| 质粒名称: | G1333 DddAtox-N–dSpCas9 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 157833 |
| 存储实验室: | David Liu |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 完整质粒大小: | 8409 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Mach1 |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | bpNLS–G1333 DddAtox-N–dSpCas9–UGI–UGI–bpNLS |
|
文献引用方法 |
|
| 材料和方法部分: |
G1333 DddAtox-N–dSpCas9 was a gift from David Liu (Addgene plasmid # 157833 ; http://n2t.net/addgene:157833 ; RRID:Addgene_157833)
|
| 参考文献部分: | A bacterial cytidine deaminase toxin enables CRISPR-free mitochondrial base editing. Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F, Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR. Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi: 10.1038/s41586-020-2477-4. 10.1038/s41586-020-2477-4 PubMed 32641830 |
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载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS G1333 DddAtox-N–dSpCas9 8409 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION expresses split DddAtox-Cas9 construct in mammalian cells.
ACCESSION .
VERSION .
KEYWORDS G1333 DddAtox-N–dSpCas9
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8409)
AUTHORS Mok BY, de Moraes MH, Zeng J, Bosch DE, Kotrys AV, Raguram A, Hsu F,
Radey MC, Peterson SB, Mootha VK, Mougous JD, Liu DR
TITLE A bacterial cytidine deaminase toxin enables CRISPR-free
mitochondrial base editing.
JOURNAL Nature. 2020 Jul 8. pii: 10.1038/s41586-020-2477-4. doi:
10.1038/s41586-020-2477-4.
PUBMED 32641830
REFERENCE 2 (bases 1 to 8409)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8409
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 132..335
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 286..306
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 377..396
/label=T7
/note="T7 promoter, forward primer"
promoter 377..395
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 445..465
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 694..4794
/codon_start=1
/product="catalytically dead mutant of the Cas9
endonuclease from the Streptococcus pyogenes Type II
CRISPR/Cas system"
/label=dCas9
/note="RNA-guided DNA-binding protein that lacks
endonuclease activity due to the D10A mutation in the RuvC
catalytic domain and the H840A mutation in the HNH
catalytic domain"
/translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4825..5073
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5104..5352
/codon_start=1
/product="uracil-DNA glycosylase inhibitor from a Bacillus
subtilis bacteriophage (Mol et al., 1995)"
/label=UGI
/translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHTA
YDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
CDS 5395..5415
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 5424..5441
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
primer_bind complement(5464..5481)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5470..5694
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(5765..5781)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5765..5781)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(5778..5800)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 5789..5805
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(5813..5843)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5858..5879
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(5996..6013)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(6167..6755)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(6247..6266)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(6926..7786)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 7549..7568
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(7787..7891)
/gene="bla"
/label=AmpR promoter
primer_bind complement(7970..7989)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 8161..131
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
ORIGIN
1 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
61 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
121 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
181 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
241 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
301 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagatccgct
361 agagatccgc ggccgctaat acgactcact atagggagag ccgccaccat gaaacggaca
421 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtcggcag ctacgccctg
481 ggtccgtatc agattagcgc cccgcagctg ccagcttaca atggtcagac cgtgggtacc
541 ttctactatg tgaacgacgc gggcggtctg gagagcaagg tgtttagcag cggcggttct
601 ggaggatcta gcggaggatc ctctggcagc gagacaccag gaacaagcga gtcagcaaca
661 ccagagagca gtggcggcag cagcggcggc agcgacaaga agtacagcat cggcctggcc
721 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
781 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
841 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
901 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
961 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
1021 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
1081 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
1141 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
1201 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
1261 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
1321 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
1381 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
1441 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
1501 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
1561 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
1621 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
1681 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
1741 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
1801 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
1861 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
1921 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
1981 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
2041 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
2101 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
2161 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
2221 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
2281 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
2341 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
2401 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
2461 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
2521 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
2581 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
2641 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
2701 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
2761 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
2821 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
2881 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
2941 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
3001 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
3061 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
3121 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
3181 aaccggctgt ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac
3241 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
3301 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
3361 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
3421 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
3481 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
3541 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
3601 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
3661 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
3721 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
3781 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
3841 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
3901 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
3961 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
4021 ctgcccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
4081 tacggcggct tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
4141 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
4201 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
4261 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
4321 aagagaatgc tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc
4381 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
4441 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
4501 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
4561 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
4621 cacctgttta ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc
4681 atcgaccgga agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag
4741 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgacagcggc
4801 gggagcggcg ggagcggggg gagcactaat ctgagcgaca tcattgagaa ggagactggg
4861 aaacagctgg tcattcagga gtccatcctg atgctgcctg aggaggtgga ggaagtgatc
4921 ggcaacaagc cagagtctga catcctggtg cacaccgcct acgacgagtc cacagatgag
4981 aatgtgatgc tgctgacctc tgacgccccc gagtataagc cttgggccct ggtcatccag
5041 gattctaacg gcgagaataa gatcaagatg ctgagcggag gatccggagg atctggaggc
5101 agcaccaacc tgtctgacat catcgagaag gagacaggca agcagctggt catccaggag
5161 agcatcctga tgctgcccga agaagtcgaa gaagtgatcg gaaacaagcc tgagagcgat
5221 atcctggtcc ataccgccta cgacgagagt accgacgaaa atgtgatgct gctgacatcc
5281 gacgccccag agtataagcc ctgggctctg gtcatccagg attccaacgg agagaacaaa
5341 atcaaaatgc tgtctggcgg ctcaaaaaga accgccgacg gcagcgaatt cgagcccaag
5401 aagaagagga aagtctaacc ggtcatcatc accatcacca ttgagtttaa acccgctgat
5461 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
5521 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
5581 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
5641 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
5701 aggcggaaag aaccagctgg ggctcgatac cgtcgacctc tagctagagc ttggcgtaat
5761 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
5821 gagccggaag cataaagtgt aaagcctagg gtgcctaatg agtgagctaa ctcacattaa
5881 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
5941 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
6001 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
6061 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
6121 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
6181 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
6241 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
6301 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
6361 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
6421 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
6481 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
6541 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
6601 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
6661 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
6721 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
6781 ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
6841 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
6901 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
6961 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
7021 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
7081 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
7141 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
7201 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
7261 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
7321 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
7381 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
7441 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
7501 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
7561 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
7621 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
7681 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
7741 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
7801 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
7861 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
7921 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
7981 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
8041 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
8101 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
8161 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
8221 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
8281 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
8341 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
8401 aagtgtatc
//
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