| 质粒名称: | JME4390 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 129656 |
| 存储实验室: | Tristan Rossignol |
|
载体骨架基本信息 |
|
| 载体名称: | pSB1A3 |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 2000 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 酵母表达,CRISPR |
| 筛选标记: | LEU2 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | JME4390 was a gift from Tristan Rossignol (Addgene plasmid # 129656 ; http://n2t.net/addgene:129656 ; RRID:Addgene_129656) |
| 参考文献部分: | A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting wild-type strain diversity. Larroude M, Trabelsi H, Nicaud JM, Rossignol T. Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi: 10.1007/s10529-020-02805-4. 10.1007/s10529-020-02805-4 PubMed 31974649 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS JME4390 12297 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION LEU2ex_CrisprCas9-yl_RFP: CAS9 vector with LEU2ex marker for gRNA
cloning using GoldenGate.
ACCESSION .
VERSION .
KEYWORDS JME4390
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12297)
AUTHORS Larroude M, Trabelsi H, Nicaud JM, Rossignol T
TITLE A set of Yarrowia lipolytica CRISPR/Cas9 vectors for exploiting
wild-type strain diversity.
JOURNAL Biotechnol Lett. 2020 Jan 23. pii: 10.1007/s10529-020-02805-4. doi:
10.1007/s10529-020-02805-4.
PUBMED 31974649
REFERENCE 2 (bases 1 to 12297)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12297
/organism="synthetic DNA construct"
/mol_type="other DNA"
terminator 12..69
/label=his operon terminator
/note="This putative transcriptin terminator from the E.
coli his operon has a 2-bp deletion introduced during
synthesis. Its efficiency has not been determined."
primer_bind complement(93..110)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(264..852)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(344..363)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(1023..1883)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 1646..1665
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(1884..1988)
/gene="bla"
/label=AmpR promoter
terminator 2078..2121
/label=bacterial terminator
/note="putative bacterial transcription terminator"
protein_bind 2700..2721
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 2736..2766
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 2774..2790
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
RBS 2808..2819
/note="strong bacterial ribosome binding site (Elowitz and
Leibler, 2000)"
CDS 2826..3503
/codon_start=1
/product="monomeric derivative of DsRed (Campbell et al.,
2002)"
/label=mRFP1
/translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
TGA"
terminator 3548..3619
/label=rrnB T1 terminator
/note="transcription terminator T1 from the E. coli rrnB
gene"
terminator 3635..3662
/label=T7Te terminator
/note="phage T7 early transcription terminator"
protein_bind 4007..4040
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind complement(5910..5932)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind 6032..6051
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6245..6267
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 6259..6276
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 6260..6276
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
CDS 7889..11992
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 12005..12025
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
terminator 12036..12283
/gene="S. cerevisiae CYC1"
/label=CYC1 terminator
/note="transcription terminator for CYC1"
primer_bind complement(12052..12070)
/label=CYC1
/note="CYC1 transcription termination signal, reverse
primer"
ORIGIN
1 tcttcctgca gtccggcaaa aaagggcaag gtgtcaccac cctgcccttt ttctttaaaa
61 ccgaaaagat tacttcgcgt tatgcaggct tcctcgctca ctgactcgct gcgctcggtc
121 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
181 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
241 aaaaaggccg cgttgctggc gtttttccac aggctccgcc cccctgacga gcatcacaaa
301 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
361 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
421 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
481 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
541 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
601 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
661 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
721 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
781 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
841 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
901 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
961 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
1021 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
1081 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
1141 cccagtgctg caatgatacc gcgcgaccca cgctcaccgg ctccagattt atcagcaata
1201 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
1261 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
1321 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
1381 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
1441 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
1501 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
1561 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
1621 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
1681 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
1741 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
1801 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
1861 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
1921 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
1981 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
2041 acattaacct ataaaaatag gcgtatcacg aggcagaatt tcagataaaa aaaatcctta
2101 gctttcgcta aggatgattt ctggaattcg aagagcctct gacctgacgt ccccagttgc
2161 aaaagttgac acaactctag atctgcttcc aaatatagaa tcataacaag ggttagggtg
2221 tgattatata atattggtct taattgatgt gctagggctt taaaagttgg ttaaaataac
2281 gctctaatgc ctttttaata tattgtcttt ttcaaaatct caaatcggac acttcttcgt
2341 gtatgagact ccattttttg gctccgtcac gtgatatgta ttatcagcta tagtggtgta
2401 aacaaagttt tttactagct gtaatggcat tttgtcggag tggtaaatcg ccttcttgtt
2461 gtgcgttcga gttctggact ctgcactggg ctactttgaa aaatacctct aatgcgccga
2521 tggtttagtg gtaaaatcca tcgttgccat cgatgggccc ccggttcgag agacggggcc
2581 ggccatctgg gccctagagc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
2641 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
2701 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
2761 atgttgtgtg gaattgtgag cggataacaa tttcacacat actagagaaa gaggagaaat
2821 actagatggc ttcctccgaa gacgttatca aagagttcat gcgtttcaaa gttcgtatgg
2881 aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt cgtccgtacg
2941 aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg ttcgcttggg
3001 acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac ccggctgaca
3061 tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt gttatgaact
3121 tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac ggtgagttca
3181 tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt atgcagaaaa
3241 aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt gctctgaaag
3301 gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct gaagttaaaa
3361 ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc gacatcaaac
3421 tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa cgtgctgaag
3481 gtcgtcactc caccggtgct taataacgct gatagtgcta gtgtagatcg ctactagagc
3541 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
3601 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
3661 cgtttatata ggccatctgg gccgggggcg tctctgagct agaaatagca agttaaaata
3721 aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tttacgtcta
3781 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggcccagatc
3841 ctctagagtc gaagcggccg ctatgtctga taaaaggatg taacataggc aagctgctcg
3901 tgagtgttga gtacgaacct tagatccaaa tcacccgcac ccacggatat acttgcttga
3961 atatacagta gtatgcggcc gctaggtatt accctgttat ccctacataa cttcgtatag
4021 catacattat acgaagttat tctgaattcc gcctgagtca tcatttattt accagttggc
4081 cacaaaccct tgacgatctc gtatgtcccc tccgacatac tcccggccgg ctgggtacgt
4141 tcgatagcgc tatcggcatc gacaaggttt gggtccctag ccgataccgc actacctgag
4201 tcacaatctt cggaggttta gtcttccaca tagcacgggc aaaagtgcgt atatatacaa
4261 gagcgtttgc cagccacaga ttttcactcc acacaccaca tcacacatac aaccacacac
4321 atccacaatg gaacccgaaa ctaagaagac caagactgac tccaagaaga ttgttcttct
4381 cggcggcgac ttctgtggcc ccgaggtgat tgccgaggcc gtcaaggtgc tcaagtctgt
4441 tgctgaggcc tccggcaccg agtttgtgtt cgaggaccga ctcattggag gagctgccat
4501 tgagaaggag ggcgagccca tcaccgacgc tactctcgac atctgccgaa aggctgactc
4561 tattatgctc ggtgctgtcg gaggcgctgc caacaccgta tggaccactc ccgacggacg
4621 aaccgacgtg cgacccgagc agggcctcct caagctgcga aaggacctga acctgtacgc
4681 caacctgcga ccctgccagc tgctgtcgcc caagctcgcc gatctctccc ccatccgaaa
4741 cgttgagggc accgacttca tcattgtccg agagctcgtc ggaggtatct actttggaga
4801 gcgaaaggag gatgacggat ctggcgtcgc ttccgacacc gagacttact ccgttcctga
4861 ggttgagcga attgcccgaa tggccgcctt cctggccctt cagcacaacc cccctcttcc
4921 cgtgtggtcc cttgacaagg ccaacgtgct ggcctcctct cgactttggc gaaagactgt
4981 cacccgagtc ctcaaggacg agttccccca gctggagctc aaccaccagc tgatcgactc
5041 ggccgccatg atcctcatca agcagccctc caagatgaat ggtatcatca tcaccaccaa
5101 catgtttggc gatatcatct ccgacgaggc ctccgtcatc cccggttctc tgggtctgct
5161 gccctccgcc tctctggctt ctctgcccga caccaacgag gcgttcggtc tgtacgagcc
5221 ctgtcacgga tctgcccccg atctcggcaa gcagaaggtc aaccccattg ccaccattct
5281 gtctgccgcc atgatgctca agttctctct taacatgaag cccgccggtg acgctgttga
5341 ggctgccgtc aaggagtccg tcgaggctgg tatcactacc gccgatatcg gaggctcttc
5401 ctccacctcc gaggtcggag actttgttgc caacaaggtc aaggagctgc tcaagaagga
5461 gtaagtcgtt tctacgacgc attgatggaa ggagcaaact gacgcgcctg cgggttggtc
5521 taccggcagc agccctgcta atgaaatgat gatttataat ttaccggtgt agcaaccttg
5581 actagaagaa gcagattggg tgtgtttgta gtggaggaca gtggtacgtt ttggaaacag
5641 tcttcttgaa agtgtcttgt ctacagtata ttcactcata acctcaatag ccaagggtgt
5701 agtcggttta ttaaaggaag ggagttgtgg ctgatgtgga tagatatctt taaagctggc
5761 gactgcaccc aacgagtgtg gtggtagctt gttactgtat attcgaattc gtataacttc
5821 gtatagcagg agttatccga agcgataatt accctgttat ccctaacggg gccctttcgt
5881 cacgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggtgacggtc
5941 acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
6001 gttggcgggt gtcggggctg gcttaactat gcggcatcag agcagattgt actgagagtg
6061 caccatatgc ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc
6121 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
6181 ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat taagttgggt aacgccaggg
6241 ttttcccagt cacgacgttg taaaacgacg gccagtgaat tcgagctcgg tacccgcttt
6301 tcgtagataa tggaatacaa atggatatcc agagtataca catggatagt atacactgac
6361 acgacaattc tgtatctctt tatgttaact actgtgaggc gttaaataga gcttgatata
6421 taaaatgtta catttcacag tctgaacttt tgcagattac ctaatttggt aagatattaa
6481 ttatgaactg aaagttgatg gcatccctaa atttgatgaa agggggaacc cccgggttcg
6541 aaggtaccaa ggaagcatgc ggtacccgaa ttcgcttcga tacgcgtatc gatgatacgc
6601 gtccatggtg aggtgtctca caagtgccgt gcagtcccgc ccccacttgc ttctctttgt
6661 gtgtagtgta cgtacattat cgagagggtt gttcccgccc acctcgatcc ggcatgctga
6721 ggtgtctcac aagtgccgtg cagtcccgcc cccacttgct tctctttgtg tgtagtgtac
6781 gtacattatc gagagggttg ttcccgccca cctcgatccg gcatgctgag gtgtctcaca
6841 agtgccgtgc agtcccgccc ccacttgctt ctctttgtgt gtagtgtacg tacattatcg
6901 agagggttgt tcccgcccac ctcgatccgg catgctgagg tgtctcacaa gtgccgtgca
6961 gtcccgcccc cacttgcttc tctttgtgtg tagtgtacgt acattatcga gagggttgtt
7021 cccgcccacc tcgatccggc atgctgaggt gtctcacaag tgccgtgcag tcccgccccc
7081 acttgcttct ctttgtgtgt agtgtacgta cattatcgag agggttgttc ccgcccacct
7141 cgatccggca tgctgaggtg tctcacaagt gccgtgcagt cccgccccca cttgcttctc
7201 tttgtgtgta gtgtacgtac attatcgaga gggttgttcc cgcccacctc gatccggcat
7261 gctgaggtgt ctcacaagtg ccgtgcagtc ccgcccccac ttgcttctct ttgtgtgtag
7321 tgtacgtaca ttatcgagag ggttgttccc gcccacctcg atccgacatg ttgaggtgtc
7381 tcacaagtgc cgtgcagtcc cgcccccact tgcttctctt tgtgtgtagt gtacgtacat
7441 tatcgagagg gttgttcccg cccacctcga tccggcatgc actgatcacg ggcaaaagtg
7501 cttcgataga gagggggttg gcggcgcatt tgtgtcccaa aaaacagccc caattgcccc
7561 aattgacccc aaattgaccc agtagcggac ccaaccccgg cgagagcccc cttcacccca
7621 catatcaaac ctcccccggt tcccacactt gccgttaagg gcgtagggta ctgcagtctg
7681 gaatctacgc ttgttcagac tttgtactag tttctttgtc tggccatccg ggtaacccat
7741 gccggacgca aaatagacta ctgaaaattt ttttgctttg tggttgggac tttagccaag
7801 ggtataaaag accaccgtcc ccgaattacc tttcctcttc ttttctctct ctccttgtca
7861 actcacaccc gaaggatcca caaccacaat ggataagaaa tactccattg gcctggacat
7921 cggaaccaac tccgtgggtt gggccgtgat caccgatgag tacaaggtgc cctctaagaa
7981 attcaaggtc ctgggcaaca ccgaccgaca ctccatcaag aagaacctga tcggcgctct
8041 gctcttcgac tctggcgaga ctgctgaggc cacccgactg aagcgaaccg ctcgaagacg
8101 atacacccga agaaagaacc gaatctgtta cctgcaggag atcttctcta acgagatggc
8161 caaggtggac gactctttct tccaccgact ggaggagtct ttcctggtgg aggaggacaa
8221 gaagcacgag cgacacccca tcttcggcaa catcgtggac gaggtggcct accacgagaa
8281 gtaccccacc atctaccacc tgcgaaagaa gctggtggac tctaccgaca aggccgacct
8341 gcgactgatc tacctggccc tggcccacat gatcaagttc cgaggccact tcctgatcga
8401 gggcgacctg aaccccgaca actctgacgt ggacaagctg ttcatccagc tggtgcagac
8461 ctacaaccag ctcttcgaag agaaccccat taacgcttct ggcgtggatg ctaaggccat
8521 cctgtctgcc cgactgtcta agtctcgacg actcgagaac ctgattgctc agctccccgg
8581 agagaagaag aacggtctgt tcggaaacct gattgctctg tccctgggtc tgacccctaa
8641 cttcaagtcc aacttcgatc tggctgagga cgctaagctg cagctgtcta aggacaccta
8701 cgacgatgac ctggataacc tgctcgccca gattggcgac cagtacgccg acctgttcct
8761 ggccgccaag aacctgtctg acgccatcct gctgtctgac atcctgcgag tgaacaccga
8821 gatcaccaag gcccccctgt ctgcctccat gattaagcga tacgatgagc accaccagga
8881 tctgaccctc ctcaaggctc tggtccgaca gcagctgccc gagaagtaca aggagatttt
8941 cttcgaccag tctaagaacg gctacgccgg ctacatcgac ggcggcgcct ctcaggagga
9001 gttctacaag ttcattaagc ccatcctgga gaagatggac ggaaccgagg aactgctcgt
9061 gaagctgaac cgagaggacc tcctgcgaaa gcagcgaacc ttcgacaacg gctctatccc
9121 ccaccagatc cacctgggcg agctgcacgc catcctgcga cgacaggagg acttctaccc
9181 cttcctgaag gacaaccgag agaagatcga gaagatcctg accttccgaa tcccctacta
9241 cgtgggaccc ctggcccgag gaaactctcg attcgcttgg atgacccgaa agtctgagga
9301 gactattacc ccctggaact tcgaggaggt ggtggataag ggcgcctctg ctcagtcttt
9361 catcgagcga atgaccaact tcgacaagaa cctccccaac gagaaggtcc tgcccaagca
9421 ctctctgctc tacgagtact tcaccgtcta caacgagctc accaaggtca agtacgtgac
9481 cgagggaatg cgaaagcccg ctttcctgtc tggagagcag aagaaggcta ttgtggatct
9541 gctcttcaag actaaccgaa aggtcaccgt caagcagctg aaggaggatt acttcaagaa
9601 gattgagtgt ttcgattctg tcgagatctc cggcgtcgag gaccgattca acgcctctct
9661 gggtacctac cacgacctgc tgaagattat caaggacaag gatttcctgg ataacgagga
9721 gaacgaggat attctcgagg acattgtcct gaccctcacc ctgttcgagg atcgagagat
9781 gattgaggag cgactcaaga cctacgctca cctgttcgac gacaaggtga tgaagcagct
9841 gaagcgacga cgatacaccg gctggggccg actgtctcga aagctgatca acggcatccg
9901 agacaagcag tctggcaaga ccatcctgga cttcctgaag tctgacggct tcgccaaccg
9961 aaacttcatg cagctgatcc acgacgactc tctgaccttc aaggaggaca tccagaaggc
10021 ccaggtgtct ggccagggcg actctctgca cgagcacatc gccaacctgg ccggctctcc
10081 cgccattaag aaaggtatcc tgcagaccgt caaggtggtc gatgagctcg tcaaggtgat
10141 gggccgacac aagcccgaga acattgtcat tgagatggct cgagagaacc agactactca
10201 gaagggtcag aaaaactccc gagagcgaat gaagcgaatt gaggaaggta ttaaggagct
10261 gggctcccag attctcaagg agcatcccgt ggagaacact cagctccaga acgagaagct
10321 gtacctgtac tatctgcaga acggtcgaga catgtacgtc gaccaggagc tggatatcaa
10381 ccgactctcc gactacgatg tggaccacat tgtgccccag tccttcctga aggacgattc
10441 tatcgataac aaggtgctga cccgatccga caagaaccga ggcaagtctg acaacgtgcc
10501 ctccgaggag gtggtcaaga agatgaagaa ctactggcga cagctgctga acgccaagct
10561 gattacccag cgaaagttcg acaacctgac caaggccgag cgaggcggcc tgtctgagct
10621 ggacaaggcc ggcttcatca agcgacagct ggtggagact cgacagatca ccaagcacgt
10681 ggcccagatc ctggactctc gaatgaacac caagtacgac gagaacgaca agctgatccg
10741 agaggtgaag gtgatcaccc tgaagtctaa gctggtgtct gacttccgaa aggacttcca
10801 gttctacaag gtgcgagaga ttaacaacta ccaccacgcc cacgatgcct acctgaacgc
10861 tgtcgtgggc accgccctca tcaagaagta tcccaagctg gagtccgagt tcgtctacgg
10921 cgactacaag gtctacgatg tgcgaaaaat gattgccaag tccgagcagg agattggcaa
10981 ggctaccgcc aagtacttct tctactccaa cattatgaac ttcttcaaga ccgagattac
11041 cctggctaac ggcgagattc gaaagcgacc cctcattgag actaacggag agactggtga
11101 gatcgtgtgg gacaagggac gagacttcgc caccgtgcga aaggtgctgt ctatgcccca
11161 ggtgaacatc gtgaagaaga ccgaggtgca gaccggaggt ttctctaagg agtccatcct
11221 gcccaagcga aactctgaca agctgatcgc ccgaaagaag gactgggacc ccaagaagta
11281 cggaggtttc gactctccca ccgtggctta ctctgtgctg gtggtggcca aggtggagaa
11341 gggcaagtct aagaagctga agtctgtgaa ggagctgctg ggcattacca tcatggagcg
11401 atcttctttc gagaagaacc ccattgactt cctggaggcc aagggataca aggaggtgaa
11461 gaaagatctg attatcaagc tccccaagta ctctctgttc gagctggaga acggacgaaa
11521 gcgaatgctg gcctctgccg gcgagctgca gaagggaaac gagctggccc tgccctccaa
11581 gtacgtcaac ttcctgtacc tcgcctccca ttacgagaag ctgaagggct ctcccgagga
11641 taacgagcag aagcagctct tcgtggagca gcataagcac tacctggacg agatcatcga
11701 gcagatttct gagttctcta agcgagtgat cctggctgac gccaacctgg ataaggtgct
11761 gtctgcttac aacaagcacc gagacaagcc cattcgagag caggctgaga acatcattca
11821 cctgttcacc ctgaccaacc tgggagcccc cgctgccttc aagtacttcg acaccaccat
11881 cgaccgaaag cgatacacct ctaccaagga ggtgctggac gccaccctga tccaccagtc
11941 tatcaccggc ctgtacgaga ctcgaatcga cctgtctcag ctgggcggcg actctcgagc
12001 cgaccccaag aagaagcgaa aggtgtaagc tagcctcatg taattagtta tgtcacgctt
12061 acattcacgc cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga
12121 agtctaggtc cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt
12181 tcaaattttt cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac
12241 cttgcttgag aaggttttgg gacgctcgaa ggctttaatt tgcaagcttg gcggtct
//
