| 质粒名称: | GW1CMV-Perceval |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 21737 |
| 存储实验室: | Gary Yellen |
|
载体骨架基本信息 |
|
| 载体名称: | pGW1-CMV |
| 载体出品公司: | British Biotech |
| 载体抗性: | 氨苄霉素 |
| 空载体大小: | 5076 bp |
| 载体类型: | 哺乳细胞表达 |
| 高拷贝/低拷贝: | 未知 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Perceval (fluorescent ATP:ADP sensor) |
| 物种来源: | 合成 |
| 插入大小: | 1934 bp |
| 基因突变: | improved Kozak sequence |
| 标签/融合蛋白: | 5'UTR（N端） |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | HindIII(未破坏) |
| 3' 克隆位点: | EcoRI(未破坏) |
| 5' 测序引物 | EGFP-N (rev) gives 5' third |
| 3' 测序引物 | EGFP-C (fwd) gives middle third |
|
文献引用方法 |
|
| 材料和方法部分: | GW1CMV-Perceval was a gift from Gary Yellen (Addgene plasmid # 21737) |
| 参考文献部分: | A genetically encoded fluorescent reporter of ATP:ADP ratio. Berg J, Hung YP, Yellen G. Nat Methods. 2009 Feb . 6(2):161-6. 10.1038/nmeth.1288 PubMed 19122669 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS GW1CMV-Perceval Sequencing Result 7557 bp DNA SYN
DEFINITION GW1CMV-Perceval Sequencing Result
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..7557
/organism="GW1CMV-Perceval Sequencing Result"
/mol_type="other DNA"
promoter 241..817
/label="CMV_immearly_promoter"
misc_feature 320..607
/label="CAG_enhancer"
misc_feature 774..794
/label="CMV_fwd_primer"
promoter 775..844
/label="CMV_promoter"
promoter 787..906
/label="CMV2_promoter"
misc_feature 818..837
/label="pCEP_fwd_primer"
misc_feature 820..844
/label="LNCX_primer"
CDS complement(1312..1770)
/label="ORF frame 1"
CDS complement(1673..3751)
/label="ORF frame 3"
CDS 1991..3730
/label="ORF frame 2"
gene 2165..2434
/label="ECFP (444 - 713)"
/gene="ECFP (444 - 713)"
misc_feature 2171..2254
/label="M153T,V163A,S175G (Venus)"
misc_feature 2371..2392
/label="EGFP_C_primer"
gene 2453..2884
/label="ECFP (0 - 431)"
/gene="ECFP (0 - 431)"
gene 2453..2884
/label="EGFP (0 - 431)"
/gene="EGFP (0 - 431)"
misc_feature complement(2495..2516)
/label="EGFP_N_primer"
CDS complement(2767..3744)
/label="ORF frame 1"
terminator 3855..3986
/label="SV40_PA_terminator"
misc_feature 3943..3962
/label="EBV_rev_primer"
misc_feature complement(4022..4044)
/label="M13_pUC_rev_primer"
promoter complement(4058..4087)
/label="lac_promoter"
gene complement(5169..6029)
/label="Ampicillin"
/gene="Ampicillin"
CDS complement(5169..6029)
/label="ORF frame 2"
promoter complement(6640..6668)
/label="AmpR_promoter"
misc_feature complement(6827..6849)
/label="pGEX_3_primer"
misc_feature complement(7036..7055)
/label="SV40pro_F_primer"
misc_feature complement(7086..7229)
/label="lacZ_a"
misc_feature 7200..7222
/label="M13_pUC_fwd_primer"
promoter 7215..7231
/label="M13_forward20_primer"
ORIGIN
1 ATCGCAGTTT CGATATAGGT GACAGACGAT ATGAGGCTAT ATCGCCGATA GAGGCGACAT
61 CAAGCTGGCA CATGGCCAAT GCATATCGAT CTATACATTG AATCAATATT GGCCATTAGC
121 CATATTATTC ATTGGTTATA TAGCATAAAT CAATATTGGC TATTGGCCAT TGCATACGTT
181 GTATCCATAT CATAATATGT ACATTTATAT TGGCTCATGT CCAACATTAC CGCCATGTTG
241 ACATTGATTA TTGACTAGTT ATTAATAGTA ATCAATTACG GGGTCATTAG TTCATAGCCC
301 ATATATGGAG TTCCGCGTTA CATAACTTAC GGTAAATGGC CCGCCTGGCT GACCGCCCAA
361 CGACCCCCGC CCATTGACGT CAATAATGAC GTATGTTCCC ATAGTAACGC CAATAGGGAC
421 TTTCCATTGA CGTCAATGGG TGGAGTATTT ACGGTAAACT GCCCACTTGG CAGTACATCA
481 AGTGTATCAT ATGCCAAGTA CGCCCCCTAT TGACGTCAAT GACGGTAAAT GGCCCGCCTG
541 GCATTATGCC CAGTACATGA CCTTATGGGA CTTTCCTACT TGGCAGTACA TCTACGTATT
601 AGTCATCGCT ATTACCATGG TGATGCGGTT TTGGCAGTAC ATCAATGGGC GTGGATAGCG
661 GTTTGACTCA CGGGGATTTC CAAGTCTCCA CCCCATTGAC GTCAATGGGA GTTTGTTTTG
721 GCACCAAAAT CAACGGGACT TTCCAAAATG TCGTAACAAC TCCGCCCCAT TGACGCAAAT
781 GGGCGGTAGG CGTGTACGGT GGGAGGTCTA TATAAGCAGA GCTCGTTTAG TGAACCGTCA
841 GATCGCCTGG AGACGCCATC CACGCTGTTT TGACCTCCAT AGAAGACACC GGGACCGATC
901 CAGCCTCCGC GGCCGGGAAC GGTGCATTGG AACGCGGATT CCCCGTGCCA AGAGTGACGT
961 AAGTACCGCC TATAGAGTCT ATAGGCCCAC CCCCTTGGCT TCTTATGCAT GCTATACTGT
1021 TTTTGGCTTG GGGTCTATAC ACCCCCGCTT CCTCATGTTA TAGGTGATGG TATAGCTTAG
1081 CCTATAGGTG TGGGTTATTG ACCATTATTG ACCACTCCCC TATTGGTGAC GATACTTTCC
1141 ATTACTAATC CATAACATGG CTCTTTGCCA CAACTCTCTT TATTGGCTAT ATGCCAATAC
1201 ACTGTCCTTC AGAGACTGAC ACGGACTCTG TATTTTTACA GGATGGGGTC TCATTTATTA
1261 TTTACAAATT CACATATACA ACACCACCGT CCCCAGTGCC CGCAGTTTTT ATTAAACATA
1321 ACGTGGGATC TCCACGCGAA TCTCGGGTAC GTGTTCCGGA CATGGGCTCT TCTCCGGTAG
1381 CGGCGGAGCT TCTACATCCG AGCCCTGCTC CCATGCCTCC AGCGACTCAT GGTCGCTCGG
1441 CAGCTCCTTG CTCCTAACAG TGGAGGCCAG ACTTAGGCAC AGCACGATGC CCACCACCAC
1501 CAGTGTGCCG CACAAGGCCG TGGCGGTAGG GTATGTGTCT GAAAATGAGC TCGGGGAGCG
1561 GGCTTGCACC GCTGACGCAT TTGGAAGACT TAAGGCAGCG GCAGAAGAAG ATGCAGGCAG
1621 CTGAGTTGTT GTGTTCTGAT AAGAGTCAGA GGTAACTCCC GTTGCGGTGC TGTTAACGGT
1681 GGAGGGCAGT GTAGTCTGAG CAGTACTCGT TGCTGCCGCG CGCGCCACCA GACATAATAG
1741 CTGACAGACT AACAGACTGT TCCTTTCCAT GGGTCTTTTC TGCAGTCACC GTCCTTGACA
1801 CGAAGCTTCC CGGCCACAGC AATCGATCCA TGACACAACC AGTGACAAGC AGTCCCCCAG
1861 TGAAACCGCA TCCGCATCCG AGTCCGATAC CGATAAAGAT TCTGAATCGG AGTGAGTGCC
1921 GCGTCCGAGA GCGTTCCCTG TCCACGTCCA CCATCGGCGG AGCAGGTGTG CCTGAGGCCT
1981 CTAGACCACC ATGAAAAAGG TTGAATCCAT CATCAGGCCC GAAAAGCTGG AGATCGTTAA
2041 GAAGGCTCTC TCGGACGCTG GATATGTGGG TATGACAGTC TCTGAGGTCA AGGGCACGGG
2101 CGTCCAGGGC GGCATCGTCG AGAGGTACCG AGGAAGGGAG TACTCTGCAG GCTACAACAG
2161 CGACAACGTC TATATCACCG CCGACAAGCA GAAGAACGGC ATCAAGGCCA ACTTCAAGAT
2221 CCGCCACAAC ATCGAGGACG GCGGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC
2281 CATCGGCGAC GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCTTCC AGTCCAAGCT
2341 GAGCAAAGAC CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC
2401 CGGGATCACT CTCGGCATGG ACGAGCTGTA CAAGGGCGGT TCCGGAGGCA TGGTGAGCAA
2461 GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG GCGACGTAAA
2521 CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG GCAAGCTGAC
2581 CCTGAAGCTG ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC TCGTGACCAC
2641 CCTGGGCTAC GGCCTGCAGT GCTTCGCCCG CTACCCCGAC CACATGAAGC AGCACGACTT
2701 CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT TCAAGGACGA
2761 CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG TGAACCGCAT
2821 CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA AGCTGGAGTA
2881 CAACGGCACC ATAGTAGATC TGATCCCTAA GGTAAAAATT GAGCTCGTGG TGAAGGAGGA
2941 GGACGTCGAT AACGTGATAG ACATTATTTG CGAAAATGCC CGCACAGGTA ACCCCGGCGA
3001 CGGTAAGATA TTTGTGATCC CAGTGGAGCG AGTGGTCAGG GTGCGAACCA AAGAGGAGGG
3061 AGCATCTGGT GGTGGATCCG GTGGAGGAGG TGCGTCTGGT ATGAAAAAGG TGGAAGCTAT
3121 CATCAGACCC GAAAAGCTGG AGATCGTTAA AAAGGCTCTT TCCGACGCCG GATATGTGGG
3181 TATGACAGTC TCTGAGGTCA AAGGCCGCGG TGCAGGTGGA GGGGATCTGA TCCCTAAGGT
3241 AAAAATTGAG CTCGTGGTGA AGGAGGAGGA CGTCGATAAC GTGATAGACA TTATTTGCGA
3301 AAATGCCCGC ACAGGTAACC CCGGCGACGG TAAGATATTT GTGATCCCAG TGGAGCGAGT
3361 GGTCAGGGTG CGAACCAAAG AGGAGGGAGC ATCTGGTGGA GGTGGCGGTT CCGGAGGTGC
3421 ATCTGGTATG AAAAAGGTGG AAGCTATCAT CAGACCCGAA AAGCTGGAGA TCGTTAAAAA
3481 GGCTCTTTCC GACGCCGGAT ATGTGGGTAT GACAGTCTCT GAGGTCAAAG GCCGCGGTGC
3541 AGGTGGAGGG GATCTGATCC CTAAGGTAAA AATTGAGCTC GTGGTGAAGG AGGAGGACGT
3601 CGATAACGTG ATAGACATTA TTTGCGAAAA TGCCCGCACA GGTAACCCCG GCGACGGTAA
3661 GATATTTGTG ATCCCAGTGG AGCGAGTGGT CAGGGTGCGA ACCAAAGAGG AGGGAAAGGA
3721 AGCATTGTGA CTCGACGAAT TCATTGATCA TAATCAGCCA TACCACATTT GTAGAGGTTT
3781 TACTTGCTTT AAAAAACCTC CCACACCTCC CCCTGAACCT GAAACATAAA ATGAATGCAA
3841 TTGTTGTTGT TAACTTGTTT ATTGCAGCTT ATAATGGTTA CAAATAAAGC AATAGCATCA
3901 CAAATTTCAC AAATAAAGCA TTTTTTTCAC TGCATTCTAG TTGTGGTTTG TCCAAACTCA
3961 TCAATGTATC TTATCATGTC TGGATCCGTA CCGAGCTCGC GTAATCATGT CATAGCTGTT
4021 TCCTGTGTGA AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA
4081 GTGTAAAGCC TGGGGTGCCT AATGAGTGAG CTAACTACAT TAATTGCGTT GCGCTCACTG
4141 CCCGCTTTCC AGTCGGGAAA CCTGTCGTGC CAGCTGcatt aatgaatcgg ccaacgcgcg
4201 gggagaggcg gtttgcgtat tgggcgctCT TCCGCTTCCT CGCTCACTGA CTCGCTGCGC
4261 TCGGTCGTTC GGCTGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC
4321 ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG
4381 AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT
4441 CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG
4501 GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA
4561 TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG
4621 TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT
4681 CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC
4741 GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC
4801 GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT
4861 GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC
4921 GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC
4981 AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG
5041 AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG
5101 ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA GTAAACTTGG
5161 TCTGACAGTT ACCAATGCTT AATCAGTGAG GCACCTATCT CAGCGATCTG TCTATTTCGT
5221 TCATCCATAG TTGCCTGACT CCCCGTCGTG TAGATAACTA CGATACGGGA GGGCTTACCA
5281 TCTGGCCCCA GTGCTGCAAT GATACCGCGA GACCCACGCT CACCGGCTCC AGATTTATCA
5341 GCAATAAACC AGCCAGCCGG AAGGGCCGAG CGCAGAAGTG GTCCTGCAAC TTTATCCGCC
5401 TCCATCCAGT CTATTAATTG TTGCCGGGAA GCTAGAGTAA GTAGTTCGCC AGTTAATAGT
5461 TTGCGCAACG TTGTTGCCAT TGCTACAGGC ATCGTGGTGT CACGCTCGTC GTTTGGTATG
5521 GCTTCATTCA GCTCCGGTTC CCAACGATCA AGGCGAGTTA CATGATCCCC CATGTTGTGC
5581 AAAAAAGCGG TTAGCTCCTT CGGTCCTCCG ATCGTTGTCA GAAGTAAGTT GGCCGCAGTG
5641 TTATCACTCA TGGTTATGGC AGCACTGCAT AATTCTCTTA CTGTCATGCC ATCCGTAAGA
5701 TGCTTTTCTG TGACTGGTGA GTACTCAACC AAGTCATTCT GAGAATAGTG TATGCGGCGA
5761 CCGAGTTGCT CTTGCCCGGC GTCAATACGG GATAATACCG CGCCACATAG CAGAACTTTA
5821 AAAGTGCTCA TCATTGGAAA ACGTTCTTCG GGGCGAAAAC TCTCAAGGAT CTTACCGCTG
5881 TTGAGATCCA GTTCGATGTA ACCCACTCGT GCACCCAACT GATCTTCAGC ATCTTTTACT
5941 TTCACCAGCG TTTCTGGGTG AGCAAAAACA GGAAGGCAAA ATGCCGCAAA AAAGGGAATA
6001 AGGGCGACAC GGAAATGTTG AATACTCATA CTCTTCCTTT TTCAATAAGC GGCCGCGGCC
6061 ATGCCGGCCA CTAGTCTCGA GGGCCACCCC GGTTGATAAT CAGAAAAGCC CCAAAAACAG
6121 GAAGATTGTA TAAGCAAATA TTTAAATTGT AAGCGTTAAT ATTTTGTTAA AATTCGCGTT
6181 AAATTTTTGT TAAATCAGCT CATTTTTTAA CCAATAGGCC GAAATCGGCA AAATCCCTTA
6241 TAAATCAAAA GAATAGACCG AGATAGGGTT GAGTGTTGTT CCAGTTTGGA ACAAGAGTCC
6301 ACTATTAAAG AACGTGGACT CCAACGTCAA AGGGCGAAAA ACCGTCTATC AGGGCGATGG
6361 CCCACTACGT GAACCATCAC CCTAATCAAG TTTTTTGGGG TCGAGGTGCC GTAAAGCACT
6421 AAATCGGAAC CCTAAAGGGA GCCCCCGATT TAGAGCTTGA CGGGGAAAGC CGGCGAACGT
6481 GGCGAGAAAG GAAGGGAAGA AAGCGAAAGG AGCGGGCGCT AGGGCGCTGG CAAGTGTAGC
6541 GGTCACGCTG CGCGTAACCA CCACACCCGC CGCGCTTAAT GCGCCGCTAC AGGGCGCGTG
6601 GGGATCCCTC GAGTTATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA
6661 TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG
6721 TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT AACCTATAAA AATAGGCGTA
6781 TCACGAGGCC CTTTCGTCTC GCGCGTTTCG GTGATGACGG TGAAAACCTC TGACACATGC
6841 AGCTCCCGGA GACGGTCACA GCTTGTCTGT AAGCGGATGC CGGGAGCAGA CAAGCCCGTC
6901 AGGGCGCGTC AGCGGGTGTT GGCGGGTGTC GGGGCTGGCT TAACTATGCG GCATCAGAGC
6961 AGATTGTACT GAGAGTGCAC CATATGAAGA CGTCGCCTCC TCACTACTTC TGGAATAGCT
7021 CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG GGCGCCATTC
7081 GCCATTCAGG CTGCGCAACT GTTGGGAAGG GCGATCGGTG CGGGCCTCTT CGCTATTACG
7141 CCAGCTGGCG AAAGGGGGAT GTGCTGCAAG GCGATTAAGT TGGGTAACGC CAGGGTTTTC
7201 CCAGTCACGA CGTTGTAAAA CGACGGCCAG TGCCCGTGTC AAGGACGGTG ACTGCAGTGA
7261 ATAATAAAAT GTGTGTTTGT CCGAAATACG CGTTTTGAGA TTTCTGTCGC CGACTAAATT
7321 CATGTCGCGC GATAGTGGTG TTTATCGCCG ATAGAGATGG CGATATTGGA AAAATCGATA
7381 TTTGAAAATA TGGCATATTG AAAATGTCGC CGATGTGAGT TTCTGTGTAA CTGATATCGC
7441 CATTTTTCCA AAAGTGATTT TTGGGCATAC GCGATATCTG GCGATAGCGC TTATATCGTT
7501 TACGGGGGAT GGCGATAGAC GACTTTGGTG ACTTGGGCGA TTCTGTGTGT CGCAAAT
//
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