| 质粒名称: | pCT-tRNA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 133813 |
| 存储实验室: | Geraldine Butler |
|
载体骨架基本信息 |
|
| 载体名称: | pAYCU268 |
| 载体出品公司: | Clontech |
| 完整质粒大小: | 10610bp |
| 载体类型: | CRISPR |
| 筛选标记: | SAT1 (resistance to Nourseothricin) |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | cassette for the expression of the sgRNA from the Ashbya gossypii RNA pol II TEF1 |
| 物种来源: | Synthetic |
| 启动子: | gRNA/shRNA sequenceUnknown |
| 克隆方法: | Gibson Cloning |
| 5’克隆位点: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pCT-tRNA was a gift from Geraldine Butler (Addgene plasmid # 133813 ; http://n2t.net/addgene:133813 ; RRID:Addgene_133813) |
| 参考文献部分: | Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species. Lombardi L, Oliveira-Pacheco J, Butler G. mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi: 10.1128/mSphere.00125-19. 10.1128/mSphere.00125-19 PubMed 30867327 |
买家导航
pCT-tRNA载体质粒基本信息
pCT-tRNA质粒图谱载体图谱和pCT-tRNA载体序列质粒序列多克隆位点信息
pCT-tRNA质粒载体简介
pCT-tRNA质粒序列载体序列
LOCUS pCT-tRNA 10610 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CRISPR-Cas9 system for genetic manipulation of Candida tropicalis.
ACCESSION .
VERSION .
KEYWORDS pCT-tRNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10610)
AUTHORS Lombardi L, Oliveira-Pacheco J, Butler G
TITLE Plasmid-Based CRISPR-Cas9 Gene Editing in Multiple Candida Species.
JOURNAL mSphere. 2019 Mar 13;4(2). pii: 4/2/e00125-19. doi:
10.1128/mSphere.00125-19.
PUBMED 30867327
REFERENCE 2 (bases 1 to 10610)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10610
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..344
/label=TEF promoter
/note="Ashbya gossypii TEF promoter"
misc_RNA 458..533
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(629..647)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
terminator 659..848
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind 995..1017
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 1009..1026
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 1010..1026
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 1033..1052
/label=T7
/note="T7 promoter, forward primer"
promoter 1033..1051
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 3194..7297
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 7310..7330
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7334..7354
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7358..7378
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 7379..7444
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
promoter complement(7679..7697)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
primer_bind complement(7680..7697)
/label=SP6
/note="SP6 promoter, forward primer"
primer_bind complement(7715..7731)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7715..7731)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7728..7750)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7739..7755
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7763..7793)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7808..7829
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(8767..9355)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8847..8866)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(9526..10386)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 10149..10168
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(10387..10491)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gacatggagg cccagaatac cctccttgac agtcttgacg tgcgcagctc aggggcatga
61 tgtgactgtc gcccgtacat ttagcccata catccccatg tataatcatt tgcatccata
121 cattttgatg gccgcacggc gcgaagcaaa aattacggct cctcgctgcg gacctgcgag
181 cagggaaacg ctcccctcac agacgcgttg aattgtcccc acgccgcgcc cctgtagaga
241 aatataaaag gttaggattt gccactgagg ttcttctttc atatacttcc ttttaaaatc
301 ttgctaggat acagttctca catcacatcc gaacataaac aaccaaacaa agagcttaaa
361 atgggcgtgt ggcgtagttg gtagcgcgtt cccttagcat gggaaaggtc atgagttcga
421 ttcttatctc gtccaggaag agctcgcgag ctcttccgtt ttagagctag aaatagcaag
481 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttggc
541 cggcatggtc ccagcctcct cgctggcgcc ggctgggcaa catgcttcgg catggcgaat
601 gggactctcg agtcatgtaa ttagttatgt cacgcttaca ttcacgccct ccccccacat
661 ccgctctaac cgaaaaggaa ggagttagac aacctgaagt ctaggtccct atttattttt
721 ttatagttat gttagtatta agaacgttat ttatatttca aatttttctt ttttttctgt
781 acagacgcgt gtacgcatgt aacattatac tgaaaacctt gcttgagaag gttttgggac
841 gctcgaaggc tttaacgagc tcactagttc cattcgccat tcaggctgcg caactgttgg
901 gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct
961 gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg
1021 gccagtgaat tgtaatacga ctcactatag ggcgaattgg gcccgtccaa ctggtggatc
1081 agtatcggtt atgtcatcta aaaacaaaat tgatgctgca aaacgatgga atatcccagt
1141 tgtctcggtt gcgtatttgt gggaaatttt agaactctcg actaacaaat cgcatcttat
1201 catgcccgat attacagatt tacagtggtg tatttttgca cctagcaact ataataaacc
1261 taaatcatta ttggagtatg taaagaattt ggataaagcc agtagagaaa gttcctttgg
1321 tcccaagagt caagaaattg atgcacaaga acaacctaca atggataatc tggtgaagtt
1381 gccctcacca cgaagagtta acctgaaaca aaaatatggt aaattggtac gaggtaaatc
1441 tccgaaatca attaaacgga aattactaga agctgcaaat tcgtttactg atggtcaaaa
1501 tgatcacgat attaatccag atgtaacact tgaagaggac atgatgtcac aaataagata
1561 tcaagacaat gagtctatga tcaaccaaga aagattatta gagaaattgg atggatcagc
1621 tttaccactg gagaccctga tacagcatac ctgtaaacgg agtagaaaaa aataactgta
1681 taatatttgc tattactata aaacttgtat cattattctt atatggggtt gtagccctaa
1741 ttactcttac tttctcgtga tttgcacgtg tttgtttttg tatttgtgcg actaatatta
1801 tagcgcattt gttatcttag tgaaatcagt catacctttt gttctccccc taactatcta
1861 ctattctcat gaaactgcaa gaaaaaagtt ctgctgttca ctttctctcg ttcaactttt
1921 acaaagtaaa aaaaaaaaaa aaaatttttt ttttcctcgt catcttgatt ttacttcttc
1981 ttctttttcc tttttccttt cttcacactt tatagtcata atcaatcatc gattgctaga
2041 attaccggat ctatgaaaat ttcggtgatc cctgagcagg tggcggaaac attggatgct
2101 gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc tatccgacca aggctttgaa
2161 ctatctacca gaagtgtgag cccctaccgg aaggattaca tctcggatga tgactctgat
2221 gaagactctg cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa gattgaactc
2281 aactcaacat ggaacgatct agcctctatc gaacacattg ttgtgtcgca cacgcaccga
2341 ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact aagcagacag
2401 ctccttggca tacgattaga gacacaaacg aacaatgtac ctgcctgcaa tttgtacgca
2461 aaatgtggct ttactctcgg cggcattgac ctcttcacgt ataaaactag acctcaagtc
2521 tcgaacgaaa cagcgatgta ctggtactgg ttctcgggag cacaggatga cgccatgcag
2581 ggcgcctaag aatatacgtc gttattttgt gttatacact cagctacaat ttcgatttcc
2641 cgctcatgat gaggccccat gcttcctctg tcaactcctc gtacttgtca ccattcaacc
2701 agtagatcac atcggtgccg ctttctccct tgggaagttt ttggttctta aattggttct
2761 tgggcacctt gtggttatgt gagcggccgc ggtaccgacg tcacccgctc tgacggttac
2821 ccaatgcggt tataagccaa cagtctgttg tgcgactagg ctcgcttggc acctgcacag
2881 atgctgcgac agctctcacg cacagaaatg gtcacctaga gtcgatttcc gcgcctcgtt
2941 gccgccggtc tccgcgcggt gaatcctgta catagtcatc tccgattcac tttcactaga
3001 cgaatccggc acatgagtga tccggcgtgc acacaatagc aatctccctg cacacaccgg
3061 gacgcgattg ccgggtaatc cctggttggg tcgtttctgc ctcgttgttt gataccagcg
3121 ctcacccctt tcgaaaaatt tacttttgac taggtattaa tatagtatag caaactgcag
3181 ctcgagggat ccgatggata aaaagtatag tattggttta gatattggta ctaactctgt
3241 gggttgggca gttatcactg acgaatataa agttccatca aagaaattta aggtgttagg
3301 taacactgac agacactcaa taaaaaagaa tcttatcggt gctcttttgt tcgactccgg
3361 tgaaactgcc gaggctacac gtttaaaaag aacagcaaga agaagatata cccgtagaaa
3421 aaatagaata tgttatttac aagaaatctt ttctaatgaa atggctaaag ttgatgattc
3481 ctttttccat agattggaag agtcattttt ggttgaagaa gacaaaaagc atgagagaca
3541 tccaatcttt gggaatatag ttgatgaagt ggcttaccat gaaaaatatc ctaccattta
3601 tcatttaaga aagaaattgg tagattcaac tgataaagct gaccttagat taatctattt
3661 agcacttgcc catatgatta aatttagagg tcattttttg attgaaggtg atttgaaccc
3721 agataattct gacgtggata aattatttat tcaattagtc caaacctaca accaattatt
3781 tgaggaaaat ccaattaatg ctagtggtgt cgatgccaaa gctatattat cagccagatt
3841 atcaaaatct agacgtttgg aaaatttgat tgcccaattg ccaggagaaa aaaagaatgg
3901 attatttgga aacttgatcg cattatcatt gggtttgaca ccaaatttta aatctaattt
3961 tgatttagct gaagatgcta aattacaatt atcaaaagac acctatgacg acgatttgga
4021 caatttactt gctcaaattg gtgatcaata tgcagatttg ttcttagctg ctaaaaactt
4081 atctgatgct attttgttgt ctgatatttt gagagtgaac acagaaataa ccaaagctcc
4141 attatcagca tctatgatca aacgttatga tgaacaccat caggatttga ctttattgaa
4201 agctttggtg agacaacaat tgccagagaa gtataaagaa atctttttcg atcaatctaa
4261 aaacgggtat gcaggttata ttgatggggg tgcctcccaa gaggaatttt acaaatttat
4321 aaaacctatt ttagaaaaga tggatgggac tgaggaactt ttggtcaaat tgaacagaga
4381 agatttgtta cgtaaacaga gaacttttga taatggtagt atacctcacc aaattcattt
4441 gggtgagttg catgcaattt taagaagaca agaagatttt tatccatttt taaaagataa
4501 tagagaaaaa atcgagaaaa ttttaacctt tagaattcca tactatgttg ggcctttggc
4561 tagaggtaat tcaagatttg cctggatgac acgtaaatca gaagaaacta ttaccccttg
4621 gaattttgaa gaggttgttg ataaaggagc atcagcacag agttttattg aaagaatgac
4681 caatttcgat aaaaacttac caaatgaaaa agttttacca aaacattcct tgttatacga
4741 atattttact gtttacaatg aacttacaaa ggttaaatat gttactgaag gtatgcgtaa
4801 gccagccttt ttatctggag aacagaaaaa ggcaatagtt gatttattgt ttaaaacaaa
4861 tagaaaagtt actgttaaac aattaaaaga agattacttt aagaaaattg aatgttttga
4921 ttcagttgaa atcagtggtg ttgaagacag atttaatgct agtttaggaa cttaccatga
4981 tttacttaaa attatcaaag ataaagattt cttggataac gaagaaaatg aagacatttt
5041 agaagacatt gttttaacct taactttatt cgaagataga gagatgattg aagaacgttt
5101 gaagacttat gcacatttgt ttgacgataa agtgatgaaa cagttgaaaa gaagacgtta
5161 tactggatgg ggtagattgt ctcgtaaatt gatcaatgga attagagata aacaaagtgg
5221 taaaactatc ttggactttt tgaaatctga cggatttgct aatagaaatt tcatgcaatt
5281 gatccacgac gatagtttga catttaaaga agacatccaa aaggcccaag tgagtgggca
5341 aggtgattca ttacatgaac atattgcaaa tttagccgga tctcctgcta ttaagaaagg
5401 gatattacaa actgttaaag ttgtggatga attagtgaaa gtaatgggaa gacataaacc
5461 tgaaaacatt gtcattgaga tggcaagaga aaatcaaact acacaaaaag gacagaaaaa
5521 tagtagagaa cgtatgaaaa gaatagaaga gggtattaaa gaattgggta gtcaaatatt
5581 gaaagaacac ccagtggaaa atacccagtt gcaaaatgaa aaattatatc tttactacct
5641 tcaaaatgga cgtgatatgt atgttgatca ggaattagat ataaatagac tttcagatta
5701 tgatgtagat catatagttc cacaatcttt cttgaaagat gattccatag acaataaagt
5761 attaactaga agtgataaaa atagaggtaa aagtgataat gtcccaagtg aggaagtcgt
5821 caaaaagatg aaaaattact ggcgtcaact tttgaatgct aaattaatta ctcaaagaaa
5881 atttgataat ttgactaaag cagaaagagg tgggctttct gaattagata aagccgggtt
5941 cattaaaaga caattggtcg aaactagaca aattactaaa catgttgccc aaattttaga
6001 ttcccgtatg aacactaagt atgacgaaaa tgataagtta atacgtgagg ttaaagtcat
6061 tactttaaaa tcaaaacttg tctctgattt cagaaaggat ttccaattct ataaagttag
6121 agaaattaat aattatcatc atgctcatga tgcatatttg aatgctgtag ttggaactgc
6181 tttaatcaag aaatacccta aattagaatc tgaatttgta tatggtgatt acaaagtcta
6241 tgatgttaga aagatgattg ctaaatcaga acaagaaatt ggtaaagcta cagctaaata
6301 cttcttttac tctaacatta tgaatttctt taaaacagaa attactttgg caaacggtga
6361 aattagaaaa agacctctta ttgaaacaaa tggtgagact ggagagatag tttgggacaa
6421 agggcgtgat ttcgctactg ttagaaaagt tttatcaatg ccacaagtta acattgtaaa
6481 gaaaacagag gttcaaactg gtggtttctc aaaagaaagt attttgccta aaagaaatag
6541 tgataaattg attgccagaa aaaaggattg ggatccaaag aaatatggtg gtttcgactc
6601 accaaccgta gcctattctg ttttggttgt ggcaaaggtt gaaaagggta aaagtaaaaa
6661 gcttaaatca gtaaaagaac ttttgggtat tacaataatg gaaagaagtt cctttgaaaa
6721 gaaccctatt gattttttgg aagctaaagg ttataaggaa gtaaagaagg acttaataat
6781 caaattgcct aaatattctt tatttgaatt agaaaatggg agaaaaagaa tgttggcttc
6841 tgctggagaa ttgcaaaagg gtaatgaatt agcattgcct tccaaatatg ttaacttctt
6901 gtatttagct tcacactatg aaaagttgaa agggtcacca gaagataacg agcaaaaaca
6961 attatttgtt gaacaacaca aacactactt agatgagatt atagaacaaa ttagtgaatt
7021 cagtaaaaga gtgatattag ctgatgcaaa tttagataaa gttttgtcag cctataacaa
7081 acatagagat aagccaatta gagaacaagc agaaaacatt attcacttat ttacccttac
7141 caatttagga gcacctgctg ctttcaagta ttttgataca acaattgatc gtaaaagata
7201 tacctcaaca aaagaagtct tagacgccac cttaattcat caatcaatca ctggattgta
7261 tgagacaaga attgatttgt ctcaattggg tggtgatgaa ggggctgatc ctaagaagaa
7321 aagaaaagtt gatccaaaga aaaagcgtaa ggtggatcct aagaaaaaga gaaaggttga
7381 ctacaaagac catgacggtg attataaaga tcatgacatc gactacaagg atgacgatga
7441 caagtgatat ctcctaggta acaaagcata caagtgtaag aatttcaatt ttatcagtaa
7501 cttattttac cttataatat atgtttatta ctcctacttc tctagtcttc ttggtcccaa
7561 tttccaggtc tacgttgctg aggaacttgg ggaccaccag cacgcttggc catgatgatt
7621 tggtcgatgg acaagatggt ggtagcggct tcagtagcca aatgcatagc ttgagtattc
7681 tatagtgtca cctaaatagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt
7741 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
7801 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt
7861 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt
7921 tgcgtattga aaaatccggg gtagcgatga ggtagtgcaa gttataccag agagcctcaa
7981 tttgaacgtg gtactgcaca caaaccgtag ccctaaccct aattaaatgc acgtgaccca
8041 cacaattttc aaccaccaac aacacaccaa aaatgtatgt acactcggag tggaaaatat
8101 ttccccaggc ataattgtgg ttgccaatta tagtggagtg tttgttatta gtataggagt
8161 actcctttta tgtggaaaaa tcgaaaaaca taaaacagtg cagctataca ctaacaaatg
8221 gtatacccat ggtcagacaa caccaaacaa cacacccgta atggttggtt tggctaaata
8281 gagcatctca ttttgtgtat tattttatgt tttggatatt tttagcttga aatttatata
8341 aaaatatttt actccaattt tcttgccaaa ttttgtacaa aaagtaaaaa atagaacttc
8401 caatttttgt taaacaaact tcaatctaac aaccgggata gtgtttgggg ggctgtgtaa
8461 caccactggt gtaataaaag tgccaatatt tggaaattaa ttttttggtg gtggacgttt
8521 cttcctaaaa cacctagatg tgctagtata attgcacaac cagcacgcac actgcgtctg
8581 gccggtcctg gactacattt tgtctctaac attcgtagct gcagttcggc tgcggcgagc
8641 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
8701 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
8761 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
8821 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
8881 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
8941 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
9001 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
9061 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
9121 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
9181 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
9241 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
9301 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
9361 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
9421 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
9481 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
9541 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
9601 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
9661 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
9721 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
9781 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
9841 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
9901 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
9961 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
10021 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
10081 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
10141 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
10201 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
10261 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
10321 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
10381 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
10441 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
10501 ccgaaaagtg ccacctgatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
10561 gcatcaggaa ccggtagctt gcctcgtccc cgccgggtca cccggccagc
//
