| 质粒名称: | pNO183 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 101068 |
| 存储实验室: | Jeffrey Tabor |
|
载体骨架基本信息 |
|
| 载体名称: | pProTet.E333 |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | 5684 bp |
| 载体修饰: | -- |
| 载体类型: | 细菌表达，合成生物学 |
| 筛选标记: | -- |
| 载体抗性: | 氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | ppsR2 |
| 物种来源: | Rhodopseudomonas palustris CGA009 |
| 插入大小: | 1395 bp |
| 基因突变: | -- |
| GenBank编号 | WP_011157097.1 |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pNO183 was a gift from Jeffrey Tabor (Addgene plasmid # 101068 ; http://n2t.net/addgene:101068 ; RRID:Addgene_101068) |
| 参考文献部分: | Engineering an E. coli near-infrared light sensor. Ong NTX, Olson EJ, Tabor JJ. ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289. 10.1021/acssynbio.7b00289 PubMed 29091422 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pNO183 5684 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION ppsR2-bphP1 bicistronic operon plasmid.
ACCESSION .
VERSION .
KEYWORDS pNO183
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5684)
AUTHORS Ong NTX, Olson EJ, Tabor JJ
TITLE Engineering an E. coli near-infrared light sensor.
JOURNAL ACS Synth Biol. 2017 Nov 1. doi: 10.1021/acssynbio.7b00289.
PUBMED 29091422
REFERENCE 2 (bases 1 to 5684)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..5684
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 181..275
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(296..955)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind 870..889
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
primer_bind 1124..1142
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
terminator 4873..4944
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 4960..4987
/label=T7Te terminator
/note="phage T7 early transcription terminator"
rep_origin complement(5189..93)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5269..5288)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag
61 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg
121 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgac tagtgcttgg
181 attctcacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
241 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
301 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
361 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
421 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
481 ttaaatcaaa actggtgaaa ctcacccagg gattggctga gacgaaaaac atattctcaa
541 taaacccttt agggaaatag gccaggtttt caccataaca cgccacatct tgcgaatata
601 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
661 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
721 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
781 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
841 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
901 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
961 cttccttagc tcctgaaaat ctcgataact caaaaaatac gaccggtagt gatcttattt
1021 cattatggtg aaagttggaa cctcttacgg gccggccaac gtctcatttt cgccagatat
1081 cgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc gtatcacgag
1141 gccctttcgt cttcacctcg agaaaaagag tattgactta aagtctaacc tataggtata
1201 atgtgtggac atcgcgtccc aatagctcta gagaaggtta ccatggcgtc aaagtccgtt
1261 catgccgaca tcacccttct gctcgatatg gagggtgtga ttcgcgaagc caccctgtct
1321 ccgacgatgg cggccgagag cgtggacggt tggctggggc gtcgctggag cgacatcgcc
1381 ggcgccgaag gcggcgacaa ggttcgccgc atggtcgaag acgcccgccg cagcggcatc
1441 tcggctttcc gccagatcaa tcagcctttc ccgagcggcg tcgaaatccc gatcgaattc
1501 accacgatgc tgctgggcga tcgcaccggc atgatcgcgg tcggcaagaa catgcaggcg
1561 gtcaccgagc tgcattcccg gctgatcgct gcgcagcagg cgatggagcg cgactattgg
1621 cggttgcgtg aattggagac tcgctaccgc ctggtgttcg acgctgccgc cgatgcggtg
1681 atgatcgtct ccgccggcga catgcgcatc gtcgaagcca accgggcggc ggtgaatgcg
1741 atcagccgcg tcgagcgcgg caatgacgac cttgcggggc gtgatttcct cgccgaagtg
1801 gcggctgccg atcgcgatgc ggtgcgcgac atgctggccc aggtgcgtca gcgcggcacc
1861 gcactcagcg tcctcgttca tctcggccgt tacgaccgcg cctggatgct gcgcggttcg
1921 ctgatgtcgt ccgagcgtcg tcaggttttc ctgctgcact tcaccccggt gaccacgact
1981 cccgcgatcg acgacgtcga cgatgatgcc gtgctgcgcg ggctgatcga tcgcattccc
2041 gacgggttcg tcgcactgga ttcggaaggc gtcgttcgtc acgccaacca ggcgtttctc
2101 gatctggtcc agatcggctc caagcctgcg gcggtcggac gatcgctggg cgtctggatg
2161 ggtcgtccgg gcgccgatct gtccagcttg ctgacgctgc tgcggcgcta caagacggtg
2221 cggctgttcc aaacgacgat ccgcggcgag ctcggcaccg agactgaagt cgaggtgtcg
2281 gccgtcgacg gcgaggacga ccaatacatc ggcgttctga tgcgcaatgt cgcgcgacgc
2341 ctcgacgctg cggacgacca cgatgccttg cgtcaggcgc tcggcccgat cagcaagcag
2401 ctcgggcgat cctcgctgcg caagctggtg aagaacgccg tgagcattgt cgagcagcac
2461 tacgtgaagg aagcgctgtt gcgatccaag ggcaatcgca cggcaactgc cgaactgctc
2521 ggattgagcc ggcagagcct ttatgcaaaa ctcaactgct acggcttcga cgacaaaggt
2581 gtcgttgctt ctgctgccga cggtgcagag ggcgcctcag acgacgcaga ggattgagac
2641 accatctaca aaatagatag aggataatat ggcaggtcat gcctctggca gccccgcatt
2701 cgggaccgcc gatctttcga attgcgaacg tgaagagatc cacctcgccg gctcgatcca
2761 gccgcatggc gcgcttctgg tcgtcagcga gccggatcat cgcatcatcc aggccagcgc
2821 caacgccgcg gaatttctga atctcggaag cgtgctcggc gttccgctcg ccgagatcga
2881 cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag gcatgccggt
2941 cgcggtgcgc tgccggatcg gcaatccctc cacggagtac gacggtctga tgcatcggcc
3001 tccggaaggc gggctgatca tcgagctcga acgtgccggc ccgccgatcg atctgtccgg
3061 cacgctggcg ccggcgctgg agcggatccg cacggcgggc tcgctgcgcg cgctgtgcga
3121 tgacaccgcg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg tgtatcgctt
3181 cgacgagcag ggccacggcg aagtgttctc cgagcgccac gtgcccgggc tcgaatccta
3241 tttcggcaac cgctatccgt cgtcggacat tccgcagatg gcgcggcggc tgtacgagcg
3301 gcagcgcgtc cgcgtgctgg tcgacgtcag ctatcagccg gtgccgctgg agccgcggct
3361 gtcgccgctg accgggcgcg atctcgacat gtcgggctgc ttcctgcgct cgatgtcgcc
3421 gatccatctg cagtacctga agaacatggg cgtgcgcgcc accctggtgg tgtcgctggt
3481 ggtcggcggc aagctgtggg gcctggttgc ctgtcaccat tatctgccgc gcttcatcca
3541 tttcgagctg cgggcgatct gcgaactgct cgccgaagcg atcgcgacgc ggatcaccgc
3601 gcttgagagc ttcgcgcaga gccagtcgga gctgttcgtg cagcggctcg aacagcgcat
3661 gatcgaagcg atcacccgtg aaggcgattg gcgcgcagcg attttcgaca ccagccaatc
3721 gatcctgcag ccgctgcacg ccgacggttg cgcgctggtg tacgaagacc agatcaggac
3781 catcggtgac gtaccttcca cgcaggatgt tcgcgagatc gccgggtggc tcgatcgcca
3841 gccacgtgcg gcggtgacct cgaccgcgtc gctcggactc gacgtgccgg agctcgcgca
3901 tctgacgcgg atggcgagcg gcgtggtcgc ggcgccgatt tcggatcatc gcggcgagtt
3961 tctgatgtgg ttccgccccg agcgcgtcca caccgttacc tggggcggcg atccgaagaa
4021 gccgttcacg atgggcgata caccggcgga tctgtcgccg cggcgctcct tcgccaaatg
4081 gcatcaggtt gtcgaaggca cgtccgatcc gtggacggcc gccgatctcg ccgcggctcg
4141 caccatcggt cagaccgtcg ccgacatcgt gctgcaattc cgcgcggtgc ggacactgat
4201 cgcccgcgaa cagtacgaac agttttcgtc ccaggtgcac gcttcgatgc agccggtgct
4261 gatcaccgac gccgaaggcc gcatcctgct gatgaacgac tcgttccgcg acatgttgcc
4321 ggcggggtcg ccatccgccg tccatctcga cgatctcgcc gggttcttcg tcgaatcgaa
4381 cgatttcctg cgcaacgtcg ccgaactgat cgatcacggc cgcgggtggc gcggcgaagt
4441 tctgctgcgc ggcgcaggta atcgcccgtt gccgctggca gtgcgcgccg atccggtgac
4501 gcgcacggag gaccagtcgc tcggcttcgt gctgatcttc agcgacgcta ccgatcgtcg
4561 caccgcagat gccgcacgca cgcgtttcca ggaaggcatt cttgccagcg cacgtcccgg
4621 cgtgcggctc gactccaagt ccgacctctt gcacgagaag ctgctgtccg cgctggtcga
4681 gaacgcgcag cttgccgcat tggaaattac ttacggcgtc gaaaccggac gcatcgccga
4741 gctgctcgaa ggcgttcgcc agtcgatgct gcgcaccgcc gaagtgctcg gccatctggt
4801 gcagcacgcg gcgcgcacgg ccggcagcga cagctcgagc aatggctcgc agaacaagaa
4861 gtagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggactt tcgttttatc
4921 tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc gggtgggcct
4981 ttctgcgttt ataggtaccc gtggatcctc tagagtcgac ctgcaggcat gcaagcttcc
5041 taggcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
5101 cagaatcagg ggataacgca tgcaagaaca tgtgagcaaa aggccagcaa aaggccagga
5161 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
5221 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
5281 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
5341 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
5401 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
5461 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
5521 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
5581 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
5641 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agtt
//
