| 质粒名称: | pOpen-TthpolA |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 165533 |
| 存储实验室: | Drew Endy, Jennifer Molloy, FreeGenes Project |
|
载体骨架基本信息 |
|
| 载体名称: | pBuild |
| 载体出品公司: | Synthetic Biology |
| 完整质粒大小: | 4628bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Tth DNA polymerase |
| 物种来源: | 其他 |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | AATG (unknown if destroyed) |
| 3’克隆位点: | GCTT (unknown if destroyed) |
| 5’测序引物 | M13 forward |
| 3’测序引物 | M13 reverse |
|
文献引用方法 |
|
| 材料和方法部分: | pOpen-TthpolA was a gift from Drew Endy & Jennifer Molloy & FreeGenes Project (Addgene plasmid # 165533 ; http://n2t.net/addgene:165533 ; RRID:Addgene_165533) |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pOpen-TthpolA 4628 bp ds-DNA circular SYN 05-AUG-2021
DEFINITION DNA Polymerase whose activity is resistant to prolonged incubations
at high temperatures (95 degrees C)..
ACCESSION .
VERSION .
KEYWORDS pOpen-TthpolA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4628)
TITLE Open Enzyme Collection
REFERENCE 2 (bases 1 to 4628)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 5, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..4628
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(2651..2669)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 2665..2712
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
primer_bind complement(2717..2733)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(2717..2733)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
rep_origin complement(2800..3388)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(2880..2899)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(3579..4439)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 4202..4221
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(4440..4544)
/gene="bla"
/label=AmpR promoter
primer_bind 4560..4576
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
terminator 4577..4608
/label=tonB terminator
/note="bidirectional E. coli tonB-P14 transcription
terminator"
ORIGIN
1 aaaggggccg tcaatatcag ggtctctaat ggaggctatg ctgccgttgt tcgaaccgaa
61 aggccgcgtg ctgctggttg atggccatca cctggcctac cgtaccttct ttgcgctgaa
121 ggggctgact acttctcgtg gtgaaccggt gcaagccgtg tacggtttcg ccaaatccct
181 gctgaaagct ctgaaagagg acggctacaa agcggtattc gtggtattcg atgcgaaagc
241 gccgtctttt cgtcatgaag cgtacgaagc ttacaaagca ggccgtgctc cgacccctga
301 ggacttcccg cgtcagctgg ccctgattaa agaactggtc gacctgctgg gtttcacccg
361 tctggaggtt ccgggctacg aagccgatga cgtcctggca actctcgcta aaaaagctga
421 aaaagaaggt tatgaagttc gtatcctgac ggccgaccgc gacctgtacc agctggtttc
481 tgaccgtgtg gctgtgttgc acccggaagg tcatctgatt accccggaat ggctttggga
541 aaaatacggt ctgcgtccgg aacagtgggt ggacttccgt gcgctggttg gcgatccgtc
601 cgacaacctg ccgggtgtta aaggcattgg tgaaaaaacg gctctcaaac tgctgaaaga
661 gtgggggtcc ctggagaacc tgctgaaaaa cttggatcgc gtaaaaccgg agaacgtccg
721 tgaaaaaatt aaagcgcacc tggaggacct gcgtctgtcc ctggagctct ctcgtgtgcg
781 cactgacctc ccgctggaag tggatctcgc tcagggtcgt gaaccggacc gtgagggcct
841 gcgtgcgttt ctggaaagac tggagtttgg ttccctcctg cacgagttcg gcctgctcga
901 agcgccggca ccgctcgaag aggctccttg gccgccgccg gaaggcgctt tcgtaggctt
961 cgttctgagc cgtccggagc cgatgtgggc agaacttaag gcgctggctg cttgccgtga
1021 tggtcgcgtc caccgtgccg cggacccgct ggcagggctg aaagatttaa aagaagttcg
1081 cggtctgctg gctaaagacc tcgcagtgct ggcgagccgt gaaggactgg atctggtccc
1141 gggtgatgat ccgatgctgc tggcgtacct gctggacccg tccaacacca ccccggaagg
1201 cgttgcgcgc cgttacggcg gagaatggac cgaagatgca gctcatcgtg cgctgctgtc
1261 tgaacgcctg catcgtaacc tgctcaaacg cctggaaggc gaagaaaaac tgctgtggct
1321 ttatcacgaa gtggaaaaac ctctgtctcg tgtactggcc cacatggaag cgaccggcgt
1381 gcgtcgtgat gtggcttacc tgcaagccct gagcctggag ctggctgaag aaatccgccg
1441 tctggaagaa gaggtgttcc gtctggcggg tcatcctttt aacctgaaca gccgtgacca
1501 gctggaacgc gtcctgtttg acgaactgcg tctgccggct ctgggcaaga cccagaaaac
1561 cggcaaacgt agcacttccg ctgctgtgct tgaagcactg cgtgaggccc atccgatcgt
1621 cgaaaaaatc ctgcaacacc gtgaactgac gaaactgaaa aacacctacg tggacccgct
1681 gccgagcctg gtacatccgc gtaccggtcg gctgcacacc cgttttaatc agaccgctac
1741 cgcaaccggt cgtctttcta gctctgaccc gaacctgcaa aacatcccgg tacgtacccc
1801 gctgggtcag cgtatccgtc gtgctttcgt cgcggaagcc ggttgggcct tggtcgccct
1861 ggattatagt cagatcgaac tgcgtgtgct tgcgcacctg tctggcgacg aaaacttaat
1921 tcgtgtgttc caggagggta aagacatcca cacccagacg gcatcgtgga tgttcggcgt
1981 accgccggaa gcggttgacc cgctgatgcg ccgcgcagcc aaaaccgtta acttcggtgt
2041 cctgtacggt atgtctgcgc accgtctgtc ccaggagctg gcgatcccct acgaagaggc
2101 agttgcgttt atcgaacgtt actttcagag cttcccgaaa gtgcgtgctt ggatcgaaaa
2161 aaccctggaa gaaggtcgta aacgcggcta cgtcgaaacc ctgtttggtc gccgtcgtta
2221 tgtgccggac ctgaacgctc gtgtgaagag tgttcgtgaa gccgccgagc gtatggcgtt
2281 taacatgccg gttcagggga ccgcggctga cctgatgaaa ctggcaatgg tgaaactctt
2341 cccgcgtctg cgtgaaatgg gtgctcgcat gcttctgcaa gtgcacgatg aattgctcct
2401 ggaagctccg caggcgcgtg cggaggaagt ggcagccctt gcgaaagaag ctatggaaaa
2461 agcctacccg ctggcagttc cgctggaagt cgaagtgggt atgggtgaag attggctctc
2521 cgcgaaaggc tgaagagctt tgagaccact agattagcaa ggcacccgtg tgcaaacact
2581 tgcttctcta gggataacag ggtaatggga cagaggtcgt agtcgcccgc tgaggtgtca
2641 atcgtcggag ccgctgagca ataactagca taaccccttg gggcctctaa acgggtcttg
2701 aggggttttt tgcatggtca tagctgtttc ctgagagctt ggcaggtgat gacacacatt
2761 aacaaatttc gtgaggagtc tccagaagaa tgccattaat ttccataggc tccgcccccc
2821 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
2881 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
2941 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc
3001 acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga
3061 accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc
3121 ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag
3181 gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag
3241 aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag
3301 ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca
3361 gattacacgc agaaaaaaag gatctcaaga aggcctacta ttagcaacaa cgatcctttg
3421 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
3481 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
3541 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
3601 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
3661 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
3721 gaaccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
3781 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
3841 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
3901 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
3961 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
4021 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
4081 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
4141 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
4201 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
4261 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
4321 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
4381 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
4441 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
4501 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacctg caccagtcag
4561 taaaacgacg gccagtagtc aaaagcctcc gaccggaggc ttttgacttg gttcaggtgg
4621 agtgggag
//
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