LOCUS pLVX-Hom-Nuc1 8499 bp ds-DNA circular SYN 30-JUL-2013
COMMENT Created by Clontech Laboratories Inc. http://www.clontech.com
KEYWORDS pLVX-Hom-Nuc1 Vector
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8499)
AUTHORS Clontech Laboratories Inc.
FEATURES Location/Qualifiers
source 1..8499
/organism="synthetic DNA construct"
/mol_type="other DNA"
LTR 1..634
/label="5' LTR"
misc_feature 681..806
/label="Packaging signal"
misc_feature 1303..1536
/label="RRE"
misc_feature 2028..2144
/label="cPPT/CTS"
enhancer 2201..2504
/label="CMV enhancer"
promoter 2505..2708
/label="CMV promoter"
misc_feature 2803..2820
/label="5' MCS"
CDS 2824..2844
/label="SV40 NLS"
CDS 2854..3174
/label="DmrB"
misc_feature 3176..3189
/label="3' MCS"
misc_feature 3202..3775
/label="IRES"
CDS 3808..4407
/label="PuroR"
misc_feature 4421..5009
/label="WPRE"
LTR 5217..5850
/label="3' LTR"
rep_origin complement(6381..6966)
/direction=LEFT
/label="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(7137..7997)
/label="AmpR"
promoter complement(7998..8102)
/label="AmpR promoter"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
2161 acagcagaga tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa
2221 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
2281 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
2341 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
2401 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta cgggactttc
2461 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
2521 agtacaccaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
2581 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
2641 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
2701 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
2761 tccatagaag acaccgactc tactagagga tctatttccg gtgaattcct cgagactagt
2821 atgcctaaga agaagagaaa ggtggcttct agaggagtgc aggtggagac tatctcccca
2881 ggagacgggc gcaccttccc caagcgcggc cagacctgcg tggtgcacta caccgggatg
2941 cttgaagatg gaaagaaagt tgattcctcc cgggacagaa acaagccctt taagtttatg
3001 ctaggcaagc aggaggtgat ccgaggctgg gaagaagggg ttgcccagat gagtgtgggt
3061 cagagagcca aactgactat atctccagat tatgcctatg gtgccactgg gcacccaggc
3121 atcatcccac cacatgccac tctcgtcttc gatgtggagc ttctaaaact ggaaagcggc
3181 cgcggatcct acgtatgacc gcccctctcc ctcccccccc cctaacgtta ctggccgaag
3241 ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc
3301 ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg
3361 tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc
3421 tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc
3481 cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa
3541 ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct
3601 ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg
3661 atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg
3721 tctaggcccc ccgaaccacg gggacgtggt tttcctttga aaaacacgat gataagcttg
3781 ccacaaccca caaggagacg accttccatg accgagtaca agcccacggt gcgcctcgcc
3841 acccgcgacg acgtcccccg ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
3901 gccacgcgcc acaccgtcga cccggaccgc cacatcgagc gggtcaccga gctgcaagaa
3961 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
4021 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
4081 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
4141 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
4201 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
4261 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
4321 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
4381 tgcatgaccc gcaagcccgg tgcctagacg cgtctggaac aatcaacctc tggattacaa
4441 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
4501 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
4561 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
4621 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
4681 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
4741 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
4801 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
4861 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
4921 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
4981 tcggatctcc ctttgggccg cctccccgcc tggaattaat tctgcagtcg agacctagaa
5041 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
5101 gaagcacaag aggaggagga ggtgggtttt tccagtcaca cctcaggtac ctttaagacc
5161 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga
5221 agggctaatt cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca
5281 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
5341 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
5401 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
5461 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
5521 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
5581 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
5641 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
5701 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
5761 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
5821 ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt
5881 atttataact tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt
5941 ttaccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
6001 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
6061 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
6121 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
6181 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
6241 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
6301 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
6361 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
6421 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
6481 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
6541 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
6601 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
6661 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
6721 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
6781 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
6841 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
6901 accaccgctg gtagcggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
6961 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
7021 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
7081 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
7141 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
7201 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
7261 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
7321 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
7381 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
7441 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
7501 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
7561 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
7621 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
7681 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
7741 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
7801 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
7861 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
7921 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
7981 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
8041 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
8101 cgcacatttc cccgaaaagt gccacctgac gtcgacggat cgggagatca acttgtttat
8161 tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt
8221 tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg
8281 gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa cttcccaccc cataccctat
8341 taccactgcc aattacctag tggtttcatt tactctaaac ctgtgattcc tctgaattat
8401 tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta gtttttaaag
8461 aaattgtatt tgttaaatat gtactacaaa cttagtagt
//