | 红霉素耐药性通过氯霉素乙酰转移酶被氯霉素取代。插入pUC19起源或复制。 |
| 载体类型: | 细菌表达，CRISPR |
| 筛选标记: | -- |
| 载体抗性: | 氯霉素，12.5μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称1: | 氯霉素乙酰转移酶 |
| 插入大小1: | 651 bp |
| 克隆方法1: | Gibson Cloning |
| 5’测序引物1 | TTGAAGTTAAATTAGATGCT |
| 插入基因名称2: | pUC19复制起源 |
| 插入大小2: | 589 bp |
| 克隆方法2: | Gibson Cloning |
| 5’测序引物2 | GAGTCGCTTTTGTAAATTTG |
|
文献引用方法 |
|
| 材料和方法部分: | pCas9 was a gift from Howard Hang (Addgene plasmid # 167547 ; http://n2t.net/addgene:167547 ; RRID:Addgene_167547) |
| 参考文献部分: | RecT recombinase expression enables efficient gene editing in Enterococcus. Chen V, Griffin ME, Maguin P, Varble A, Hang HC. Appl Environ Microbiol. 2021 Jul 7:AEM0084421. doi: 10.1128/AEM.00844-21. 10.1128/AEM.00844-21 PubMed 34232061 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCas9 10247 bp ds-DNA circular SYN 13-MAY-2021
DEFINITION Used for CRISPR-Cas9 mediated recomineering in Enterococcus. Can
clone desired gRNA using BsaI digestion..
ACCESSION .
VERSION .
KEYWORDS pCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10247)
TITLE RecT recombinase expression enables efficient gene editing in
Enterococcus
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 10247)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 13, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10247
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_RNA complement(170..248)
/label=tracrRNA
/note="trans-activating CRISPR RNA for the Streptococcus
pyogenes CRISPR/Cas9 system"
CDS 544..4650
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
misc_feature 4671..4802
/label=crRNA leader
/note="crRNA leader sequence for the Streptococcus pyogenes
CRISPR/Cas system"
repeat_region 4803..4838
/label=DR
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
repeat_region 4869..4904
/label=DR
/note="direct repeat for the Streptococcus pyogenes
CRISPR/Cas system"
primer_bind complement(5086..5102)
/label=SK primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5086..5102)
/label=pBluescriptSK
/note="For pBluescript vector"
rep_origin 6392..6980
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6881..6900
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ttacgaaatc atcctgtgga gcttagtagg tttagcaaga tggcagcgcc taaatgtaga
61 atgataaaag gattaagaga ttaatttccc taaaaatgat aaaacaagcg ttttgaaagc
121 gcttgttttt ttggtttgca gtcagagtag aatagaagta tcaaaaaaag caccgactcg
181 gtgccacttt ttcaagttga taacggacta gccttatttt aacttgctat gctgttttga
241 atggttccaa caagattatt ttataacttt tataacaaat aatcaaggag aaattcaaag
301 aaatttatca gccataaaac aatacttaat actatagaat gataacaaaa taaactactt
361 tttaaaagaa ttttgtgtta taatctattt attattaagt attgggtaat attttttgaa
421 gagatatttt gaaaaagaaa aattaaagca tattaaacta atttcggagg tcattaaaac
481 tattattgaa atcatcaaac tcattatgga tttaatttaa actttttatt ttaggaggca
541 aaaatggata agaaatactc aataggctta gatatcggca caaatagcgt cggatgggcg
601 gtgatcactg atgaatataa ggttccgtct aaaaagttca aggttctggg aaatacagac
661 cgccacagta tcaaaaaaaa tcttataggg gctcttttat ttgacagtgg agagacagcg
721 gaagcgactc gtctcaaacg gacagctcgt agaaggtata cacgtcggaa gaatcgtatt
781 tgttatctac aggagatttt ttcaaatgag atggcgaaag tagatgatag tttctttcat
841 cgacttgaag agtctttttt ggtggaagaa gacaagaagc atgaacgtca tcctattttt
901 ggaaatatag tagatgaagt tgcttatcat gagaaatatc caactatcta tcatctgcga
961 aaaaaattgg tagattctac tgataaagcg gatttgcgct taatctattt ggccttagcg
1021 catatgatta agtttcgtgg tcattttttg attgagggag atttaaatcc tgataatagt
1081 gatgtggaca aactatttat ccagttggta caaacctaca atcaattatt tgaagaaaac
1141 cctattaacg caagtggagt agatgctaaa gcgattcttt ctgcacgatt gagtaaatca
1201 agacgattag aaaatctcat tgctcagctc cccggtgaga agaaaaatgg cttatttggg
1261 aatctcattg ctttgtcatt gggtttgacc cctaatttta aatcaaattt tgatttggca
1321 gaagatgcta aattacagct ttcaaaagat acttacgatg atgatttaga taatttattg
1381 gcgcaaattg gagatcaata tgctgatttg tttttggcag ctaagaattt atcagatgct
1441 attttacttt cagatatcct aagagtaaat actgaaataa ctaaggctcc cctatcagct
1501 tcaatgatta aacgctacga tgaacatcat caagacttga ctcttttaaa agctttagtt
1561 cgacaacaac ttccagaaaa gtataaagaa atcttttttg atcaatcaaa aaacggatat
1621 gcaggttata ttgatggggg agctagccaa gaagaatttt ataaatttat caaaccaatt
1681 ttagaaaaaa tggatggtac tgaggaatta ttggtgaaac taaatcgtga agatttgctg
1741 cgcaagcaac ggacctttga caacggctct attccccatc aaattcactt gggtgagctg
1801 catgctattt tgagaagaca agaagacttt tatccatttt taaaagacaa tcgtgagaag
1861 attgaaaaaa tcttgacttt tcgaattcct tattatgttg gtccattggc gcgtggcaat
1921 agtcgttttg catggatgac tcggaagtct gaagaaacaa ttaccccatg gaattttgaa
1981 gaagttgtcg ataaaggtgc ttcagctcaa tcatttattg aacgcatgac aaactttgat
2041 aaaaatcttc caaatgaaaa agtactacca aaacatagtt tgctttatga gtattttacg
2101 gtttataacg aattgacaaa ggtcaaatat gttactgaag gaatgcgaaa accagcattt
2161 ctttcaggtg aacagaagaa agccattgtt gatttactct tcaaaacaaa tcgaaaagta
2221 accgttaagc aattaaaaga agattatttc aaaaaaatag aatgttttga tagtgttgaa
2281 atttcaggag ttgaagatag atttaatgct tcattaggta cctaccatga tttgctaaaa
2341 attattaaag ataaagattt tttggataat gaagaaaatg aagatatctt agaggatatt
2401 gttttaacat tgaccttatt tgaagatagg gagatgattg aggaaagact taaaacatat
2461 gctcacctct ttgatgataa ggtgatgaaa cagcttaaac gtcgccgtta tactggttgg
2521 ggacgtttgt ctcgaaaatt gattaatggt attagggata agcaatctgg caaaacaata
2581 ttagattttt tgaaatcaga tggttttgcc aatcgcaatt ttatgcagct gatccatgat
2641 gatagtttga catttaaaga agacattcaa aaagcacaag tgtctggaca aggcgatagt
2701 ttacatgaac atattgcaaa tttagctggt agccctgcta ttaaaaaagg tattttacag
2761 actgtaaaag ttgttgatga attggtcaaa gtaatggggc ggcataagcc agaaaatatc
2821 gttattgaaa tggcacgtga aaatcagaca actcaaaagg gccagaaaaa ttcgcgagag
2881 cgtatgaaac gaatcgaaga aggtatcaaa gaattaggaa gtcagattct taaagagcat
2941 cctgttgaaa atactcaatt gcaaaatgaa aagctctatc tctattatct ccaaaatgga
3001 agagacatgt atgtggacca agaattagat attaatcgtt taagtgatta tgatgtcgat
3061 cacattgttc cacaaagttt ccttaaagac gattcaatag acaataaggt cttaacgcgt
3121 tctgataaaa atcgtggtaa atcggataac gttccaagtg aagaagtagt caaaaagatg
3181 aaaaactatt ggagacaact tctaaacgcc aagttaatca ctcaacgtaa gtttgataat
3241 ttaacgaaag ctgaacgtgg aggtttgagt gaacttgata aagctggttt tatcaaacgc
3301 caattggttg aaactcgcca aatcactaag catgtggcac aaattttgga tagtcgcatg
3361 aatactaaat acgatgaaaa tgataaactt attcgagagg ttaaagtgat taccttaaaa
3421 tctaaattag tttctgactt ccgaaaagat ttccaattct ataaagtacg tgagattaac
3481 aattaccatc atgcccatga tgcgtatcta aatgccgtcg ttggaactgc tttgattaag
3541 aaatatccaa aacttgaatc ggagtttgtc tatggtgatt ataaagttta tgatgttcgt
3601 aaaatgattg ctaagtctga gcaagaaata ggcaaagcaa ccgcaaaata tttcttttac
3661 tctaatatca tgaacttctt caaaacagaa attacacttg caaatggaga gattcgcaaa
3721 cgccctctaa tcgaaactaa tggggaaact ggagaaattg tctgggataa agggcgagat
3781 tttgccacag tgcgcaaagt attgtccatg ccccaagtca atattgtcaa gaaaacagaa
3841 gtacagacag gcggattctc caaggagtca attttaccaa aaagaaattc ggacaagctt
3901 attgctcgta aaaaagactg ggatccaaaa aaatatggtg gttttgatag tccaacggta
3961 gcttattcag tcctagtggt tgctaaggtg gaaaaaggga aatcgaagaa gttaaaatcc
4021 gttaaagagt tactagggat cacaattatg gaaagaagtt cctttgaaaa aaatccgatt
4081 gactttttag aagctaaagg atataaggaa gttaaaaaag acttaatcat taaactacct
4141 aaatatagtc tttttgagtt agaaaacggt cgtaaacgga tgctggctag tgccggagaa
4201 ttacaaaaag gaaatgagct ggctctgcca agcaaatatg tgaatttttt atatttagct
4261 agtcattatg aaaagttgaa gggtagtcca gaagataacg aacaaaaaca attgtttgtg
4321 gagcagcata agcattattt agatgagatt attgagcaaa tcagtgaatt ttctaagcgt
4381 gttattttag cagatgccaa tttagataaa gttcttagtg catataacaa acatagagac
4441 aaaccaatac gtgaacaagc agaaaatatt attcatttat ttacgttgac gaatcttgga
4501 gctcccgctg cttttaaata ttttgataca acaattgatc gtaaacgata tacgtctaca
4561 aaagaagttt tagatgccac tcttatccat caatccatca ctggtcttta tgaaacacgc
4621 attgatttga gtcagctagg aggtgactga agtatatttt agatgaagat tatttcttaa
4681 taactaaaaa tatggtataa tactcttaat aaatgcagta atacaggggc ttttcaagac
4741 tgaagtctag ctgagacaaa tagtgcgatt acgaaatttt ttagacaaaa atagtctacg
4801 aggttttaga gctatgctgt tttgaatggt cccaaaactg agaccagtct cggaagctca
4861 aaggtctcgt tttagagcta tgctgttttg aatggtccca aaacttcagc acactgagac
4921 ttgttgagtt ccatgtttta gagctatgct gttttgaatg gactccattc aacattgccg
4981 atgataactt gagaaagagg gttaatacca gcagtcggat accttcctat tctttctgtt
5041 aaagcgtttt catgttataa taggcaaaag aagagtagtg tgatggatcc actagttcta
5101 gaatcgatac gattttgaag tggcaacaga taaaaaaaag cagtttaaaa ttgttgctga
5161 acttttaaaa caagcaaata caatcattgt cgcaacagat agcgacagag aaggcgaaaa
5221 cattgcctgg tcgatcattc ataaagcaaa tgccttttct aaagataaaa cgtataaaag
5281 actatggatc aatagtttag aaaaagatgt gatccgtagc ggttttcaaa atttgcgacc
5341 aggaatgaat tactatccct tttatcaaga agcgcacaaa aagaaaaacg aaatgataca
5401 ccaatcagtg caaaaaaaga tataatggga gataagacgg ttcgtgttcg tgctgacttg
5461 caccatatca taaaaatcga aacagcaaag aatggcggaa acgtaaaaga agttatggaa
5521 ataagactta gaagcaaact taagagtgtg ttgatagtgc agtatcttaa aattttgtat
5581 aataggaatt gaagttaaat tagatgctaa aaatttgtaa ttaagaagga gtgattacat
5641 gaactttaat aaaattgatt tagacaattg gaagagaaaa gagatattta atcattattt
5701 gaaccaacaa acgactttta gtataaccac agaaattgat attagtgttt tataccgaaa
5761 cataaaacaa gaaggatata aattttaccc tgcatttatt ttcttagtga caagggtgat
5821 aaactcaaat acagctttta gaactggtta caatagcgac ggagagttag gttattggga
5881 taagttagag ccactttata caatttttga tggtgtatct aaaacattct ctggtatttg
5941 gactcctgta aagaatgact tcaaagagtt ttatgattta tacctttctg atgtagagaa
6001 atataatggt tcggggaaat tgtttcccaa aacacctata cctgaaaatg ctttttctct
6061 ttctattatt ccatggactt catttactgg gtttaactta aatatcaata ataatagtaa
6121 ttaccttcta cccattatta cagcaggaaa attcattaat aaaggtaatt caatatattt
6181 accgctatct ttacaggtac atcattctgt ttgtgatggt tatcatgcag gattgtttat
6241 gaactctatt caggaattgt cagataggcc taatgactgg cttttataat tctatgagtc
6301 gcttttgtaa atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta
6361 tactactgac agcttccaag gagctaaaga gttgagatcc tttttttctg cgcgtaatat
6421 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
6481 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
6541 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
6601 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
6661 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
6721 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
6781 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
6841 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
6901 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
6961 gggggcggag cctatggaaa cctagcgctt agaatcgctt taggaaacac gatccagtcc
7021 aataatcgtc gataaaaact tttgaaaaag gttggtgaaa ttacctactt ttggaataat
7081 cacaaatcac aagtgattaa tcacaaatca caagtgatta atcacttgtt tattaagata
7141 ttaaaagcta taatttaaat aaagcgtgaa ttttattaca caaaaagagg ggggagaaac
7201 ttggaactag catttagaga aagcttaaaa aagatgagag gtaccaaatc aaaagaaaaa
7261 ttctcccaag aattagaaat gagtagatca aattattcac gaatagaatc aggaaaatca
7321 gatccaacca taaaaacact agaacaaatt gcaaagttaa ctaactcaac gctagtagtg
7381 gatttaatcc caaatgagcc aacagaacca gaaccagaaa cagaatcaga acaagtaaca
7441 ttggatttag aaatggaaga agaaaaaagc aatgacttcg tgtgaataat gcgcgaaatc
7501 gttgcttatt ttttttaaaa gcggtatact agatataacg aaacaacgaa ctgaatagaa
7561 acgaaaaaag agccatgaca catttataaa atgtttgacg acattttata aatgcatagc
7621 ccgataagat tgccaaacca acgcttatca gttagtcaga tgaactcttc cctcgtaaga
7681 agttatttaa ttaactttgt ttgaagacgg tatataaccg tactatcatt atatagggaa
7741 atcagagagt tttcaagtat ctaagctact gaatttaaga attgttaagc aatcaatcgg
7801 aaatcgtttg attgcttttt ttgtattcat ttatagaagg tggagtttgt atgaatcatg
7861 atgaatgtaa aacttatata aaaaatagtt tattggagat aagaaaatta gcaaatatct
7921 atacactaga aacgtttaag aaagagttag aaaagagaaa tatctactta gaaacaaaat
7981 cagataagta tttttcttcg gagggggaag attatatata taagttaata gaaaataaca
8041 aaataattta ttcgattagt ggaaaaaaat tgacttataa aggaaaaaaa tctttttcaa
8101 aacatgcaat attgaaacag ttgaatgaaa aagcaaacca agttaattaa acaacctatt
8161 ttataggatt tataggaaag gagaacagct gaatgaatat cccttttgtt gtagaaactg
8221 tgcttcatga cggcttgtta aagtacaaat ttaaaaatag taaaattcgc tcaatcacta
8281 ccaagccagg taaaagcaaa ggggctattt ttgcgtatcg ctcaaaatca agcatgattg
8341 gcggtcgtgg tgttgttctg acttccgagg aagcgattca agaaaatcaa gatacattta
8401 cacattggac acccaacgtt tatcgttatg gaacgtatgc agacgaaaac cgttcataca
8461 cgaaaggaca ttctgaaaac aatttaagac aaatcaatac cttctttatt gattttgata
8521 ttcacacggc aaaagaaact atttcagcaa gcgatatttt aacaaccgct attgatttag
8581 gttttatgcc tactatgatt atcaaatctg ataaaggtta tcaagcatat tttgttttag
8641 aaacgccagt ctatgtgact tcaaaatcag aatttaaatc tgtcaaagca gccaaaataa
8701 tttcgcaaaa tatccgagaa tattttggaa agtctttgcc agttgatcta acgtgtaatc
8761 attttggtat tgctcgcata ccaagaacgg acaatgtaga attttttgat cctaattacc
8821 gttattcttt caaagaatgg caagattggt ctttcaaaca aacagataat aagggcttta
8881 ctcgttcaag tctaacggtt ttaagcggta cagaaggcaa aaaacaagta gatgaaccct
8941 ggtttaatct cttattgcac gaaacgaaat tttcaggaga aaagggttta atagggcgta
9001 ataacgtcat gtttaccctc tctttagcct actttagttc aggctattca atcgaaacgt
9061 gcgaatataa tatgtttgag tttaataatc gattagatca acccttagaa gaaaaagaag
9121 taatcaaaat tgttagaagt gcctattcag aaaactatca aggggctaat agggaataca
9181 ttaccattct ttgcaaagct tgggtatcaa gtgatttaac cagtaaagat ttatttgtcc
9241 gtcaagggtg gtttaaattc aagaaaaaaa gaagcgaacg tcaacgtgtt catttgtcag
9301 aatggaaaga agatttaatg gcttatatta gcgaaaaaag cgatgtatac aagccttatt
9361 tagtgacgac caaaaaagag attagagaag tgctaggcat tcctgaacgg acattagata
9421 aattgctgaa ggtactgaag gcgaatcagg aaattttctt taagattaaa ccaggaagaa
9481 atggtggcat tcaacttgct agtgttaaat cattgttgct atcgatcatt aaagtaaaaa
9541 aagaagaaaa agaaagctat ataaaggcgc tgacaaattc ttttgactta gagcatacat
9601 tcattcaaga gactttaaac aagctagcag aacgccctaa aacggacaca caactcgatt
9661 tgtttagcta tgatacaggc tgaaaataaa acccgcacta tgccattaca tttatatcta
9721 tgatacgtgt ttgttttttc tttgctgttt agcgaatgat tagcagaaat atacagagta
9781 agattttaat taattattag ggggagaagg agagagtagc ccgaaaactt ttagttggct
9841 tggactgaac gaagtgaggg aaaggctact aaaacgtcga ggggcagtga gagcgaagcg
9901 aacacttgat tttttaattt tctatctttt ataggtcatt agagtatact tatttgtcct
9961 ataaactatt tagcagcata atagatttat tgaataggtc atttaagttg agcatattag
10021 aggaggaaaa tcttggagaa atatttgaag aacccgatta catggattgg attagttctt
10081 gtggttacgt ggtttttaac taaaagtagt gaatttttga tttttggtgt gtgtgtcttg
10141 ttgttagtat ttgctagtca aagtgattaa atagaattct catgtttgac agcttatcat
10201 cggagctccg atgataagct gtcaaacatg agaattcccg gggatcc
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
eSpCas9-LentiCRISPR v2
2020-02-14
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