| 质粒名称: | pLOVE-Sox2 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 15953 |
| 存储实验室: | Miguel Ramalho-Santos |
|
载体骨架基本信息 |
|
| 载体名称: | pLOVE |
| 载体出品公司: | Clontech |
| 空载体大小: | 8263bp |
| 完整质粒大小: | 8944bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | SRY (sex determining region Y)-box 2 / Sox2 |
| 物种来源: | M. musculus (mouse) |
| 插入大小: | 2457bp |
| 标签/融合蛋白: | V5 (C terminal on insert) |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | n/a |
| 3’克隆位点: | V5 |
|
文献引用方法 |
|
| 材料和方法部分: |
pLOVE-Sox2 was a gift from Miguel Ramalho-Santos (Addgene plasmid # 15953 ; http://n2t.net/addgene:15953 ; RRID:Addgene_15953)
|
| 参考文献部分: | Generation of Induced Pluripotent Stem Cells in the Absence of Drug Selection. Blelloch R, Venere M, Yen J, Ramalho-Santos M. Cell Stem Cell. 2007 Sep 13. ():. 10.1016/j.stem.2007.08.008 PubMed 18371358 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLOVE-Sox2 8944 bp ds-DNA circular SYN 22-FEB-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pLOVE-Sox2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8944)
AUTHORS Blelloch R, Venere M, Yen J, Ramalho-Santos M
TITLE Generation of Induced Pluripotent Stem Cells in the Absence of Drug
Selection.
JOURNAL Cell Stem Cell. 2007 Sep 13. ():.
PUBMED 18371358
REFERENCE 2 (bases 1 to 8944)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 22, 2023 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8944
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 1..304
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 305..508
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
protein_bind 510..528
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 531..549
/gene="tetO"
/label=tet operator
/bound_moiety="tetracycline repressor TetR"
/note="bacterial operator O2 for the tetR and tetA genes"
protein_bind 590..614
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
protein_bind complement(1624..1648)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1701..1742
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
protein_bind 1780..1813
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
misc_feature 1869..2457
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(1922..1942)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(2340..2351)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(2460..2476)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2461..2477)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 2986..3166
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
rep_origin complement(3228..3816)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(3308..3327)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3987..4847)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4610..4629
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4848..4952)
/gene="bla"
/label=AmpR promoter
primer_bind complement(5027..5046)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 5218..5597
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 5599..5797
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
LTR 5815..5995
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 6042..6167
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6664..6897
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8420..8464
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8762..8879
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
ORIGIN
1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
481 acggtgggag gtctatataa gcagagctct ccctatcagt gatagagatc tccctatcag
541 tgatagagat cgtcgactag tccagtgtgg tggaattctg cagatatcaa caagtttgta
601 caaaaaagca ggctttaaag gaaccaattc agtcgactgg atccggtacc atgtataaca
661 tgatggagac ggagctgaag ccgccgggcc cgcagcaagc ttcggggggc ggcggcggag
721 gaggcaacgc cacggcggcg gcgaccggcg gcaaccagaa gaacagcccg gaccgcgtca
781 agaggcccat gaacgccttc atggtatggt cccgggggca gcggcgtaag atggcccagg
841 agaaccccaa gatgcacaac tcggagatca gcaagcgcct gggcgcggag tggaaacttt
901 tgtccgagac cgagaagcgg ccgttcatcg acgaggccaa gcggctgcgc gctctgcaca
961 tgaaggagca cccggattat aaataccggc cgcggcggaa aaccaagacg ctcatgaaga
1021 aggataagta cacgcttccc ggaggcttgc tggcccccgg cgggaacagc atggcgagcg
1081 gggttggggt gggcgccggc ctgggtggcg ggctgaacca gcgcatggac agctacgcgc
1141 acatgaacgg ctggagcaac ggcagctaca gcatgatgca ggagcagctg ggctacccgc
1201 agcacccggg cctcaacgct cacggcgcgg cacagatgca accgatgcac cgctacgacg
1261 tcagcgccct gcagtacaac tccatgacca gctcgcagac ctacatgaac ggctcgccca
1321 cctacagcat gtcctactcg cagcagggca cccccggtat ggcgctgggc tccatgggct
1381 ctgtggtcaa gtccgaggcc agctccagcc cccccgtggt tacctcttcc tcccactcca
1441 gggcgccctg ccaggccggg gacctccggg acatgatcag catgtacctc cccggcgccg
1501 aggtgccgga gcccgctgcg cccagtagac tgcacatggc ccagcactac cagagcggcc
1561 cggtgcccgg cacggccaaa tacggcacac tgcccctgtc gcacatgtgc tcgagatatc
1621 tagacccagc tttcttgtac aaagtggttg atatccagca cagtggcggc cgctcgagtc
1681 tagagggccc gcggttcgaa ggtaagccta tccctaaccc tctcctcggt ctcgattcta
1741 cgcgtaccgg ttagtaatga caattcgtcg agggacctaa taacttcgta tagcatacat
1801 tatacgaagt tatacatgtt taagggttcc ggttccacta ggtacaattc gatatcaagc
1861 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
1921 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
1981 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
2041 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
2101 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
2161 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
2221 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
2281 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
2341 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
2401 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
2461 gataccgtcg acctcgatcg agacctagaa aaacatggag caatcacaag tagcaataca
2521 gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga ggtgggtttt
2581 ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
2641 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
2701 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca gaactacaca
2761 ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct agtaccagtt
2821 gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt acaccctgtg
2881 agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt tgacagccgc
2941 ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc tctctggtta
3001 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
3061 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
3121 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagcat gtgagcaaaa
3181 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
3241 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
3301 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
3361 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
3421 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
3481 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
3541 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
3601 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
3661 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
3721 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
3781 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
3841 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
3901 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
3961 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
4021 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
4081 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
4141 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
4201 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
4261 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
4321 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
4381 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
4441 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
4501 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
4561 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
4621 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
4681 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
4741 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
4801 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
4861 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
4921 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
4981 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
5041 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
5101 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
5161 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
5221 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
5281 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
5341 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
5401 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
5461 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
5521 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
5581 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
5641 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
5701 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
5761 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
5821 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
5881 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
5941 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
6001 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
6061 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
6121 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
6181 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
6241 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
6301 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
6361 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
6421 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
6481 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
6541 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
6601 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
6661 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
6721 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
6781 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
6841 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
6901 gatttggggt tgctctggaa aactcatttg caccactgct gtgccctgat gaatccccta
6961 atgattttta tcaaaatcat taagttaagg tagatacaca tcttgtcata tgatcaaatg
7021 gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga actcgatatt ttacacgact
7081 ctctttacca attctgcccc gaattacact taaaacgact caacagctta acgttggctt
7141 gccacgcctt acttgactgt aaaactctca ctcttaccga acttggccgt aacctgccaa
7201 ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg attgttaggt aatcgtcacc
7261 tccacaaaga gcgactcgct gtataccgtt ggcatgctag ctttatctgt tcgggcaata
7321 cgatgcccat tgtacttgtt gactggtctg atatccgtga gcaaaaacgg cttatggtat
7381 tgcgagcttc agtcgcacta cacggtcgtt ctgttactct ttatgagaaa gcgttcccgc
7441 tttcagagca atgttcaaag aaagctcatg accaatttct agccgacctt gcgagcattc
7501 taccgagtaa caccacaccg ctcattgtca gtgatgctgg ctttaaagtg ccatggtata
7561 aatccgttga gaagctgggt tggtactggt taagtcgagt aagaggaaaa gtacaatatg
7621 cagacctagg agcggaaaac tggaaaccta tcagcaactt acatgatatg tcatctagtc
7681 actcaaagac tttaggctat aagaggctga ctaaaagcaa tccaatctca tgccaaattc
7741 tattgtataa atctcgctct aaaggccgaa aaaatcagcg ctcgacacgg actcattgtc
7801 accacccgtc acctaaaatc tactcagcgt cggcaaagga gccatggatt ctagcaacta
7861 acttacctgt tgaaattcga acacccaaac aacttgttaa tatctattcg aagcgaatgc
7921 agattgaaga aaccttccga gacttgaaaa gtcctgccta cggactaggc ctacgccata
7981 gccgaacgag cagctcagag cgttttgata tcatgctgct aatcgccctg atgcttcaac
8041 taacatgttg gcttgcgggc gttcatgctc agaaacaagg ttgggacaag cacttccagg
8101 ctaacacagt cagaaatcga aacgtactct caacagttcg cttaggcatg gaagttttgc
8161 ggcattctgg ctacacaata acaagggaag acttactcgt ggctgcaacc ctactagctc
8221 aaaatttatt cacacatggt tacgctttgg ggaaattatg aggggatctc tcagtgctgt
8281 gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg
8341 gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga
8401 atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag
8461 tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat
8521 agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt
8581 taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga
8641 caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt
8701 agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca
8761 attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa
8821 tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc
8881 gggtttatta cagggacagc agagatccag tttggttagt accgggcccg ctctagcagt
8941 tccg
//
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