| 质粒名称: | FU-tet-o-hOct4 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 19778 |
| 存储实验室: | Konrad Hochedlinger |
|
载体骨架基本信息 |
|
| 载体名称: | FUdeltaGW |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳动物表达，慢病毒 |
| 筛选标记: | -- |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Oct4 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 1100 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI（销毁后未知） |
| 3' 克隆位点: | EcoRI（销毁后未知） |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | FU-tet-o-hOct4 was a gift from Konrad Hochedlinger (Addgene plasmid # 19778 ; http://n2t.net/addgene:19778 ; RRID:Addgene_19778) |
| 参考文献部分: | A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS FU-tet-o-hOct4 9471 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FU-tet-o-hOct4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9471)
AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger
K
TITLE A high-efficiency system for the generation and study of human
induced pluripotent stem cells.
JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
PUBMED 18786420
REFERENCE 2 (bases 1 to 9471)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..9471
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2610..2880
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2913..2950
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2947..2971
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 3050..3059
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 4163..4751
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4216..4236)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4634..4645)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4754..4770)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4755..4771)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5276..5456
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5482..5499)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5488..5712
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 5758..6186
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5845..5864)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6055..6076
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6195..6215)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6200..6529
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6380..6515
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6442..6461
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 6577..6624
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6643..7017
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7147..7268
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7184..7203)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7238..7257
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7317..7333)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7317..7333)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7330..7352)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7341..7357
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7365..7395)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7410..7431
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7548..7565)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(7719..8307)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(7799..7818)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8478..9338)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9101..9120
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9339..9443)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg ccaccatggc
3061 gggacacctg gcttcggatt tcgccttctc gccccctcca ggtggtggag gtgatgggcc
3121 aggggggccg gagccgggct gggttgatcc tcggacctgg ctaagcttcc aaggccctcc
3181 tggagggcca ggaatcgggc cgggggttgg gccaggctct gaggtgtggg ggattccccc
3241 atgccccccg ccgtatgagt tctgtggggg gatggcgtac tgtgggcccc aggttggagt
3301 ggggctagtg ccccaaggcg gcttggagac ctctcagcct gagggcgaag caggagtcgg
3361 ggtggagagc aactccgatg gggcctcccc ggagccctgc accgtcaccc ctggtgccgt
3421 gaagctggag aaggagaagc tggagcaaaa cccggaggag tcccaggaca tcaaagctct
3481 gcagaaagaa ctcgagcaat ttgccaagct cctgaagcag aagaggatca ccctgggata
3541 tacacaggcc gatgtggggc tcaccctggg ggttctattt gggaaggtat tcagccaaac
3601 gaccatctgc cgctttgagg ctctgcagct tagcttcaag aacatgtgta agctgcggcc
3661 cttgctgcag aagtgggtgg aggaagctga caacaatgaa aatcttcagg agatatgcaa
3721 agcagaaacc ctcgtgcagg cccgaaagag aaagcgaacc agtatcgaga accgagtgag
3781 aggcaacctg gagaatttgt tcctgcagtg cccgaaaccc acactgcagc agatcagcca
3841 catcgcccag cagcttgggc tcgagaagga tgtggtccga gtgtggttct gtaaccggcg
3901 ccagaagggc aagcgatcaa gcagcgacta tgcacaacga gaggattttg aggctgctgg
3961 gtctcctttc tcagggggac cagtgtcctt tcctctggcc ccagggcccc attttggtac
4021 cccaggctat gggagccctc acttcactgc actgtactcc tcggtccctt tccctgaggg
4081 ggaagccttt ccccctgtct ctgtcaccac tctgggctct cccatgcatt caaactgaga
4141 attcgatatc aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac
4201 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
4261 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
4321 gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt
4381 gtttgctgac gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg
4441 gactttcgct ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg
4501 ctgctggaca ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc
4561 atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt
4621 ctgctacgtc ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc
4681 tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc
4741 cgcctccccg catcgatacc gtcgacctcg agacctagaa aaacatggag caatcacaag
4801 tagcaataca gcagctacca atgctgattg tgcctggcta gaagcacaag aggaggagga
4861 ggtgggtttt ccagtcacac ctcaggtacc tttaagacca atgacttaca aggcagctgt
4921 agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg
4981 aagacaagat atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattggca
5041 gaactacaca ccagggccag ggatcagata tccactgacc tttggatggt gctacaagct
5101 agtaccagtt gagcaagaga aggtagaaga agccaatgaa ggagagaaca cccgcttgtt
5161 acaccctgtg agcctgcatg ggatggatga cccggagaga gaagtattag agtggaggtt
5221 tgacagccgc ctagcatttc atcacatggc ccgagagctg catccggact gtactgggtc
5281 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
5341 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
5401 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagggc
5461 ccgtttaaac ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
5521 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
5581 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
5641 tggggcagga cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg
5701 tgggctctat ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg
5761 cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta
5821 cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt
5881 tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg
5941 ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat
6001 cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac
6061 tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag
6121 ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg
6181 cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc
6241 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc
6301 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt
6361 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
6421 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct
6481 attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg
6541 agcttgtata tccattttcg gatctgatca gcacgtgttg acaattaatc atcggcatag
6601 tatatcggca tagtataata cgacaaggtg aggaactaaa ccatggccaa gttgaccagt
6661 gccgttccgg tgctcaccgc gcgcgacgtc gccggagcgg tcgagttctg gaccgaccgg
6721 ctcgggttct cccgggactt cgtggaggac gacttcgccg gtgtggtccg ggacgacgtg
6781 accctgttca tcagcgcggt ccaggaccag gtggtgccgg acaacaccct ggcctgggtg
6841 tgggtgcgcg gcctggacga gctgtacgcc gagtggtcgg aggtcgtgtc cacgaacttc
6901 cgggacgcct ccgggccggc catgaccgag atcggcgagc agccgtgggg gcgggagttc
6961 gccctgcgcg acccggccgg caactgcgtg cacttcgtgg ccgaggagca ggactgacac
7021 gtgctacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt
7081 ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc
7141 caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
7201 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
7261 gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca
7321 tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga
7381 agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg
7441 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc
7501 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
7561 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
7621 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
7681 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
7741 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
7801 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
7861 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
7921 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
7981 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
8041 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
8101 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
8161 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
8221 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
8281 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
8341 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
8401 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
8461 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
8521 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
8581 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
8641 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
8701 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
8761 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
8821 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
8881 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
8941 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
9001 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
9061 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
9121 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
9181 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
9241 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
9301 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
9361 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
9421 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga c
//
