| 质粒名称: | pWY82 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 65721 |
| 存储实验室: | James Birchler |
|
载体骨架基本信息 |
|
| 载体名称: | pTF101.1 |
| 载体抗性: | 观霉素和链霉素 |
| 空载体大小: | 9189 bp |
| 完整质粒大小: | 11656 bp |
| 载体类型: | 植物表达 |
| 筛选标记: | Basta |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Telomere |
| 物种来源: | A. thaliana (mustard weed) |
| 插入大小: | 2500 bp |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | SpeI,EcoRI(未破坏) |
| 3' 克隆位点: | NcoI,EcoRV(未破坏) |
| 5' 测序引物 | gggcgacgtcgactgttta |
| 3' 测序引物 | TCACACAGGAAACAGCTATGAC |
|
文献引用方法 |
|
| 材料和方法部分: | pWY82 was a gift from James Birchler (Addgene plasmid # 65721 ; http://n2t.net/addgene:65721 ; RRID:Addgene_65721) |
| 参考文献部分: | Telomere-mediated chromosomal truncation in maize. Yu W, Lamb JC, Han F, Birchler JA. Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17331-6. Epub 2006 Nov 3. 10.1073/pnas.0605750103 PubMed 17085598 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pWY82 11656 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Plasmid with 2.5 kb of telomere sequence.
ACCESSION .
VERSION .
KEYWORDS pWY82
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 11656)
AUTHORS Yu W, Lamb JC, Han F, Birchler JA
TITLE Telomere-mediated chromosomal truncation in maize.
JOURNAL Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17331-6. Epub 2006 Nov
3.
PUBMED 17085598
REFERENCE 2 (bases 1 to 11656)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..11656
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1187..1816
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2250..3317
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
MRYRNLIEGEASAGS"
rep_origin 3383..3577
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(3813..3835)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3921..4061
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 3976..3995
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4076..4093)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4247..4835)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4327..4346)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5079..5870)
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
misc_feature 6398..6422
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
primer_bind 6622..6644
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6636..6653
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6637..6653
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS complement(7223..7771)
/codon_start=1
/gene="Streptomyces hygroscopicus bar"
/product="phosphinothricin acetyltransferase"
/label=BlpR
/note="confers resistance to bialophos or phosphinothricin"
/translation="MSPERRPADIRRATEADMPAVCTIVNHYIETSTVNFRTEPQEPQE
WTDDLVRLRERYPWLVAEVDGEVAGIAYAGPWKARNAYDWTAESTVYVSPRHQRTGLGS
TLYTHLLKSLEAQGFKSVVAVIGLPNDPSVRMHEALGYAPRGMLRAAGFKHGNWHDVGF
WQLDFSLPVPPRPVLPVTEI"
promoter complement(7923..8267)
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind complement(7959..7979)
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
primer_bind 8369..8388
/label=T7
/note="T7 promoter, forward primer"
promoter 8369..8387
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
promoter complement(11212..11229)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(11247..11263)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(11247..11263)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(11260..11282)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 11271..11287
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(11295..11325)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 11340..11361
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_feature 11519..11543
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 agtactttga tccaacccct ccgctgctat agtgcagtcg gcttctgacg ttcagtgcag
61 ccgtcttctg aaaacgacat gtcgcacaag tcctaagtta cgcgacaggc tgccgccctg
121 cccttttcct ggcgttttct tgtcgcgtgt tttagtcgca taaagtagaa tacttgcgac
181 tagaaccgga gacattacgc catgaacaag agcgccgccg ctggcctgct gggctatgcc
241 cgcgtcagca ccgacgacca ggacttgacc aaccaacggg ccgaactgca cgcggccggc
301 tgcaccaagc tgttttccga gaagatcacc ggcaccaggc gcgaccgccc ggagctggcc
361 aggatgcttg accacctacg ccctggcgac gttgtgacag tgaccaggct agaccgcctg
421 gcccgcagca cccgcgacct actggacatt gccgagcgca tccaggaggc cggcgcgggc
481 ctgcgtagcc tggcagagcc gtgggccgac accaccacgc cggccggccg catggtgttg
541 accgtgttcg ccggcattgc cgagttcgag cgttccctaa tcatcgaccg cacccggagc
601 gggcgcgagg ccgccaaggc ccgaggcgtg aagtttggcc cccgccctac cctcaccccg
661 gcacagatcg cgcacgcccg cgagctgatc gaccaggaag gccgcaccgt gaaagaggcg
721 gctgcactgc ttggcgtgca tcgctcgacc ctgtaccgcg cacttgagcg cagcgaggaa
781 gtgacgccca ccgaggccag gcggcgcggt gccttccgtg aggacgcatt gaccgaggcc
841 gacgccctgg cggccgccga gaatgaacgc caagaggaac aagcatgaaa ccgcaccagg
901 acggccagga cgaaccgttt ttcattaccg aagagatcga ggcggagatg atcgcggccg
961 ggtacgtgtt cgagccgccc gcgcacgtct caaccgtgcg gctgcatgaa atcctggccg
1021 gtttgtctga tgccaagctg gcggcctggc cggccagctt ggccgctgaa gaaaccgagc
1081 gccgccgtct aaaaaggtga tgtgtatttg agtaaaacag cttgcgtcat gcggtcgctg
1141 cgtatatgat gcgatgagta aataaacaaa tacgcaaggg gaacgcatga aggttatcgc
1201 tgtacttaac cagaaaggcg ggtcaggcaa gacgaccatc gcaacccatc tagcccgcgc
1261 cctgcaactc gccggggccg atgttctgtt agtcgattcc gatccccagg gcagtgcccg
1321 cgattgggcg gccgtgcggg aagatcaacc gctaaccgtt gtcggcatcg accgcccgac
1381 gattgaccgc gacgtgaagg ccatcggccg gcgcgacttc gtagtgatcg acggagcgcc
1441 ccaggcggcg gacttggctg tgtccgcgat caaggcagcc gacttcgtgc tgattccggt
1501 gcagccaagc ccttacgaca tatgggccac cgccgacctg gtggagctgg ttaagcagcg
1561 cattgaggtc acggatggaa ggctacaagc ggcctttgtc gtgtcgcggg cgatcaaagg
1621 cacgcgcatc ggcggtgagg ttgccgaggc gctggccggg tacgagctgc ccattcttga
1681 gtcccgtatc acgcagcgcg tgagctaccc aggcactgcc gccgccggca caaccgttct
1741 tgaatcagaa cccgagggcg acgctgcccg cgaggtccag gcgctggccg ctgaaattaa
1801 atcaaaactc atttgagtta atgaggtaaa gagaaaatga gcaaaagcac aaacacgcta
1861 agtgccggcc gtccgagcgc acgcagcagc aaggctgcaa cgttggccag cctggcagac
1921 acgccagcca tgaagcgggt caactttcag ttgccggcgg aggatcacac caagctgaag
1981 atgtacgcgg tacgccaagg caagaccatt accgagctgc tatctgaata catcgcgcag
2041 ctaccagagt aaatgagcaa atgaataaat gagtagatga attttagcgg ctaaaggagg
2101 cggcatggaa aatcaagaac aaccaggcac cgacgccgtg gaatgcccca tgtgtggagg
2161 aacgggcggt tggccaggcg taagcggctg ggttgtctgc cggccctgca atggcactgg
2221 aacccccaag cccgaggaat cggcgtgacg gtcgcaaacc atccggcccg gtacaaatcg
2281 gcgcggcgct gggtgatgac ctggtggaga agttgaaggc cgcgcaggcc gcccagcggc
2341 aacgcatcga ggcagaagca cgccccggtg aatcgtggca agcggccgct gatcgaatcc
2401 gcaaagaatc ccggcaaccg ccggcagccg gtgcgccgtc gattaggaag ccgcccaagg
2461 gcgacgagca accagatttt ttcgttccga tgctctatga cgtgggcacc cgcgatagtc
2521 gcagcatcat ggacgtggcc gttttccgtc tgtcgaagcg tgaccgacga gctggcgagg
2581 tgatccgcta cgagcttcca gacgggcacg tagaggtttc cgcagggccg gccggcatgg
2641 ccagtgtgtg ggattacgac ctggtactga tggcggtttc ccatctaacc gaatccatga
2701 accgataccg ggaagggaag ggagacaagc ccggccgcgt gttccgtcca cacgttgcgg
2761 acgtactcaa gttctgccgg cgagccgatg gcggaaagca gaaagacgac ctggtagaaa
2821 cctgcattcg gttaaacacc acgcacgttg ccatgcagcg tacgaagaag gccaagaacg
2881 gccgcctggt gacggtatcc gagggtgaag ccttgattag ccgctacaag atcgtaaaga
2941 gcgaaaccgg gcggccggag tacatcgaga tcgagctagc tgattggatg taccgcgaga
3001 tcacagaagg caagaacccg gacgtgctga cggttcaccc cgattacttt ttgatcgatc
3061 ccggcatcgg ccgttttctc taccgcctgg cacgccgcgc cgcaggcaag gcagaagcca
3121 gatggttgtt caagacgatc tacgaacgca gtggcagcgc cggagagttc aagaagttct
3181 gtttcaccgt gcgcaagctg atcgggtcaa atgacctgcc ggagtacgat ttgaaggagg
3241 aggcggggca ggctggcccg atcctagtca tgcgctaccg caacctgatc gagggcgaag
3301 catccgccgg ttcctaatgt acggagcaga tgctagggca aattgcccta gcaggggaaa
3361 aaggtcgaaa aggtctcttt cctgtggata gcacgtacat tgggaaccca aagccgtaca
3421 ttgggaaccg gaacccgtac attgggaacc caaagccgta cattgggaac cggtcacaca
3481 tgtaagtgac tgatataaaa gagaaaaaag gcgatttttc cgcctaaaac tctttaaaac
3541 ttattaaaac tcttaaaacc cgcctggcct gtgcataact gtctggccag cgcacagccg
3601 aagagctgca aaaagcgcct acccttcggt cgctgcgctc cctacgcccc gccgcttcgc
3661 gtcggcctat cgcggccgct ggccgctcaa aaatggctgg cctacggcca ggcaatctac
3721 cagggcgcgg acaagccgcg ccgtcgccac tcgaccgccg gcgcccacat caaggcaccc
3781 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
3841 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
3901 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
3961 actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
4021 aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
4081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
4141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
4201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
4261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
4321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
4381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
4441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
4501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
4561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
4621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
4681 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
4741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
4801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
4861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg catgatatat
4921 ctcccaattt gtgtagggct tattatgcac gcttaaaaat aataaaagca gacttgacct
4981 gatagtttgg ctgtgagcaa ttatgtgctt agtgcatcta atcgcttgag ttaacgccgg
5041 cgaagcggcg tcggcttgaa cgaatttcta gctagacatt atttgccgac taccttggtg
5101 atctcgcctt tcacgtagtg gacaaattct tccaactgat ctgcgcgcga ggccaagcga
5161 tcttcttctt gtccaagata agcctgtcta gcttcaagta tgacgggctg atactgggcc
5221 ggcaggcgct ccattgccca gtcggcagcg acatccttcg gcgcgatttt gccggttact
5281 gcgctgtacc aaatgcggga caacgtaagc actacatttc gctcatcgcc agcccagtcg
5341 ggcggcgagt tccatagcgt taaggtttca tttagcgcct caaatagatc ctgttcagga
5401 accggatcaa agagttcctc cgccgctgga cctaccaagg caacgctatg ttctcttgct
5461 tttgtcagca agatagccag atcaatgtcg atcgtggctg gctcgaagat acctgcaaga
5521 atgtcattgc gctgccattc tccaaattgc agttcgcgct tagctggata acgccacgga
5581 atgatgtcgt cgtgcacaac aatggtgact tctacagcgc ggagaatctc gctctctcca
5641 ggggaagccg aagtttccaa aaggtcgttg atcaaagctc gccgcgttgt ttcatcaagc
5701 cttacggtca ccgtaaccag caaatcaata tcactgtgtg gcttcaggcc gccatccact
5761 gcggagccgt acaaatgtac ggccagcaac gtcggttcga gatggcgctc gatgacgcca
5821 actacctctg atagttgagt cgatacttcg gcgatcaccg cttcccccat gatgtttaac
5881 tttgttttag ggcgactgcc ctgctgcgta acatcgttgc tgctccataa catcaaacat
5941 cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg taccccaaaa
6001 aaacatgtca taacaagaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
6061 ggtcaaggtt ctggaccagt tgcgtgacgg cagttacgct acttgcatta cagcttacga
6121 accgaacgag gcttatgtcc actgggttcg tgcccgaatt gatcacaggc agcaacgctc
6181 tgtcatcgtt acaatcaaca tgctaccctc cgcgagatca tccgtgtttc aaacccggca
6241 gcttagttgc cgttcttccg aatagcatcg gtaacatgag caaagtctgc cgccttacaa
6301 cggctctccc gctgacgccg tcccggactg atgggctgcc tgtatcgagt ggtgattttg
6361 tgccgagctg ccggtcgggg agctgttggc tggctggtgg caggatatat tgtggtgtaa
6421 acaaattgac gcttagacaa cttaataaca cattgcggac gtttttaatg tactgaatta
6481 acgccgaatt gctctagcat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg
6541 tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa
6601 gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc
6661 taattcttca agacgtgctc aaatcactat ttccacaccc ctatatttct attgcactcc
6721 cttttaactg ttttttatta caaaaatgcc ctggaaaatg cactcccttt ttgtgtttgt
6781 ttttttgtga aacgatgttg tcaggtaatt tatttgtcag tctactatgg tggcccatta
6841 tattaatagc aactgtcggt ccaatagacg acgtcgattt tctgcatttg tttaaccacg
6901 tggattttat gacattttat attagttaat ttgtaaaacc tacccaatta aagacctcat
6961 atgttctaaa gactaatact taatgataac aattttcttt tagtgaagaa agggataatt
7021 agtaaatatg gaacaagggc agaagattta ttaaagccgc gtaagagaca acaagtaggt
7081 acgtggagtg tcttaggtga cttacccaca taacataaag tgacattaac aaacatagct
7141 aatgctccta tttgaatagt gcatatcagc ataccttatt acatatagat aggagcaaac
7201 tctagctaga ttgttgagag cagatctcgg tgacgggcag gaccggacgg ggcggtaccg
7261 gcaggctgaa gtccagctgc cagaaaccca cgtcatgcca gttcccgtgc ttgaagccgg
7321 ccgcccgcag catgccgcgg ggggcatatc cgagcgcctc gtgcatgcgc acgctcgggt
7381 cgttgggcag cccgatgaca gcgaccacgc tcttgaagcc ctgtgcctcc agggacttca
7441 gcaggtgggt gtagagcgtg gagcccagtc ccgtccgctg gtggcggggg gagacgtaca
7501 cggtcgactc ggccgtccag tcgtaggcgt tgcgtgcctt ccaggggccc gcgtaggcga
7561 tgccggcgac ctcgccgtcc acctcggcga cgagccaggg atagcgctcc cgcagacgga
7621 cgaggtcgtc cgtccactcc tgcggttcct gcggctcggt acggaagttg accgtgcttg
7681 tctcgatgta gtggttgacg atggtgcaga ccgccggcat gtccgcctcg gtggcacggc
7741 ggatgtcggc cgggcgtcgt tctgggctca tggtagatcc cccgttcgta aatggtgaaa
7801 attttcagaa aattgctttt gctttaaaag aaatgattta aattgctgca atagaagtag
7861 aatgcttgat tgcttgagat tcgtttgttt tgtatatgtt gtgttgagaa ttaattctcg
7921 aggtcctctc caaatgaaat gaacttcctt atatagagga agggtcttgc gaaggatagt
7981 gggattgtgc gtcatccctt acgtcagtgg agatatcaca tcaatccact tgctttgaag
8041 acgtggttgg aacgtcttct ttttccacga tgctcctcgt gggtgggggt ccatctttgg
8101 gaccactgtc ggcagaggca tcttcaacga tggcctttcc tttatcgcaa tgatggcatt
8161 tgtaggagcc accttccttt tccactatct tcacaataaa gtgacagata gctgggcaat
8221 ggaatccgag gaggtttccg gatattaccc tttgttgaaa agtctcaatt gccctttggt
8281 cttctgagac tgtatctttg atatttttgg agtagacaag cgtgtcgtgc tccaccatgt
8341 tgacctgcag gcatgcaagc tcaagcttta atacgactca ctatagggag atgttaaccg
8401 cggtccctag gccggccgct agccatttaa atgcatgctg cagcccgggc gacgtcgact
8461 gtttaaacga ggccttaatt aagggcccaa ttggccacgt acgtctagat ctggcgcgcc
8521 tcgaggatcc atggccgcgg gattccctcg ggtcccacag cgctttattt agggtttagg
8581 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
8641 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
8701 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
8761 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
8821 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
8881 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
8941 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
9001 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
9061 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
9121 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
9181 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
9241 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
9301 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
9361 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
9421 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
9481 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
9541 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
9601 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
9661 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
9721 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
9781 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
9841 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
9901 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
9961 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
10021 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
10081 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
10141 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
10201 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
10261 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
10321 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
10381 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
10441 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
10501 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
10561 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
10621 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
10681 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
10741 agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg
10801 tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt ttagggttta
10861 gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag ggtttagggt
10921 ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt tagggtttag
10981 ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg gtttagggtt
11041 tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt agggtttagg
11101 gtttagggtt tagggtttag ggtttagggt ttagggttta gggtttaggg tttagggttt
11161 agggtgacct gcagcccaag ctctcgggaa atcactagtg atcgatatct ccctttagtg
11221 agggttaatg tgaattcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
11281 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
11341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
11401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
11461 ggagcttgag cttggatcag attgtcgttt cccgccttca gtttaaacta tcagtgtttg
11521 acaggatata ttggcgggta aacctaagag aaaagagcgt ttattagaat aacggatatt
11581 taaaagggcg tgaaaaggtt tatccgttcg tccatttgta tgtgcatgcc aaccacaggg
11641 ttcccctcgg gatcaa
//
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