GW1-Peredox-mCherry

价格:1200元

联系方式:I47-825O-882O

相关技术服务:质粒构建    基因合成    质粒大提

买家导航

载体基本信息

质粒名称: GW1-Peredox-mCherry 
出品公司: 爱迪基因
目录编号: 32380
存储实验室: Gary Yellen

载体骨架基本信息
载体名称: GW1
载体出品公司: British Biotechnology
载体抗性: 氨苄霉素
空载体大小: 5300 bp
载体类型: 哺乳细胞表达
高拷贝/低拷贝: 高拷贝
生长条件: 37 ℃

插入基因信息
插入基因名称: Peredox-mCherry
插入大小: 2800 bp
启动子 CMV
克隆方法: 限制性内切酶
5' 克隆位点: PspOMI(未破坏)
3' 克隆位点: SalI(未破坏)
5' 测序引物 CMV-F
3' 测序引物 SV40pA-R

文献引用方法
材料和方法部分: GW1-Peredox-mCherry was a gift from Gary Yellen (Addgene plasmid # 32380)
参考文献部分: Imaging Cytosolic NADH-NAD(+) Redox State with a Genetically Encoded Fluorescent Biosensor. Hung YP, Albeck JG, Tantama M, Yellen G. Cell Metab. 2011 Oct 5;14(4):545-54. 10.1016/j.cmet.2011.08.012 PubMed 21982714

载体图谱质粒图谱和多克隆位点信息

GW1-Peredox-mCherry载体图谱

载体简介

载体序列

LOCUS       GW1-Peredox-mCherry	8045 bp 	DNA	SYN
DEFINITION  GW1-Peredox-mCherry
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..8045
                     /organism="GW1-Peredox-mCherry"
                     /mol_type="other DNA"
     CDS             complement(316..1281)
                     /label="ORF frame 3"
     CDS             640..3408
                     /label="ORF frame 1"
     CDS             complement(1453..2004)
                     /label="ORF frame 3"
     CDS             complement(2202..2765)
                     /label="ORF frame 1"
     CDS             complement(2497..3429)
                     /label="ORF frame 3"
     gene            2701..3405
                     /label="mCherry"
                     /gene="mCherry"
     misc_feature    2878..2931
                     /label="Q66M (mCherry)"
     terminator      3533..3664
                     /label="SV40_PA_terminator"
     misc_feature    3621..3640
                     /label="EBV_rev_primer"
     promoter        complement(3684..3702)
                     /label="M13_reverse_primer"
     misc_feature    complement(3701..3723)
                     /label="M13_pUC_rev_primer"
     promoter        complement(3737..3766)
                     /label="lac_promoter"
     rep_origin      complement(4075..4694)
                     /label="pBR322_origin"
     gene            complement(4849..5709)
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             complement(4849..5709)
                     /label="ORF frame 3"
     promoter        complement(5788..5816)
                     /label="AmpR_promoter"
     misc_feature    complement(5975..5997)
                     /label="pGEX_3_primer"
     misc_feature    complement(6184..6203)
                     /label="SV40pro_F_primer"
     misc_feature    complement(6234..6377)
                     /label="lacZ_a"
     CDS             complement(6234..6761)
                     /label="ORF frame 1"
     misc_feature    6348..6370
                     /label="M13_pUC_fwd_primer"
     promoter        6363..6379
                     /label="M13_forward20_primer"
     promoter        6929..7505
                     /label="CMV_immearly_promoter"
     misc_feature    7008..7295
                     /label="CAG_enhancer"
     misc_feature    7462..7482
                     /label="CMV_fwd_primer"
     promoter        7463..7532
                     /label="CMV_promoter"
     promoter        7475..7594
                     /label="CMV2_promoter"
     misc_feature    7506..7525
                     /label="pCEP_fwd_primer"
     misc_feature    7508..7532
                     /label="LNCX_primer"
     CDS             complement(8000..413)
                     /label="ORF frame 1"
ORIGIN
    1 ggacatgggc tcttctccgg tagcggcgga gcttctacat ccgagccctg ctcccatgcc
   61 tccagcgact catggtcgct cggcagctcc ttgctcctaa cagtggaggc cagacttagg
  121 cacagcacga tgcccaccac caccagtgtg ccgcacaagg ccgtggcggt agggtatgtg
  181 tctgaaaatg agctcgggga gcgggcttgc accgctgacg catttggaag acttaaggca
  241 gcggcagaag aagatgcagg cagctgagtt gttgtgttct gataagagtc agaggtaact
  301 cccgttgcgg tgctgttaac ggtggagggc agtgtagtct gagcagtact cgttgctgcc
  361 gcgcgcgcca ccagacataa tagctgacag actaacagac tgttcctttc catgggtctt
  421 ttctgcagtc accgtccttg acacgaagct tcccggccac agcaatcgat ccatgacaca
  481 accagtgaca agcagtcccc cagtgaaacc gcatccgcat ccgagtccga taccgataaa
  541 gattctgaat cggagtgagt gccgcgtccg agagcgttcc ctgtccacgt ccaccatcgg
  601 cggagcaggt gtgcctgagg cctctagatt gggcccacca tggttgctag caaagttcct
  661 gaagcagcca tttccagact gattacttac ctgagaattc tggaagagct ggaagcccaa
  721 ggtgtacacc gcaccgcctc cgagcaactg ggcgagctgg cccaggtcac cgccttccag
  781 gttgataagg acctgtccta cttcggctcc tacggcactg acggcgttgg ctacactgta
  841 ccggtcctca agagagaact gcgccatatc ctcggtctga accgcaaatg gggcctgtgc
  901 atcgtgggga tgggccgcct gggatccgct cttgctgatt ggcctggttt tggcgagagc
  961 ttcgagctgc gcggtttctt cgatgttgac ccaggtatgg ttggtcggcc ggttcgcggt
 1021 ggtgttatcg aacacgttga tctgctgccc cagcgcgtac ctggtcgcat cgaaatcgct
 1081 ctgctgacgg ttccacgcga ggcagcacag aaggctgccg acctgctggt tgccgctggc
 1141 atcaagggca tcctgaactt cgctccagtt gtactggagg ttcccaagga agtggctgtt
 1201 gagaacgttg acatcctggc cggtctgacg cgtctgagct ttgccattct gaaccccacg
 1261 tggagcgcag caggtgggca tggtttcact gctcacaacg tgtatatcat ggctgacaaa
 1321 cagaagaacg gtatcaaggc caacttcaag atccgccaca acatcgaaga cggcggcgtt
 1381 cagctggctg atcactacca gcagaacacc ccaatcggcg acggccctgt tctgctgcct
 1441 gacaaccact acttaagcat ccagtctaaa ctgtccaaag acccaaacga gaagcgcgac
 1501 cacatggttc tgctggaatt tgttaccgcc gctggcatca cccacggcat ggacgaactg
 1561 tacaagggcg gcaccggggg atctatggtt tccaaaggcg aggaactgtt cactggtgtt
 1621 gttcctatcc tggttgaact ggacggcgac gttaacggcc acaaattcag cgttagcggg
 1681 gaaggcgaag gagacgctac ctacggcaag ctgactctga agttcatctg caccactggc
 1741 aagctgcctg ttccttggcc taccctggtt actaccttct cctatggtgt tatggttttc
 1801 gccagatatc ctgaccacat gaagcagcac gacttcttca agagcgccat gcctgagggc
 1861 tacgttcagg aacgcaccat ctttttcaag gacgacggca actataagac acgcgctgag
 1921 gttaagttcg agggcgacac tctggttaac cgcatcgagc tgaagggcat cgacttcaag
 1981 gaggacggca acatcctggg ccataagctt gaatataaca ccaaggtacc tgaagccgcc
 2041 atcagccgcc tcatcactta cctccgcatc ctggaggagc tcgaagccca aggcgttcac
 2101 cgcactgcaa gcgagcagct gggcgaactg gcacaggtta cagcattcca ggttgacgaa
 2161 gatctgtcct atttcggttc ctacggcacc gacggtgttg gctacacagt ccctgtcctg
 2221 aagcgcgagc tgcgccacat cctgggcctg aaccgcaagt ggggcttatg catcgttggc
 2281 atgggccgcc tcggtagtgc actggcagac tggccgggct ttggtgaatc tttcgaactg
 2341 cgcggcttct tcgacgttga tccaggcatg gttgggcgcc ctgttcgcgg tggcgttatc
 2401 gagcatgttg acctcctgcc acagcgcgtc ccgggtcgca tcgagatcgc cctgctgacc
 2461 gttcctcgcg aagctgcaca gaaggcagcc gacttactag tcgccgctgg tatcaaggga
 2521 attctgaact tcgcccctgt tgttctggag gttcctaagg aagttgctgt tgaaaacgtt
 2581 gatttcctgg ccggcctgac ccgcctgagc ttcgcaatcc tgaatcctaa gtggcgcgaa
 2641 gaaatgatgg gttccggaac tgggggcaac gcctctgacg gtggtgggtc tggtggtatg
 2701 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
 2761 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
 2821 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
 2881 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
 2941 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
 3001 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
 3061 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
 3121 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
 3181 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
 3241 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
 3301 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
 3361 gccgagggcc gccactccac cggcggcatg gacgaactgt acaagtgagt cgacgaattc
 3421 attgatcata atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc
 3481 acacctcccc ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat
 3541 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
 3601 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
 3661 gatccgtacc gagctcgcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
 3721 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
 3781 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
 3841 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
 3901 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
 3961 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
 4021 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
 4081 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
 4141 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
 4201 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
 4261 ttcgggaagc gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt
 4321 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
 4381 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
 4441 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
 4501 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
 4561 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
 4621 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
 4681 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
 4741 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
 4801 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
 4861 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
 4921 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
 4981 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
 5041 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
 5101 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
 5161 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
 5221 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
 5281 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
 5341 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
 5401 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
 5461 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
 5521 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
 5581 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
 5641 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
 5701 aatactcata ctcttccttt ttcaataagc ggccgcggcc atgccggcca ctagtctcga
 5761 gttattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
 5821 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
 5881 tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
 5941 ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga
 6001 cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag
 6061 cgggtgttgg cgggtgtcgg ggctggctta actatgcggc atcagagcag attgtactga
 6121 gagtgcacca tatgaagacg tcgcctcctc actacttctg gaatagctca gaggccgagg
 6181 cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcg cgccattcgc cattcaggct
 6241 gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agctggcgaa
 6301 agggggatgt gctgcaaggc gattaagttg ggtaacgcca gggttttccc agtcacgacg
 6361 ttgtaaaacg acggccagtg ccatctgcag tgaataataa aatgtgtgtt tgtccgaaat
 6421 acgcgtttga gatttctgtc ccgactaaat tcatgtcgcg cgatagtggt gtttatcgcc
 6481 gatagagatg gcgatattgg aaaaatcgat atttgaaaat atggcatatt gaaaatgtcg
 6541 ccgatgtgag tttctgtgta actgatatcg ccatttttcc aaaagttgat ttttgggcat
 6601 acgcgatatc tggcgatacg cttatatcgt ttacggggga tggcgataga cgcctttggt
 6661 gacttgggcg attctgtgtg tcgcaaatat cgcagtttcg atataggtga cagacgatat
 6721 gaggctatat cgccgataga ggcgacatca agctggcaca tggccaatgc atatcgatct
 6781 atacattgaa tcaatattgg ccattagcca tattattcat tggttatata gcataaatca
 6841 atattggcta ttggccattg catacgttgt atccatatca taatatgtac atttatattg
 6901 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
 6961 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
 7021 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
 7081 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
 7141 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
 7201 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
 7261 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
 7321 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
 7381 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
 7441 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
 7501 taagcagagc tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg
 7561 acctccatag aagacaccgg gaccgatcca gcctccgcgg ccgggaacgg tgcattggaa
 7621 cgcggattcc ccgtgccaag agtgacgtaa gtaccgccta tagagtctat aggcccaccc
 7681 ccttggcttc ttatgcatgc tatactgttt ttggcttggg gtctatacac ccccgcttcc
 7741 tcatgttata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
 7801 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
 7861 actctcttta ttggctatat gccaatacac tgtccttcag agactgacac ggactctgta
 7921 tttttacagg atggggtctc atttattatt tacaaattca catatacaac accaccgtcc
 7981 ccagtgcccg cagtttttat taaacataac gtgggatctc cacgcgaatc tcgggtacgt
 8041 gttcc
//

载体图谱质粒图谱pdf版和相关资料下载

载体质粒应用举例

分享到:

全部载体分类

相关载体

相关文章

相关问答

Copyright © 20012-2013 BIOFENG. 生物风 版权所有 Powered by Biofeng 沪ICP备2021037978号-1
欢迎您的访问和咨询....