质粒名称: | GW1-Peredox-mCherry |
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出品公司: | 爱迪基因 |
目录编号: | 32380 |
存储实验室: | Gary Yellen |
载体骨架基本信息 |
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载体名称: | GW1 |
载体出品公司: | British Biotechnology |
载体抗性: | 氨苄霉素 |
空载体大小: | 5300 bp |
载体类型: | 哺乳细胞表达 |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | Peredox-mCherry |
插入大小: | 2800 bp |
启动子 | CMV |
克隆方法: | 限制性内切酶 |
5' 克隆位点: | PspOMI(未破坏) |
3' 克隆位点: | SalI(未破坏) |
5' 测序引物 | CMV-F |
3' 测序引物 | SV40pA-R |
文献引用方法 |
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材料和方法部分: | GW1-Peredox-mCherry was a gift from Gary Yellen (Addgene plasmid # 32380) |
参考文献部分: | Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714 |
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载体基本信息
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载体简介
载体序列
LOCUS GW1-Peredox-mCherry 8045 bp DNA SYN DEFINITION GW1-Peredox-mCherry ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8045 /organism="GW1-Peredox-mCherry" /mol_type="other DNA" CDS complement(316..1281) /label="ORF frame 3" CDS 640..3408 /label="ORF frame 1" CDS complement(1453..2004) /label="ORF frame 3" CDS complement(2202..2765) /label="ORF frame 1" CDS complement(2497..3429) /label="ORF frame 3" gene 2701..3405 /label="mCherry" /gene="mCherry" misc_feature 2878..2931 /label="Q66M (mCherry)" terminator 3533..3664 /label="SV40_PA_terminator" misc_feature 3621..3640 /label="EBV_rev_primer" promoter complement(3684..3702) /label="M13_reverse_primer" misc_feature complement(3701..3723) /label="M13_pUC_rev_primer" promoter complement(3737..3766) /label="lac_promoter" rep_origin complement(4075..4694) /label="pBR322_origin" gene complement(4849..5709) /label="Ampicillin" /gene="Ampicillin" CDS complement(4849..5709) /label="ORF frame 3" promoter complement(5788..5816) /label="AmpR_promoter" misc_feature complement(5975..5997) /label="pGEX_3_primer" misc_feature complement(6184..6203) /label="SV40pro_F_primer" misc_feature complement(6234..6377) /label="lacZ_a" CDS complement(6234..6761) /label="ORF frame 1" misc_feature 6348..6370 /label="M13_pUC_fwd_primer" promoter 6363..6379 /label="M13_forward20_primer" promoter 6929..7505 /label="CMV_immearly_promoter" misc_feature 7008..7295 /label="CAG_enhancer" misc_feature 7462..7482 /label="CMV_fwd_primer" promoter 7463..7532 /label="CMV_promoter" promoter 7475..7594 /label="CMV2_promoter" misc_feature 7506..7525 /label="pCEP_fwd_primer" misc_feature 7508..7532 /label="LNCX_primer" CDS complement(8000..413) /label="ORF frame 1" ORIGIN 1 ggacatgggc tcttctccgg tagcggcgga gcttctacat ccgagccctg ctcccatgcc 61 tccagcgact catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg 121 cacagcacga tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg 181 tctgaaaatg agctcgggga gcgggcttgc accgctgacg catttggaag acttaaggca 241 gcggcagaag aagatgcagg cagctgagtt gttgtgttct gataagagtc agaggtaact 301 cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc 361 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt 421 ttctgcagtc accgtccttg acacgaagct tcccggccac agcaatcgat ccatgacaca 481 accagtgaca agcagtcccc cagtgaaacc gcatccgcat ccgagtccga taccgataaa 541 gattctgaat cggagtgagt gccgcgtccg agagcgttcc ctgtccacgt ccaccatcgg 601 cggagcaggt gtgcctgagg cctctagatt gggcccacca tggttgctag caaagttcct 661 gaagcagcca tttccagact gattacttac ctgagaattc tggaagagct ggaagcccaa 721 ggtgtacacc gcaccgcctc cgagcaactg ggcgagctgg cccaggtcac cgccttccag 781 gttgataagg acctgtccta cttcggctcc tacggcactg acggcgttgg ctacactgta 841 ccggtcctca agagagaact gcgccatatc ctcggtctga accgcaaatg gggcctgtgc 901 atcgtgggga tgggccgcct gggatccgct cttgctgatt ggcctggttt tggcgagagc 961 ttcgagctgc gcggtttctt cgatgttgac ccaggtatgg ttggtcggcc ggttcgcggt 1021 ggtgttatcg aacacgttga tctgctgccc cagcgcgtac ctggtcgcat cgaaatcgct 1081 ctgctgacgg ttccacgcga ggcagcacag aaggctgccg acctgctggt tgccgctggc 1141 atcaagggca tcctgaactt cgctccagtt gtactggagg ttcccaagga agtggctgtt 1201 gagaacgttg acatcctggc cggtctgacg cgtctgagct ttgccattct gaaccccacg 1261 tggagcgcag caggtgggca tggtttcact gctcacaacg tgtatatcat ggctgacaaa 1321 cagaagaacg gtatcaaggc caacttcaag atccgccaca acatcgaaga cggcggcgtt 1381 cagctggctg atcactacca gcagaacacc ccaatcggcg acggccctgt tctgctgcct 1441 gacaaccact acttaagcat ccagtctaaa ctgtccaaag acccaaacga gaagcgcgac 1501 cacatggttc tgctggaatt tgttaccgcc gctggcatca cccacggcat ggacgaactg 1561 tacaagggcg gcaccggggg atctatggtt tccaaaggcg aggaactgtt cactggtgtt 1621 gttcctatcc tggttgaact ggacggcgac gttaacggcc acaaattcag cgttagcggg 1681 gaaggcgaag gagacgctac ctacggcaag ctgactctga agttcatctg caccactggc 1741 aagctgcctg ttccttggcc taccctggtt actaccttct cctatggtgt tatggttttc 1801 gccagatatc ctgaccacat gaagcagcac gacttcttca agagcgccat gcctgagggc 1861 tacgttcagg aacgcaccat ctttttcaag gacgacggca actataagac acgcgctgag 1921 gttaagttcg agggcgacac tctggttaac cgcatcgagc tgaagggcat cgacttcaag 1981 gaggacggca acatcctggg ccataagctt gaatataaca ccaaggtacc tgaagccgcc 2041 atcagccgcc tcatcactta cctccgcatc ctggaggagc tcgaagccca aggcgttcac 2101 cgcactgcaa gcgagcagct gggcgaactg gcacaggtta cagcattcca ggttgacgaa 2161 gatctgtcct atttcggttc ctacggcacc gacggtgttg gctacacagt ccctgtcctg 2221 aagcgcgagc tgcgccacat cctgggcctg aaccgcaagt ggggcttatg catcgttggc 2281 atgggccgcc tcggtagtgc actggcagac tggccgggct ttggtgaatc tttcgaactg 2341 cgcggcttct tcgacgttga tccaggcatg gttgggcgcc ctgttcgcgg tggcgttatc 2401 gagcatgttg acctcctgcc acagcgcgtc ccgggtcgca tcgagatcgc cctgctgacc 2461 gttcctcgcg aagctgcaca gaaggcagcc gacttactag tcgccgctgg tatcaaggga 2521 attctgaact tcgcccctgt tgttctggag gttcctaagg aagttgctgt tgaaaacgtt 2581 gatttcctgg ccggcctgac ccgcctgagc ttcgcaatcc tgaatcctaa gtggcgcgaa 2641 gaaatgatgg gttccggaac tgggggcaac gcctctgacg gtggtgggtc tggtggtatg 2701 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg 2761 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc 2821 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc 2881 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc 2941 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg 3001 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc 3061 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg 3121 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc 3181 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag 3241 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac 3301 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc 3361 gccgagggcc gccactccac cggcggcatg gacgaactgt acaagtgagt cgacgaattc 3421 attgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc 3481 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat 3541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt 3601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg 3661 gatccgtacc gagctcgcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 3721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta 3781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa 3841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat 3901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 4021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 4081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 4141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 4201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4261 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt 4321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4501 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 5041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 5101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 5161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 5221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5701 aatactcata ctcttccttt ttcaataagc ggccgcggcc atgccggcca ctagtctcga 5761 gttattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 5821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 5881 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct 5941 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga 6001 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag 6061 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga 6121 gagtgcacca tatgaagacg tcgcctcctc actacttctg gaatagctca gaggccgagg 6181 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg cgccattcgc cattcaggct 6241 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa 6301 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg 6361 ttgtaaaacg acggccagtg ccatctgcag tgaataataa aatgtgtgtt tgtccgaaat 6421 acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg cgatagtggt gtttatcgcc 6481 gatagagatg gcgatattgg aaaaatcgat atttgaaaat atggcatatt gaaaatgtcg 6541 ccgatgtgag tttctgtgta actgatatcg ccatttttcc aaaagttgat ttttgggcat 6601 acgcgatatc tggcgatacg cttatatcgt ttacggggga tggcgataga cgcctttggt 6661 gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg atataggtga cagacgatat 6721 gaggctatat cgccgataga ggcgacatca agctggcaca tggccaatgc atatcgatct 6781 atacattgaa tcaatattgg ccattagcca tattattcat tggttatata gcataaatca 6841 atattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg 6901 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat 6961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 7021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 7081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 7141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 7201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 7261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 7321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 7381 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 7441 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 7501 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg 7561 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa 7621 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc 7681 ccttggcttc ttatgcatgc tatactgttt ttggcttggg gtctatacac ccccgcttcc 7741 tcatgttata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac 7801 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca 7861 actctcttta ttggctatat gccaatacac tgtccttcag agactgacac ggactctgta 7921 tttttacagg atggggtctc atttattatt tacaaattca catatacaac accaccgtcc 7981 ccagtgcccg cagtttttat taaacataac gtgggatctc cacgcgaatc tcgggtacgt 8041 gttcc //
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