| 出品公司: | -- |
|---|---|
| 载体名称: | pPZP102 |
| 质粒类型: | 植物载体；农杆菌双元表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | lac |
| 载体大小: | 7028 bp |
| 5' 测序引物及序列: | M13 Reverse：CAGGAAACAGCTATGAC |
| 3' 测序引物及序列: | M13/pUC Forward：CCCAGTCACGACGTTGTAAAACG |
| 载体标签: | 无 |
| 载体抗性: | chloramphenicol |
| 筛选标记: | 无 |
| 克隆菌株: | HB101 |
| 宿主细胞（系）: | 植物细胞、农杆菌 |
| 备注: | 重组质粒可用蓝白斑法筛选 |
| 产品目录号: | -- |
| 稳定性: | 稳表达 |
| 组成型/诱导型: | 诱导型 |
| 病毒/非病毒: | 非病毒 |
LOCUS Exported 7028 bp ds-DNA circular SYN 27-JUL-2016
DEFINITION Agrobacterium binary vector for plant transformation, with a
chloramphenicol-resistance gene. The MCS is reversed in pPZP101.
ACCESSION U10457
VERSION .
KEYWORDS pPZP102
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7028)
AUTHORS Hajdukiewicz P, Svab Z, Maliga P.
TITLE The small, versatile pPZP family of Agrobacterium binary vectors for
plant transformation.
JOURNAL Plant Mol. Biol. 1994;25:989-94.
PUBMED 7919218
REFERENCE 2 (bases 1 to 7028)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, July 27, 2016
COMMENT The GenBank sequence was corrected by inserting a G at position
2249.
FEATURES Location/Qualifiers
source 1..7028
/organism="synthetic DNA construct"
/lab_host="Plant Cells"
/mol_type="other DNA"
CDS 1187..1816
/codon_start=1
/product="stability protein from Pseudomonas plasmid pVS1"
/note="pVS1 StaA"
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2245..3318
/codon_start=1
/product="replication protein from Pseudomonas plasmid
pVS1"
/note="pVS1 RepA"
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3384..3578
/note="pVS1 oriV"
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
misc_feature 3922..4062
/note="bom"
/note="basis of mobility region from pBR322"
rep_origin complement(4248..4836)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5107..5766)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/note="CmR"
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
misc_feature 6294..6318
/note="LB T-DNA repeat"
/note="left border repeat from nopaline C58 T-DNA"
CDS complement(6390..6623)
/codon_start=1
/gene="lacZ (truncated)"
/product="LacZ-alpha fragment of beta-galactosidase"
/note="lacZ-alpha"
/translation="MTMITNSSSVPGDPLESTCRHASLALAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEC"
primer_bind 6533..6549
/note="M13 fwd"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 6553..6609
/note="MCS"
/note="pUC18 multiple cloning site"
primer_bind complement(6619..6635)
/note="M13 rev"
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 6621..6623
/note="ATG"
protein_bind 6643..6659
/bound_moiety="lac repressor encoded by lacI"
/note="lac operator"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(6667..6697)
/note="lac promoter"
/note="promoter for the E. coli lac operon"
misc_feature 6891..6915
/note="RB T-DNA repeat"
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
2221 aacccccaag cccgaggaat cggcgtgagc ggtcgcaaac catccggccc ggtacaaatc
2281 ggcgcggcgc tgggtgatga cctggtggag aagttgaagg ccgcgcaggc cgcccagcgg
2341 caacgcatcg aggcagaagc acgccccggt gaatcgtggc aagcggccgc tgatcgaatc
2401 cgcaaagaat cccggcaacc gccggcagcc ggtgcgccgt cgattaggaa gccgcccaag
2461 ggcgacgagc aaccagattt tttcgttccg atgctctatg acgtgggcac ccgcgatagt
2521 cgcagcatca tggacgtggc cgttttccgt ctgtcgaagc gtgaccgacg agctggcgag
2581 gtgatccgct acgagcttcc agacgggcac gtagaggttt ccgcagggcc ggccggcatg
2641 gccagtgtgt gggattacga cctggtactg atggcggttt cccatctaac cgaatccatg
2701 aaccgatacc gggaagggaa gggagacaag cccggccgcg tgttccgtcc acacgttgcg
2761 gacgtactca agttctgccg gcgagccgat ggcggaaagc agaaagacga cctggtagaa
2821 acctgcattc ggttaaacac cacgcacgtt gccatgcagc gtacgaagaa ggccaagaac
2881 ggccgcctgg tgacggtatc cgagggtgaa gccttgatta gccgctacaa gatcgtaaag
2941 agcgaaaccg ggcggccgga gtacatcgag atcgagctag ctgattggat gtaccgcgag
3001 atcacagaag gcaagaaccc ggacgtgctg acggttcacc ccgattactt tttgatcgat
3061 cccggcatcg gccgttttct ctaccgcctg gcacgccgcg ccgcaggcaa ggcagaagcc
3121 agatggttgt tcaagacgat ctacgaacgc agtggcagcg ccggagagtt caagaagttc
3181 tgtttcaccg tgcgcaagct gatcgggtca aatgacctgc cggagtacga tttgaaggag
3241 gaggcggggc aggctggccc gatcctagtc atgcgctacc gcaacctgat cgagggcgaa
3301 gcatccgccg gttcctaatg tacggagcag atgctagggc aaattgccct agcaggggaa
3361 aaaggtcgaa aaggtctctt tcctgtggat agcacgtaca ttgggaaccc aaagccgtac
3421 attgggaacc ggaacccgta cattgggaac ccaaagccgt acattgggaa ccggtcacac
3481 atgtaagtga ctgatataaa agagaaaaaa ggcgattttt ccgcctaaaa ctctttaaaa
3541 cttattaaaa ctcttaaaac ccgcctggcc tgtgcataac tgtctggcca gcgcacagcc
3601 gaagagctgc aaaaagcgcc tacccttcgg tcgctgcgct ccctacgccc cgccgcttcg
3661 cgtcggccta tcgcggccgc tggccgctca aaaatggctg gcctacggcc aggcaatcta
3721 ccagggcgcg gacaagccgc gccgtcgcca ctcgaccgcc ggcgcccaca tcaaggcacc
3781 ctgcctcgcg cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac
3841 ggtcacagct tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc
3901 gggtgttggc gggtgtcggg gcgcagccat gacccagtca cgtagcgata gcggagtgta
3961 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
4021 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg
4081 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
4141 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
4201 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
4261 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
4321 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
4381 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
4441 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
4501 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
4561 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
4621 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
4681 actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
4741 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
4801 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
4861 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gcatgatata
4921 tctcccaatt tgtgtagggc ttattatgca cgcttaaaaa taataaaagc agacttgacc
4981 tgatagtttg gctgtgagca attatgtgct tagtgcatct aatcgcttga gttaacgccg
5041 gcgaagcggc gtcggcttga acgaatttct agctagagga tcgcaccaat aactgcctta
5101 aaaaaattac gccccgccct gccactcatc gcagtactgt tgtaattcat taagcattct
5161 gccgacatgg aagccatcac aaacggcatg atgaacctga atcgccagcg gcatcagcac
5221 cttgtcgcct tgcgtataat atttgcccat tgtgaaaacg ggggcgaaga agttgtccat
5281 attggccacg tttaaatcaa aactggtgaa actcacccag ggattggctg agacgaaaaa
5341 catattctca ataaaccctt tagggaaata ggccaggttt tcaccgtaac acgccacatc
5401 ttgcgaatat atgtgtagaa actgccggaa atcgtcgtgg tattcactcc agagcgatga
5461 aaacgtttca gtttgctcat ggaaaacggt gtaacaaggg tgaacactat cccatatcac
5521 cagctcaccg tctttcattg ccatacggaa ctccggatga gcattcatca ggcgggcaag
5581 aatgtgaata aaggccggat aaaacttgtg cttatttttc tttacggtct ttaaaaaggc
5641 cgtaatatcc agctgaacgg tctggttata ggtacattga gcaactgact gaaatgcctc
5701 aaaatgttct ttacgatgcc attgggatat atcaacggtg gtatatccag tgattttttt
5761 ctccatgatg tttaactttg ttttagggcg actgccctgc tgcgtaacat cgttgctgct
5821 ccataacatc aaacatcgac ccacggcgta acgcgcttgc tgcttggatg cccgaggcat
5881 agactgtacc ccaaaaaaac atgtcataac aagaagccat gaaaaccgcc actgcgccgt
5941 taccaccgct gcgttcggtc aaggttctgg accagttgcg tgacggcagt tacgctactt
6001 gcattacagc ttacgaaccg aacgaggctt atgtccactg ggttcgtgcc cgaattgatc
6061 acaggcagca acgctctgtc atcgttacaa tcaacatgct accctccgcg agatcatccg
6121 tgtttcaaac ccggcagctt agttgccgtt cttccgaata gcatcggtaa catgagcaaa
6181 gtctgccgcc ttacaacggc tctcccgctg acgccgtccc ggactgatgg gctgcctgta
6241 tcgagtggtg attttgtgcc gagctgccgg tcggggagct gttggctggc tggtggcagg
6301 atatattgtg gtgtaaacaa attgacgctt agacaactta ataacacatt gcggacgttt
6361 ttaatgtact gaattaacgc cgaattgctc tagcattcgc cattcaggct gcgcaactgt
6421 tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa agggggatgt
6481 gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg
6541 acggccagtg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc gggtaccgag
6601 ctcgaattcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
6661 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
6721 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
6781 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttggagcttg
6841 agcttggatc agattgtcgt ttcccgcctt cagtttaaac tatcagtgtt tgacaggata
6901 tattggcggg taaacctaag agaaaagagc gtttattaga ataacggata tttaaaaggg
6961 cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag ggttcccctc
7021 gggatcaa
//
