质粒名称: | sfGFP-N1 |
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出品公司: | 爱迪基因 |
目录编号: | 54737 |
存储实验室: | Michael Davidson, Geoffrey Waldo |
载体骨架基本信息 |
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载体名称: | sfGFP-N1 |
载体出品公司: | -- |
载体抗性: | 卡那霉素 |
空载体大小: | 4750 bp |
完整质粒大小: | -- |
载体修饰: | -- |
载体类型: | 哺乳细胞表达 |
筛选标记: | 新霉素(G418) |
高拷贝/低拷贝: | 高拷贝 |
生长条件: | 37 ℃ |
插入基因信息 |
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插入基因名称: | -- |
物种来源: | -- |
插入大小: | -- |
基因突变: | -- |
GenBank编号 | -- |
启动子 | -- |
标签/融合蛋白: | sfGFP(主干上的C终端) |
克隆方法: | -- |
5' 克隆位点: | -- |
3' 克隆位点: | -- |
5' 测序引物 | -- |
3' 测序引物 | -- |
文献引用方法 |
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材料和方法部分: | sfGFP-N1 was a gift from Michael Davidson & Geoffrey Waldo (Addgene plasmid # 54737 ; http://n2t.net/addgene:54737 ; RRID:Addgene_54737) |
参考文献部分: | Engineering and characterization of a superfolder green fluorescent protein. Pédelacq JD, Cabantous S, Tran T, Terwilliger TC, Waldo GS. Nat Biotechnol. 2006 Jan . 24(1):79-88. 10.1038/nbt1172 PubMed 16369541 |
买家导航
sfGFPN1载体基本信息
sfGFPN1载体图谱质粒图谱和多克隆位点信息
sfGFPN1载体简介
sfGFPN1载体序列
LOCUS sfGFP-N1 4732 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION Localization: N1 Cloning Vector, Excitation: 485, Emission: 507. ACCESSION . VERSION . KEYWORDS sfGFP-N1 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 4732) TITLE Michael Davidson Empty Backbones REFERENCE 2 (bases 1 to 4732) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..4732 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 1..304 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 305..508 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 459..479 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" misc_feature 531..611 /label=MCS /note="multiple cloning site" regulatory 613..622 /regulatory_class="other" /note="vertebrate consensus sequence for strong initiation of translation (Kozak, 1987)" CDS 619..1338 /codon_start=1 /product="enhanced CFP with monomerizing A206K mutation (Zacharias et al., 2002)" /label=mECFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" CDS 619..1338 /codon_start=1 /product="enhanced CFP" /label=ECFP /note="mammalian codon-optimized" /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDD GTYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIK ANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLL EFVTAAGITHGMDELYK" primer_bind complement(664..685) /label=EGFP-N /note="EGFP, reverse primer" primer_bind 1272..1293 /label=EGFP-C /note="EGFP, forward primer" polyA_signal 1460..1581 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(1497..1516) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 1551..1570 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" rep_origin complement(1588..2043) /direction=LEFT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1725..1746) /label=F1ori-F /note="F1 origin, forward primer" primer_bind 1937..1956 /label=F1ori-R /note="F1 origin, reverse primer" promoter 2070..2174 /gene="bla" /label=AmpR promoter promoter 2176..2533 /label=SV40 promoter /note="SV40 enhancer and early promoter" primer_bind complement(2199..2219) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" rep_origin 2384..2519 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2446..2465 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2568..3362 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2622..2641) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3232..3251 /label=Neo-F /note="Neomycin resistance gene, forward primer" primer_bind complement(3550..3569) /label=TK-pA-R /note="Thymidine kinase polyA, reverse primer" polyA_signal 3594..3641 /label=HSV TK poly(A) signal /note="herpes simplex virus thymidine kinase polyadenylation signal (Cole and Stacy, 1985)" rep_origin 3970..4558 /direction=RIGHT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind 4459..4478 /label=pBR322ori-F /note="pBR322 origin, forward primer" ORIGIN 1 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 61 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 121 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 181 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 241 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 301 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 361 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 421 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 481 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 541 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 601 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 661 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc 721 gagggcgatg ccaccaacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 781 cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc 841 taccccgacc acatgaagcg ccacgacttc ttcaagtccg ccatgcccga aggctacgtc 901 caggagcgca ccatcagctt caaggacgac ggcacctaca agacccgcgc cgaggtgaag 961 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1021 ggcaacatcc tggggcacaa gctggagtac aacttcaaca gccacaacgt ctatatcacc 1081 gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa cgtggaggac 1141 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 1201 ctgctgcccg acaaccacta cctgagcacc cagtccgtgc tgagcaaaga ccccaacgag 1261 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tcacggcatg 1321 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatttgt 1381 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat 1441 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa 1501 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 1561 caaactcatc aatgtatctt aaggcgtaaa ttgtaagcgt taatattttg ttaaaattcg 1621 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 1681 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 1741 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 1801 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 1861 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 1921 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 1981 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 2041 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa 2101 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt 2161 gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg 2221 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc 2281 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca 2341 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc 2401 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc 2461 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct 2521 aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag 2581 atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 2641 cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 2701 cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag 2761 cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 2821 ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 2881 ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 2941 cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 3001 gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 3061 tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg 3121 tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 3181 gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 3241 cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 3301 gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 3361 gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 3421 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 3481 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg 3541 gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat 3601 aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc 3661 ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt 3721 ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa 3781 cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt 3841 aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac 3901 caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa 3961 aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc 4021 accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt 4081 aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc cgtagttagg 4141 ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc 4201 agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt 4261 accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga 4321 gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct 4381 tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg 4441 cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca 4501 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa 4561 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt 4621 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc attagttatt 4681 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cg //