| 质粒名称: | pMIND |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 24730 |
| 存储实验室: | Brian Robertson |
|
载体骨架基本信息 |
|
| 载体名称: | pSHKLx |
| 载体出品公司: | -- |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 6078 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 细菌表达 |
| 筛选标记: | 潮霉素 |
| 高拷贝/低拷贝: | 低拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | TetRO promoter,TetRO |
| 物种来源: | C. glutamicum |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | XbaI(未破坏) |
| 3' 克隆位点: | BamHI(未破坏) |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pMIND was a gift from Brian Robertson (Addgene plasmid # 24730 ; http://n2t.net/addgene:24730 ; RRID:Addgene_24730) |
| 参考文献部分: | Tetracycline-inducible gene regulation in mycobacteria. Blokpoel MC, Murphy HN, O'Toole R, Wiles S, Runn ES, Stewart GR, Young DB, Robertson BD. Nucleic Acids Res. 2005 . 33(2):e22. 10.1093/nar/gni023 PubMed 15687380 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pMIND 6778 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS pMIND
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6778)
AUTHORS Blokpoel MC, Murphy HN, O'Toole R, Wiles S, Runn ES, Stewart GR,
Young DB, Robertson BD
TITLE Tetracycline-inducible gene regulation in mycobacteria.
JOURNAL Nucleic Acids Res. 2005 . 33(2):e22.
PUBMED 15687380
REFERENCE 2 (bases 1 to 6778)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6778
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 19..35
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 19..35
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(1916..1933)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(1916..1932)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(1925..1947)
/label=M13/pUC Forward
/note="In lacZ gene"
CDS 4745..5539
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4799..4818)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 5409..5428
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
rep_origin 5823..6411
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 6312..6331
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 6565..6582
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 6699..6720
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 6735..6765
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 6773..11
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 6778..22
/label=M13/pUC Reverse
/note="In lacZ gene"
ORIGIN
1 gcggataaca atttcacaca ggaaacagct atgacatgat tacgaattag cccgcctaat
61 gagcgggctt ttttttgaat tagcccgcct aatgagcggg cttttttttg aattcgagct
121 cggtacctct agatcacgat tcgctcgagg tcaatgacgc tgtgcgggag tgagagaatc
181 cgtcgatcag tgctgtcagg ccgaaatcga attcccggtc gcgttcagga acgctcgcat
241 tgccgacctc tgcggtttgc tcctccagta cgacaccgac ggtgtatcgg ctgaccgcga
301 tgagcatgct gatggctgtc tgttcatcga atccctgaga cacgagaagc ccgacttgct
361 gaccggaagc ctcagctcct tcgtcggtgt cgtcgccgga tggtctgcgg tggtgctcag
421 cgtggagacg cgcaccgtca cggaccgcgt gtagtgcgtg gcggaaactt cttgcgtttc
481 gcaagagaaa tgcgtcccat ttctcgtcgg actcggggaa ggaagcgtga tgctctcggt
541 caagcacgtc gctcgccagc gctgcgagga gttcggcctt cgtgcggaag tgccagtaga
601 ggccgggctg ctgtacctgt aagtgagccg ccagcgcgcg agtggtgaag ccatcgagcc
661 cagtctcgtc gagcacctgc cgggccccga gcaacacgga cgtgcggtcg agacgcttcc
721 ggtggtgagt catagttgca ctttatcatc gataacttta tcttagataa agtgactgct
781 cgctactctc atctgactgc tcgctactct catcgtggaa tcctgacagg atcccttaag
841 aactgcagtc catatggata tccttaatta agtatgcatc gacgcgttgt acaggactag
901 tgcatgcgtg acacaagaat ccctgttact tctcgaccgt attgattcgg atgattccta
961 cgcgagcctg cggaacgacc aggaattctg ggagccgctg gcccgccgag ccctggagga
1021 gctcgggctg ccggtgccgc cggtgctgcg ggtgcccggc gagagcacca accccgtact
1081 ggtcggcgag cccggcccgg tgatcaagct gttcggcgag cactggtgcg gtccggagag
1141 cctcgcgtcg gagtcggagg cgtacgcggt cctggcggac gccccggtgc cggtgccccg
1201 cctcctcggc cgcggcgagc tgcggcccgg caccggagcc tggccgtggc cctacctggt
1261 gatgagccgg atgaccggca ccacctggcg gtccgcgatg gacggcacga ccgaccggaa
1321 cgcgctgctc gccctggccc gcgaactcgg ccgggtgctc ggccggctgc acagggtgcc
1381 gctgaccggg aacaccgtgc tcacccccca ttccgaggtc ttcccggaac tgctgcggga
1441 acgccgcgcg gcgaccgtcg aggaccaccg cgggtggggc tacctctcgc cccggctgct
1501 ggaccgcctg gaggactggc tgccggacgt ggacacgctg ctggccggcc gcgaaccccg
1561 gttcgtccac ggcgacctgc acgggaccaa catcttcgtg gacctggccg cgaccgaggt
1621 caccgggatc gtcgacttca ccgacgtcta tgcgggagac tcccgctaca gcctggtgca
1681 actgcatctc aacgccttcc ggggcgaccg cgagatcctg gccgcgctgc tcgacggggc
1741 gcagtggaag cggaccgagg acttcgcccg cgaactgctc gccttcacct tcctgcacga
1801 cttcgaggtg ttcgaggaga ccccgctgga tctctccggc ttcaccgatc cggaggaact
1861 ggcgcagttc ctctgggggc cgccagacac cgcccccggc gcctaaaagc ttggcactgg
1921 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
1981 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
2041 cccaacagtt gcgcagcctg aatggcgaat ggcgataagc tagcccaacc agcccgcgaa
2101 cggctggcag ccagcgtaag gcgcggctca tcgggcggcg ttcgccacga tgtcctgcac
2161 ttcgagccaa gcctcgaaca cctgctggtg tgcacgactc acccggttgt tgacaccgcg
2221 cgcggccgtg cgggctcggt ggggcggctc tgtcgccctt gccagcgtga gtagcgcgta
2281 cctcacctcg cccaacaggt cgcacacagc cgattcgtac gccataaagc caggtgagcc
2341 caccagctcc gtaagttcgg gcgctgtgtg gctcgtaccc gcgcattcag gcggcagggg
2401 gtctaacggg tctaaggcgg cgtgtacggc cgccacagcg gctctcagcg gcccggaaac
2461 gtccttgaaa cgacgtatgt gttcctcctg gttggtacag gtggttgggg gtgctcggct
2521 gtcgctggtg ttccaccacc agggctcgac gggagagcgg gggagtgtgc agttgtgggg
2581 tggcccctca gcgaaatatc tgacttggag ctcgtgtcgg accatacacc ggtgattaat
2641 cgtggtctac taccaagcgt gagccacgtc gccgacgaat ttgagcagct ctggctgccg
2701 tactggccgc tggcaagcga cgatctgctc gaggggatct accgccaaag ccgcgcgtcg
2761 gccctaggcc gccggtacat cgaggcgaac ccaacagcgc tggcaaacct gctggtcgtg
2821 gacgtagacc atccagacgc agcgctccga gcgctcagcg cccgggggtc ccatccgctg
2881 cccaacgcga tcgtgggcaa tcgcgccaac ggccacgcac acgcagtgtg ggcactcaac
2941 gcccctgttc cacgcaccga atacgcgcgg cgtaagccgc tcgcatacat ggcggcgtgc
3001 gccgaaggcc ttcggcgcgc cgtcgacggc gaccgcagtt actcaggcct catgaccaaa
3061 aaccccggcc acatcgcctg ggaaacggaa tggctccact cagatctcta cacactcagc
3121 cacatcgagg ccgagctcgg cgcgaacatg ccaccgccgc gctggcgcca gcagaccacg
3181 tacaaagcgg ctccgacgcc gctagggcgg aattgcgcac tgttcgattc cgtcaggttg
3241 tgggcctatc gtcccgccct catgcggatc tacctgccga cccggaacgt ggacggactc
3301 ggccgcgcga tctatgccga gtgccacgtc cgaaacgccg agttcccgtg caacgacgtg
3361 tgtcccggac cgctaccgga cagcgaggtc cgcgccatcg ccaacagcat ttggcgttgg
3421 atcacaacca agtcgcgcat ttgggcggac gggatcgtgg tctacgaggc cacactcagt
3481 gcgcgccagt cggccatctc gcggaagggc gcagcagcgc gcacggcggc gagcacagtt
3541 gcgcggcgcg caaagtccgc gtcagccatg gaggcattgc tatgagcgac ggctacagcg
3601 acggctacaa ccggcagccg actgtccgca aaaagcggcg cgtgaccgcc gccgaaggcg
3661 ctcgaatcac cggactatcc gaacgccacg tcgtccggct cgtggcgcag gaacgcagcg
3721 agtggctcgc cgagcaggct gcacgccgcg aacgcatccg cgcctatcac gacgacgagg
3781 gccactcttg gccgcaaacg gccaaacatt tcgggctgca tctggacacc gttaagcgac
3841 tcggctatcg ggcgaggaaa gagcgtgcgg cagaacagga agcggctcaa aaggcccaca
3901 acgaagccga caatccaccg ctgttctaac gcaattgggg agcgggtgtc gcgggggttc
3961 cgtggggggt tccgttgcaa cgggtcggac aggtaaaagt cctggtagac gctagttttc
4021 tggtttgggc catgcctgtc tcgttgcgtg tttcgttgcg cccgttttga ataccagcca
4081 gacgagacgg ggttctacga atcttggtcg ataccaagcc atttccgctg aatatcgggg
4141 agctcaccgc cagaatcggt ggttgtggtg atgtacgtgg cgaactccgt tgtagtgcct
4201 gtggtggcat ccgtggccac tctcgttgca cggttcgttg tgccgttaca ggccccgttg
4261 acagctcacc gaacgtagtt aaaacatgct ggtcaaacta ggtttaccaa cgatacgagt
4321 cagctcatct agggccagtt ctaggcgttg ttcgttgcgc ggttcgttgc gcatgtttcg
4381 tgtggttgct agcttcacgc tgccgcaagc actcagggcg caagggctgc taaaggaagc
4441 ggaacacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact
4501 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc
4561 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag
4621 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttcttgc
4681 cgccaaggat ctgatggcgc agggatcaag atctgatcaa gagacaggat gaggatcgtt
4741 tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct
4801 attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg tgttccggct
4861 gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga
4921 actccaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc
4981 tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg
5041 gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc
5101 aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca
5161 tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga
5221 cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg cgcggatgcc
5281 cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata tcatggtgga
5341 aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca
5401 ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg
5461 cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct
5521 tcttgacgag ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgccc
5581 aacctgccat cacgagattt cgattccacc gccgccttct atgaaaggtt gggcttcgga
5641 atcgttttcc gggacgccgg ctggatgatc ctccagcgcg gggatctcat gctggagttc
5701 ttcgcccacc ccaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
5761 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
5821 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
5881 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
5941 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6001 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
6061 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6121 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6181 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6241 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6301 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
6361 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
6421 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
6481 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
6541 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
6601 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
6661 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
6721 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtga
//
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