出品公司: | -- |
---|---|
载体名称: | pYM39-EYFP |
质粒类型: | 酵母系列,毕赤酵母表达载体 |
终止子: | ADH1 |
复制子: | ori |
启动子: | TEF |
载体大小: | 4946bp |
5' 测序引物及序列: | T7:TAATACGACTCACTATAGGG |
3' 测序引物及序列: | -- |
载体标签: | EYFP |
载体抗性: | 氨苄青霉素 |
筛选标记: | -- |
克隆菌株: | DH5α 等 |
宿主细胞(系): | 酵母细胞 |
备注: | -- |
产品目录号: | -- |
稳定性: | -- |
组成型/诱导型: | -- |
病毒/非病毒: | -- |
买家导航
pYM39-EYFP载体 pYM39EYFP质粒基本信息
pYM39-EYFP质粒图谱 pYM39EYFP载体图谱和pYM39-EYFP载体序列 pYM39EYFP质粒序列多克隆位点信息
pYM39-EYFP质粒 pYM39EYFP载体简介
pYM39-EYFP质粒序列 pYM39EYFP载体序列
LOCUS pYM39-EYFP 4946 bp ds-DNA circular SYN 19-SEP-2016 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS Untitled 10 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4946) AUTHORS . TITLE Direct Submission JOURNAL Exported Monday, September 19, 2016 from SnapGene Viewer 3.2.1 http://www.biofeng.com/ FEATURES Location/Qualifiers source 1..4946 /organism="synthetic DNA construct" /mol_type="other DNA" CDS 79..795 /codon_start=1 /product="enhanced YFP" /note="EYFP" /note="mammalian codon-optimized" /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK FICTTGKLPVPWPTLVTTFGYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLLE FVTAAGITLGMDELYK" CDS 802..828 /codon_start=1 /product="HA (human influenza hemagglutinin) epitope tag" /note="HA" /translation="YPYDVPDYA" terminator 861..1048 /gene="S. cerevisiae ADH1" /note="ADH1 terminator" /note="transcription terminator for the S. cerevisiae alcohol dehydrogenase 1 (ADH1) gene" gene 1123..2479 /note="kanMX" /note="yeast selectable marker conferring kanamycin resistance (Wach et al., 1994)" promoter 1123..1466 /note="TEF promoter" /note="Ashbya gossypii TEF promoter" CDS 1467..2276 /codon_start=1 /gene="aph(3')-Ia" /product="aminoglycoside phosphotransferase" /note="KanR" /note="confers resistance to kanamycin" /translation="MGKEKTHVSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" terminator 2282..2479 /note="TEF terminator" /note="Ashbya gossypii TEF terminator" promoter complement(2584..2602) /note="T7 promoter" /note="promoter for bacteriophage T7 RNA polymerase" rep_origin complement(2860..3448) /direction=LEFT /note="ori" /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" CDS complement(3619..4479) /codon_start=1 /gene="bla" /product="beta-lactamase" /note="AmpR" /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" promoter complement(4480..4584) /gene="bla" /note="AmpR promoter" promoter 4930..2 /note="SP6 promoter" /note="promoter for bacteriophage SP6 RNA polymerase" ORIGIN 1 gaacgcggcc gccagctgaa gcttcgtacg ctgcaggtcg acggatccgg agcaggtgct 61 ggtgctggtg ctggagcaat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc 121 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag 181 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc 241 gtgccctggc ccaccctcgt gaccaccttc ggctacggcg tgcagtgctt cgcccgctac 301 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag 361 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc 421 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc 481 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc 541 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc 601 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg 661 ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc caacgagaag 721 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac 781 gagctgtaca agtaaggatc ctatccatat gacgttccag attacgctgc tcagtgctag 841 ggcgcgccac ttctaaataa gcgaatttct tatgatttat gatttttatt attaaataag 901 ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat 961 tcttattctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagtatgagg 1021 tcgctcttat tgaccacacc tctaccggca gatccgctag ggataacagg gtaatataga 1081 tctgtttagc ttgcctcgtc cccgccgggt cacccggcca gcgacatgga ggcccagaat 1141 accctccttg acagtcttga cgtgcgcagc tcaggggcat gatgtgactg tcgcccgtac 1201 atttagccca tacatcccca tgtataatca tttgcatcca tacattttga tggccgcacg 1261 gcgcgaagca aaaattacgg ctcctcgctg cagacctgcg agcagggaaa cgctcccctc 1321 acagacgcgt tgaattgtcc ccacgccgcg cccctgtaga gaaatataaa aggttaggat 1381 ttgccactga ggttcttctt tcatatactt ccttttaaaa tcttgctagg atacagttct 1441 cacatcacat ccgaacataa acaaccatgg gtaaggaaaa gactcacgtt tcgaggccgc 1501 gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg 1561 ggcaatcagg tgcgacaatc tatcgattgt atgggaagcc cgatgcgcca gagttgtttc 1621 tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact 1681 ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg 1741 catggttact caccactgcg atccccggca aaacagcatt ccaggtatta gaagaatatc 1801 ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga 1861 ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat 1921 cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc 1981 ctgttgaaca agtctggaaa gaaatgcata agcttttgcc attctcaccg gattcagtcg 2041 tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt 2101 gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga 2161 actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg 2221 ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag 2281 tactgacaat aaaaagattc ttgttttcaa gaacttgtca tttgtatagt ttttttatat 2341 tgtagttgtt ctattttaat caaatgttag cgtgatttat attttttttc gcctcgacat 2401 catctgccca gatgcgaagt taagtgcgca gaaagtaata tcatgcgtca atcgtatgtg 2461 aatgctggtc gctatactgc tgtcgattcg atactaacgc cgccatccag tttaaacgag 2521 ctcgaattca tcgatgatat cagatccact agtggcctat gcggccgcgg atctgccggt 2581 ctccctatag tgagtcgtat taatttcgat aagccaggtt aacctgcatt aatgaatcgg 2641 ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga 2701 ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat 2761 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca 2821 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc 2881 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata 2941 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc 3001 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcaatgctc 3061 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga 3121 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc 3181 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag 3241 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag 3301 gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag 3361 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca 3421 gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga 3481 cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat 3541 cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga 3601 gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg 3661 tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga 3721 gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc 3781 agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac 3841 tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc 3901 agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc 3961 gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc 4021 catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt 4081 ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc 4141 atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg 4201 tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag 4261 cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat 4321 cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc 4381 atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa 4441 aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta 4501 ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa 4561 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga 4621 aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct 4681 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac 4741 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt 4801 tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac tgagagtgca 4861 ccatatggac atattgtcgt tagaacgcgg ctacaattaa tacataacct tatgtatcat 4921 acacatacga tttaggtgac actata //
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