| 出品公司: | EMD Biosciences (Novagen) |
|---|---|
| 别名: | pET30b, pet 30b |
| 质粒类型: | 大肠杆菌蛋白表达 |
| 表达水平: | 高 |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 5421 bp |
| 5' 测序引物: | T7 |
| 5' 测序引物序列: | 5'-TAATACGACTCACTATAGGG-3' |
| 载体标签: | N-His, N-S, N-Thrombin, N-EK, C-His |
| 载体抗性: | Kanamycin |
| 备注: |
N term thrombin cleavage site; N term enterokinase cleavage site; a,b,c vary by MCS |
| 产品目录号: | 69910-3 |
| 稳定性: | 瞬时表达 Transient |
| 组成型: | 组成型 Constitutive |
| 病毒/非病毒: | 非病毒 |
| 表达宿主菌: | BL21(DE3) 、BLR(DE3)、Rosetta(DE3)、OrigamiB(DE3)、BL21(DE3)pLysS |
| pET表达手册： |
pET表达手册英文版 pET表达手册中文版 |
| pET载体汇总: |
pET-3a pET-11a pET-12a pET-14b pET-15b pET-16b pET-17b pET-19b pET-20b pET-21a pET-22b pET-23a pET-24a pET-25b pET-26b pET-27b pET-28a pET-29a pET-30a pET-31b pET-32a pET-33b pET-34b pET-35b pET-37b pET-39b pET-40b pET-41a pET-42a pET-43.1a pET-44a pET-45b pET-47b pET-48b pET-49b pET-50b pET-51b pET-52b pETDuet-1 pACYCDuet-1 pCDFDuet-1 pRSFDuet-1 pColaDuet-1 |
买家导航
pET30b载体基本信息
pET30b载体图谱质粒图谱和多克隆位点信息
pET30b载体简介
pET-30a-c系列载体携带有一个N端的His标签/Thrombin酶切位点/ S标签/ EK蛋白酶切标签以及一个可选择的C端His标签。
载体的多克隆位点见上面的图谱。需要注意的是图谱中的核苷酸编码顺序是以pBR322载体的复制方向为正方向，所以T7表达区域在图谱中是处于反向的位 置。T7 RNA聚合酶启动的克隆和表达区域在质粒图谱中也被标注了出来。质粒的F1复制子是被定向的，所以在T7噬菌体聚合酶的作用下，包含有蛋白编码序列的病毒 粒子能够产生，并启动蛋白表达，同时蛋白表达将被T7终止子序列(Cat. No. 69337-3)的作用下终止蛋白翻译。
pET30b载体序列
LOCUS pET-30b (+) 5421 bp DNA circular SYN
DEFINITION pET-30b (+)
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
COMMENT This file is created by Vector NTI
http://www.biofeng.com/
COMMENT ORIGDB|GenBank
COMMENT VNTAUTHORNAME|biofeng.com|
COMMENT VNTOAUTHORAD1|Novagen Inc.|
FEATURES Location/Qualifiers
source 1..5421
/organism="pET-30 b (+)"
/mol_type="other DNA"
terminator complement(1..129)
/label="T7_terminator"
misc_feature 69..87
/label="T7_Terminal_primer"
misc_feature complement(218..229)
/label="EK"
misc_feature 301..1907
/label="lacI"
CDS 326..1075
/label="ORF frame 2"
misc_feature complement(355..371)
/label="T7_transl_en_RBS"
misc_feature complement(389..416)
/label="lacO"
promoter complement(416..434)
/label="T7_promoter"
gene 470..733
/label="tet (300 - 563)"
/gene="tet (300 - 563)"
misc_feature complement(522..541)
/label="pBRrevBam_primer"
CDS 948..1907
/label="ORF frame 3"
gene 1966..2241
/label="tet (576 - 851)"
/gene="tet (576 - 851)"
misc_feature 2716..2907
/label="ROP"
misc_feature complement(2901..2923)
/label="pGEX_3_primer"
rep_origin complement(3322..3941)
/label="pBR322_origin"
gene 4047..4862
/label="KanR2"
/gene="KanR2"
CDS 4047..4862
/label="ORF frame 3"
ORIGIN
1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa
61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt
121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt
181 cgacggagct cgaattcgga tccgatatcg ccatggcctt gtcgtcgtcg tcggtaccca
241 gatctgggct gtccatgtgc tggcgttcga atttagcagc agcggtttct ttcataccag
301 aaccgcgtgg caccagacca gaagaatgat gatgatgatg gtgcatatgt atatctcctt
361 cttaaagtta aacaaaatta tttctagagg ggaattgtta tccgctcaca attcccctat
421 agtgagtcgt attaatttcg cgggatcgag atcgatctcg atcctctacg ccggacgcat
481 cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg ccgacatcac
541 cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg gcgtgggtat
601 ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg caccattcct
661 tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa tgcaggagtc
721 gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc tttcgcggta
781 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
841 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
901 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
961 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
1021 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
1081 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
1141 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
1201 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
1261 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
1321 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
1381 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
1441 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
1501 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
1561 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
1621 cggacatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
1681 taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
1741 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
1801 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
1861 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
1921 aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt gagagccttc
1981 aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc acttatgact
2041 gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt cattttcggc
2101 gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt attcggaatc
2161 ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt cggcgagaag
2221 caggccatta tcgccggcat ggcggcccca cgggtgcgca tgatcgtgct cctgtcgttg
2281 aggacccggc taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc
2341 gagcgaacgt gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg
2401 gtcttcggtt tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt
2461 atgttccgga tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta
2521 ttaacgaagc gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc
2581 gccagttgtt taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt
2641 atcgtgagca tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct
2701 tacacggagg catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc
2761 agaagccaga cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca
2821 gacatctgtg aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt
2881 ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt
2941 ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg
3001 tgtcggggcg cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact
3061 atgcggcatc agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca
3121 cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc
3181 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
3241 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
3301 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
3361 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
3421 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
3481 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
3541 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
3601 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
3661 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
3721 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac
3781 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
3841 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
3901 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
3961 tcagtggaac gaaaactcac gttaagggat tttggtcatg aacaataaaa ctgtctgctt
4021 acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg tcttgctcta
4081 ggccgcgatt aaattccaac atggatgctg atttatatgg gtataaatgg gctcgcgata
4141 atgtcgggca atcaggtgcg acaatctatc gattgtatgg gaagcccgat gcgccagagt
4201 tgtttctgaa acatggcaaa ggtagcgttg ccaatgatgt tacagatgag atggtcagac
4261 taaactggct gacggaattt atgcctcttc cgaccatcaa gcattttatc cgtactcctg
4321 atgatgcatg gttactcacc actgcgatcc ccgggaaaac agcattccag gtattagaag
4381 aatatcctga ttcaggtgaa aatattgttg atgcgctggc agtgttcctg cgccggttgc
4441 attcgattcc tgtttgtaat tgtcctttta acagcgatcg cgtatttcgt ctcgctcagg
4501 cgcaatcacg aatgaataac ggtttggttg atgcgagtga ttttgatgac gagcgtaatg
4561 gctggcctgt tgaacaagtc tggaaagaaa tgcataaact tttgccattc tcaccggatt
4621 cagtcgtcac tcatggtgat ttctcacttg ataaccttat ttttgacgag gggaaattaa
4681 taggttgtat tgatgttgga cgagtcggaa tcgcagaccg ataccaggat cttgccatcc
4741 tatggaactg cctcggtgag ttttctcctt cattacagaa acggcttttt caaaaatatg
4801 gtattgataa tcctgatatg aataaattgc agtttcattt gatgctcgat gagtttttct
4861 aagaattaat tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
4921 gttccgcgca catttccccg aaaagtgcca cctgaaattg taaacgttaa tattttgtta
4981 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
5041 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
5101 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
5161 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
5221 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
5281 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
5341 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
5401 cagggcgcgt cccattcgcc a
//
pET30b载体图谱质粒图谱pdf版和相关资料下载
pET30b载体质粒应用举例
- 上一篇：pET-30a(+)
- 下一篇：pET-30c(+)
