pCDH-EF1-MCS-T2A-copGFP

载体介绍：
Stable overexpression with cDNA lentivectors
Strong and ubiquitous expression of the gene of interest
Single or double expression cassette with choice of reporter gene
Target gene expressed from CMV, EF1, or MSCV promoter
Choose from FIV- or HIV-based vectors
Gene expression from constitutive promoters
The multiple cloning site (MCS) located downstream of an internal promoter allows convenient cloning of your gene of interest.


优点：
Reliable delivery to dividing or non-dividing cells
When used with lentiviral packaging plasmids, your cDNA constructs can be packaged into VSV-G pseudotyped viral particles and delivered into a wide range of mammalian cells with high efficiency. Stable cell lines can be generated that express the gene of interest. The system can also be used to overexpress genes in model organisms.

Convenient selection of transduced cells
In addition to the pCD-MCS one-promoter vectors, which feature the Multiple Cloning Site (MCS) downstream of the CMV promoter, the pCD system offers the option of a second expression cassette downstream of the MCS to express the puromycin resistance gene or copGFP as a reporter under the control of a constitutive human elongation factor 1α (EF1) promoter, which is functional in most cell lines.

Efficient coexpression of target and reporter with T2A peptide
SBI’s most recent additions to the cDNA vector collection confront the problems of promoter interference and imbalanced expression by incorporating a “self-cleaving” T2A peptide derived from the insect virus Thosea asigna to mediate coexpression of a reporter gene with the target cDNA. This allows an easy way to track cells actively expressing your gene of interest.

The CD52X EF1 or CMV Promoter Series with T2A Co-expressed markers
SBI’s cDNA expression vectors using the EF1 promoter (CD520A-1, CD521A-1) or CMV (CD524A-1) driving expression also incorporates the 2A-like sequence (T2A) from the insect virus Thosea asigna to mediate the co-expression of a reporter gene with the target cDNA. Reporter genes have been cloned at either the first or second positions relative to the T2A element, and both markers achieved high expression levels at either position.