| 质粒名称: | pJC175e |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 79219 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | SC101 |
| 载体出品公司: | David Liu Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2992 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | PPSP gIII, luxAB |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | PPSP |
| 标签/融合蛋白: | -- |
| 克隆方法: | Ligation Independent克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pJC175e was a gift from David Liu (Addgene plasmid # 79219 ; http://n2t.net/addgene:79219 ; RRID:Addgene_79219) |
| 参考文献部分: | Continuous evolution of Bacillus thuringiensis toxins overcomes insect resistance. Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR. Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub 2016 Apr 27. 10.1038/nature17938 PubMed 27120167 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pJC175e 6527 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Directed Evolution of Protein-Protein Interactions.
ACCESSION .
VERSION .
KEYWORDS pJC175e
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6527)
AUTHORS Badran AH, Guzov VM, Huai Q, Kemp MM, Vishwanath P, Kain W, Nance
AM, Evdokimov A, Moshiri F, Turner KH, Wang P, Malvar T, Liu DR
TITLE Continuous evolution of Bacillus thuringiensis toxins overcomes
insect resistance.
JOURNAL Nature. 2016 May 5;533(7601):58-63. doi: 10.1038/nature17938. Epub
2016 Apr 27.
PUBMED 27120167
REFERENCE 2 (bases 1 to 6527)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6527
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS complement(1..951)
/codon_start=1
/gene="rep101"
/product="RepA protein needed for replication with the
pSC101 origin"
/label=Rep101
/translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
rep_origin 999..1221
/label=pSC101 ori
/note="low-copy replication origin that requires the Rep101
protein"
CDS 3253..4332
/codon_start=1
/gene="luxA"
/product="LuxA luciferase subunit"
/label=LuxA
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
KLFQSDVMPFLKEKQRSLLY"
CDS 4396..5376
/codon_start=1
/gene="luxB"
/product="LuxB luciferase subunit"
/label=LuxB
/note="from the Photorhabdus luminescens bioluminescence
operon"
/translation="KFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENHF
SDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVRIAEEACLLDQLSEGRFILGF
SDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAYT
PGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEID
HQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECITA
AKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHTEYT"
CDS complement(5456..6316)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6079..6098
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6317..6421)
/gene="bla"
/label=AmpR promoter
primer_bind 6489..6507
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
ORIGIN
1 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
61 ccatgagaac gaaccattga gatcatgctt actttgcatg tcactcaaaa attttgcctc
121 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
181 acgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
241 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
301 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
361 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
421 ttcaagcatt aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt
481 tttcttttgt gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc
541 aaaagactta acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg
601 caatatctct tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt
661 ccactggaaa atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat
721 cagctctctg gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat
781 ctgagcgtat tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat
841 cgtggggttg agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc
901 atagcgacta atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa
961 ttggtctagg tgattttaat cactatacca attgagatgg gctagtcaat gataattact
1021 agtccttttc ctttgagttg tgggtatctg taaattctgc tagacctttg ctggaaaact
1081 tgtaaattct gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta
1141 tattcaagtg gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga
1201 tcccagccct gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc
1261 gcaaacgctg tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac
1321 cctcgcaagc tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga
1381 gtcgctgtct ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg
1441 gtaaatggca ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag
1501 aaaagcccgt cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct
1561 gactttttgc tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta
1621 tcccgtgaca ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca
1681 ggcttacccg tcttactgtc aagaggacat ccggtcaaat aaaacgaaag gctcagtcga
1741 aagactgggc ctttcgtttt gctgaggaga cttagggacc ctgatgaaat tcgccacttg
1801 ttagtgtaat tcgctaactc atcctggcat gttgctgttg attcttcaat cagatcttta
1861 taaatcaaaa agataaaaaa ttggcacgca aattgtatta acagttcagc aggacaatcc
1921 tgaacgcaga aatcaagagg acaacattac ctgcaggtgc agtaaggagg aaaaaaaaat
1981 gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc tattctcact ccgctgaaac
2041 tgttgaaagt tgtttagcaa aaccccatac agaaaattca tttactaacg tctggaaaga
2101 cgacaaaact ttagatcgtt acgctaacta tgagggctgt ctgtggaatg ctacaggcgt
2161 tgtagtttgt actggtgacg aaactcagtg ttacggtaca tgggttccta ttgggcttgc
2221 tatccctgaa aatgagggtg gtggctctga gggtggcggt tctgagggtg gcggttctga
2281 gggtggcggt actaaacctc ctgagtacgg tgatacacct attccgggct atacttatat
2341 caaccctctc gacggcactt atccgcctgg tactgagcaa aaccccgcta atcctaatcc
2401 ttctcttgag gagtctcagc ctcttaatac tttcatgttt cagaataata ggttccgaaa
2461 taggcagggg gcattaactg tttatacggg cactgttact caaggcactg accccgttaa
2521 aacttattac cagtacactc ctgtatcatc aaaagccatg tatgacgctt actggaacgg
2581 taaattcaga gactgcgctt tccattctgg ctttaatgag gatccattcg tttgtgaata
2641 tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat gctggcggcg gctctggtgg
2701 tggttctggt ggcggctctg agggtggtgg ctctgagggt ggcggttctg agggtggcgg
2761 ctctgaggga ggcggttccg gtggtggctc tggttccggt gattttgatt atgaaaagat
2821 ggcaaacgct aataaggggg ctatgaccga aaatgccgat gaaaacgcgc tacagtctga
2881 cgctaaaggc aaacttgatt ctgtcgctac tgattacggt gctgctatcg atggtttcat
2941 tggtgacgtt tccggccttg ctaatggtaa tggtgctact ggtgattttg ctggctctaa
3001 ttcccaaatg gctcaagtcg gtgacggtga taattcacct ttaatgaata atttccgtca
3061 atatttacct tccctccctc aatcggttga atgtcgccct tttgtctttg gcgctggtaa
3121 accttacgag ttcagtatcg actgcgataa gatcaacctg ttccgcggtg tctttgcgtt
3181 tcttttatat gttgccacct ttatgtatgt attttctacg tttgctaaca tactgcgtaa
3241 taaggagtct taatgaaatt tggaaacttt ttgcttacat accaacctcc ccaattttcc
3301 caaacagagg taatgaaacg tttggttaaa ttaggtcgca tctctgagga gtgtggtttt
3361 gataccgtat ggttactgga gcatcatttc acggagtttg gtttgcttgg taacccttat
3421 gtcgctgctg catatttact tggcgcgact aaaaaattga atgtaggaac tgccgctatt
3481 gttcttccca cagcccatcc agtacgccaa cttgaagatg tgaatttatt ggatcaaatg
3541 tcaaaaggac gatttcggtt tggtatttgc cgagggcttt acaacaagga ctttcgcgta
3601 ttcggcacag atatgaataa cagtcgcgcc ttagcggaat gctggtacgg gctgataaag
3661 aatggcatga cagagggata tatggaagct gataatgaac atatcaagtt ccataaggta
3721 aaagtaaacc ccgcggcgta tagcagaggt ggcgcaccgg tttatgtggt ggctgaatca
3781 gcttcgacga ctgagtgggc tgctcaattt ggcctaccga tgatattaag ttggattata
3841 aatactaacg aaaagaaagc acaacttgag ctttataatg aagtggctca agaatatggg
3901 cacgatattc ataatatcga ccattgctta tcatatataa catctgtaga tcatgactca
3961 attaaagcga aagagatttg ccggaaattt ctggggcatt ggtatgattc ttatgtgaat
4021 gctacgacta tttttgatga ttcagaccaa acaagaggtt atgatttcaa taaagggcag
4081 tggcgtgact ttgtattaaa aggacataaa gatactaatc gccgtattga ttacagttac
4141 gaaatcaatc ccgtgggaac gccgcaggaa tgtattgaca taattcaaaa agacattgat
4201 gctacaggaa tatcaaatat ttgttgtgga tttgaagcta atggaacagt agacgaaatt
4261 attgcttcca tgaagctctt ccagtctgat gtcatgccat ttcttaaaga aaaacaacgt
4321 tcgctattat attatggcgg tggcggtagc ggcggtggcg gtagcggcgg tggcggtagc
4381 ggcggtggcg gtagcaaatt tggattgttc ttccttaact tcatcaattc aacaactgtt
4441 caagaacaga gtatagttcg catgcaggaa ataacggagt atgttgataa gttgaatttt
4501 gaacagattt tagtgtatga aaatcatttt tcagataatg gtgttgtcgg cgctcctctg
4561 actgtttctg gttttctgct cggtttaaca gagaaaatta aaattggttc attaaatcac
4621 atcattacaa ctcatcatcc tgtccgcata gcggaggaag cttgcttatt ggatcagtta
4681 agtgaaggga gatttatttt agggtttagt gattgcgaaa aaaaagatga aatgcatttt
4741 tttaatcgcc cggttgaata tcaacagcaa ctatttgaag agtgttatga aatcattaac
4801 gatgctttaa caacaggcta ttgtaatcca gataacgatt tttatagctt ccctaaaata
4861 tctgtaaatc cccatgctta tacgccaggc ggacctcgga aatatgtaac agcaaccagt
4921 catcatattg ttgagtgggc ggccaaaaaa ggtattcctc tcatctttaa gtgggatgat
4981 tctaatgatg ttagatatga atatgctgaa agatataaag ccgttgcgga taaatatgac
5041 gttgacctat cagagataga ccatcagtta atgatattag ttaactataa cgaagatagt
5101 aataaagcta aacaagagac gcgtgcattt attagtgatt atgttcttga aatgcaccct
5161 aatgaaaatt tcgaaaataa acttgaagaa ataattgcag aaaacgctgt cggaaattat
5221 acggagtgta taactgcggc taagttggca attgaaaagt gtggtgcgaa aagtgtattg
5281 ctgtcctttg aaccaatgaa tgatttgatg agccaaaaaa atgtaatcaa tattgttgat
5341 gataatatta agaagtacca cacggaatat acctaaactt aattaacggc actcctcagc
5401 aaatataatg accctcttga taacccaaga gggcattttt taatgcccat ggcgtttacc
5461 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
5521 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
5581 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
5641 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
5701 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
5761 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
5821 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
5881 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
5941 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
6001 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
6061 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
6121 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
6181 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
6241 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
6301 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
6361 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
6421 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
6481 cctataaaaa taggcgtatc acgaggccct taggccaaat aggccgt
//
