pSIN4-EF2-ABCG2-IRES-Neo

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: pSIN4-EF2-ABCG2-IRES-Neo
出品公司: Addgene
目录编号: 25983
存储实验室: Ren-he Xu

载体骨架基本信息
载体名称: pSIN-EF2-MCS-IRES-Neo
载体出品公司: James Thomson
空载体大小: 6500 bp
完整质粒大小: 9576 bp
载体修饰: --
载体类型: 哺乳动物表达,慢病毒
筛选标记: 新霉素G418筛选)
载体抗性: 氨苄青霉素,100μg/mL
生长温度: 37 ℃
宿主菌: Stbl3
拷贝数: 高拷贝

插入基因信息
插入基因名称: ATP-binding cassette, sub-family G (WHITE), member 2, variant of isoform 2
物种来源: H. sapiens (human)
插入大小: 1968 bp
基因突变: --
GenBank编号 AAH92408.1
启动子: --
标签/融合蛋白: EF1 apha启动子(骨架上的N末端)IRES Neo(主干网上的C终端)
克隆方法: 限制性内切酶
5’克隆位点: EcoR I(未知是否破坏)
3’克隆位点: BamH I (未知是否破坏)
5’测序引物 EF-1a-F
3’测序引物 IRES-R

文献引用方法
材料和方法部分: pSIN4-EF2-ABCG2-IRES-Neo was a gift from Ren-he Xu (Addgene plasmid # 25983 ; http://n2t.net/addgene:25983 ; RRID:Addgene_25983)
参考文献部分: Lack of ABCG2 expression and side population properties in human pluripotent stem cells. Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ, Chen FP, Xu RH. Stem Cells. 2009 Oct . 27(10):2435-45. 10.1002/stem.192 PubMed 19670287

载体图谱质粒图谱和多克隆位点信息

pSIN4-EF2-ABCG2-IRES-Neo质粒图谱

载体简介

载体序列

LOCUS       pSIN4-EF2-ABCG2-IRES-Neo                9576 bp ds-DNA     circular SYN 16-JUN-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    pSIN4-EF2-ABCG2-IRES-Neo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9576)
  AUTHORS   Zeng H, Park JW, Guo M, Lin G, Crandall L, Compton T, Wang X, Li XJ,
            Chen FP, Xu RH
  TITLE     Lack of ABCG2 expression and side population properties in human 
            pluripotent stem cells.
  JOURNAL   Stem Cells. 2009 Oct . 27(10):2435-45.
  PUBMED    19670287
REFERENCE   2  (bases 1 to 9576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jun 16, 2022 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9576
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     primer_bind     complement(159..176)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     386..405
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             601..1404
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(664..683)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1274..1293
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     LTR             1607..1840
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(1991..2008)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    1997..2221
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     regulatory      2264..2273
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     promoter        2317..2421
                     /gene="bla"
                     /label=AmpR promoter
     CDS             2422..3282
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2640..2659)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3453..4041
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3942..3961
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     LTR             4200..4833
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4880..5005
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5498..5731
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5916..5960
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6247..6364
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        6493..7671
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6724..7662
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7619..7639
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    9568..565
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
ORIGIN
        1 ctcccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt
       61 ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg
      121 ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg
      181 tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc
      241 tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca
      301 aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag
      361 ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa
      421 ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt
      481 tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt
      541 tttcctttga aaaacacgat gataagcttg ccacaacccg ggataattcc tgcagccaat
      601 atgggatcgg ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
      661 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
      721 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
      781 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
      841 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
      901 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
      961 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     1021 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     1081 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     1141 atgcccgacg gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     1201 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     1261 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     1321 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     1381 cgccttcttg acgagttctt ctgataatag gcggccgctc gagacctaga aaaacatgga
     1441 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     1501 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     1561 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
     1621 cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
     1681 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
     1741 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
     1801 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
     1861 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat
     1921 tataatagat ttagcaggaa ttgaactagg agtggagcac acaggcaaag ttctagagct
     1981 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     2041 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     2101 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     2161 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     2221 gcttctgagg cggaaagaac cagctggggg cgcgcccctc gaggccgcca tggtcatagc
     2281 tgtttgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     2341 ttctaaatac attcaaatat gtatccgctc acgagacaat aaccctgata aatgcttcaa
     2401 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     2461 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     2521 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     2581 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     2641 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     2701 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     2761 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     2821 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     2881 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     2941 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     3001 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     3061 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     3121 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     3181 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     3241 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     3301 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     3361 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     3421 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3481 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3541 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3601 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3661 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3721 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3781 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     3841 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     3901 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     3961 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4021 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt ctggcctttt
     4081 gcacggaccg ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     4141 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     4201 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     4261 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     4321 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     4381 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     4441 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     4501 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     4561 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     4621 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     4681 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     4741 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     4801 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     4861 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     4921 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     4981 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     5041 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     5101 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     5161 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     5221 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     5281 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     5341 agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat
     5401 tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga
     5461 gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct
     5521 tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac
     5581 aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac
     5641 agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg
     5701 tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca
     5761 tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt
     5821 ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac
     5881 actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat
     5941 tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa
     6001 aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt
     6061 ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa
     6121 ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag
     6181 acagatccat tcgattagtg aacggatctc gacggtatcg ccacaaatgg cagtattcat
     6241 ccacaatttt aaaagaaagg gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     6301 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     6361 ttttcgggtt tattacaggg acagcagaga tccactttgg atcgataagc tttgcaaaga
     6421 tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa
     6481 ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc
     6541 gagaagttgg ggggaggggt cggcaattga accggtgcct agagaaggtg gcgcggggta
     6601 aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg gggagaaccg
     6661 tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca
     6721 caggtaagtg ccgtgtgtgg ttcccgcggg cctggcctct ttacgggtta tggcccttgc
     6781 gtgccttgaa ttacttccac ctggctgcag tacgtgattc ttgatcccga gcttcgggtt
     6841 ggaagtgggt gggagagttc gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga
     6901 gttgaggcct ggcctgggcg ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc
     6961 tgtctcgctg ctttcgataa gtctctagcc atttaaaatt tttgatgacc tgctgcgacg
     7021 ctttttttct ggcaagatag tcttgtaaat gcgggccaag atctgcacac tggtatttcg
     7081 gtttttgggg ccgcgggcgg cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg
     7141 cggggcctgc gagcgcggcc accgagaatc ggacgggggt agtctcaagc tggccggcct
     7201 gctctggtgc ctggcctcgc gccgccgtgt atcgccccgc cctgggcggc aaggctggcc
     7261 cggtcggcac cagttgcgtg agcggaaaga tggccgcttc ccggccctgc tgcagggagc
     7321 tcaaaatgga ggacgcggcg ctcgggagag cgggcgggtg agtcacccac acaaaggaaa
     7381 agggcctttc cgtcctcagc cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc
     7441 aggcacctcg attagttctc gagcttttgg agtacgtcgt ctttaggttg gggggagggg
     7501 ttttatgcga tggagtttcc ccacactgag tgggtggaga ctgaagttag gccagcttgg
     7561 cacttgatgt aattctcctt ggaatttgcc ctttttgagt ttggatcttg gttcattctc
     7621 aagcctcaga cagtggttca aagttttttt cttccatttc aggtgtcgtg aggaattcgc
     7681 caccatgtct tccagtaatg tcgaagtttt tatcccagtg tcacaaggaa acaccaatgg
     7741 cttccccgcg acagcttcca atgacctgaa ggcatttact gaaggagctg tgttaagttt
     7801 tcataacatc tgctatcgag taaaactgaa gagtggcttt ctaccttgtc gaaaaccagt
     7861 tgagaaagaa atattatcga atatcaatgg gatcatgaaa cctggtctca acgccatcct
     7921 gggacccaca ggtggaggca aatcttcgtt attagatgtc ttagctgcaa ggaaagatcc
     7981 aagtggatta tctggagatg ttctgataaa tggagcaccg cgacctgcca atttcaaatg
     8041 taattcaggt tacgtggtac aagatgatgt tgtgatgggc actctgacgg tgagagaaaa
     8101 cttaaagttc tcagcagctc ttcggcttgc aacaactatg acgaatcatg aaaaaaacga
     8161 acggattaac agggtcattc aagagttagg tctggataaa gtggcagact ccaaggttgg
     8221 aactcagttt atccgtggtg tgtctggagg agaaagaaaa aggactagta taggaatgga
     8281 gcttatcact gatccttcca tcttgttctt ggatgagcct acaactggct tagactcaag
     8341 cacagcaaat gctgtccttt tgctcctgaa aaggatgtct aagcagggac gaacaatcat
     8401 cttctccatt catcagcctc gatattccat cttcaagttg tttgatagcc tcaccttatt
     8461 ggcctcagga agacttatgt tccacgggcc tgctcaggag gccttgggat actttgaatc
     8521 agctggttat cactgtgagg cctataataa ccctgcagac ttcttcttgg acatcattaa
     8581 tggagattcc actgctgtgg cattaaacag agaagaagac tttaaagcca cagagatcat
     8641 agagccttcc aagcaggata agccactcat agaaaaatta gcggagattt atgtcaactc
     8701 ctccttctac aaagagacaa aagctgaatt acatcaactt tccgggggtg agaagaagaa
     8761 gaagatcaca gtcttcaagg agatcagcta caccacctcc ttctgtcatc aactcagatg
     8821 ggtttccaag cgttcattca aaaacttgct ggttaatccc caggcctcta tagctcagat
     8881 cattgtcaca gtcgtactgg gactggttat aggtgccatt tactttgggc taaaaaatga
     8941 ttctactgga atccagaaca gagctggggt tctcttcttc ctgacgacca accagtgttt
     9001 cagcagtgtt tcagccgtgg aactctttgt ggtagagaag aagctcttca tacatgaata
     9061 catcagcgga tactacagag tgtcatctta tttccttgga aaactgttat ctgatttatt
     9121 acccatgagg atgttaccaa gtattatatt tacctgtata gtgtacttca tgttaggatt
     9181 gaagccaaag gcagatgcct tcttcgttat gatgtttacc cttatgatgg tggcttattc
     9241 agccagttcc atggcactgg ccatagcagc aggtcagagt gtggtttctg tagcaacact
     9301 tctcatgacc atctgttttg tgtttatgat ggtctgttgg tcaatctcac aaccattgca
     9361 tcttggctgt catggcttca gtacttcagc attccacgat atggatttac ggctttgcag
     9421 cataatgaat ttttgggaca aaacttctgc ccaggactca atgcaacagg aaacaatcct
     9481 tgtaactatg caacatgtac tggcgaagaa tatttggtaa ggatccgcat gcatctaggg
     9541 cggccaattc cgcccctctc cccccccccc ctctcc
//

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....