pDG1730

LOCUS       pDG1730                7602 bp DNA     circular SYN 17-JUL-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2024 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(5773..6361)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6532..7392)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7393..7497)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aacaaaattc tccagtcttc acatcggttt gaaaggagga agcggaagaa tgaagtaaga
       61 gggatttttg actccgaagt aagtcttcaa aaaatcaaat aaggagtgtc aagaatgttt
      121 gcaaaacgat tcaaaacctc tttactgccg ttattcgctg gatttttatt gctgtttcat
      181 ttggttctgg caggaccggc ggctgcgagt gctgaaacgg cgaacaaatc gaatgagctt
      241 acagcaccgt cgatcaaaag cggaaccatt cttcatgcat ggaattggtc gttcaatacg
      301 ttaaaacaca atatgaagga tattcatgat gcaggatata cagccattca gacatctccg
      361 attaaccaag taaaggaagg gaatcaagga gataaaagca tgtcgaactg gtactggctg
      421 tatcagccga catcgtatca aattggcaac cgttacttag gtactgaaca agaatttaaa
      481 gaaatgtgtg cagccgctga agaatatggc ataaaggtca ttgttgacgc ggtcatcaat
      541 cataccacca gtgattatgc cgcgatttcc aatgaggtta agagtattcc aaactggaca
      601 catggaaaca cacaaattaa aaactggtct gatcggatcc tagaagctta tcgaattcct
      661 gcagccctgg cgaatggcga ttttcgttcg tgaatacatg ttataataac tataactaat
      721 aacgtaacgt gactggcaag agatattttt aaaacaatga ataggtttac acttacttta
      781 gttttatgga aatgaaagat catatcatat ataatctaga ataaaattaa ctaaaataat
      841 tattatctag ataaaaaatt tagaagccaa tgaaatctat aaataaacta aattaagttt
      901 atttaattaa caactatgga tataaaatag gtactaatca aaatagtgag gaggatatat
      961 ttgaatacat acgaacaaat taataaagtg aaaaaaatac ttcggaaaca tttaaaaaat
     1021 aaccttattg gtacttacat gtttggatca ggagttgaga gtggactaaa accaaatagt
     1081 gatcttgact ttttagtcgt cgtatctgaa ccattgacag atcaaagtaa agaaatactt
     1141 atacaaaaaa ttagacctat ttcaaaaaaa ataggagata aaagcaactt acgatatatt
     1201 gaattaacaa ttattattca gcaagaaatg gtaccgtgga atcatcctcc caaacaagaa
     1261 tttatttatg gagaatggtt acaagagctt tatgaacaag gatacattcc tcagaaggaa
     1321 ttaaattcag atttaaccat aatgctttac caagcaaaac gaaaaaataa aagaatatac
     1381 ggaaattatg acttagagga attactacct gatattccat tttctgatgt gagaagagcc
     1441 attatggatt cgtcagagga attaatagat aattatcagg atgatgaaac caactctata
     1501 ttaactttat gccgtatgat tttaactatg gacacgggta aaatcatacc aaaagatatt
     1561 gcgggaaatg cagtggctga atcttctcca ttagaacata gggagagaat tttgttagca
     1621 gttcgtagtt atcttggaga gaatattgaa tggactaatg aaaatgtaaa tttaactata
     1681 aactatttaa ataacagatt aaaaaaatta taaaaaaatt gaaaaaatgg tggaaacact
     1741 tttttcaatt tttttgtttt attatttaat atttgggaaa tattcattct aattggtaat
     1801 cagattttag aaaacaataa acccttgcat agggggatct cgacatggat gagcgatgat
     1861 gatatccgtt taggctgggc ggtgatagct tctcgttcag gcagtacgcc tcttttcttt
     1921 tccagacctg agggaggcgg aaatggtgtg aggttcccgg ggaaaagcca aataggcgat
     1981 cgcgggagtg ctttatttga agatcaggct atcactgcgg tcaatagatt tcacaatgtg
     2041 atggctggac agcctgagga actctcgaac ccgaatggaa acaaccagat atttatgaat
     2101 cagcgcggct cacatggcgt tgtgctggca aatgcaggtt catcctctgt ctctatcaat
     2161 acggcaacaa aattgcctga tggcaggtat gacaataaag ctggagcggg ttcatttcaa
     2221 gtgaacgatg gtaaactgac aggcacgatc aatgccaggt ctgtagctgt gctttatcct
     2281 gatgatattg caaaagcgcc tcatgttttc cttgagaatt acaaaacagg tgtaacacat
     2341 tctttcaatg atcaactgac gattaccttg cgtgcagatg cgaatacaac aaaagccgtt
     2401 tatcaaatca ataatggacc agacgacagg cgtttaagga tggagatcaa ttcacaatcg
     2461 gaaaaggaga tccaatttgg caaaacatac accatcatgt taaaaggaac gaacagtgat
     2521 ggtgtaacga ggaccgagaa atacagtttt gttaaaagag atccagcgtc ggccaaaacc
     2581 atcggctatc aaaatccgaa tcattggagc caggtaaatg cttatatcta taaacatgat
     2641 gggagccgag taattgaatt gaccggatct tggcctggaa aaccaatgac taaaaatgca
     2701 gacggaattt acacgctgac gctgcctgcg gacacggata caaccaacgc aaaagtgatt
     2761 tttaataatg gcagcgccca agtgcccggt cagaatcagc ctggctttga ttacgtgcta
     2821 aatggtttat ataatgactc gggcttaagc ggttctcttc cccattgagg gcaaggctag
     2881 acgggactta ccgaaagaaa ccatcaatga tggtttcttt tttgttcata aatcagacaa
     2941 aacttttctc ttgcaaaagt ttgtgaagtg ttgcacaata taaatgtgaa atacttcaca
     3001 aacaaaaaga catcaaagag aaacataccc tgcaaggatg ctgatattgt ctgcatttgc
     3061 gccggagcaa accaaaaacc tggtgagaca cgccttgaat tagtagaaaa gaacttgaag
     3121 attttcaaag gcatcgttag tgaagtcatg gcgagcggat ttgacggcat tttcttagtc
     3181 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
     3241 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
     3301 caaggatcgc tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg
     3361 gcgatttatg ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc
     3421 ctataccttg tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctactga
     3481 agtggatttc tttaagagct cctttaactt cctcaccagt agttgtatcg gtaccataag
     3541 tagaagcagc aacccaagta gctttaccag catccggttc aaccagcata gtaagaatct
     3601 tactggacat cggcagttct tcgaacagtg cgccaactac cagctctttc tgcagttcat
     3661 tcagggcacc ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca
     3721 tcctgtctct tgatccatgg attacgcgtt aacccgggcc cgcggatgca tatgatcaga
     3781 tcctttaact ctggcaaccc tcaaaattga atgagacatg ctacacctcc ggataataaa
     3841 tatatataaa cgtatataga tttcataaag tctaacacac tagacttatt tacttcgtaa
     3901 ttaagtcgtt aaaccgtgtg ctctacgacc aaaactataa aacctttaag aactttcttt
     3961 ttttacaaga aaaaagaaat tagataaatc tctcatatct tttattcaat aatcgcatcc
     4021 gattgcagta taaatttaac gatcactcat catgttcata tttatcagag ctcgtgctat
     4081 aattatacta attttataag gaggaaaaaa tatgggcatt tttagtattt ttgtaatcag
     4141 cacagttcat tatcaaccaa acaaaaaata agtggttata atgaatcgtt aataagcaaa
     4201 attcatataa ccaaattaaa gagggttata atgaacgaga aaaatataaa acacagtcaa
     4261 aactttatta cttcaaaaca taatatagat aaaataatga caaatataag attaaatgaa
     4321 catgataata tctttgaaat cggctcagga aaaggccatt ttacccttga attagtaaag
     4381 aggtgtaatt tcgtaactgc cattgaaata gaccataaat tatgcaaaac tacagaaaat
     4441 aaacttgttg atcacgataa tttccaagtt ttaaacaagg atatattgca gtttaaattt
     4501 cctaaaaacc aatcctataa aatatatggt aatatacctt ataacataag tacggatata
     4561 atacgcaaaa ttgtttttga tagtatagct aatgagattt atttaatcgt ggaatacggg
     4621 tttgctaaaa gattattaaa tacaaaacgc tcattggcat tacttttaat ggcagaagtt
     4681 gatatttcta tattaagtat ggttccaaga gaatattttc atcctaaacc taaagtgaat
     4741 agctcactta tcagattaag tagaaaaaaa tcaagaatat cacacaaaga taaacaaaag
     4801 tataattatt tcgttatgaa atgggttaac aaagaataca agaaaatatt tacaaaaaat
     4861 caatttaaca attccttaaa acatgcagga attgacgatt taaacaatat tagctttgaa
     4921 caattcttat ctcttttcaa tagctataaa ttatttaata agtaagttaa gggatgcata
     4981 aactgcatcc cttaacttgt ttttcgtgtg cctatttttt gtgaatcgat tatgtctttt
     5041 gcgcagtcgg cttaaaccag ttttcgctgg tgcgaaaaaa gagtgtcttg tgacacctaa
     5101 attcaaaatc tatcggtcag atttataccg atttgatttt atatattctt gaataacata
     5161 cgccgagtta tcacataaaa gcgggaacca atcatcaaat ttaaacttca ttgcataatc
     5221 cattaaactc ttaaattcta cgattccttg ttcatcaata aactcaatca tttctttaat
     5281 taatttatat ctatctgttg ttgttttctt taataattca tcaacatcta caccgccata
     5341 aactatcata tcttcttttt gatatttaaa tttattagga tcttaaggcc taggtctaga
     5401 gtctttgttt tgacgccatt agcgtacgta acaatcctcg ttaaaggaca aggacctgag
     5461 cggaagtgta tcgtacagta gacggagtat actagtatag tctatagtcc gtggaattat
     5521 tatatttatc tccgacgata ttctcatcag tgaaatccag ctggagttct ttagcaaatt
     5581 tttttattag ctgaacttag tattagtggc catactcctc caatccaaag ctatttagaa
     5641 agattactat atcctcaaac aggcggtaac cggcctcttc atcgggaatg cgcgcgacct
     5701 tcagcatcgc cggcatgtcc ccctggcgga cgggaagtat ccagctcgag gtcgggccgc
     5761 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     5821 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     5881 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     5941 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     6001 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     6061 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     6121 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     6181 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct
     6241 gaagccagtt accttcggaa aaagagttga tagctcttga tccggcaaac aaaccaccgc
     6301 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     6361 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     6421 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     6481 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
     6541 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
     6601 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
     6661 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
     6721 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
     6781 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
     6841 cattgctgca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
     6901 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
     6961 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
     7021 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
     7081 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
     7141 ggcgtcaaca cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
     7201 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
     7261 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
     7321 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
     7381 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
     7441 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
     7501 atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta
     7561 taaaaatagg cgtatcacga ggccctttcg tcttcaagaa tt
//