| 质粒名称: | mCherry-Rab5A Q79L |
|---|---|
| 存储实验室: | Biofeng Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3 |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5446 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Rab5A |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 647 bp |
| 基因突变: | Q79L |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | mCherry on N-term |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | mCherry-Rab5A Q79L was a gift from Biofeng Lab。 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS mcherry-Rab5A Q79L 6745 bp ds-DNA circular SYN 29-JUN-2022
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6745)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Wednesday, Jun 29, 2022 from SnapGene Viewer 5.1.4.1 to
Vector NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|989539200|
COMMENT VNTDBDATE|989539200|
COMMENT VNTNAME|mcherry-Rab5A Q79L|
FEATURES Location/Qualifiers
enhancer 237..616
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 617..820
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 865..883
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
regulatory 897..906
/regulatory_class="other"
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 903..1610
/codon_start=1
/product="monomeric derivative of DsRed fluorescent protein
(Shaner et al., 2004)"
/label=mCherry
/note="mammalian codon-optimized"
misc_feature 1617..2264
/label=Rab5A Q79L
promoter complement(2300..2318)
/label=SP6 promoter
/note="promoter for bacteriophage SP6 RNA polymerase"
polyA_signal 2344..2568
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2614..3042
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 3056..3385
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 3236..3371
/label=SV40 ori
/note="SV40 origin of replication"
CDS 3452..4246
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
polyA_signal 4420..4541
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4590..4606)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4614..4630
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4638..4668)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4683..4704
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4992..5580)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5751..6611)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
promoter complement(6612..6716)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
61 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
421 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
481 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
541 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
601 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
661 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
721 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
781 cggtaggcgt gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc
841 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttgcca
901 ccatggtgag caagggcgag gaggataaca tggccatcat caaggagttc atgcgcttca
961 aggtgcacat ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg
1021 gccgccccta cgagggcacc cagaccgcca agctgaaggt gaccaagggt ggccccctgc
1081 ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc
1141 accccgccga catccccgac tacttgaagc tgtccttccc cgagggcttc aagtgggagc
1201 gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg
1261 acggcgagtt catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg
1321 taatgcagaa gaagaccatg ggctgggagg cctcctccga gcggatgtac cccgaggacg
1381 gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa ggacggcggc cactacgacg
1441 ctgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc gcctacaacg
1501 tcaacatcaa gttggacatc acctcccaca acgaggacta caccatcgtg gaacagtacg
1561 aacgcgccga gggccgccac tccaccggcg gcatggacga gctgtacaag ggatccatgg
1621 ctagtcgagg cgcaacaaga cccaacgggc caaatactgg aaataaaata tgccagttca
1681 aactagtact tctgggagag tccgctgttg gcaaatcaag cctagtgctt cgttttgtga
1741 aaggccaatt tcatgaattt caagagagta ccattggggc tgcttttcta acccaaactg
1801 tatgtcttga tgacactaca gtaaagtttg aaatatggga tacagctggt ctagaacgat
1861 accatagcct agcaccaatg tactacagag gagcacaagc agccatagtt gtatatgata
1921 tcacaaatga ggagtccttt gcaagagcaa aaaattgggt taaagaactt cagaggcaag
1981 caagtcctaa cattgtaata gctttatcgg gaaacaaggc cgacctagca aataaaagag
2041 cagtagattt ccaggaagca cagtcctatg cagatgacaa tagtttatta ttcatggaga
2101 catccgctaa aacatcaatg aatgtaaatg aaatattcat ggcaatagct aaaaaattgc
2161 caaagaatga accacaaaat ccaggagcaa attctgccag aggaagagga gtagacctta
2221 ccgaacccac acaaccaacc aggaatcagt gttgtagtaa ctgaggcggc cgctcgagca
2281 tgcatctaga gggccctatt ctatagtgtc acctaaatgc tagagctcgc tgatcagcct
2341 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
2401 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
2461 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
2521 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
2581 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
2641 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
2701 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
2761 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
2821 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
2881 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
2941 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
3001 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
3061 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
3121 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
3181 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
3241 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
3301 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
3361 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
3421 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
3481 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
3541 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
3601 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
3661 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
3721 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
3781 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
3841 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
3901 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
3961 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
4021 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
4081 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
4141 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
4201 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
4261 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
4321 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
4381 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
4441 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
4501 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
4561 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
4621 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
4681 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
4741 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
4801 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4861 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4921 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4981 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
5041 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
5101 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
5161 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
5221 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
5281 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
5341 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
5401 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
5461 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
5521 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
5581 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
5641 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
5701 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
5761 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
5821 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
5881 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
5941 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
6001 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
6061 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
6121 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
6181 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
6241 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
6301 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
6361 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
6421 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
6481 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
6541 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
6601 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
6661 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
6721 tttccccgaa aagtgccacc tgacg
//
载体图谱质粒图谱pdf版和相关资料下载
载体质粒应用举例
- 上一篇：pYPQ-dzCas9-Act3.0
- 下一篇：pRB895
