质粒名称: | p11L2w |
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出品公司: | Addgene |
目录编号: | 37331 |
存储实验室: | John Schiller |
载体骨架基本信息 |
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载体名称: | custom |
载体出品公司: | -- |
空载体大小: | -- |
完整质粒大小: | 5974 bp |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | -- |
载体抗性: | 卡那霉素,50μg/mL |
生长温度: | 37 ℃ |
宿主菌: | DH5a |
拷贝数: | -- |
插入基因信息 |
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插入基因名称: | HPV11 L2 |
物种来源: | H. sapiens (human) |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子: | EF1a |
标签/融合蛋白: | -- |
克隆方法: | -- |
5’克隆位点: | -- |
3’克隆位点: | -- |
5’测序引物 | -- |
3’测序引物 | -- |
文献引用方法 |
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材料和方法部分: | p11L2w was a gift from John Schiller (Addgene plasmid # 37331 ; http://n2t.net/addgene:37331 ; RRID:Addgene_37331) |
参考文献部分: | Factors influencing subcellular localization of the human papillomavirus L2 minor structural protein. Kieback E, Muller M. Virology. 2006 Feb 5;345(1):199-208. Epub 2005 Oct 27. 10.1016/j.virol.2005.09.047 PubMed 16257028 |
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载体序列
LOCUS p11L2w 5974 bp ds-DNA circular SYN 12-MAY-2021 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS p11L2w SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5974) AUTHORS Kieback E, Muller M TITLE Factors influencing subcellular localization of the human papillomavirus L2 minor structural protein. JOURNAL Virology. 2006 Feb 5;345(1):199-208. Epub 2005 Oct 27. PUBMED 16257028 REFERENCE 2 (bases 1 to 5974) AUTHORS . TITLE Direct Submission JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58 http://www.biofeng.com FEATURES Location/Qualifiers source 1..5974 /organism="synthetic DNA construct" /mol_type="other DNA" promoter 113..1291 /label=EF-1-alpha promoter /note="strong constitutive promoter for human elongation factor EF-1-alpha" intron 344..1282 /label=EF-1-alpha intron A /note="intron upstream of the start codon of human EF-1-alpha" primer_bind 1239..1259 /label=EF1a-F /note="Human elongation factor-1a promoter, forward primer" misc_feature 2791..3379 /label=WPRE /note="woodchuck hepatitis virus posttranscriptional regulatory element" primer_bind complement(2844..2864) /label=WPRE-R /note="WPRE, reverse primer" CDS complement(3262..3273) /codon_start=1 /product="Factor Xa recognition and cleavage site" /label=Factor Xa site /translation="IEGR" promoter complement(3401..3418) /label=T3 promoter /note="promoter for bacteriophage T3 RNA polymerase" polyA_signal 3436..3557 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3447..3466) /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind 3501..3520 /label=SV40pA-R /note="SV40 polyA, reverse primer" rep_origin 3745..4333 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4234..4253 /label=pBR322ori-F /note="pBR322 origin, forward primer" primer_bind complement(4417..4437) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 4422..4751 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 4602..4737 /label=SV40 ori /note="SV40 origin of replication" primer_bind 4664..4683 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" promoter 4799..4846 /label=EM7 promoter /note="synthetic bacterial promoter " promoter 4838..4856 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" CDS 4866..5660 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(4920..4939) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 5530..5549 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(5718..5737) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 5762..5809 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" ORIGIN 1 ccgctaccgg actcagatct cgagctcaag cttgcaaaga tggataaagt tttaaacaga 61 gaggaatctt tgcagctaat ggaccttgta ggtcttgaaa ggagtgggaa ttggctccgg 121 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 181 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 241 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 301 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 361 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 421 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 481 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 541 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 601 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 661 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 721 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 781 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 841 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 901 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 961 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1021 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1081 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1141 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1201 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1261 aagttttttt cttccatttc aggtgtcgtg aggaattctc tagagatccc tcgacctcga 1321 gatccatcct gtacggaagt gttacttctg ctctaaaagc tgcggaattg tacccgcggc 1381 cgctctagat gaagcccaga gcccggcgga gaaagcgggc cagcgccacc cagctgtacc 1441 agacctgcaa ggccaccggc acctgccccc ctgacgtgat ccccaaggtg gagcacacca 1501 ccatcgccga ccagatcctg aagtggggca gcctgggcgt gttcttcggc ggcctgggca 1561 tcggcaccgg cgccggcagc ggcggccggg ccggctacat ccccctgggc agcagcccta 1621 agcccgccat caccgggggc cctgccgcca gaccccccgt gctggtggag cctgtggccc 1681 ccagcgaccc cagcatcgtg agtctgatcg aggagagcgc catcatcaac gccggcgccc 1741 ctgaggtggt gcctcctacc cagggcggct tcaccatcac cagcagcgag agcaccaccc 1801 ctgccatcct ggacgtgagc gtgaccaacc acaccaccac cagcgtgttc cagaaccccc 1861 tgttcaccga gcctagcgtg atccagcccc agcctcccgt ggaggccagc ggccacatcc 1921 tgatcagcgc ccctaccatc accagccagc acgtggagga catccccctg gacaccttcg 1981 tggtgagcag cagcgacagc ggccccacca gcagcacccc tctgccccgg gccttcccca 2041 gacctcgggt gggcctgtac agcagagccc tgcagcaggt gcaggtgacc gaccccgcct 2101 tcctgagcac cccccagcgg ctggtgacct acgacaaccc tgtgtacgag ggcgaggacg 2161 tgagcctgca gttcacccac gagagcatcc acaacgcccc cgacgaggcc ttcatggata 2221 tcatcagact gcaccggccc gccatcacca gcagacgggg cctggtgcgc ttcagcagaa 2281 tcggccagcg gggcagcatg tacacccgga gcggccagca catcggcgcc agaattcact 2341 acttccagga catcagccct gtgacccagg ccgccgagga gatcgagctg caccccctgg 2401 tggccgccga gaacgacacc ttcgacatct acgccgagcc cttcgaccct atccccgacc 2461 ccgtgcagca cagcgtgacc cagagctacc tgaccagcac ccctaacacc ctgagccaga 2521 gctggggcaa caccaccgtg cccctgagca tccccagcga ctggttcgtg cagagcggcc 2581 ccgacatcac cttccctacc gctagcatgg gcaccccctt cagccccgtg acccctgccc 2641 tgcccaccgg ccccgtgttc atcaccggca gcgacttcta cctgcaccct acctggtact 2701 tcgcccggcg gagacggaag cggatccccc tgttcttcac cgacgtggcc gcctaagctt 2761 atcgataccg tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa 2821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta 2881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa 2941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg 3001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc 3061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc 3121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg 3181 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg 3241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg 3301 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc 3361 ctttgggccg cctccccgca tcgataccgt cggccgcttc cctttagtga gggttaatgc 3421 ttcgagcaga catgataaga tacattgatg agtttggaca aaccacaact agaatgcagt 3481 gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta accattataa 3541 gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag gttcaggggg 3601 aggtgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtatg gaattcttaa 3661 ttaactagcc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc 3721 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt 3781 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac 3841 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt 3901 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct 3961 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga 4021 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac 4081 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg 4141 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt 4201 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc 4261 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg 4321 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc 4381 ttttgctcac atgttcttaa ttaagctgta cactgtggaa tgtgtgtcag ttagggtgtg 4441 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag 4501 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc 4561 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc 4621 ccagttccgc ccattctccg ccccatgact gactaatttt ttttatttat gcagaggccg 4681 aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag 4741 gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt 4801 tgacaattaa tcatcggcat agtatatcgg catagtataa tacgactcac tataggaggg 4861 ccaccatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 4921 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 4981 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 5041 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 5101 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 5161 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 5221 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 5281 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 5341 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 5401 ccgacggcga ggatctcgtc gtgacacatg gcgatgcctg cttgccgaat atcatggtgg 5461 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 5521 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 5581 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 5641 ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc aagcgaattc 5701 gctagaggga ggctaactga aacacggaag gagacaatac cggaaggaac ccgcgctatg 5761 acggcaataa aaagacagaa taaaacgcac ggtgttgggt cgtttgttca taaacgcggg 5821 gttcggtccc agggctggca ctctgtcgat accccaccga gaccccattg gggccaatac 5881 gcccgcgttt cttccttttc cccaccccac cccccaagtt cgggtgaagg cccagggctc 5941 gcagccaacg tcggggcggc aggccctgcc atag //
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