| 质粒名称: | pHR_SFFV |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 79121 |
| 存储实验室: | Wendell Lim |
|
载体骨架基本信息 |
|
| 载体名称: | pHR |
| 载体出品公司: | -- |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 慢病毒载体 |
| 筛选标记: | -- |
| 载体抗性: | 氨苄霉素100 μg/mL |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pHR_SFFV was a gift from Wendell Lim (Addgene plasmid # 79121 ; http://n2t.net/addgene:79121 ; RRID:Addgene_79121) |
| 参考文献部分: | Engineering Customized Cell Sensing and Response Behaviors Using Synthetic Notch Receptors. Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim WA. Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012. Epub 2016 Jan 28. 10.1016/j.cell.2016.01.012 PubMed 26830878 |
买家导航
pHRSFFV载体基本信息
pHRSFFV载体图谱质粒图谱和多克隆位点信息
pHRSFFV载体简介
pHRSFFV载体序列
LOCUS pHR_SFFV 9033 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral vector for constitutive expression of genes (SFFV
promoter).
ACCESSION .
VERSION .
KEYWORDS pHR_SFFV
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9033)
AUTHORS Morsut L, Roybal KT, Xiong X, Gordley RM, Coyle SM, Thomson M, Lim
WA
TITLE Engineering Customized Cell Sensing and Response Behaviors Using
Synthetic Notch Receptors.
JOURNAL Cell. 2016 Feb 11;164(4):780-91. doi: 10.1016/j.cell.2016.01.012.
Epub 2016 Jan 28.
PUBMED 26830878
REFERENCE 2 (bases 1 to 9033)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9033
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(1..17)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2..18)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 108..341
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter complement(366..384)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(367..384)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(674..693)
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
primer_bind complement(907..926)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1026..1048
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1086..1104)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1172..1276
/gene="bla"
/label=AmpR promoter
CDS 1277..2137
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1495..1514)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2308..2896
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2797..2816
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3050..3067
/label=L4440
/note="L4440 vector, forward primer"
primer_bind complement(3137..3157)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 3142..3471
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3322..3457
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 3384..3403
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
intron 4605..4670
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
CDS 4800..4820
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
polyA_signal 5245..5379
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(5282..5301)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 5336..5355
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 5547..6180
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 6227..6352
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 6849..7082
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 7267..7311
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 7596..7713
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
promoter 7866..8273
/label=SFFV promoter
/note="spleen focus-forming virus long terminal repeat
(LTR) promoter"
misc_feature 8443..9031
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(8496..8516)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(8914..8925)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
ORIGIN
1 cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac caatgactta
61 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
121 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
181 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
241 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
301 atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga attaattccg
361 tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat gtattaattg
421 tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg cttactgaag
481 cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg acgaaccttc
541 tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat cagcagggtc
601 atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg cgccacaggt
661 gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc tcgccacttc
721 gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc cgggggactg
781 ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa cggcctcaac
841 ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg aatggtgcac
901 tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc cgccaacacc
961 cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac
1021 cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgagacg
1081 aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
1141 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
1201 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
1261 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
1321 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
1381 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
1441 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
1501 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
1561 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
1621 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
1681 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
1741 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
1801 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
1861 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
1921 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
1981 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
2041 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
2101 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
2161 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
2221 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
2281 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
2341 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
2401 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
2461 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
2521 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
2581 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
2641 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
2701 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
2761 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
2821 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
2881 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
2941 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
3001 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
3061 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
3121 tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca
3181 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc
3241 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata
3301 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
3361 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
3421 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagcttgga
3481 cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt cagggagagg
3541 cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta
3601 taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg
3661 gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg
3721 taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg
3781 tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca tgtcgatacc
3841 gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct gaaacgcgca
3901 gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg tggtactgcg
3961 gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc aaaaccggct
4021 ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg gattgaacag
4081 ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc ttttcaacgc
4141 ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt ttccagtaag
4201 tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt tcaaaccccg
4261 ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg cacaaccgcc
4321 tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga ttaaccgtct
4381 gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg cacgtattgt
4441 gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg gctaccgtgg
4501 cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac ttctgtggtg
4561 tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat ataaaatttt
4621 taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag attccaacct
4681 atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac ctgttttgct
4741 cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat tctactcctc
4801 caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg ctaagttttt
4861 tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac accacaaagg
4921 aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc tttataagta
4981 ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg catagagtgt
5041 ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt tgtaaagggg
5101 ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag ccataccaca
5161 tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat
5221 aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa
5281 agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt
5341 ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac tcaagctaac
5401 caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta cctgtggttt
5461 catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa ttgtatttgt
5521 taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa gaagacaaga
5581 tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc agaactacac
5641 accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc tagtaccagt
5701 tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt tacaccctgt
5761 gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt ttgacagccg
5821 cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga actgctgata
5881 tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg gcctgggcgg
5941 gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt gcctgtactg
6001 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
6061 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
6121 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
6181 gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag
6241 gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc
6301 aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa
6361 gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa
6421 atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc
6481 tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct
6541 tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt
6601 gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca
6661 aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag acctggagga
6721 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa
6781 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca
6841 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca
6901 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag
6961 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc
7021 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc
7081 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct
7141 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac
7201 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag
7261 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg
7321 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg
7381 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat
7441 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga
7501 atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct
7561 cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
7621 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
7681 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat
7741 ccagtttgga tcgataagct tgatatcgaa ttcctgcagc cccgataaaa taaaagattt
7801 tatttagtct ccagaaaaag gggggaatga aagaccccac ctgtaggttt ggcaagctag
7861 ctgcagtaac gccattttgc aaggcatgga aaaataccaa accaagaata gagaagttca
7921 gatcaagggc gggtacatga aaatagctaa cgttgggcca aacaggatat ctgcggtgag
7981 cagtttcggc cccggcccgg ggccaagaac agatggtcac cgcagtttcg gccccggccc
8041 gaggccaaga acagatggtc cccagatatg gcccaaccct cagcagtttc ttaagaccca
8101 tcagatgttt ccaggctccc ccaaggacct gaaatgaccc tgcgccttat ttgaattaac
8161 caatcagcct gcttctcgct tctgttcgcg cgcttctgct tcccgagctc tataaaagag
8221 ctcacaaccc ctcactcggc gcgccagtcc tccgacagac tgagtcgccc gggggggatc
8281 tggagctctc gagaattctc acgcgtcaag tggagcaagg caggtggaca gtggatcctt
8341 gacttgcggc cgcaactccc acctgcaaca tgcgtgactg actgaggccg cgactctaga
8401 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
8461 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
8521 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
8581 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
8641 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
8701 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
8761 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
8821 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
8881 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
8941 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
9001 agtcggatct ccctttgggc cgcctccccg cat
//
