质粒名称: | pCAG-VSVG |
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出品公司: | Addgene |
目录编号: | 64084 |
质粒用途: | 水泡性口炎病毒印第安纳株糖蛋白基因的表达载体 |
存储实验室: | Ian Wickersham |
相关质粒: |
pCAG-VSVG pCAG-VSVL pCAG-VSVM pCAG-VSVN pCAG-VSVP |
载体骨架基本信息 |
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载体名称: | pCAGEN |
空载体大小: | 4795 bp |
完整质粒大小: | 6331 bp |
载体类型: | 哺乳细胞表达 |
载体抗性: | 氨苄霉素 |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | 高拷贝 |
插入基因信息 |
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插入基因名称: | VSV glycoprotein |
物种来源: | Vesicular Stomatitis Virus |
插入大小: | 1536 bp |
启动子: | CAG |
克隆方法: | 未知 |
5’测序引物 | CTCTAGAGCCTCTGCTAACc |
3’测序引物 | CAGAAGTCAGATGCTCAAG |
文献引用方法 |
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材料和方法部分: | pCAG-VSVG was a gift from Ian Wickersham (Addgene plasmid # 64084 ; http://n2t.net/addgene:64084 ; RRID:Addgene_64084) |
参考文献部分: | 未发表 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pCAG-VSVG 6331 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION Expression vector for Vesicular Stomatitis Virus Indiana strain glycoprotein gene. ACCESSION . VERSION . KEYWORDS pCAG-VSVG SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 6331) TITLE Genetic Neuroengineering Group Plasmids JOURNAL Unpublished REFERENCE 2 (bases 1 to 6331) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..6331 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 4..383 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 386..661 /label=chicken beta-actin promoter intron 662..1670 /label=chimeric intron /note="chimera between introns from chicken beta-actin and rabbit beta-globin" primer_bind 1594..1616 /label=pCAGGS-5 /note="Chimeric intron in CAG promoter, forward primer" primer_bind 1678..1697 /label=pCAG-F /note="Rabbit beta-globin intron, for pCAG plasmids, forward primer" CDS 1734..3269 /codon_start=1 /product="vesicular stomatitis virus G glycoprotein" /label=VSV-G /note="Indiana strain" /translation="MKCLLYLTFLFIGVNCKFTIVFPHNQKGNWKNVPSNYHYCPSSSD LNWHNDLIGTALQVKMPKSHKAIQADGWMCHASKWVTTCDFRWYGPKYITHSIRSFTPS VEQCKESIEQTKQGTWLNPGFPPQSCGYATVTDAEAVIVQVTPHHVLVDEYTGEWVDSQ FINGKCSNYICPTVHNSTTWHSDYKVKGLCDSNLISMDITFFSEDGELSSLGKEGTGFR SNYFAYETGGKACKMQYCKHWGVRLPSGVWFEMADKDLFAAARFPECPEGSSISAPSQT SVDVSLIQDVERILDYSLCQETWSKIRAGLPISPVDLSYLAPKNPGTGPAFTIINGTLK YFETRYIRVDIAAPILSRMVGMISGTTTERELWDDWAPYEDVEIGPNGVLRTSSGYKFP LYMIGHGMLDSDLHLSSKAQVFEHPHIQDAASQLPDDESLFFGDTGLSKNPIELVEGWF SSWKSSIASFFFIIGLIIGLFLVLRVGIHLCIKLKHTKKRQIYTDIEMNRLGK" primer_bind complement(3355..3374) /label=Bglob-pA-R /note="Rabbit beta-globin polyA region, reverse primer" polyA_signal 3420..3475 /label=-beta-globin poly(A) signal /note="rabbit beta-globin polyadenylation signal (Gil and Proudfoot, 1987)" primer_bind complement(3474..3493) /label=rbglobpA-R /note="Rabbit beta-globin polyA, reverse primer. Also called rb-glob-pA-term-R" primer_bind complement(3834..3850) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3834..3850) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3847..3869) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3858..3874 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3882..3912) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3927..3948 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." promoter 4007..4202 /label=SV40 promoter /note="SV40 early promoter" rep_origin 4053..4188 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4115..4134 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" polyA_signal 4208..4342 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(4245..4264) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 4299..4318 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(4410..4427) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(4581..5169) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(4661..4680) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(5340..6200) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5963..5982 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(6201..6305) /gene="bla" /label=AmpR promoter ORIGIN 1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata 61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac 241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc 421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca 481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg 541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt 601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc 661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc 721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc 781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag 841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg 901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg 961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc 1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg 1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc 1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc 1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg 1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct 1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg 1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc 1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt 1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg 1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg 1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca 1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatgaagt 1741 gccttttgta cttaaccttt ttattcattg gggtgaattg caagttcacc atagtttttc 1801 cacacaacca aaaaggaaac tggaaaaatg ttccttctaa ttaccattat tgcccgtcaa 1861 gctcagattt aaattggcat aatgacttaa taggcacagc cttacaagtc aaaatgccca 1921 agagtcacaa ggctattcaa gcagacggtt ggatgtgtca tgcttccaaa tgggtcacta 1981 cttgtgattt ccgctggtat ggaccgaagt atataacaca ttccatccga tccttcactc 2041 catctgtaga acaatgcaag gaaagcattg aacaaacgaa acaaggaact tggctgaatc 2101 caggcttccc tcctcaaagt tgtggatatg caactgtgac ggatgccgaa gcagtgattg 2161 tccaggtgac tcctcaccat gtgctggttg atgaatacac aggagaatgg gttgattcac 2221 agttcatcaa cggaaaatgc agcaattaca tatgccccac tgtccataac tctacaacct 2281 ggcattctga ctataaggtc aaagggctat gtgattctaa cctcatttcc atggacatca 2341 ccttcttctc agaggacgga gagctatcat ccctgggaaa ggagggcaca gggttcagaa 2401 gtaactactt tgcttatgaa actggaggca aggcctgcaa aatgcaatac tgcaagcatt 2461 ggggagtcag actcccatca ggtgtctggt tcgagatggc tgataaggat ctctttgctg 2521 cagccagatt ccctgaatgc ccagaagggt caagtatctc tgctccatct cagacctcag 2581 tggatgtaag tctaattcag gacgttgaga ggatcttgga ttattccctc tgccaagaaa 2641 cctggagcaa aatcagagcg ggtcttccaa tctctccagt ggatctcagc tatcttgctc 2701 ctaaaaaccc aggaaccggt cctgctttca ccataatcaa tggtacccta aaatactttg 2761 agaccagata catcagagtc gatattgctg ctccaatcct ctcaagaatg gtcggaatga 2821 tcagtggaac taccacagaa agggaactgt gggatgactg ggcaccatat gaagacgtgg 2881 aaattggacc caatggagtt ctgaggacca gttcaggata taagtttcct ttatacatga 2941 ttggacatgg tatgttggac tccgatcttc atcttagctc aaaggctcag gtgttcgaac 3001 atcctcacat tcaagacgct gcttcgcaac ttcctgatga tgagagttta ttttttggtg 3061 atactgggct atccaaaaat ccaatcgagc ttgtagaagg ttggttcagt agttggaaaa 3121 gctctattgc ctcttttttc tttatcatag ggttaatcat tggactattc ttggttctcc 3181 gagttggtat ccatctttgc attaaattaa agcacaccaa gaaaagacag atttatacag 3241 acatagagat gaaccgactt ggaaagtaag cggccgcact cctcaggtgc aggctgccta 3301 tcagaaggtg gtggctggtg tggccaatgc cctggctcac aaataccact gagatctttt 3361 tccctctgcc aaaaattatg gggacatcat gaagcccctt gagcatctga cttctggcta 3421 ataaaggaaa tttattttca ttgcaatagt gtgttggaat tttttgtgtc tctcactcgg 3481 aaggacatat gggagggcaa atcatttaaa acatcagaat gagtatttgg tttagagttt 3541 ggcaacatat gccatatgct ggctgccatg aacaaaggtg gctataaaga ggtcatcagt 3601 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt 3661 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct 3721 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc 3781 ctcttctctt atgaagatcc ctcgacctgc agcccaagct tggcgtaatc atggtcatag 3841 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc 3901 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc 3961 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc ggatccgcat ctcaattagt 4021 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 4081 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 4141 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 4201 aaaagctaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa 4261 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa 4321 tgtatcttat catgtctgga tccgctgcat taatgaatcg gccaacgcgc ggggagaggc 4381 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt 4441 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca 4501 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa 4561 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat 4621 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc 4681 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc 4741 gcctttctcc cttcgggaag cgtggcgctt tctcaatgct cacgctgtag gtatctcagt 4801 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac 4861 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg 4921 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca 4981 gagttcttga agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc 5041 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa 5101 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa 5161 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac 5221 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta 5281 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt 5341 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata 5401 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc 5461 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac 5521 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag 5581 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac 5641 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc 5701 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg 5761 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc 5821 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct 5881 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc 5941 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc 6001 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc 6061 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc 6121 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca 6181 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt 6241 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt 6301 ccgcgcacat ttccccgaaa agtgccacct g //
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