| 质粒名称: | pUC119 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 50010 |
| 存储实验室: | Joachim Messing |
|
载体骨架基本信息 |
|
| 载体名称: | pUC19 |
| 载体出品公司: | Messing Lab |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | 从HgiAI位点（5465）到DraI位点（5941）的M13的IG区域插入到pUC的唯一NdeI位点（2499）。M13-IG区域的取向使得被包装成ssDNA的lac区域与M13mp载体中的相同。 |
| 载体类型: | 克隆载体 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | M13-F20 |
| 3' 测序引物 | M13R |
|
文献引用方法 |
|
| 材料和方法部分: | pUC119 was a gift from Joachim Messing (Addgene plasmid # 50010 ; http://n2t.net/addgene:50010 ; RRID:Addgene_50010) |
| 参考文献部分: | Production of single-stranded plasmid DNA. Vieira J, Messing J. Methods Enzymol. 1987;153:3-11. PubMed 3323803 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pUC119 3162 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION pUC cloning vector.
ACCESSION .
VERSION .
KEYWORDS pUC119
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3162)
AUTHORS Vieira J, Messing J
TITLE Production of single-stranded plasmid DNA.
JOURNAL Methods Enzymol. 1987;153:3-11.
PUBMED 3323803
REFERENCE 2 (bases 1 to 3162)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..3162
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind 107..128
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 143..173
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 181..197
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 186..208
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 205..221
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 205..221
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
CDS 217..543
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSLHACRSTLEDPRVPSSNSLAVVLQRRDWENPGVTQLNR
LAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISHRIRQSNHSTRP
VAAH"
misc_feature 234..290
/label=MCS
/note="pUC18/19 multiple cloning site"
primer_bind complement(291..308)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(291..307)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(300..322)
/label=M13/pUC Forward
/note="In lacZ gene"
rep_origin 520..975
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(607..626)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 817..838
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(992..1011)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 1111..1133
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(1171..1189)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1257..1361
/gene="bla"
/label=AmpR promoter
CDS 1362..2222
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(1580..1599)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 2393..2981
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 2882..2901
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3135..3152
/label=L4440
/note="L4440 vector, forward primer"
ORIGIN
1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
241 catgcctgca ggtcgactct agaggatccc cgggtaccga gctcgaattc actggccgtc
301 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
361 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
421 cagttgcgca gcctgaatgg cgaatggcgc ctgatgcggt attttctcct tacgcatctg
481 tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt agcggcgcat
541 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
601 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
661 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
721 ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga tagacggttt
781 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
841 caacactcaa ccctatctcg ggctattctt ttgatttata agggattttg ccgatttcgg
901 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
961 taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
1021 gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg
1081 catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac
1141 cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt ttataggtta
1201 atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga aatgtgcgcg
1261 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
1321 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
1381 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
1441 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
1501 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
1561 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
1621 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
1681 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
1741 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
1801 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
1861 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
1921 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
1981 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
2041 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
2101 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
2161 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
2221 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
2281 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
2341 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
2401 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
2461 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
2521 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
2581 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
2641 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
2701 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
2761 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
2821 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
2881 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
2941 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
3001 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
3061 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
3121 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga ag
//
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