| 质粒名称: | pfNL43-dE-EGFP |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 36865 |
| 存储实验室: | Mario Chin |
|
载体骨架基本信息 |
|
| 载体名称: | pUC18 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2686 bp |
| 完整质粒大小: | 12880 bp |
| 载体修饰: | KasI site was knocked out. |
| 载体类型: | 哺乳动物表达，慢病毒，逆转录病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 30 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | fNL43-dE-EGFP |
| 物种来源: | HIV-I |
| 插入大小: | 10194 bp |
| 基因突变: | 在原NL43的6350个核苷酸后插入EGFP，它可以分裂/灭活病毒包膜 |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pfNL43-dE-EGFP was a gift from Mario Chin (Addgene plasmid # 36865 ; http://n2t.net/addgene:36865 ; RRID:Addgene_36865) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pfNL43-dE-EGFP 12880 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pfNL43-dE-EGFP
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12880)
TITLE NLdDIS
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 12880)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12880
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 69..326
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
CDS 482..1984
/codon_start=1
/gene="gag"
/product="gag protein from human immunodeficiency virus 1"
/label=HIV-1 gag
/translation="MGARASVLSGGELDKWEKIRLRPGGKKQYKLKHIVWASRELERFA
VNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTIAVLYCVHQRIDVKDTKEALDKI
EEEQNKSKKKAQQAAADTGNNSQVSQNYPIVQNLQGQMVHQAISPRTLNAWVKVVEEKA
FSPEVIPMFSALSEGATPQDLNTMLNTVGGHQAAMQMLKETINEEAAEWDRLHPVHAGP
IAPGQMREPRGSDIAGTTSTLQEQIGWMTHNPPIPVGEIYKRWIILGLNKIVRMYSPTS
ILDIRQGPKEPFRDYVDRFYKTLRAEQASQEVKNWMTETLLVQNANPDCKTILKALGPG
ATLEEMMTACQGVGGPGHKARVLAEAMSQVTNPATIMIQKGNFRNQRKTVKCFNCGKEG
HIAKNCRAPRKKGCWKCGKEGHQMKDCTERQANFLGKIWPSHKGRPGNFLQSRPEPTAP
PEESFRFGEETTTPSQKQEPIDKELYPLASLRSLFGSDPSSQ"
CDS 1777..4788
/codon_start=1
/gene="pol"
/product="pol protein from human immunodeficiency virus 1"
/label=HIV-1 pol
/translation="FFREDLAFPQGKAREFSSEQTRANSPTRRELQVWGRDNNSLSEAG
ADRQGTVSFSFPQITLWQRPLVTIKIGGQLKEALLDTGADDTVLEEMNLPGRWKPKMIG
GIGGFIKVGQYDQILIEICGHKAIGTVLVGPTPVNIIGRNLLTQIGCTLNFPISPIETV
PVKLKPGMDGPKVKQWPLTEEKIKALVEICTEMEKEGKISKIGPENPYNTPVFAIKKKD
STKWRKLVDFRELNKRTQDFWEVQLGIPHPAGLKQKKSVTVLDVGDAYFSVPLDKDFRK
YTAFTIPSINNETPGIRYQYNVLPQGWKGSPAIFQCSMTKILEPFRKQNPDIVIYQYMD
DLYVGSDLEIGQHRTKIEELRQHLLRWGFTTPDKKHQKEPPFLWMGYELHPDKWTVQPI
VLPEKDSWTVNDIQKLVGKLNWASQIYAGIKVRQLCKLLRGTKALTEVVPLTEEAELEL
AENREILKEPVHGVYYDPSKDLIAEIQKQGQGQWTYQIYQEPFKNLKTGKYARMKGAHT
NDVKQLTEAVQKIATESIVIWGKTPKFKLPIQKETWEAWWTEYWQATWIPEWEFVNTPP
LVKLWYQLEKEPIIGAETFYVDGAANRETKLGKAGYVTDRGRQKVVPLTDTTNQKTELQ
AIHLALQDSGLEVNIVTDSQYALGIIQAQPDKSESELVSQIIEQLIKKEKVYLAWVPAH
KGIGGNEQVDGLVSAGIRKVLFLDGIDKAQEEHEKYHSNWRAMASDFNLPPVVAKEIVA
SCDKCQLKGEAMHGQVDCSPGIWQLDCTHLEGKVILVAVHVASGYIEAEVIPAETGQET
AYFLLKLAGRWPVKTVHTDNGSNFTSTTVKAACWWAGIKQEFGIPYNPQSQGVIESMNK
ELKKIIGQVRDQAEHLKTAVQMAVFIHNFKRKGGIGGYSAGERIVDIIATDIQTKELQK
QITKIQNFRVYYRDSRDPVWKGPAKLLWKGEGAVVIQDNSDIKVVPRRKAKIIRDYGKQ
MAGDDCVASRQDED"
misc_feature 4473..4590
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
regulatory 6060..6069
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 6066..6782
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITLGMDELYK"
primer_bind complement(6111..6132)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(6372..6391)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 6719..6740
/label=EGFP-C
/note="EGFP, forward primer"
CDS 8060..8086
/codon_start=1
/product="nuclear export signal from the HIV Rev protein
(Fischer et al., 1995)"
/label=NES
/translation="LPPLERLTL"
LTR 8621..9254
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(10243..10260)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(10414..11002)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(10494..10513)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(11173..12033)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 11796..11815
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(12034..12138)
/gene="bla"
/label=AmpR promoter
primer_bind 12206..12224
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
primer_bind complement(12262..12284)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 12384..12403
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
ORIGIN
1 ccggagtact acaaagactg ctgacatcga gctttctaca agggactttc cgctggggac
61 tttccaggga ggtgtggcct gggcgggact ggggagtggc gagccctcag atgctacata
121 taagcagctg ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga
181 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
241 caaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
301 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaagta
361 aagccagagg agatctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
421 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
481 gatgggtgcg agagcgtcgg tattaagcgg gggagaatta gataaatggg aaaaaattcg
541 gttaaggcca gggggaaaga aacaatataa actaaaacat atagtatggg caagcaggga
601 gctagaacga ttcgcagtta atcctggcct tttagagaca tcagaaggct gtagacaaat
661 actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa
721 tacaatagca gtcctctatt gtgtgcatca aaggatagat gtaaaagaca ccaaggaagc
781 cttagataag atagaggaag agcaaaacaa aagtaagaaa aaggcacagc aagcagcagc
841 tgacacagga aacaacagcc aggtcagcca aaattaccct atagtgcaga acctccaggg
901 gcaaatggta catcaggcca tatcacctag aactttaaat gcatgggtaa aagtagtaga
961 agagaaggct ttcagcccag aagtaatacc catgttttca gcattatcag aaggagccac
1021 cccacaagat ttaaatacca tgctaaacac agtgggggga catcaagcag ccatgcaaat
1081 gttaaaagag accatcaatg aggaagctgc agaatgggat agattgcatc cagtgcatgc
1141 agggcctatt gcaccaggcc agatgagaga accaagggga agtgacatag caggaactac
1201 tagtaccctt caggaacaaa taggatggat gacacataat ccacctatcc cagtaggaga
1261 aatctataaa agatggataa tcctgggatt aaataaaata gtaagaatgt atagccctac
1321 cagcattctg gacataagac aaggaccaaa ggaacccttt agagactatg tagaccgatt
1381 ctataaaact ctaagagccg agcaagcttc acaagaggta aaaaattgga tgacagaaac
1441 cttgttggtc caaaatgcga acccagattg taagactatt ttaaaagcat tgggaccagg
1501 agcgacacta gaagaaatga tgacagcatg tcagggagtg gggggacccg gccataaagc
1561 aagagttttg gctgaagcaa tgagccaagt aacaaatcca gctaccataa tgatacagaa
1621 aggcaatttt aggaaccaaa gaaagactgt taagtgtttc aattgtggca aagaagggca
1681 catagccaaa aattgcaggg cccctaggaa aaagggctgt tggaaatgtg gaaaggaagg
1741 acaccaaatg aaagattgta ctgagagaca ggctaatttt ttagggaaga tctggccttc
1801 ccacaaggga aggccaggga attttcttca gagcagacca gagccaacag ccccaccaga
1861 agagagcttc aggtttgggg aagagacaac aactccctct cagaagcagg agccgataga
1921 caaggaactg tatcctttag cttccctcag atcactcttt ggcagcgacc cctcgtcaca
1981 ataaagatag gggggcaatt aaaggaagct ctattagata caggagcaga tgatacagta
2041 ttagaagaaa tgaatttgcc aggaagatgg aaaccaaaaa tgataggggg aattggaggt
2101 tttatcaaag taggacagta tgatcagata ctcatagaaa tctgcggaca taaagctata
2161 ggtacagtat tagtaggacc tacacctgtc aacataattg gaagaaatct gttgactcag
2221 attggctgca ctttaaattt tcccattagt cctattgaga ctgtaccagt aaaattaaag
2281 ccaggaatgg atggcccaaa agttaaacaa tggccattga cagaagaaaa aataaaagca
2341 ttagtagaaa tttgtacaga aatggaaaag gaaggaaaaa tttcaaaaat tgggcctgaa
2401 aatccataca atactccagt atttgccata aagaaaaaag acagtactaa atggagaaaa
2461 ttagtagatt tcagagaact taataagaga actcaagatt tctgggaagt tcaattagga
2521 ataccacatc ctgcagggtt aaaacagaaa aaatcagtaa cagtactgga tgtgggcgat
2581 gcatattttt cagttccctt agataaagac ttcaggaagt atactgcatt taccatacct
2641 agtataaaca atgagacacc agggattaga tatcagtaca atgtgcttcc acagggatgg
2701 aaaggatcac cagcaatatt ccagtgtagc atgacaaaaa tcttagagcc ttttagaaaa
2761 caaaatccag acatagtcat ctatcaatac atggatgatt tgtatgtagg atctgactta
2821 gaaatagggc agcatagaac aaaaatagag gaactgagac aacatctgtt gaggtgggga
2881 tttaccacac cagacaaaaa acatcagaaa gaacctccat tcctttggat gggttatgaa
2941 ctccatcctg ataaatggac agtacagcct atagtgctgc cagaaaagga cagctggact
3001 gtcaatgaca tacagaaatt agtgggaaaa ttgaattggg caagtcagat ttatgcaggg
3061 attaaagtaa ggcaattatg taaacttctt aggggaacca aagcactaac agaagtagta
3121 ccactaacag aagaagcaga gctagaactg gcagaaaaca gggagattct aaaagaaccg
3181 gtacatggag tgtattatga cccatcaaaa gacttaatag cagaaataca gaagcagggg
3241 caaggccaat ggacatatca aatttatcaa gagccattta aaaatctgaa aacaggaaaa
3301 tatgcaagaa tgaagggtgc ccacactaat gatgtgaaac aattaacaga ggcagtacaa
3361 aaaatagcca cagaaagcat agtaatatgg ggaaagactc ctaaatttaa attacccata
3421 caaaaggaaa catgggaagc atggtggaca gagtattggc aagccacctg gattcctgag
3481 tgggagtttg tcaatacccc tcccttagtg aagttatggt accagttaga gaaagaaccc
3541 ataataggag cagaaacttt ctatgtagat ggggcagcca atagggaaac taaattagga
3601 aaagcaggat atgtaactga cagaggaaga caaaaagttg tccccctaac ggacacaaca
3661 aatcagaaga ctgagttaca agcaattcat ctagctttgc aggattcggg attagaagta
3721 aacatagtga cagactcaca atatgcattg ggaatcattc aagcacaacc agataagagt
3781 gaatcagagt tagtcagtca aataatagag cagttaataa aaaaggaaaa agtctacctg
3841 gcatgggtac cagcacacaa aggaattgga ggaaatgaac aagtagatgg gttggtcagt
3901 gctggaatca ggaaagtact atttttagat ggaatagata aggcccaaga agaacatgag
3961 aaatatcaca gtaattggag agcaatggct agtgatttta acctaccacc tgtagtagca
4021 aaagaaatag tagccagctg tgataaatgt cagctaaaag gggaagccat gcatggacaa
4081 gtagactgta gcccaggaat atggcagcta gattgtacac atttagaagg aaaagttatc
4141 ttggtagcag ttcatgtagc cagtggatat atagaagcag aagtaattcc agcagagaca
4201 gggcaagaaa cagcatactt cctcttaaaa ttagcaggaa gatggccagt aaaaacagta
4261 catacagaca atggcagcaa tttcaccagt actacagtta aggccgcctg ttggtgggcg
4321 gggatcaagc aggaatttgg cattccctac aatccccaaa gtcaaggagt aatagaatct
4381 atgaataaag aattaaagaa aattatagga caggtaagag atcaggctga acatcttaag
4441 acagcagtac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
4501 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
4561 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca
4621 gtttggaaag gaccagcaaa gctcctctgg aaaggtgaag gggcagtagt aatacaagat
4681 aatagtgaca taaaagtagt gccaagaaga aaagcaaaga tcatcaggga ttatggaaaa
4741 cagatggcag gtgatgattg tgtggcaagt agacaggatg aggattaaca catggaaaag
4801 attagtaaaa caccatatgt atatttcaag gaaagctaag gactggtttt atagacatca
4861 ctatgaaagt actaatccaa aaataagttc agaagtacac atcccactag gggatgctaa
4921 attagtaata acaacatatt ggggtctgca tacaggagaa agagactggc atttgggtca
4981 gggagtctcc atagaatgga ggaaaaagag atatagcaca caagtagacc ctgacctagc
5041 agaccaacta attcatctgc actattttga ttgtttttca gaatctgcta taagaaatac
5101 catattagga cgtatagtta gtcctaggtg tgaatatcaa gcaggacata acaaggtagg
5161 atctctacag tacttggcac tagcagcatt aataaaacca aaacagataa agccaccttt
5221 gcctagtgtt aggaaactga cagaggacag atggaacaag ccccagaaga ccaagggcca
5281 cagagggagc catacaatga atggacacta gagcttttag aggaacttaa gagtgaagct
5341 gttagacatt ttcctaggat atggctccat aacttaggac aacatatcta tgaaacttac
5401 ggggatactt gggcaggagt ggaagccata ataagaattc tgcaacaact gctgtttatc
5461 catttcagaa ttgggtgtcg acatagcaga ataggcgtta ctcgacagag gagagcaaga
5521 aatggagcca gtagatccta gactagagcc ctggaagcat ccaggaagtc agcctaaaac
5581 tgcttgtacc aattgctatt gtaaaaagtg ttgctttcat tgccaagttt gtttcatgac
5641 aaaagcctta ggcatctcct atggcaggaa gaagcggaga cagcgacgaa gagctcatca
5701 gaacagtcag actcatcaag cttctctatc aaagcagtaa gtagtacatg taatgcaacc
5761 tataatagta gcaatagtag cattagtagt agcaataata atagcaatag ttgtgtggtc
5821 catagtaatc atagaatata ggaaaatatt aagacaaaga aaaatagaca ggttaattga
5881 tagactaata gaaagagcag aagacagtgg caatgagagt gaaggagaag tatcagcact
5941 tgtggagatg ggggtggaaa tggggcacca tgctccttgg gatattgatg atctgtagtg
6001 ctacagaaaa attgtgggtc acagtctatt atggggtacc taaggcctat ccacctgtcg
6061 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
6121 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
6181 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
6241 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
6301 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
6361 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
6421 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
6481 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
6541 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
6601 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
6661 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
6721 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
6781 aggacgagct gtaagctagc aaattaagag aacaatttgg aaataataaa acaataatct
6841 ttaagcaatc ctcaggaggg gacccagaaa ttgtaacgca cagttttaat tgtggagggg
6901 aatttttcta ctgtaattca acacaactgt ttaatagtac ttggtttaat agtacttgga
6961 gtactgaagg gtcaaataac actgaaggaa gtgacacaat cacactccca tgcagaataa
7021 aacaatttat aaacatgtgg caggaagtag gaaaagcaat gtatgcccct cccatcagtg
7081 gacaaattag atgttcatca aatattactg ggctgctatt aacaagagat ggtggtaata
7141 acaacaatgg gtccgagatc ttcagacctg gaggaggcga tatgagggac aattggagaa
7201 gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg
7261 caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg
7321 ggttcttggg agcagcagga agcactatgg gctgcacgtc aatgacgctg acggtacagg
7381 ccagacaatt attgtctgat atagtgcagc agcagaacaa tttgctgagg gctattgagg
7441 cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa acagctccag gcaagaatcc
7501 tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa
7561 aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac
7621 agatttggaa taacatgacc tggatggagt gggacagaga aattaacaat tacacaagct
7681 taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat
7741 tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt
7801 atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg
7861 tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc
7921 tcccaatccc gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag
7981 acagagacag atccattcga ttagtgaacg gatccttagc acttatctgg gacgatctgc
8041 ggagcctgtg cctcttcagc taccaccgct tgagagactt actcttgatt gtaacgagga
8101 ttgtggaact tctgggacgc agggggtggg aagccctcaa atattggtgg aatctcctac
8161 agtattggag tcaggaacta aagaatagtg ctgttaactt gctcaatgcc acagccatag
8221 cagtagctga ggggacagat agggttatag aagtattaca agcagcttat agagctattc
8281 gccacatacc tagaagaata agacagggct tggaaaggat tttgctataa gatgggtggc
8341 aagtggtcaa aaagtagtgt gattggatgg cctgctgtaa gggaaagaat gagacgagct
8401 gagccagcag cagatggggt gggagcagta tctcgagacc tagaaaaaca tggagcaatc
8461 acaagtagca atacagcagc taacaatgct gcttgtgcct ggctagaagc acaagaggag
8521 gaagaggtgg gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca
8581 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
8641 caaagaagac aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat
8701 tggcagaact acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac
8761 aagctagtac cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc
8821 ttgttacacc ctgtgagcct gcatggaatg gatgaccctg agagagaagt gttagagtgg
8881 aggtttgaca gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc
8941 aagaactgct gacatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg
9001 cgtggcctgg gcgggactgg ggagtggcga gccctcagat gctgcatata agcagctgct
9061 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
9121 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
9181 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
9241 gaaaatctct agcacccagg aggtagaggt tgcagtgagc caagatcgcg ccaccattcg
9301 gggatcagag tttttaagga taacttagtg tgtagggggc cagtgagttg gagatgaaag
9361 cgtagggagt cgaaggtgtc cttttgcgcc gagtcagttc ctgggtgggg gccacaagat
9421 cggatgagcc agtttatcaa tccgggggtg ccagctgatc catggagtgc agggtctgca
9481 aaatatctca agcactgatt gatcttaggt tttacaatag tgatgttacc ccaggaacaa
9541 tttggggaag gtcagaatct tgtagcctgt agctgcatga ctcctaaacc ataatttctt
9601 ttttgttttt ttttttttat ttttgagaca gggtctcact ctgtcaccta ggctggagtg
9661 cagtggtgca atcacagctc actgcagcct caacgtcgta agctcaagcg atcctcccac
9721 ctcagcctgc ctggtagctg agactacaag cgacgcccca gttaattttt gtatttttgg
9781 tagaggcagc gttttgccgt gtggccctgg ctggtctcga actcctgggc tcaagtgatc
9841 cagcctcagc ctcccaaagt gctgggacaa ccgggcccag tcactgcacc tggccctaaa
9901 ccataatttc taatcttttg gctaatttgt tagtcctaca aaggcagtct agtccccagc
9961 aaaaaggggg tttgtttcgg gaaagggctg ttactgtctt tgtttcaaac tataaactaa
10021 gttcctccta aacttagttc ggcctacacc caggaatgaa caaggagagc ttggaggtta
10081 gaagcacgat ggaattggtt aggtcagatc tctttcactg tctgagttat aattttgcaa
10141 tggtggttca aagactgccc gcttctgaca ccagtcgctg cattaatgaa tcggccaacg
10201 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
10261 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
10321 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
10381 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
10441 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
10501 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
10561 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
10621 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
10681 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
10741 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
10801 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
10861 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
10921 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
10981 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
11041 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
11101 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
11161 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
11221 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
11281 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
11341 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
11401 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
11461 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
11521 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
11581 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
11641 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
11701 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
11761 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
11821 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
11881 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
11941 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
12001 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
12061 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
12121 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
12181 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
12241 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
12301 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
12361 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
12421 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc cagagtcctt
12481 ggtgtggagg gagggaccag cgcagcttcc agccatccac ctgatgaaca gaacctaggg
12541 aaagccccag ttctacttac accaggaaag gctggaaggg ctaatttggt cccaaaaaag
12601 acaagagatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
12661 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct tcaagttagt
12721 accagttgaa ccagagcaag tagaagaggc caatgaagga gagaacaaca gcttgttaca
12781 ccctatgagc cagcatggga tggaggaccc ggagggagaa gtattagtgt ggaagtttga
12841 cagcctccta gcatttcgtc acatggcccg agagctgcat
//
