| 质粒名称: | Myc-TEAD4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 24638 |
| 存储实验室: | Kunliang Guan |
|
载体骨架基本信息 |
|
| 载体名称: | pRK5-Myc |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TEAD-4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1083 bp |
| 基因突变: | -- |
| GenBank编号 | EAW88873.1 |
| 启动子 | -- |
| 标签/融合蛋白: | Myc（插入件上的N端子） |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | Myc-TEAD4 was a gift from Kunliang Guan (Addgene plasmid # 24638 ; http://n2t.net/addgene:24638 ; RRID:Addgene_24638) |
| 参考文献部分: | Structural insights into the YAP and TEAD complex. Li Z, Zhao B, Wang P, Chen F, Dong Z, Yang H, Guan KL, Xu Y. Genes Dev. 2010 Feb 1;24(3):235-40. 10.1101/gad.1865810 PubMed 20123905 |
买家导航
MycTEAD4载体基本信息
MycTEAD4载体图谱质粒图谱和多克隆位点信息
MycTEAD4载体简介
MycTEAD4载体序列
LOCUS Myc-TEAD4 5773 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Myc-TEAD4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5773)
AUTHORS Li Z, Zhao B, Wang P, Chen F, Dong Z, Yang H, Guan KL, Xu Y
TITLE Structural insights into the YAP and TEAD complex.
JOURNAL Genes Dev. 2010 Feb 1;24(3):235-40.
PUBMED 20123905
REFERENCE 2 (bases 1 to 5773)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5773
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 55..76
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 91..121
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 129..145
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 134..156
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 153..169
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 153..169
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
enhancer 198..577
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 578..781
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 732..752
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 778..802
/label=LNCX
/note="Human CMV promoter, forward primer"
promoter 1012..1030
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind 1012..1029
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind 1043..1062
/label=pMT2-F
/note="Synthetic intron, forward primer"
CDS 1111..1140
/codon_start=1
/product="Myc (human c-Myc proto-oncogene) epitope tag"
/label=Myc
/translation="EQKLISEEDL"
regulatory 2257..2266
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
polyA_signal 2270..2404
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(2307..2326)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 2361..2380
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
promoter 2473..2830
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2496..2516)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2681..2816
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2743..2762
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
primer_bind complement(2850..2867)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(2850..2866)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2859..2881)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 3079..3534
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(3166..3185)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 3376..3397
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(3551..3570)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 3670..3692
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(3730..3748)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 3816..3920
/gene="bla"
/label=AmpR promoter
CDS 3921..4781
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(4139..4158)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 4952..5540
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5441..5460
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 5694..5711
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
61 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
121 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
181 ttaattcgag ctcgcccgac attgattatt gactagttat taatagtaat caattacggg
241 gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc
301 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
361 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
421 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
481 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
541 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
601 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
661 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
721 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
781 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
841 aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa cgcggattcc
901 ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc ccttggcttc
961 gttagaacgc ggctacaatt aatacataac cttatgtatc atacacatac gatttaggtg
1021 acactataga ataacatcca ctttgccttt ctctccacag gtgtccactc ccaggtccaa
1081 ctgcacctcg gttctatcga tcccaccatg gagcagaagc tgatcagcga ggaggacctg
1141 ctcgagggat cctatggtcg gaacgagctg attgcccgct acatcaagct ccggacaggg
1201 aagacccgca ccaggaagca ggtctccagc cacatccagg tgctggctcg tcgcaaagct
1261 cgcgagatcc aggccaagct aaaggaccag gcagctaagg acaaggccct gcagagcatg
1321 gctgccatgt cgtctgcaca gatcatctcc gccacggcct tccacagtag catggccctc
1381 gcccggggcc ccggccgccc agcagtctca gggttttggc aaggagcttt gccaggccaa
1441 gccggaacgt cccatgatgt gaagcctttc tctcagcaaa cctatgctgt ccagcctccg
1501 ctgcctctgc cagggtttga gtctcctgca gggcccgccc catcgccctc tgcgcccccg
1561 gcacccccat ggcagggccg cagcgtggcc agctccaagc tctggatgtt ggagttctct
1621 gccttcctgg agcagcagca ggacccggac acgtacaaca agcacctgtt cgtgcacatt
1681 ggccagtcca gcccaagcta cagcgacccc tacctcgaag ccgtggacat ccgccaaatc
1741 tatgacaaat tcccggagaa aaagggtgga ctcaaggatc tcttcgaacg gggaccctcc
1801 aatgcctttt ttcttgtgaa gttctgggca gacctcaaca ccaacatcga ggatgaaggc
1861 agctccttct atggggtctc cagccagtat gagagccccg agaacatgat catcacctgc
1921 tccacgaagg tctgctcttt cggcaagcag gtggtggaga aagttgagac agagtatgct
1981 cgctatgaga atggacacta ctcttaccgc atccaccggt ccccgctctg tgagtacatg
2041 atcaacttca tccacaagct caagcacctc cctgagaagt acatgatgaa cagcgtgctg
2101 gagaacttca ccatcctgca ggtggtcacc aacagagaca cacaggagac cttgctgtgc
2161 attgcctatg tctttgaggt gtcagccagt gagcacgggg ctcagcacca catctacagg
2221 ctggtgaaag aatgagaatt ctagattaga agcttggccg ccatggccca acttgtttat
2281 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
2341 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
2401 gatcgggaat taattcggcg cagcaccatg gcctgaaata acctctgaaa gaggaacttg
2461 gttaggtacc ttctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
2521 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
2581 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2641 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
2701 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
2761 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
2821 cttttgcaaa aagctgttaa cagcttggca ctggccgtcg ttttacaacg tcgtgactgg
2881 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
2941 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
3001 gaatggcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
3061 cgtcaaagca accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
3121 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
3181 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
3241 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
3301 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
3361 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg
3421 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
3481 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt
3541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
3601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
3661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
3721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
3781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
3901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
3961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
4021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
4081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
4141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
4201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
4261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
4321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
5701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
5761 cgattcatta atg
//
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