| 出品公司: | Agilent |
|---|---|
| 载体名称: | pBAV1K-T5-sfGFP |
| 质粒类型: | ORF |
| 高拷贝/低拷贝: | 低拷贝 |
| 载体大小: | 3.6kb |
| 载体抗性: | Kan |
| 克隆菌株: | DH5 alpha; 等 |
| 宿主细胞（系）: | 大肠杆菌 |
| 产品目录号: | #50054 |
| 启动子: | T5 |
| 温度: | 37度 |
| 质粒用途: | 蛋白表达 |
买家导航
pBAV1K-T5-sfGFP载体质粒基本信息
pBAV1K-T5-sfGFP质粒图谱载体图谱和pBAV1K-T5-sfGFP载体序列质粒序列多克隆位点信息
pBAV1K-T5-sfGFP质粒载体简介
pBAV1K-T5-sfGFP质粒序列载体序列
LOCUS pBAV1K-T5-sfGFP 3626 bp DNA circular SYN 15-JUL-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3626)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 15, 2024 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..3626
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 26..70
/label=T5 promoter
/note="bacteriophage T5 promoter for E. coli RNA
polymerase, with embedded lac operator"
protein_bind 46..62
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 77..88
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 95..808
/codon_start=1
/product="GFP variant that folds robustly even when fused
to poorly folded proteins (Pedelacq et al., 2006)"
/label=superfolder GFP
/translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
FVTAAGITHGMDELYK"
misc_feature 815..835
/label=BioBrick suffix
/note="universal suffix for all parts"
terminator 886..972
/gene="Escherichia coli rrnB"
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
CDS 2303..2314
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
terminator complement(2516..2610)
/label=lambda t0 terminator
/note="transcription terminator from phage lambda"
CDS complement(2626..3420)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEEYRCVKDTEGMSPAKVYKLVGENEILYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEGYED
EQSPEKTIELYAECIRLFHSIDISDCPYTNSLDGRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature join(3626,1..21)
/label=BioBrick prefix
/note="BioBrick prefix for parts that do not start with
""ATG"""
ORIGIN
1 aattcgcggc cgcttctaga ggaaatcata aaaaatttat ttgctttgtg agcggataac
61 aattataata ctagagaaag aggagaaata ctagatgagc aaaggagaag aacttttcac
121 tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt
181 ccgtggagag ggtgaaggtg atgctacaaa cggaaaactc acccttaaat ttatttgcac
241 tactggaaaa ctacctgttc cgtggccaac acttgtcact actctgacct atggtgttca
301 atgcttttcc cgttatccgg atcacatgaa acggcatgac tttttcaaga gtgccatgcc
361 cgaaggttat gtacaggaac gcactatatc tttcaaagat gacgggacct acaagacgcg
421 tgctgaagtc aagtttgaag gtgataccct tgttaatcgt atcgagttaa agggtattga
481 ttttaaagaa gatggaaaca ttcttggaca caaactcgag tacaacttta actcacacaa
541 tgtatacatc acggcagaca aacaaaagaa tggaatcaaa gctaacttca aaattcgcca
601 caacgttgaa gatggttccg ttcaactagc agaccattat caacaaaata ctccaattgg
661 cgatggccct gtccttttac cagacaacca ttacctgtcg acacaatctg tcctttcgaa
721 agatcccaac gaaaagcgtg accacatggt ccttcttgag tttgtaactg ctgctgggat
781 tacacatggc atggatgagc tctacaaata gtaatactag tagcggccgc tgcaggcctc
841 agggcccgat cgatgccgcc gcttaattaa ttaatccaga ggcatcaaat aaaacgaaag
901 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg
961 agtaggacaa atccgccgcc ctagacctag tgtcatttta tttcccccgt ttcagcatca
1021 agaacctttg cataacttgc tctatatcca cactgataat tgccctcaaa ccataatcta
1081 aaggcgctag agtttgttga aacaatatct tttacatcat tcgtatttaa aattccaaac
1141 tccgctcccc taaggcgaat aaaagccatt aaatcttttg tatttaccaa attatagtca
1201 tccactatat ctaagagtaa attcttcaat tctctttttt ggctttcatc aagtgttata
1261 tagcggtcaa tatcaaaatc attaatgttc aaaatatctt ttttgtcgta tatatgttta
1321 ttcttagcaa tagcgtcctt tgattcatga gtcaaatatt catatgaacc tttgatataa
1381 tcaagtatct caacatgagc aactgaacta ttccccaatt ttcgcttaat cttgttccta
1441 acgctttcta ttgttacagg atttcgtgca atatatataa cgtgatagtg tggtttttta
1501 tagtgctttc catttcgtat aacatcacta ctattccatg tatctttatc ttttttttcg
1561 tccatatcgt gtaaaggact gacagccata gatacgccca aactctctaa tttttccttc
1621 caatcattag gaattgagtc aggatataat aaaaatccaa aatttctagc tttagtattt
1681 ttaatagcca tgatataatt accttatcaa aaacaagtag cgaaaactcg tatccttcta
1741 aaaacgcgag ctttcgctta ttttttttgt tctgattcct ttcttgcata ttcttctata
1801 gctaacgccg caaccgcaga ttttgaaaaa cctttttgtt tcgccatatc tgttaatttt
1861 ttatcttgct cttttgtcag agaaatcata actctttttt tcgattctga aatcaccatt
1921 taaaaaactc caatcaaata attttataaa gttagtgtat cactttgtaa tcataaaaac
1981 aacaataaag ctacttaaat atagatttat aaaaaacgtt ggcgaaaacg ttggcgattc
2041 gttggcgatt gaaaaacccc ttaaaccctt gagccagttg ggatagagcg tttttggcac
2101 aaaaattggc actcggcact taatgggggg tcgtagtacg gaagcaaaat tcgcttcctt
2161 tccccccatt tttttccaaa ttccagattt ttttcaaaaa ttttccagcg ctaccgctcg
2221 gcaaaattgc aagcaatttt taaaatcaaa cccatgaggg aatttcattc cctcatactc
2281 ccttgagcct cctccaaccg aaatagaagg gcgctgcgct tattatttca ttcagtcatc
2341 ggctttcata atctaacaga caacatcttc gctgcaaagc cacgctacgc tcaagggctt
2401 ttacgctacg ataacgcctg ttttaacgat tatgccgata actaaacgaa ataaacgcta
2461 aaacgtctca gaaacgattt tgagacgttt taataaaaaa tcgcctagtg cttggattct
2521 caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga tggagttctg
2581 aggtcattac tggatctatc aacgggagtc caagcgagct cggtactaaa acaattcatc
2641 cagtaaaata taatatttta ttttctccca atcaggcttg atccccagta agtcaaaaaa
2701 tagctcgaca tactgttctt ccccgatatc ctccctgatc gaccggacgc agaaggcaat
2761 gtcataccac ttgtccgccc tgccgcttct cccaagatca ataaagccac ttactttgcc
2821 atctttcaca aagatgttgc tgtctcccag gtcgccgtgg gaaaagacaa gttcctcttc
2881 gggcttttcc gtctttaaaa aatcatacag ctcgcgcgga tctttaaatg gagtgtcttc
2941 ttcccagttt tcgcaatcca catcggccag atcgttattc agtaagtaat ccaattcggc
3001 taagcggccg tctaagctat tcgtataggg acaatccgat atgtcgatgg agtgaaagag
3061 cctgatgcac tccgcataca gctcgatagt cttttcaggg ctttgttcat cttcataccc
3121 ttccgagcaa aggacgccat cggcctcact catgagcaga ttgctccagc catcatgccg
3181 ttcaaagtgc aggacctttg gaacaggcag ctttccttcc agccatagca tcatgtcctt
3241 ttcccgttcc acatcatagg tggtcccttt ataccggctg tccgtcattt ttaaatatag
3301 gatttcattt tctcccacca gcttatatac cttagcagga gacattcctt ccgtatcttt
3361 tacgcagcgg tattcttcga tcagtttttt caattccggt gatattctca ttttagccat
3421 ttattatttc cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac
3481 aagacgaact ccaattcact gttccttgca ttctaaaacc ttaaatacag aaaacagcct
3541 tttcaaagtt gttttcaaag ttggcgtata acatagtatc gacggagccg attttgaaac
3601 cacaattatg atagaatttg acgtcg
//
